Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,045,508	C→T	G132S (GGC→AGC)	ygfF ←	putative oxidoreductase YgfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,045,508	0	C	T	100.0%	206.3 / NA	63	G132S (GGC→AGC)	ygfF	putative oxidoreductase YgfF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/36);  total (27/36)

GGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAACATCGCAACGACCTGGTTTTCGTCGCTGATATCCGCCTGGAGCACGAATGCTTTGCCA  >  NC_000913/3045270‑3045752                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     gggATGTTCGACATAACTCGATCGACGCTTCCAGGCTCTCCGCCGCTGGCGTGCATTGCGGGATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCTACTTCCAGCTATAGTCCGGTGGTTAACGTATCAATCGCCCCTTGCGATGCCGCGTAATCAACATATTCCCCGGGCGAACCCAACCGGGAGGCCACCGAAGAGACATTGACGATAGCGCCGCGACTGGTACCATATTTAAg                                                                                                                                                                                                                                          <  2:616381/251‑1 (MQ=255)                       cgccgcgTCCAGGCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTc                                                                                                                                                                                                                     <  1:342150/247‑1 (MQ=255)                        gACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTcgc                                                                                                                                                                                                                   >  2:631189/1‑251 (MQ=255)                          cgcgCCCAGGCTCGCCTCCGCTGGCGTCCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGCCTGCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCACCCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCgg                                                                                                                                                                                                                 <  2:365722/251‑1 (MQ=255)                                      cgccgccgcTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGaaa                                                                                                                                                                                                      >  1:136577/1‑250 (MQ=255)                                          gccgcTGGCGTCCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTACCGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTACCGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGTTAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATc                                                                                                                                                                                                 <  1:147389/251‑1 (MQ=255)                                           ccgcTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGCCTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATcc                                                                                                                                                                                                <  1:574622/251‑1 (MQ=255)                                                 gCGTGCATTGCGGTATAAATAATCCCTGGCCGCCCGCAGTTACCGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCAc                                                                                                                                                                                          <  1:360744/251‑1 (MQ=255)                                                  cGTGCATTTCGGTATAAATAACCCCTGGCAGCACGCAGTTACCGCGGATCCCCTGCCCGGCGCCTTCCAGCGATATTCCGGTGTTTAACGTATCAATCGCCCCTTCCGATGCCGCGTAATCAACATATTCCCCTGGCGACCCCACCCGTGAGGCCACCGAAGAGACATTGACGATTTCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCCCGGCAGCTGAGAATATATCCCGTCACg                                                                                                                                                                                         <  2:276100/251‑1 (MQ=255)                                                         ttCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCATTCGCCCCTTTCGATGCCGCGTACTCAACATATTCCCCTGGCGAACCCAACCGCGAGGCCACCGAAGAGTCCTTGACGATAGCGCCGCCACTGCTACCATTTTTCATCGCCATGCGTTTTACCGCCTCGCGGCCGCAGAGATAATATCCCGTCACGTtggtgg                                                                                                                                                                                  >  2:503269/1‑251 (MQ=255)                                                          tCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGCCTTCCAGCTATAGTCCGGTTGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCACCATATTCCCCTGGCAAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTtggtgg                                                                                                                                                                                  <  1:151430/250‑1 (MQ=255)                                                                   ataaaCCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGACAGTACTCg                                                                                                                                                                        >  2:246630/1‑251 (MQ=255)                                                                      aaCNCTGGCCGCGCGCAGTTAACGCGGATCCCCGGCGCGGCGACTTCCAGCGATAGTCCGGTGGGTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCTTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGtt                                                                                                                                                                     <  2:591101/251‑1 (MQ=255)                                                                       aCCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGCGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGACAGTACTCGGTTg                                                                                                                                                                    >  2:138580/1‑251 (MQ=255)                                                                         ccTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGAt                                                                                                                                                                  <  1:651662/251‑1 (MQ=255)                                                                                   cgcaGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCg                                                                                                                                                        <  2:33780/251‑1 (MQ=255)                                                                                              cgGAACCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAAAATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTAACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTc                                                                                                                                             >  2:661578/1‑251 (MQ=255)                                                                                               ggATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTc                                                                                                                                             >  2:126421/1‑250 (MQ=255)                                                                                                 aTCCCCTGGTCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAAc                                                                                                                                          <  2:533511/251‑1 (MQ=255)                                                                                                  tccctggctcggcgACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATAAACATATTCCCCTGGCGAACCCACCCGTGAGGCCACCAAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAAc                                                                                                                                          <  2:422166/241‑1 (MQ=255)                                                                                                  tCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCt                                                                                                                                                                                                                      <  2:35398/174‑1 (MQ=255)                                                                                                  tCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCt                                                                                                                                                                                                                      >  1:35398/1‑174 (MQ=255)                                                                                                   ccccTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACgg                                                                                                                                        >  1:385720/1‑251 (MQ=255)                                                                                                    cccTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGACCCCAACCGTGAGGCCCCCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTCCTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGt                                                                                                                                       <  1:418651/251‑1 (MQ=255)                                                                                                    cccTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGt                                                                                                                                       >  1:544939/1‑251 (MQ=255)                                                                                                       ggcgcGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCTCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCa                                                                                                                                    <  2:362871/250‑1 (MQ=255)                                                                                                          gcggcgACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTCCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCCGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTg                                                                                                                                 >  2:461813/1‑251 (MQ=255)                                                                                                            ggctaCTTCCAGCGATAGTCCGGTGGTTAACGTACCAATCGCCCCTTTCGATGCCGCGTAATCAACATATCCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTggg                                                                                                                               <  2:470625/247‑1 (MQ=255)                                                                                                                   tCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAAc                                                                                                                          <  1:526694/249‑1 (MQ=255)                                                                                                                   gccAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACaa                                                                                                                        <  2:126909/250‑1 (MQ=255)                                                                                                                        cGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTaa                                                                                                                                                                                                                                           >  1:13121/1‑131 (MQ=255)                                                                                                                        cGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTaa                                                                                                                                                                                                                                           <  2:13121/131‑1 (MQ=255)                                                                                                                          aTAGTCCGGGGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTGTTCAACGGTGCACTGGGTAAACAAGATCCCg                                                                                                                 <  1:582812/251‑1 (MQ=255)                                                                                                                                cGGTGGTTAACGTATCAATCGCCCATTTCGATGCCGCGTAATCAACATATTCCACTGGCGAACCCAACCGCGAGGCCACCGCAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTACGGTTTTCAACGGTGAACTGGGTACACAAGATCCCGGCGtt                                                                                                            >  2:445083/1‑250 (MQ=255)                                                                                                                                       tAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCAACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGAcc                                                                                                     >  1:116779/1‑250 (MQ=255)                                                                                                                                         aCGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGTCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCTGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCTCTCTGCGGTAAGGTGTTCAACGGTGCACTGGGGAAACAAGATCCCGGCGTTATTGACCAgc                                                                                                  >  1:366523/1‑251 (MQ=255)                                                                                                                                            tATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCgct                                                                                               >  1:195474/1‑251 (MQ=255)                                                                                                                                               cAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGACCCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCgctgct                                                                                            <  2:181817/251‑1 (MQ=255)                                                                                                                                                  tCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGATAGTACTCGGTTGATTCTCTCTGCGGTAAGGTTTTCACCGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGc                                                                                         >  2:178501/1‑251 (MQ=255)                                                                                                                                                   cGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCg                                                                                        >  1:332474/1‑251 (MQ=255)                                                                                                                                                   cGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGCACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCg                                                                                        >  1:434304/1‑251 (MQ=255)                                                                                                                                                   agccacTTTCGAGCCCGCTTAAACAACAACTTCCCCTGTCGAACCCAACCGTGAGGCCACCGAAGAGACATTGCCGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTGACCGCCGCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGGTTTCAGCGGTGCACCGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCg                                                                                        <  2:639628/246‑1 (MQ=255)                                                                                                                                                    gCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCgg                                                                                       <  1:22904/251‑1 (MQ=255)                                                                                                                                                     ccccTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGt                                                                                      >  2:534474/1‑251 (MQ=255)                                                                                                                                                      cccTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGtt                                                                                     <  1:126421/251‑1 (MQ=255)                                                                                                                                                       ccTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTc                                                                                    <  1:631189/251‑1 (MQ=255)                                                                                                                                                          tccGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCa                                                                                   <  1:35233/247‑1 (MQ=255)                                                                                                                                                            cGATGCCGCGAAATCAACATATTCCCCTGTCGAACCCAACCGTGAGGCCAGCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGCGAAAATATCCCGTCACGTTGGTGGCAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGAGCCCGGCGGTATTGACCAGCGCTGCTAGCGGTTCATcat                                                                               >  2:348282/1‑249 (MQ=255)                                                                                                                                                             gATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTg                                                                              <  2:198207/251‑1 (MQ=255)                                                                                                                                                               tGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCt                                                                            >  1:309951/1‑251 (MQ=255)                                                                                                                                                                          cAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCt                                                                 >  1:592435/1‑251 (MQ=255)                                                                                                                                                                                atTCCCCGGGCGAACCCAACCGTGAGGCCCCCGAGGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTaaa                                                            <  2:112203/250‑1 (MQ=255)                                                                                                                                                                                           aaacccagccggtaGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTGACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGAGTCGCGCTGCGGTAAGGTTTGCAACGGTGGACGGGGTAAACAAGATCCCTGCGTTATTGACCAGCGCTGCGAGCGGTTCATCGTGCTGATCGATTGCGGTAAACATCGGAACGAc                                                <  2:629440/238‑1 (MQ=255)                                                                                                                                                                                             aCCCAACCGTGAGGCCACCGAGGAGACATGGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCGCGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAACATCGCAACGACCt                                              <  2:500397/251‑1 (MQ=255)                                                                                                                                                                                             aCCCAACCGTGAGGCCACCGAAGAGAGATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGAAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGAGCAGCGCTGCTAGCGGTTCATCGTTCTGAACGATTGCTGTAAACATCGCAACGACCt                                              <  1:108323/251‑1 (MQ=255)                                                                                                                                                                                                 aaCCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCAGTCACGTTGGTGAAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAACATCGCAACGACCTGGtt                                          >  1:254912/1‑251 (MQ=255)                                                                                                                                                                                                       gAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAACATCGCAACGACCTGGTTTtcgtc                                    <  1:71755/251‑1 (MQ=255)                                                                                                                                                                                                       gAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTACTAGCGGTTCATCGTGCTGATCGATTGCTGTAAACATCGCAACGACCTGGTTTtcgtc                                    >  1:620472/1‑251 (MQ=255)                                                                                                                                                                                                         ggCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAACATCGCAACGACCTGGTTTTCGTCGc                                  >  2:22714/1‑251 (MQ=255)                                                                                                                                                                                                                   gagaCATTGACGATAGCGCCGCCACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTTCTGTAAACATCGCAACGACCTGGTTTTCGTCGCTGATATCCGc                        <  1:558681/251‑1 (MQ=255)                                                                                                                                                                                                                                      cgccACTGCTACCATTTTTGAGCGCCATGCGTTTTACCGCCGCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGAGTCGCTCTGCGGTAAGGTTTTCAACGGTGCCCTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAACATCGCAACGACCTGGTTTTCGTCGCTGATATCCGCCTGGAGCACGAATGCTTTg     <  1:323474/251‑1 (MQ=255)                                                                                                                                                                                                                                         cACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAACATCGCAACGACCTGGTTTTCGTCGCTGATATCCGCCTGGAGCACGAATGCTTTGcc   >  2:18799/1‑250 (MQ=255)                                                                                                                                                                                                                                         cACTGCTACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAACATCGCAACGACCTGGTTTTCGTCGCTGATATCCGCCTGGAGCACGAATGCTTTGCCa  <  2:45732/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     GGGATGTTCGACTTAACGCGATCGACGCGTCCAGGCTCGCCGCCGCTGGCGTGCATTTCGGTATAAATAAACCCTGGCCGCACGCAGTTAACGCGGATCCCCTGCGCGGCGACTTCCAGCGATAGTCCGGTGGTTAACGTATCAATCGCCCCTTTCGATGCCGCGTAATCAACATATTCCCCTGGCGAACCCAACCGTGAGGCCACCGAAGAGACATTGACGATAGCGCCGCCACTGCCACCATTTTTAAGCGCCATGCGTTTTACCGCCTCGCGGCAGCAGAGAAAATATCCCGTCACGTTGGTGGAAAGTACTCGGTTGATTCGCTCTGCGGTAAGGTTTTCAACGGTGCACTGGGTAAACAAGATCCCGGCGTTATTGACCAGCGCTGCTAGCGGTTCATCGTGCTGATCGATTGCTGTAAACATCGCAACGACCTGGTTTTCGTCGCTGATATCCGCCTGGAGCACGAATGCTTTGCCA  >  NC_000913/3045270‑3045752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑