Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,125,203	C→T	G42S (GGC→AGC)	mdtH ←	multidrug efflux pump MdtH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,125,203	0	C	T	100.0%	161.6 / NA	51	G42S (GGC→AGC)	mdtH	multidrug efflux pump MdtH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/30);  total (21/30)

ACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTGCATTGCCGCTAAGACTTTTCCCATCACTTCGGGG‑ACAATATTTTTTTATCAAAACGTCGCTTTGATAGAGA  >  NC_000913/1124956‑1125438                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             accaGCGCCGAACGCGGCGGCTCAAACAACGTGCCACCGAGGCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCAt                                                                                                                                                                                                                                           >  1:208428/1‑251 (MQ=255)    aGCGCCGAACGGGGCGGATCAAAAAACGTGCCACCGAGCCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGAGATACCCACTGTGGCGAATCCGGCGGCGTGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATCCCCAGCACTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTg                                                                                                                                                                                                                                        <  1:75888/251‑1 (MQ=255)           aaCGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACg                                                                                                                                                                                                                                 >  1:492554/1‑251 (MQ=255)                ggcggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGTCCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCg                                                                                                                                                                                                                            <  1:364124/251‑1 (MQ=255)                             acGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATGCCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGc                                                                                                                                                                                                               <  1:56885/251‑1 (MQ=255)                             acGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAAGCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGc                                                                                                                                                                                                               <  1:94867/251‑1 (MQ=255)                               gTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCgg                                                                                                                                                                                                             >  2:525782/1‑251 (MQ=255)                                tGCCACCGAGTCCCGAGAGCAGGCATGAAACCCCCAATAGCCCCGGTTCGTAGGCGATACCCATTGTGGCGAGGCCGGCGGCCCGGATCAGCATACCGGTAACAAGCATCGGTTTGGCACCAAAGCGGCCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATTACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCgg                                                                                                                                                                                                             <  2:1367/250‑1 (MQ=255)                                        aGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGacaac                                                                                                                                                                                                    <  1:535733/251‑1 (MQ=255)                                         gTCCCGAGAGCACGCATGAAAACCACAATAGCCACGGGTCGGGGGCGATCCCCATTGTGGCGACTCCGGCGGCGCGCATCAGCATACCGGTAACAATAATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACAGCCGAAAATACCCCGACCTTGCTGAATAACTTGGCGTAGACCGAGATCAATACCGACCATGCCAGCGGCCCAGCTCCTTTGATCAACCAATCGGATAGAGATCAGCGGGAAGacaac                                                                                                                                                                                                    >  2:146837/1‑250 (MQ=255)                                           cccGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACaaa                                                                                                                                                                                                 <  2:171960/251‑1 (MQ=255)                                               agagCAGGCATGAAAACCACACAAGCCACGGTTCGGGGGCGATACCCATTGAGACGAATCCAGCGGCGCGCATCAGCATACCGGTAACAATAATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATAACGAGACCTTGCTGACTAAATTGTCGTAGACCGAGAGCAATGCAGACCATGACGGCGGCCACGCTCATTTGATCAACGCAGCGGATATAGATAAACGGGAAGACAAAAAAGAAc                                                                                                                                                                                             >  2:365106/1‑251 (MQ=255)                                               agagCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCATCTCATTTGATCAACGAAGCGGATAGAGATCATCGGTAAGACAACAAAGAAc                                                                                                                                                                                             <  1:475657/251‑1 (MQ=255)                                                        aTGAAACCCACAAGTGCCACGGATCGGGGGAGATACCCATGGGGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAATAATCATCGGGTTGGCACCAAAGCGGTCGGACAGTGCAGCGCCGAAAATACCCAGACCTTGCTGAATAAAGTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATGTGAGCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAGCCCCAGCAc                                                                                                                                                                                     <  2:269991/250‑1 (MQ=255)                                                             aaCCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAg                                                                                                                                                                               <  1:525782/251‑1 (MQ=255)                                                              aCCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGc                                                                                                                                                                              >  1:311030/1‑251 (MQ=255)                                                                 acaATAGCCACGGGTCGTGGGCGATACCCATTGTGGCGAATCTGGCGGCGCGCATCAGACTCCCGGTCACAATAATCGGTTTGGAACCAAAGCGGTCGGCAATTGCACCGCCGGAAATACCCAGACCTTGCTGCATAAATCGGCGTAGACCTATAGCAGTACCGACCATGACAGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGCACACCAGCACGACCACCatc                                                                                                                                                                           >  2:556040/1‑250 (MQ=255)                                                                                   gggCGATACCCATGGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGaaa                                                                                                                                                         <  2:313646/251‑1 (MQ=255)                                                                                       gATACCCATTTTGTAGAATCCGGCGGCGCGCGTCATCATCCCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCCCCGCCGAAAAGACCCAGACCTTGCTGAATAAATTGGCGTAGACCGATAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGTATAGAGATCATCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATAtt                                                                                                                                                     <  2:474810/251‑1 (MQ=255)                                                                                        aTACCCATTGTGGCGAATCCGGCGGAGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAatat                                                                                                                                                      <  2:487140/249‑1 (MQ=255)                                                                                                               ggcgCGCCTCAGCATCCCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTTCGaca                                                                                                                             <  2:28159/251‑1 (MQ=255)                                                                                                               ggcgCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATAT‑ATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGacac                                                                                                                            >  2:533558/1‑251 (MQ=255)                                                                                                                 cgcccagcaggaTACCGGTAACAATAATCGTGTTGGCACTAAAGCTTATGGCAATTGTATCTCCGAAAATACCCAGTCATTGCTGAATAAATTGGCGTAGACCGAGAGCAATACAGACCATGACGGGGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGGGGGAAGACAACAAATATCCCCAGCACGTCCAGCATATTATCGATGAGCAGGAAATATTGCCGCAGTATCCTCGCCTGCGacccg                                                                                                                           <  2:433163/240‑4 (MQ=255)                                                                                                                        cAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGAc                                                                                                                     <  1:346430/250‑1 (MQ=255)                                                                                                                              aCCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACACCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTccc                                                                                                              >  1:586079/1‑251 (MQ=255)                                                                                                                                 ggTAACAATCATCGGTTTGGCCCCAAAGGGGTCGGCAAGTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCGCGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTc                                                                                                           <  2:29971/251‑1 (MQ=255)                                                                                                                                  gTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTcc                                                                                                          <  1:547785/251‑1 (MQ=255)                                                                                                                                       aaTCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCggg                                                                                                      >  1:68381/1‑250 (MQ=255)                                                                                                                                        atcatcGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATAT‑ATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTGCCCCTCCCGGGaaa                                                                                                   <  1:533558/251‑1 (MQ=255)                                                                                                                                            tcGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATa                                                                                                 >  2:197192/1‑250 (MQ=255)                                                                                                                                                   ggCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATg                                                                                          <  1:13969/250‑1 (MQ=255)                                                                                                                                                     cACNAAAGCGGTCGGCAATGGCACCGCCGAAAATAGCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTTCCCGCCCGGGAAATAAAAGATGAGc                                                                                       <  2:608844/251‑1 (MQ=255)                                                                                                                                                     cACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGGCCGAGAGCAATACCGACCATGACGGCGGTCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGTGCAGGAAATATTTACCCAGGTTCTTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGc                                                                                       <  1:426898/251‑1 (MQ=255)                                                                                                                                                      aCCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGc                                                                                       >  1:197750/1‑250 (MQ=255)                                                                                                                                                      aCCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGc                                                                                       >  1:344640/1‑250 (MQ=255)                                                                                                                                                        cAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGGCCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGTAAATAAAAGATGAGCACt                                                                                    <  2:98605/251‑1 (MQ=255)                                                                                                                                                           aGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGACCCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCAAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTc                                                                                 >  2:424409/1‑251 (MQ=255)                                                                                                                                                                  ggCAATTGAACCGGCGAAAAGACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAAGGCCGACCATGACGGCGGCCCAGCTCAGTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCTTGCGACACTCGGTACAGTTCGCCTCCAGGGAAATAAAAGATGAGCACTTTCGATTCTg                                                                          <  2:84756/251‑1 (MQ=255)                                                                                                                                                                  ggCAATTACACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGCAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGCAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTACTAGCCTGCGCCACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTg                                                                          >  1:471738/1‑251 (MQ=255)                                                                                                                                                                      aTTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTGCAtt                                                                      <  2:163635/251‑1 (MQ=255)                                                                                                                                                                         gCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTGCATTGcc                                                                   <  1:453362/251‑1 (MQ=255)                                                                                                                                                                         gCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTGCATTGc                                                                    <  2:392091/250‑1 (MQ=255)                                                                                                                                                                                    aaTACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTGCATTGCCGCTAAGACttt                                                        >  2:183558/1‑251 (MQ=255)                                                                                                                                                                                                 tGCTGAATAAATTGGCGTAGGCCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTGCATTGCCGCTAAGACTTTTCCCATCACTTc                                            <  2:85911/250‑1 (MQ=255)                                                                                                                                                                                                            ttGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTGCATTGCCGCTAAGACTTTTCCCATCACTTCGGGG‑ACAATAtt                               >  1:615932/1‑251 (MQ=255)                                                                                                                                                                                                                         cgagCAATACCAACCATGACGGCGGCTCCGATCATTTGATCAACGACGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAACACGAACAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGTGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTGAATTGCCGCTAAGACTTTTCCCATCACTTCGGGGTACA‑TATTTTTTTATCAAAAc                  >  2:356160/2‑251 (MQ=255)                                                                                                                                                                                                                           agCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTGCATTGCCGCTAAGACTTTTCCCATCACTTCGGGG‑ACAATATTTTTTTATCAAAACGt                >  2:210167/1‑251 (MQ=255)                                                                                                                                                                                                                            gCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTGCATTGCCGCTAAGACTTTTCCCATCACTTCGGGG‑ACAATATTTTTTTATCAAAACGTc               <  2:492554/251‑1 (MQ=255)                                                                                                                                                                                                                            gCAATACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGAGGAGCACTTTCTATTCTGCATTGCCGCTAAGACTTTTCCCATCACTTCGGGG‑ACAATATTTTTTTATCAAAACGTc               <  1:595530/251‑1 (MQ=255)                                                                                                                                                                                                                              aaTACCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTGCATTGCCGCTAAGACTTTTCCCATCACTTCGGGG‑ACAATATTTTTTTATCAAAACGTCGc             >  1:442991/1‑251 (MQ=255)                                                                                                                                                                                                                                 aCCGACCATGACGGCGGCCCAGCTCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTGCATTGCCGCTAAGACTTTTCCCATCACTTCGGGG‑ACAATATTTTTTTATCAAAACGTCGCttt          >  1:537275/1‑251 (MQ=255)                                                                                                                                                                                                                                         ggacaggcgcccagctcaTTTGATCAACGAAGCGGATAGAGAGCAGCGGGAAGACAACCAAGAACCCCAGCACGACCAGCATATTGTCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCATGCGACACGTGGGACAGTTCCCCTCCCGGGAAATAAAAGATGAGCGCTTTCTATTCTGCATGGCCGCTAAGACTTTTCCCATCACTTCGGGG‑ACAATATTTTTTTATCAAAACGTCGCTTTGATagaga  <  1:319557/243‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             ACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGACCTTGCTGAATAAATTGGCGTAGACCGAGAGCAATACCGACCATGACGGCGGCCCAGCCCATTTGATCAACGAAGCGGATAGAGATCAGCGGGAAGACAACAAAGAACCCCAGCACGACCAGCATATTATCGATGAGCAGGAAATATTTACCCAGGTTCCTCGCCTGCGACACGCGGGACATTTCCCCTCCCGGGAAATAAAAGATGAGCACTTTCTATTCTGCATTGCCGCTAAGACTTTTCCCATCACTTCGGGG‑ACAATATTTTTTTATCAAAACGTCGCTTTGATAGAGA  >  NC_000913/1124956‑1125438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑