Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,135,565	G→A	N269N (AAC→AAT)	pitB ←	metal phosphate:H(+) symporter PitB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,135,565	0	G	A	100.0%	266.5 / NA	76	N269N (AAC→AAT)	pitB	metal phosphate:H(+) symporter PitB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/44);  total (32/44)

GAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GTT‑TGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTC  >  NC_000913/3135320‑3135810                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        gAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGt                                                                                                                                                                                                                                                    <  2:452265/251‑1 (MQ=255) gAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAAAGCGCGCAATAGCATCAAAGGGATTTGCCGGATGACAGTGAAAATCTGTTACTTGCGTGCCATCAGTCGATGCTGCCGGCAATGGAGGTTCCATAGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTTCAGGGAGGGTTTGAAATTGGt                                                                                                                                                                                                                                                    >  2:547852/1‑251 (MQ=255)          gagaTGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGGAACTTTCCATATTGCCTGGCAGCATCGGTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGCGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATc                                                                                                                                                                                                                                           >  1:36950/1‑251 (MQ=255)             aTGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTGAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATcgcg                                                                                                                                                                                                                                        <  2:306574/251‑1 (MQ=255)                     cATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACGTCTGCGGCAGTTCAGGATGCTGTTGCAGGTATTGTTCGAAATTGGTAACGGCATCGcgggtacggg                                                                                                                                                                                                                                <  1:169559/251‑1 (MQ=255)                      aTAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAAATCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGGAGTGTTCGAAATTGGTAACGGGAACGggggtacgggg                                                                                                                                                                                                                               >  1:638215/1‑250 (MQ=255)                        aaTGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGTATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTaa                                                                                                                                                                                                                             <  1:47829/251‑1 (MQ=255)                           gcggcgCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAAtt                                                                                                                                                                                                                           <  1:510699/250‑1 (MQ=255)                               cgcAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATa                                                                                                                                                                                                                      <  1:657309/251‑1 (MQ=255)                                  aGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGcc                                                                                                                                                                                                                   <  1:487459/251‑1 (MQ=255)                                    ctggctgCGCTGACTCACGCTTACCGGCTCGTAACTTTCCACATTTCCTGGCTGCCTCGTTTTAAAGCGCGCAATAGCATCAAAGGGATTTGCCGGATGATAGTGTAAATCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAAATGAGGTTCCCTCGCAATCAACCTTTGCGGCCGTTTAGGGGGGTGGTGCAGGGAGGGTTTGAAATTTGCAACGGCATCAcggggtcggggtattttctatccgg                                                                                                                                                                                                                 >  2:224036/1‑230 (MQ=255)                                      ggctgCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCTGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGGAATTTCATAGCCGGAc                                                                                                                                                                                                               <  1:224036/251‑1 (MQ=255)                                      ggctgCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAATGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGAc                                                                                                                                                                                                               <  1:356828/251‑1 (MQ=255)                                      ggctgCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGa                                                                                                                                                                                                                <  1:194459/250‑1 (MQ=255)                                      ggctgCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGAc                                                                                                                                                                                                               <  1:109428/251‑1 (MQ=255)                                           cgcTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCTATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCAtt                                                                                                                                                                                                          <  1:613194/251‑1 (MQ=255)                                               gACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAAATCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCCGTTCCGGGTGCTGTTGCAGGTAGGGTTTGCAATTTGTAACGGCATCGCGGGGACGGGGAATTTTCTAGCCGGACGCATTcctt                                                                                                                                                                                                      >  1:308085/1‑248 (MQ=255)                                                                 cGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCcagc                                                                                                                                                                                     <  2:638215/250‑1 (MQ=255)                                                                        ttCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGc                                                                                                                                                                               <  1:168568/249‑1 (MQ=255)                                                                                    tGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGt                                                                                                                                                                  >  1:296978/1‑250 (MQ=255)                                                                                     ggCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTa                                                                                                                                                                 >  2:414526/1‑250 (MQ=255)                                                                                       cagcaTCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCCGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGCGGTTCCATCGCAATCAACTTCTGCGGCCGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTgacgac                                                                                                                                                                                              >  1:384435/1‑219 (MQ=255)                                                                                       cagcaTCGTTGTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTGCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGTGTAATTTCATAGCCGGACACATTCATATTgacgac                                                                                                                                                                                              <  2:384435/219‑1 (MQ=255)                                                                                             cGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCAACAGTACCAGCAtt                                                                                                                                                         >  2:162795/1‑250 (MQ=255)                                                                                              gTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTAc                                                                                                                                                       >  1:375879/1‑251 (MQ=255)                                                                                              gTTTTAACGCACGCAATAGCATCAAAGGCATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGCGGCTGTTGCAGGTAGTGTCCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCCATCCCCACCAGTACCAGCATTAc                                                                                                                                                       >  2:237637/1‑251 (MQ=255)                                                                                                  tAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAg                                                                                                                                                    <  1:273744/250‑1 (MQ=255)                                                                                                    aCGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGcc                                                                                                                                                 <  1:78820/251‑1 (MQ=255)                                                                                                     cgcgcgCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCg                                                                                                                                                >  1:164132/1‑251 (MQ=255)                                                                                                          gcAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATccc                                                                                                                                           >  1:547340/1‑251 (MQ=255)                                                                                                           cAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGCGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCt                                                                                                                                          >  1:264441/1‑251 (MQ=255)                                                                                                             aTAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCttt                                                                                                                                        <  2:470175/251‑1 (MQ=255)                                                                                                                  aTCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTc                                                                                                                                   <  2:395982/251‑1 (MQ=255)                                                                                                                  aTCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCAGTACCAGGCCGATCCCTTTTTGTc                                                                                                                                   <  2:423779/251‑1 (MQ=255)                                                                                                                       aGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCcgtcg                                                                                                                              >  2:141304/1‑251 (MQ=255)                                                                                                                               gCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCAGCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGGCCGTCGTTCGCGcc                                                                                                                      <  1:114492/251‑1 (MQ=255)                                                                                                                                 cGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTc                                                                                                                                                                                                                         >  2:461802/1‑150 (MQ=255)                                                                                                                                 cGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTc                                                                                                                                                                                                                         <  1:461802/150‑1 (MQ=255)                                                                                                                                  ggATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTg                                                                                                                   <  1:199516/251‑1 (MQ=255)                                                                                                                                         aGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAac                                                                                                            <  1:609239/251‑1 (MQ=255)                                                                                                                                               cctctGTTTCTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAAGCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAacgccacg                                                                                                      <  2:542846/250‑1 (MQ=255)                                                                                                                                                 tctGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGATGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAacgccacgcc                                                                                                    >  1:626129/1‑251 (MQ=255)                                                                                                                                                    gTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCg                                                                                                  <  2:323583/250‑1 (MQ=255)                                                                                                                                                             gTGCCATCAATCGATGCTGCAGGCAATGGAGGTTCGATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGATCGAAATTGGTAACGGCATCGCGGGGACGGGTAATTTCATAGCCGGACGCATTCCTATTGACGACGAAGCCAGCAGGGGCAATCCCCCCCAGTACCAGCATTACCAGGCAGATCCCTTTTTGTCCGTCGTTAGCGCCCTGCGAAAACCCCACGCCCGCAGCGGCAAc                                                                                        >  2:367205/1‑251 (MQ=255)                                                                                                                                                                         gATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAAt                                                                            <  2:182087/251‑1 (MQ=255)                                                                                                                                                                          aTGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAg                                                                                  >  1:615392/1‑244 (MQ=255)                                                                                                                                                                          aTGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTACGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACTCCCGCAGCGGAAACAATCAg                                                                                  <  2:615392/244‑1 (MQ=255)                                                                                                                                                                                cAGGCAAGGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGGTGCTGTTGAAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATCCGCGTc                                                                     <  2:179276/251‑1 (MQ=255)                                                                                                                                                                                    cAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCTGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGa                                                                 <  2:272117/251‑1 (MQ=255)                                                                                                                                                                                      aTGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAAt                                                               >  1:583309/1‑251 (MQ=255)                                                                                                                                                                                           ggTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCgg                                                          >  1:388953/1‑251 (MQ=255)                                                                                                                                                                                              tCCATAGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGTAACACTCAGCGCAATACGCGTCCAGAATGGCGNttt                                                       >  2:601293/1‑251 (MQ=255)                                                                                                                                                                                                   cGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑Gtt                                                 <  2:160533/251‑1 (MQ=255)                                                                                                                                                                                                      aaTCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATACCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGCTTAccgtttt                                               >  2:86171/1‑245 (MQ=255)                                                                                                                                                                                                         cAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GGT‑TTCCtt                                          >  1:355269/1‑251 (MQ=255)                                                                                                                                                                                                         cAACTTCTGCGGCAGTGCAGGATGCTGTGGCAGGTAGTGTGCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GTT‑TGCCt                                           <  2:486230/250‑1 (MQ=255)                                                                                                                                                                                                          aaCTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GGT‑TTCCttt                                         >  2:116201/1‑251 (MQ=255)                                                                                                                                                                                                             ttCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTTC‑GTT‑TTCCtttttt                                      >  2:418382/1‑251 (MQ=255)                                                                                                                                                                                                                 gCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GTT‑TGCCTTTTTTc                                     <  1:579920/248‑1 (MQ=255)                                                                                                                                                                                                                 gCGGCAGTTCAGGATGCTGTTGCAGGTAGTGGTCGAAATTGGTAACGGTATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTGC‑GTT‑TGCCTTTTTTCttt                                  <  2:272383/251‑1 (MQ=255)                                                                                                                                                                                                                  cGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GTT‑TGCCTTTTTTCtttt                                 <  2:164223/251‑1 (MQ=255)                                                                                                                                                                                                                        ttCAGGATGATGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTAAGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTACTGTT‑T‑CCTTTTTTTTTTTTTCGAt                           >  2:651431/1‑251 (MQ=255)                                                                                                                                                                                                                         tCAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GTT‑TGCCTTTTTTCTTTTTGCGAt                           <  1:601293/250‑1 (MQ=255)                                                                                                                                                                                                                          cAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GTT‑TGCCTTTTTTTTTTTTGCGATCt                         >  1:433236/1‑251 (MQ=255)                                                                                                                                                                                                                          cAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GTT‑TGCCTTTTTTCTTTTTGCGATCt                         <  1:547852/251‑1 (MQ=255)                                                                                                                                                                                                                          cAGGATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GTT‑TGCCTTTTTTCTTTTTGCGATCt                         <  1:116201/251‑1 (MQ=255)                                                                                                                                                                                                                             gATGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GTT‑TGCCTTTTTTCTTTTTGCGATCTTcc                      <  1:137086/251‑1 (MQ=255)                                                                                                                                                                                                                              aTGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGtt                                                                                                                            >  1:206148/1‑150 (MQ=255)                                                                                                                                                                                                                              aTGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGtt                                                                                                                            <  2:206148/150‑1 (MQ=255)                                                                                                                                                                                                                              aTGCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCACGGGTACGGGTAATTTCATAGCAGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCAAACAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTAGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGAGTTC‑GTTATGCCTTTTTT‑TTTTTgtgactttatc                     >  1:46025/1‑241 (MQ=255)                                                                                                                                                                                                                                gCTGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GTT‑TGCCTTTTTTCTTTTTGCGATCTTCCgg                    <  2:164132/250‑1 (MQ=255)                                                                                                                                                                                                                                  tGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAgggg                                                                                                                                                                                <  2:486124/94‑1 (MQ=255)                                                                                                                                                                                                                                  tGTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAgggg                                                                                                                                                                                >  1:486124/1‑94 (MQ=255)                                                                                                                                                                                                                                   gTTGCAGGTAGTGTTCGAAATTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GGT‑TGCCTTTTTTTTTTTTGGGTTCTTCCGGGATg                >  1:561191/1‑251 (MQ=255)                                                                                                                                                                                                                                      gCAGGTCGTGTTCGAAAGTGGTCACGTCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAATCCAGCACGGGCAACTCCCACCAGTCCCAGCATTACCAGGCCGATCCCTTTTTTTCCCTCGTTCGCGCCGTTCGAACACGCCCCGCCCGCAGCGGAAACAATCAGCGTAATACGCGTCCCGAATGGCGGTTTAC‑GTT‑TGCTTTTTTTCTGTTTGCGATCTGCCGGAATGCgg             <  2:253993/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gcGAAATTGGTAACGGAATCGCAGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCATCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGAGCCCGTTTTGTCCGTCGTGCGCGCCGTGCGAAAACGCCGCGCCCGCAGCGGAAACAAGCAGCGCAATACGCGTCCAGAATGGCGTTTTAC‑GTT‑TGCCTTTGTTCTTTTTGCGATCTTCCGGAATGGGGAGAAGACGGTc  <  2:623772/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        GAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAAGGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCGCGGGTACGGGTAATTTCATAGCCGGACGCATTCATATTGACGACGAAGCCAGCAGGGGCAATCCCCACCAGTACCAGCATTACCAGGCCGATCCCTTTTTGTCCGTCGTTCGCGCCGTGCGAAAACGCCACGCCCGCAGCGGAAACAATCAGCGCAATACGCGTCCAGAATGGCGGTTTAC‑GTT‑TGCCTTTTTTCTTTTTGCGATCTTCCGGAATGCGGTGAATACGGTC  >  NC_000913/3135320‑3135810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑