Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	787,273	C→T	G108D (GGC→GAC)	gpmA ←	2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	787,273	0	C	T	100.0%	297.5 / NA	91	G108D (GGC→GAC)	gpmA	2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/52);  total (39/52)

CGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGCCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACG  >  NC_000913/787026‑787515                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   cgcACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCAt                                                                                                                                                                                                                                                 >  1:68757/1‑251 (MQ=255)     cGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTTATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACtt                                                                                                                                                                                                                                             >  1:151782/1‑251 (MQ=255)     cGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACtt                                                                                                                                                                                                                                             >  2:341927/1‑251 (MQ=255)     cGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACtt                                                                                                                                                                                                                                             >  2:522163/1‑251 (MQ=255)      gTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACttt                                                                                                                                                                                                                                            >  2:506014/1‑251 (MQ=255)      gTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACttt                                                                                                                                                                                                                                            >  1:18613/1‑251 (MQ=255)       tAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTGATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACtttt                                                                                                                                                                                                                                           >  2:375006/1‑251 (MQ=255)        aaaGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACtttt                                                                                                                                                                                                                                           <  1:20124/250‑1 (MQ=255)         aaGAGTTACAGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCAACTGCTCGTCGTCATACTTTTc                                                                                                                                                                                                                                          >  2:619508/1‑250 (MQ=255)           gagTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTcagc                                                                                                                                                                                                                                       <  2:72879/251‑1 (MQ=255)           gagTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTcagc                                                                                                                                                                                                                                       <  2:661821/251‑1 (MQ=255)               tACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGt                                                                                                                                                                                                                                    <  2:229803/250‑1 (MQ=255)                aCCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGtt                                                                                                                                                                                                                                   <  1:120762/250‑1 (MQ=255)                        cAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGGTTCCGCTTTg                                                                                                                                                                                                                          >  2:228922/1‑251 (MQ=255)                         aGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGt                                                                                                                                                                                                                         >  1:532982/1‑251 (MQ=255)                             atgatCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAg                                                                                                                                                                                                                     <  2:215497/251‑1 (MQ=255)                               gatCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGAc                                                                                                                                                                                                                   <  1:165358/251‑1 (MQ=255)                                   aCGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTg                                                                                                                                                                                                               <  1:412350/251‑1 (MQ=255)                                      cgcTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTcagca                                                                                                                                                                                                                                      <  2:314737/225‑1 (MQ=255)                                      cgcTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTcagca                                                                                                                                                                                                                                      >  1:314737/1‑225 (MQ=255)                                      cgcTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCAAGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTTTTTCTCGCTCAGTTTCGCGTAACCCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGACACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCa                                                                                                                                                                                                             >  1:444612/1‑250 (MQ=255)                                           aCCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGCTCACGCGGTCAATGGACAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGGTTGGCGTAACGCGGATCGTGACCCGGATAACGCCCATCATCTTTCGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCCCCTGCTCTTCGTCATACTTTTACGCAGTTTCCGCTTTGTTCAGACCATGCCACGCAc                                                                                                                                                                                                       >  2:34492/1‑251 (MQ=255)                                           aCCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCCCCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCCACGccc                                                                                                                                                                                                       >  2:537991/1‑249 (MQ=255)                                            ccGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCAcc                                                                                                                                                                                                      >  2:231013/1‑251 (MQ=255)                                                 ctTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGAAACGAGGATCGTGACCCGGATAACGCTCATCATATTTAGTCAGTTCCGGCGGCGTCAATGCAAAAACCA‑GACGCCACTGTTTCAACTGATCGTCGTAATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAgg                                                                                                                                                                                                 >  2:357156/1‑250 (MQ=255)                                                   tCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGt                                                                                                                                                                                                 <  1:342100/249‑1 (MQ=255)                                                    cATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGAc                                                                                                                                                                                              <  1:506014/251‑1 (MQ=255)                                                        cgcgGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGAcgttc                                                                                                                                                                                          >  2:396743/1‑251 (MQ=255)                                                           ggCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGAcgttcgtt                                                                                                                                                                                       >  2:556514/1‑251 (MQ=255)                                                            gCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACgttcgttc                                                                                                                                                                                      <  1:547570/251‑1 (MQ=255)                                                              aGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAg                                                                                                                                                                                    <  1:597037/251‑1 (MQ=255)                                                               gAATAGTTTCATTCCAGTCAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCCCTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGt                                                                                                                                                                                   >  1:523396/1‑251 (MQ=255)                                                                   aGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTcc                                                                                                                                                                               >  1:308156/1‑251 (MQ=255)                                                                          gtCCAGTCAGGGATCACGCGGTTTATGGTCAGCTCCAGGCTGGCCGTCATACGCAGTTCTTTCTCGCTCATTTTCGCGTAACGCGGATCGTGACCCGGAGAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGGCGCCTCTGTTTCACCTGCTCGTCGTCATACTTTTTAGCAGTTTCCGCTTTGTGCAGACCGGGCGACGCACAGTAATGACGTTCATTCTGTTTCCAGGAttt                                                                                                                                                                        <  2:139975/250‑1 (MQ=255)                                                                            ccAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTtc                                                                                                                                                                       <  1:388674/250‑1 (MQ=255)                                                                             cAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTGTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGTCCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCGTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTGTCCAGGATTtctc                                                                                                                                                                     <  2:47087/251‑1 (MQ=255)                                                                                    ggATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAG‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTGCAGGTTCCAGGATTTCTCAACGGGc                                                                                                                                                              <  2:108716/251‑1 (MQ=255)                                                                                             ggTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGcc                                                                                                                                                     >  2:165656/1‑251 (MQ=255)                                                                                                    ggTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGGTCAGTTTCCAGGATTTCTCAGCGGGCAGCCATGCCTGATCCa                                                                                                                                              <  2:261492/251‑1 (MQ=255)                                                                                                       cAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGt                                                                                                                                            <  1:318480/250‑1 (MQ=255)                                                                                                       cAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTAACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGt                                                                                                                                            >  2:318480/1‑250 (MQ=255)                                                                                                        aGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTc                                                                                                                                          <  1:534271/251‑1 (MQ=255)                                                                                                        aGCGCCAGGCTGTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTc                                                                                                                                          <  2:179452/251‑1 (MQ=255)                                                                                                                         cAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCcaca                                                                                                                         <  1:651965/251‑1 (MQ=255)                                                                                                                               cAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATGCCACAGGGTAt                                                                                                                   <  2:391257/251‑1 (MQ=255)                                                                                                                                      ttctCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAG‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAg                                                                                                             <  2:523396/250‑1 (MQ=255)                                                                                                                                      ttctCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAgc                                                                                                            >  1:355570/1‑251 (MQ=255)                                                                                                                                      gtctcGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCATCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGGTCAGTTTCCAGGATTTCTCAACGGGCAGCCAAGCCTGATCCAGTGCGTCCAGCACATTCCACAGGGTATGGATAg                                                                                                             <  1:640047/249‑1 (MQ=255)                                                                                                                                           gCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGtt                                                                                                       >  2:71722/1‑251 (MQ=255)                                                                                                                                            cTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGttt                                                                                                      >  2:150772/1‑251 (MQ=255)                                                                                                                                              cAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGttt                                                                                                      <  1:163619/249‑1 (MQ=255)                                                                                                                                              cAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTc                                                                                                     <  1:65535/250‑1 (MQ=255)                                                                                                                                                gTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGc                                                                                                  >  1:168683/1‑251 (MQ=255)                                                                                                                                                  ttCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGca                                                                                                 <  2:532982/250‑1 (MQ=255)                                                                                                                                                    cgcgTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAg                                                                                              <  2:411395/251‑1 (MQ=255)                                                                                                                                                     gcgNAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGa                                                                                             <  2:605881/251‑1 (MQ=255)                                                                                                                                                           cgcgGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAgtgt                                                                                        >  1:150121/1‑250 (MQ=255)                                                                                                                                                            gcgGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTaa                                                                                      <  1:79273/251‑1 (MQ=255)                                                                                                                                                              ggATCGTGACCCGGATAACTCTCATCATCTTTATTCAGTTCATGCGGAGTCACTTCAAAA‑CCACTGCGCCGCTGTTTCACCTGCTCGTCGTCATACTTGTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAGCGCACCGTAGTTACGTTCGTTCAGTTTCCAGGATTTCTCAACGGTCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCCCAGCGTATGGATAGCGCGTTTCAGCACAGGAGTGTAAGc                                                                                    <  2:444612/251‑1 (MQ=255)                                                                                                                                                              ggATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGc                                                                                    >  1:240307/1‑251 (MQ=255)                                                                                                                                                              ggATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGc                                                                                    <  2:55362/251‑1 (MQ=255)                                                                                                                                                                aTCGTGCCCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑ACACGACGCCACTGTTTCACCTGCTCGTCGTCATACTATTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCAAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCa                                                                                   >  2:413344/1‑250 (MQ=255)                                                                                                                                                                  cGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCaaa                                                                                 <  2:621037/250‑1 (MQ=255)                                                                                                                                                                        ccGGATAACGCTCATCAACTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTTCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTGCCAGGATGTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAg                                                                          <  2:414725/251‑1 (MQ=255)                                                                                                                                                                          ggATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCt                                                                        <  2:496084/251‑1 (MQ=255)                                                                                                                                                                              aaCGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTa                                                                     <  1:165656/250‑1 (MQ=255)                                                                                                                                                                                    catcatCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATTCTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGGGTAAGCAAAGTCAAAGCTGTAACCtt                                                                <  2:622578/249‑1 (MQ=255)                                                                                                                                                                                    catcatCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCtt                                                                <  1:537991/249‑1 (MQ=255)                                                                                                                                                                                     atcatcTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCa                                                                                                                                                                    <  2:491692/148‑1 (MQ=255)                                                                                                                                                                                     atcatcTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCa                                                                                                                                                                    >  1:491692/1‑148 (MQ=255)                                                                                                                                                                                            ttAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTc                                                        <  1:619508/249‑1 (MQ=255)                                                                                                                                                                                              aGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACCGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTcagc                                                     >  2:517003/1‑250 (MQ=255)                                                                                                                                                                                              aGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTcagc                                                     <  2:376724/250‑1 (MQ=255)                                                                                                                                                                                                     tCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCTCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGTGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTAc                                              <  1:627608/250‑1 (MQ=255)                                                                                                                                                                                                     tCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGCAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTAcc                                             >  2:45542/1‑251 (MQ=255)                                                                                                                                                                                                      ccGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTAcc                                             <  1:45542/250‑1 (MQ=255)                                                                                                                                                                                                       cggcggAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACctg                                           >  1:298947/1‑251 (MQ=255)                                                                                                                                                                                                         gcggAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACctgct                                         <  1:375006/251‑1 (MQ=255)                                                                                                                                                                                                           ggAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCtgctgc                                       >  2:487920/1‑251 (MQ=255)                                                                                                                                                                                                                    gCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATTGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCtt                               <  1:357156/250‑1 (MQ=255)                                                                                                                                                                                                                            cACGACGCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCCCCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACg                       >  2:223671/1‑251 (MQ=255)                                                                                                                                                                                                                               gacgCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCt                    >  2:171858/1‑251 (MQ=255)                                                                                                                                                                                                                                acgCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCtt                   <  1:311460/251‑1 (MQ=255)                                                                                                                                                                                                                                acgCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCtt                   <  2:150121/251‑1 (MQ=255)                                                                                                                                                                                                                                 cgCCACTGTTTCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCttt                  <  1:522163/251‑1 (MQ=255)                                                                                                                                                                                                                                           tCACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGCTCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTACGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACTCCTTTCTcagac           >  2:362167/1‑248 (MQ=255)                                                                                                                                                                                                                                            cACCTGCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGAt       <  1:150772/251‑1 (MQ=255)                                                                                                                                                                                                                                              ccTGCTCGTCGTCATAATTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGAACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCGTTTCTCAGACAGATcc     >  1:435228/1‑251 (MQ=255)                                                                                                                                                                                                                                                tGCTCGTCGTCATACTTTTAAGCCGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTGCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCAc   >  2:548750/1‑251 (MQ=255)                                                                                                                                                                                                                                                 gCTCGTCGTCATGCTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCAcg  <  1:54763/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gCTCGTCGTCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCAc   <  2:515790/250‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   CGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAA‑CCACGACGCCACTGTTTCACCTGCTCGTCGCCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGTTCAGTTTCCAGGATTTCTCAACGGGCAGCCATGCCTGATCCAGTTCGTCCAGCACATTCCACAGGGTATGGATAGCGCGTTTCAGCACAGAAGTGTAAGCAAAGTCAAAGCTGTAACCTTCCTCTTTCAGCAGCTTACCTGCTGCTTTTGCTTCGCTTACGCCTTTCTCAGACAGATCCACG  >  NC_000913/787026‑787515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑