Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	896,433	C→T	L100L (CTC→CTT)	potH →	putrescine ABC transporter membrane subunit PotH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	896,433	0	C	T	100.0%	231.9 / NA	69	L100L (CTC→CTT)	potH	putrescine ABC transporter membrane subunit PotH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/39);  total (30/39)

TGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTG‑GTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTCCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCT  >  NC_000913/896188‑896675                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   gggTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTG‑GTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCGATTCCACCTGATACCGAACTGATGGAGTGGGCTGACGGGTAACTGTCCATCTCTCTTAATCTCGGTAATTTTCGGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTc                                                                                                                                                                                                                                               <  2:365686/250‑1 (MQ=255)  ggTTGTCGCAGCGACAAGTGCGGCATGGGCGCAAATTGCGGC‑TTGCGTTGCCTTATAACTGGTTGATCTTGCTGTTACTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGTTGACGTGCAACTGTCCGTAACTATTAATCTCGATAATTCTCTGCAACTGACAGACGATACGCTATATTTCGATGCTTATCTTCAGTCg                                                                                                                                                                                                                                              >  2:633760/1‑251 (MQ=255)   gTTGTAGCAGCTGCAAATGAAGCATGGGCGCAAACTG‑GTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGc                                                                                                                                                                                                                                             >  2:554877/1‑251 (MQ=255)     tGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTG‑GTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCt                                                                                                                                                                                                                                            <  2:91629/250‑1 (MQ=255)         gCAGCTGAAAATGAAGCATGGGCGCAAACTG‑GTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCGATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCGTAATCTCGGTAATTTTCTGCAACTGACCGACGATCAGCTCTATTTCGATGCTTACCTTCCGTCGCAACAgg                                                                                                                                                                                                                                       >  2:264206/1‑251 (MQ=255)          cAGCTGCAAATGAAGCATGGGCGAAAACTG‑GTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGt                                                                                                                                                                                                                                      >  2:36586/1‑251 (MQ=255)               gCAAATGAAGCATGGGCGCAAACTG‑GTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCt                                                                                                                                                                                                                                  <  2:137748/250‑1 (MQ=255)               gCAAATGAAGCATGGGCGCAAACTG‑GTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGAGTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCGCTATTTCGATGCTTAGCTTCAGTCGCTACAGGTGGc                                                                                                                                                                                                                                   <  1:532055/249‑1 (MQ=255)                cAAATGAAGCATGGGCGCAAACTG‑GTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGc                                                                                                                                                                                                                                >  1:448210/1‑251 (MQ=255)                   aTGAAGCATGGGCGCAAACTG‑GTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCAGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGAt                                                                                                                                                                                                                             <  1:173742/251‑1 (MQ=255)                     gAAGCATGGGCGCAAACTG‑GTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGAttt                                                                                                                                                                                                                           >  2:249641/1‑251 (MQ=255)                     gAAGCATGGGCGCAAACTG‑GTCATTGCGTTGCCATAAATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATTCTTATCTTCAGTCGCTACAGGTGGCTGCGAtt                                                                                                                                                                                                                            <  1:30781/250‑1 (MQ=255)                                  aaaCTG‑GTCAGTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCAGTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACGGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTTACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTg                                                                                                                                                                                                              <  2:644422/251‑1 (MQ=255)                                    aCTG‑GTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGtt                                                                                                                                                                                                            <  1:528470/251‑1 (MQ=255)                                          tCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTg                                                                                                                                                                                                       >  2:540747/1‑251 (MQ=255)                                          tCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTg                                                                                                                                                                                                       >  2:350352/1‑251 (MQ=255)                                           cATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTg                                                                                                                                                                                                       >  1:174144/1‑250 (MQ=255)                                            aTTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGTCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCATTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGAt                                                                                                                                                                                                     <  1:611790/251‑1 (MQ=255)                                              tGCGTTGCCATATATCTGGTTGATCTTGCTTTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCTCCTTATACCGAACTGATGGATTTGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATc                                                                                                                                                                                                    <  1:15356/250‑1 (MQ=255)                                               gCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCgg                                                                                                                                                                                                  <  1:350352/251‑1 (MQ=255)                                               gCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCg                                                                                                                                                                                                   <  1:197111/250‑1 (MQ=255)                                                   tGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTa                                                                                                                                                                                               <  2:508481/250‑1 (MQ=255)                                                     ccATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCGGTTTACTGATCGGCTATcc                                                                                                                                                                                            <  1:249641/251‑1 (MQ=255)                                                       atatatCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTCAAATAAGACTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGCTGGAGTGGGCTGACGGGCAACTCTACATCAATCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTCCTGATCGGCTATCCGc                                                                                                                                                                                          >  1:409148/1‑251 (MQ=255)                                                                        tGCTGTTTCTGCTGCCATGTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTgg                                                                                                                                                                           <  1:102155/249‑1 (MQ=255)                                                                          cTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGAc                                                                                                                                                                                                                       >  2:581152/1‑203 (MQ=255)                                                                          cTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGAc                                                                                                                                                                                                                       <  1:581152/203‑1 (MQ=255)                                                                            gTNTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTTGCGGAGATGGCGCGCGCTATTCCTCCGTATACCGAACTGATAGAGTGGGCTGACGGGCAACTGTCCATCTCGCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGGTCTATTTCGATGTTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGTCATTTTGCTGTTTACTGATCGGCTATCCGCTGGCTTTGGCGGTGGCGcac                                                                                                                                                                      <  2:604466/250‑1 (MQ=255)                                                                              ttCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAg                                                                                                                                                                    <  1:435844/250‑1 (MQ=255)                                                                                    tgcCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCt                                                                                                                                                             >  1:445302/1‑251 (MQ=255)                                                                                    tgcCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCt                                                                                                                                                             >  1:202950/1‑251 (MQ=255)                                                                                       cATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCg                                                                                                                                                          <  1:214101/251‑1 (MQ=255)                                                                                               aTTGTCTTTAAAATAAGCCTGGCGGAGATTGAGAGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGTTATCCGCTGGCGCGGGCGGTGGCGCACAGTAAGATTTCGACCCGTa                                                                                                                                                   >  1:632138/1‑250 (MQ=255)                                                                                                           aTAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATCCCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATtact                                                                                                                                      <  2:445302/251‑1 (MQ=255)                                                                                                             aaGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATtacta                                                                                                                                     <  1:397094/250‑1 (MQ=255)                                                                                                                ccTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTgg                                                                                                                                 >  2:106847/1‑251 (MQ=255)                                                                                                                ccTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTAGACCCGTAATATTTTATTACTACTgg                                                                                                                                 >  2:515356/1‑251 (MQ=255)                                                                                                                  tGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGa                                                                                                                                                         <  1:106426/225‑1 (MQ=255)                                                                                                                  tGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGa                                                                                                                                                         >  2:106426/1‑225 (MQ=255)                                                                                                                   ggCGGAGATGGCGCGCGCTATTCCTCCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTGTCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGa                                                                                                                              <  2:204667/251‑1 (MQ=255)                                                                                                                         gaTGGCGCGAACTATTCCCCACTAGACCGAACGGGTGGAGTGTGGTTACGGGCAACTGTCCGTCTCTCTTAATCTCGTTAATTTTCTGCTACTGCCCGACGAACCGCTCTATTCCGATGCGTATCTTCAGTCGCTACAGGTGGCTGCTATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGTGGTTGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGTGGATCCTGc                                                                                                                        <  2:634158/251‑1 (MQ=255)                                                                                                                               gcgcgcTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCcgtcgt                                                                                                                  >  1:383079/1‑251 (MQ=255)                                                                                                                                cgcgcTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAACCCTTCGACCCGTAATATTTTCTTACTACTGGTGATCCTGCcgtcttg                                                                                                                 >  2:346421/1‑248 (MQ=255)                                                                                                                                   gatATTCCACCTTAAACCGAACTGATGGAGTGGGCTGACGTGCAACTGTCCATCACTCTTAAGCTCGGTAATTTGCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGTCGGTGGCGCACAGTAAGCCTGCGACCCGTAAGATTTTATTGCTACTTGTGATGCTGCCGTCGTGGAc                                                                                                              <  2:396670/249‑1 (MQ=255)                                                                                                                                       ttCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCg                                                                                                          >  1:357579/1‑251 (MQ=255)                                                                                                                                       ttCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCg                                                                                                          >  2:504994/1‑251 (MQ=255)                                                                                                                                               tataCCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGAt                                                                                                  >  1:43482/1‑251 (MQ=255)                                                                                                                                                  aCCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATcc                                                                                                <  1:504994/250‑1 (MQ=255)                                                                                                                                                       aCTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGTTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCGGGTTTCTGGTCCGCGttt                                                                                          <  2:627760/251‑1 (MQ=255)                                                                                                                                                           aTGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGc                                                                                      <  1:554877/251‑1 (MQ=255)                                                                                                                                                                gTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGa                                                                                 <  1:264206/251‑1 (MQ=255)                                                                                                                                                                  gggCTGACGGGCATCTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACTATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCTTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATg                                                                               <  2:506100/251‑1 (MQ=255)                                                                                                                                                                   ggCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCTGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATgg                                                                              <  1:26274/251‑1 (MQ=255)                                                                                                                                                                    gCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATggg                                                                             <  1:484108/251‑1 (MQ=255)                                                                                                                                                                       gACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGa                                                                            <  2:232101/249‑1 (MQ=255)                                                                                                                                                                                       tCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGgt                                                          >  1:183007/1‑251 (MQ=255)                                                                                                                                                                                                 tctcGGGACTTTTCTGCAACTGACCGTCCATCCGCTCTATTTCGCTGCTTATCTTCAGTCGCTACAGGCGGCTGCGATTTCGACATTTTGCTGTTTACTGCTCGCCTATCCGCTGGCGTTGGCGGTGGCGCACAGTCAGCCTTCGACCCGTAATATTTTATTACTACTGTTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGaat                                                 >  2:150999/1‑250 (MQ=255)                                                                                                                                                                                                    cGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGaata                                                <  1:183989/248‑1 (MQ=255)                                                                                                                                                                                                          ttttCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCACTCCAGGAGGCTGCGCTTTCGCCATTTTGCTGTTTACTGATCGGCTATCCGCTGACGTGGGCGGTGGCGCACAGTAAGACTTCGACCCGTAATCTTTTATTACTACTGGCGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTctgc                                       >  1:88525/1‑251 (MQ=255)                                                                                                                                                                                                            ttCTGCAACTGACCGGCGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCGATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTctgct                                      <  1:159056/250‑1 (MQ=255)                                                                                                                                                                                                                gCAACTGACCGACGGTCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACATGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGTTGGCGTACAGTAAGCCTTCGACCCGTAAGATTTTATTACTACTGGTGAGCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGGGCTGAATAATTTTCTGCGGTGGc                                 <  2:375464/251‑1 (MQ=255)                                                                                                                                                                                                                gCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGc                                 >  2:323319/1‑251 (MQ=255)                                                                                                                                                                                                                     tGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTgggg                            >  1:578129/1‑251 (MQ=255)                                                                                                                                                                                                                     tGACCGACGATCCGCTCTATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTA‑TATTTTATTACTACTGGTGATCCTTCCGTCGTGGCCCTCGTTTTTTATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAAAGGTGTGGCGGATTATTTTCTGCTGTGGCgggggg                           >  1:8606/1‑251 (MQ=255)                                                                                                                                                                                                                             gaTCCGCTCTATTTCGATCCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTACGCCTTCGACCCGTAATCTTTTATTACTACTGGTTATCCTGCCGTCGTGGACATCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCg                     >  2:260354/1‑250 (MQ=255)                                                                                                                                                                                                                                  gCTCAATTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGAGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGTTCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGCATATTAAAAAACACCGGTGTGCTGATTACTTTTCTGCTTTGGCTGGGGGTTATCGATCAAc               >  2:167985/1‑251 (MQ=255)                                                                                                                                                                                                                                       aTTTCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCt           <  1:540747/250‑1 (MQ=255)                                                                                                                                                                                                                                         ttCGATGCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGa         <  2:174144/250‑1 (MQ=255)                                                                                                                                                                                                                                               gCTTATCTTCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCt  <  1:106847/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   TGGTTGTCGCAGCTGCAAATGAAGCATGGGCGCAAACTG‑GTCATTGCGTTGCCATATATCTGGTTGATCTTGCTGTTTCTGCTGCCATTTCTGATTGTCTTTAAAATAAGCCTGGCGGAGATGGCGCGCGCTATTCCACCTTATACCGAACTGATGGAGTGGGCTGACGGGCAACTGTCCATCACTCTTAATCTCGGTAATTTTCTGCAACTGACCGACGATCCGCTCTATTTCGATGCTTATCTCCAGTCGCTACAGGTGGCTGCGATTTCGACATTTTGCTGTTTACTGATCGGCTATCCGCTGGCGTGGGCGGTGGCGCACAGTAAGCCTTCGACCCGTAATATTTTATTACTACTGGTGATCCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCT  >  NC_000913/896188‑896675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑