Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,634	C→T	S325N (AGC→AAC)	pyrC ←	dihydroorotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,634	0	C	T	97.4%	119.9 / ‑3.6	39	S325N (AGC→AAC)	pyrC	dihydroorotase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (21/16);  total (21/18)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

GAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTA  >  NC_000913/1121400‑1121879                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      gAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGtt                                                                                                                                                                                                                                        <  1:468678/250‑1 (MQ=255)        gcggGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCa                                                                                                                                                                                                                                <  1:123019/251‑1 (MQ=255)          gggAAAGGTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGACGTTTTCGCTATGGTAACTTCAATTCACATAATAGCCCCCTGTTGAGTATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCGACGATTTGTATTGTTTAAC‑GGACCAGCGTTCCGTTTCCCGGGCGAGGAATGGCTCCAGCGTGTCAGCAGCCAGTGCGCCGTTTTCAGCAAccagcttcgcttcacg                                                                                                                                                                                                                              <  2:260686/251‑15 (MQ=255)            gAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGt                                                                                                                                                                                                                             >  2:432192/1‑250 (MQ=255)                           agGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAAtg                                                                                                                                                                                                             >  1:155643/1‑251 (MQ=255)                                gtaaatagcacagggtGGCAATGGAAAAGTTTACACTAAGGTGACTACAATTGGGATAATAGCCCCCTGTTGAATAAACTGGATATGTATACAAGTAAAGTAAGCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAACATGAC‑AGCGTACCGTTTACCCGGCGAGGAATGGAACCAGCGTGTCATCAGTCAGTGAGATGATTGCAGCAACGTGTTGGTCTTAACGTACCAGTTCGATGAAtgtgacg                                                                                                                                                                                                        <  2:157080/236‑4 (MQ=255)                                             gCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAAcc                                                                                                                                                                                           <  1:237967/251‑1 (MQ=255)                                                                                aagtCGCATAATAGCCCCCTGTTGAATATACTTGTTATTTATCCAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGAGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTGTGATGAATGTGTCGTTGACCGTCAACCCATAGAATTCCGGTCCGTTTACAGAACAGAATGCt                                                                                                                                                        <  2:312339/248‑1 (MQ=255)                                                                                   tCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTc                                                                                                                                                      <  2:529421/250‑1 (MQ=255)                                                                                     gCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCaa                                                                                                                                                    >  1:246901/1‑250 (MQ=255)                                                                                        taataGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCAGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGtg                                                                                                                                                >  1:392244/1‑251 (MQ=255)                                                                                        taataGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGACACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATACTTCAAAgcg                                                                                                                                                >  1:82518/1‑249 (MQ=255)                                                                                                     ttGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCTTAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGcattc                                                                                                                                   >  1:18001/1‑251 (MQ=255)                                                                                                             aCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATttcttc                                                                                                                           <  2:246901/251‑1 (MQ=255)                                                                                                             aCTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATttctt                                                                                                                            <  1:340491/250‑1 (MQ=255)                                                                                                                ggTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCaaa                                                                                                                        <  2:265590/251‑1 (MQ=255)                                                                                                                ggTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCaa                                                                                                                         <  1:432192/250‑1 (MQ=255)                                                                                                                    atttatACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACg                                                                                                                    <  2:155643/251‑1 (MQ=255)                                                                                                                               tAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACt                                                                                                         >  1:620934/1‑251 (MQ=255)                                                                                                                                aaaaTAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCAGCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTg                                                                                                        <  1:603880/251‑1 (MQ=255)                                                                                                                                        cTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGAATTCATTTCTTCAAAGACGGTAGCGTAACTGCCAAgcgc                                                                                                >  1:166575/1‑251 (MQ=255)                                                                                                                                            tGACAACAGGCAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAACGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGtt                                                                                            >  2:91657/1‑251 (MQ=255)                                                                                                                                             gACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTg                                                                                           <  1:273971/251‑1 (MQ=255)                                                                                                                                              acaacaGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTgg                                                                                          >  1:640502/1‑251 (MQ=255)                                                                                                                                                aacaGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTgggg                                                                                        >  1:98566/1‑251 (MQ=255)                                                                                                                                                aacaGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGCACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTgggg                                                                                        >  2:639786/1‑251 (MQ=255)                                                                                                                                                           aCGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAagc                                                                               >  2:556635/1‑249 (MQ=255)                                                                                                                                                                    aTTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCAAGCGCGATTGGGGCGTTGAAGCAGCCCgcgc                                                                      >  2:633889/1‑249 (MQ=255)                                                                                                                                                                                   cAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGctctc                                                      >  2:163587/1‑251 (MQ=255)                                                                                                                                                                                      cGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCttt                                                   >  1:178351/1‑251 (MQ=255)                                                                                                                                                                                       gTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCtttt                                                  >  2:330990/1‑250 (MQ=255)                                                                                                                                                                                         aCCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCg                                                <  1:441039/251‑1 (MQ=255)                                                                                                                                                                                            gTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATg                                             <  2:98566/251‑1 (MQ=255)                                                                                                                                                                                                  ccGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCATCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGc                                       >  1:402313/1‑251 (MQ=255)                                                                                                                                                                                                      cGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCAtgt                                   >  1:21338/1‑251 (MQ=255)                                                                                                                                                                                                                    aGCGTGTCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGt                     >  1:511638/1‑251 (MQ=255)                                                                                                                                                                                                                       gtgtCATCAGTCAGTGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTAcc                  >  1:264632/1‑251 (MQ=255)                                                                                                                                                                                                                                   agtGCGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGGTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGTTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGAGGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCg      <  2:316210/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGATGTTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTa  <  1:91657/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                      GAATACGGCGGGAAAGTTCGCCAGCCAGGGCGTCAATAGCCCCAGCTGGCAATGGAGAAGTTTTCGCTATGGTGACTTCAATTCGCATAATAGCCCCCTGTTGAATATACTGGTTATTTATACAGGTAAAATAACCTAATGACAACAGGAAGCTACGATTTTTATTGTTTAAC‑GGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTA  >  NC_000913/1121400‑1121879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑