Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,057,826	C→T	Q43Q (CAG→CAA)	torR ←	DNA‑binding transcriptional dual regulator TorR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,057,826	0	C	T	100.0%	196.7 / NA	59	Q43Q (CAG→CAA)	torR	DNA‑binding transcriptional dual regulator TorR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/30);  total (29/30)

GTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTCTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTGTTCATATTTGCTCATTAAGATCGCTTCACTAAACCATAATTC  >  NC_000913/1057582‑1058070                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     gTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGTAATCGTCTGCGCTCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATa                                                                                                                                                                                                                                                <  1:224079/251‑1 (MQ=255)         aGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCg                                                                                                                                                                                                                                        >  1:436603/1‑251 (MQ=255)          ggTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGt                                                                                                                                                                                                                                       <  1:599796/251‑1 (MQ=255)            tCGATTCGCCAGTGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGAGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATACGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGCTTTTGCATAATTTCCCgc                                                                                                                                                                                                                                       >  2:360561/1‑248 (MQ=255)             cGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGc                                                                                                                                                                                                                                    >  1:98028/1‑251 (MQ=255)              gATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGcc                                                                                                                                                                                                                                   <  1:302435/251‑1 (MQ=255)                    ccAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCAc                                                                                                                                                                                                                             >  1:335242/1‑251 (MQ=255)                     cAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGGAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCAcc                                                                                                                                                                                                                            <  2:46064/251‑1 (MQ=255)                                      ccGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGaaa                                                                                                                                                                                                           <  1:570484/251‑1 (MQ=255)                                        gTACTACCAGTTCGAGCAGTTCCAGCGGTTTGGTGACGTACTCCTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTCCCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCAGTAACGGAAACg                                                                                                                                                                                                         >  2:386836/1‑251 (MQ=255)                                                                  ggTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATCCGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGa                                                                                                                                                                               <  2:497263/251‑1 (MQ=255)                                                                    tttGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGt                                                                                                                                                                                                                                       <  1:32310/193‑1 (MQ=255)                                                                    tttGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGt                                                                                                                                                                                                                                       >  2:32310/1‑193 (MQ=255)                                                                                       gcgcCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTa                                                                                                                                                            <  1:233525/249‑1 (MQ=255)                                                                                       gcgcCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCCCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTa                                                                                                                                                            <  2:395110/249‑1 (MQ=255)                                                                                        cgcCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAAcc                                                                                                                                                         <  1:397035/251‑1 (MQ=255)                                                                                        cgcCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAAcc                                                                                                                                                         <  1:577597/251‑1 (MQ=255)                                                                                              tttccaggaaaaccaTACGGCCAATGCGATCGCTGCGTTCGGTAACCATAATAATCCCCACCGTTGAGCGTTCTCGCAGGGAACGGCTTACCATAAGGCCATTTTGATCGGGTAAGTTGATATCCAGCAGAATTAAATATAGCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGCATACCCCTCCTGAGTGAAGTAGGATTGTAATCGTGCCTGGGTAACCGGCTct                                                                                                                                                   >  2:283595/15‑250 (MQ=255)                                                                                                  cAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCCCCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAAACGGCTCATCtt                                                                                                                                               >  1:390202/1‑251 (MQ=255)                                                                                                    gCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCACCACCGGTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGAAACCGGCTCATCTTc                                                                                                                                              >  1:82776/1‑250 (MQ=255)                                                                                                            aTACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCTGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCaacaataac                                                                                                                                     <  1:285766/251‑1 (MQ=255)                                                                                                              aCGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATGCCAACCGTTGCGCGTTCTCGCAGGGCGCGGGGTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACACCTCCTGAGCGACGTAGGATTGTAATCGCGCCTGGGTCACCGGCTCATCTTCaacaataaca                                                                                                                                    >  2:268805/1‑250 (MQ=255)                                                                                                                 gTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCTGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAatgt                                                                                                                                <  1:374872/251‑1 (MQ=255)                                                                                                                    aaTCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAatgtgat                                                                                                                             >  1:536759/1‑251 (MQ=255)                                                                                                                        cGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATCAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAatgtgatgggg                                                                                                                         >  2:643112/1‑248 (MQ=255)                                                                                                                                 cGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAgg                                                                                                                <  2:113026/251‑1 (MQ=255)                                                                                                                                   tCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACCTAACGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGt                                                                                                              >  1:424476/1‑251 (MQ=255)                                                                                                                                    ccGGTAACCAGAATTATCCCCGCCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGGAACGGAAGCGTTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGtt                                                                                                             <  1:56703/251‑1 (MQ=255)                                                                                                                                     cggaacccagactaatcacaacacttGAGCGTTCTCGCCGGAAACGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATCTCCAGCAGAATTAACTCTATCGACTGATTTTGCATACTTTCCCGTAGACCAGAACCGCTCGCCGTAACGGAAACGGTATACCACTCCTGAGTGACGCAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGCGATGCGGCATAAGAGGGttt                                                                                                            >  2:425277/25‑251 (MQ=255)                                                                                                                                      ggTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGtttt                                                                                                           >  2:159808/1‑251 (MQ=255)                                                                                                                                       gTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCTCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTa                                                                                                          <  1:606241/251‑1 (MQ=255)                                                                                                                                               aataatCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATtct                                                                                                  <  2:247158/251‑1 (MQ=255)                                                                                                                                                           cGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATtctgttcatatctgt                                                                                      <  1:79969/251‑1 (MQ=255)                                                                                                                                                            gTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGACGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATtctgttcatatctgtt                                                                                     >  2:182973/1‑251 (MQ=255)                                                                                                                                                            gTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATtctgttaatatctgtt                                                                                     >  1:131491/1‑251 (MQ=255)                                                                                                                                                               gAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATtctgttcatatctgttcat                                                                                  <  1:355962/251‑1 (MQ=255)                                                                                                                                                                  cGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATAtt                                                                               >  1:92642/1‑251 (MQ=255)                                                                                                                                                                  cGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATAtt                                                                               >  2:630777/1‑251 (MQ=255)                                                                                                                                                                     tctcGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATGTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATGTCTGTTCATATTCt                                                                             <  2:613736/250‑1 (MQ=255)                                                                                                                                                                     tctcGCAGGGAGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAGTGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATGGCGACTGGGTAACCGGCTCAGCTTCAACAATAACGATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATAGCTGGTCCTATTCt                                                                             <  2:76860/250‑1 (MQ=255)                                                                                                                                                                         gCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGt                                                                        >  1:199507/1‑251 (MQ=255)                                                                                                                                                                           aGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCgcgc                                                                                                                                                                   >  2:120775/1‑158 (MQ=255)                                                                                                                                                                           aGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCgcgc                                                                                                                                                                   <  1:120775/158‑1 (MQ=255)                                                                                                                                                                            gggCGCGGGTTAACATCAGTCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCGACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTTGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAg                                                                     <  1:93543/251‑1 (MQ=255)                                                                                                                                                                               cgcgGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCt                                                                                                                                                                 >  2:146139/1‑156 (MQ=255)                                                                                                                                                                               cgcgGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAAGTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCt                                                                                                                                                                 <  1:146139/156‑1 (MQ=255)                                                                                                                                                                                                     ttCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTg                                            >  1:28174/1‑251 (MQ=255)                                                                                                                                                                                                      tCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTg                                            <  1:55706/250‑1 (MQ=255)                                                                                                                                                                                                       cATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTGtt                                          >  1:280761/1‑251 (MQ=255)                                                                                                                                                                                                                    gATATCCAGCTGAATTAAATCTACCGACTGATGTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGGATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTGTTCATATTTGCTCa                              <  1:295790/250‑1 (MQ=255)                                                                                                                                                                                                                            gcagAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTGTTCATATTTGCTCATTAAGATc                      >  1:284044/1‑250 (MQ=255)                                                                                                                                                                                                                            gcagAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTGTTCATATTTGCTCATTAAGATc                      <  2:284044/250‑1 (MQ=255)                                                                                                                                                                                                                            gcagAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTGTTCATATTTGCTCATTAAGATCg                     <  2:335242/251‑1 (MQ=255)                                                                                                                                                                                                                              agAATTAAATCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTGTTCATATTTGCTCATTAAGATCGCt                   >  2:49128/1‑251 (MQ=255)                                                                                                                                                                                                                                 agtAAATCTACCGACTGAGTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCAACCTGACCAGTGCCGCTGTTCATATTTGCTCATTAAGATCGCTTCa                <  1:630777/249‑1 (MQ=255)                                                                                                                                                                                                                                     aaTCTACCGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTGTTCATATTTGCTCATTAAGATCGCTTCACTa             >  1:444725/1‑250 (MQ=255)                                                                                                                                                                                                                                      aTCTAACGACTGATTTTGCATACTTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGCAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTGTTCATATTTGCTCATTAAGATCGCTTCACTaa            >  2:315539/1‑250 (MQ=255)                                                                                                                                                                                                                                           ccGACTGATTTTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGGTTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTGTTCATATTTGCTCATTAAGATCGCTTCACTAAACCAt       >  2:21455/1‑250 (MQ=255)                                                                                                                                                                                                                                                tGATTTTGCATAATTGCCCTGAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTGTTCATATTTGCTCATTAAGATCGCTTCACTAAACCATAATTc  <  2:611034/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     GTCGCGCGAGGTCGATTCGCCAGAGCAGATTTTTCACCCGTACTACCAGTTCGCGCAGTTCCAGCGGTTTGGTGACGTAATCGTCTGCGCCCATTTCCAGCCCAACAATACGGTCAATCCGATCGCTGCGTCCGGTAACCAGAATAATCCCCACCGTTGAGCGTTCTCGCAGGGCGCGGGTTAACATCAGGCCATTTTCATCGGGTAAGTTGATATCCAGCAGAATTAAATCTACCGACTGATTCTGCATAATTTCCCGTAGCCCAGCACCGCTCGCCGTAACGGAAACGGTATACCCCTCCTGAGTGAAGTAGGATTGTAATCGCGCCTGGGTAACCGGCTCATCTTCAACAATAACAATGTGATGTGGCATCAGAGGGTTTTACTCATTCTGTTCATATCTGTTCATATTCTGCCGTAAGCCGTTCATCCTGACCAGTGCCGCTGTTCATATTTGCTCATTAAGATCGCTTCACTAAACCATAATTC  >  NC_000913/1057582‑1058070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑