Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,076,800 C→T A695T (GCG→ACG)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,076,8000CT98.5% 207.6 / NA 67A695T (GCG→ACG) putAfused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (29/37);  minor base A (1/0);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ATACACACCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTG  >  NC_000913/1076559‑1077043
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   
atanccaccacAGGCCCTAATGGACGGTGGGTTTCGTGAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGGTGCTGCATCTGGCTTGCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAAc                                                                                                                                                                                                                                            <  2:593831/246‑1 (MQ=255)
  acacacCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCg                                                                                                                                                                                                                                          <  1:196051/251‑1 (MQ=255)
      accacAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACt                                                                                                                                                                                                                                      >  1:482922/1‑251 (MQ=255)
        cacaGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACttt                                                                                                                                                                                                                                    >  2:628382/1‑251 (MQ=255)
                cTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGcc                                                                                                                                                                                                                            >  1:64756/1‑251 (MQ=255)
                    tGGACGGTGGCTTACGTTAGCGAAATCATCCCGCACATGGCTGGCGTAGGAGGGGCGAAAATAGACCGCATCGCGCACGTCGGCGATGGAGTTATTGAAGGTTTTTCCGGCCTCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCCAAATCGATGCGT‑GTTAATCCGGTGGTGTGGCACATCAGATTGTTGTGTTATTAAACGCACTTTCCAggtactgtt                                                                                                                                                                                                                        >  2:42570/1‑243 (MQ=255)
                            gggTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTc                                                                                                                                                                                                                 <  1:494847/250‑1 (MQ=255)
                            gggTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCa                                                                                                                                                                                                                <  1:584480/251‑1 (MQ=255)
                                 tCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACgcgg                                                                                                                                                                                                           >  2:617233/1‑251 (MQ=255)
                                     tAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTg                                                                                                                                                                                                       >  1:347372/1‑251 (MQ=255)
                                          aaaTCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCTCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTc                                                                                                                                                                                                  <  1:145921/251‑1 (MQ=255)
                                              cagcccGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAAGCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACg                                                                                                                                                                                               <  2:68294/247‑1 (MQ=255)
                                               atcCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGc                                                                                                                                                                                              >  2:249387/1‑250 (MQ=255)
                                                   cgcgccTGTCCGGTGTAGTAGTGGAGAAAATCTACCACTTCGCGCACTGCGGCAATGGCGTTACGGAATGTTGTTCCGGCCTCACGAGCCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCTCGGCAGCGCGGTGCAAAATCGCTGCGC‑TTTCAGCCGGAGTCGTTGTAAATCAGATTGGCGTGTTATTAATCGCACATGCCAGCGCCTGTTCTACTGCGCGCGGTGTTGCTTCACGCACATa                                                                                                                                                                                         <  2:85054/247‑1 (MQ=255)
                                                     cACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGc                                                                                                                                                                                       <  1:116087/251‑1 (MQ=255)
                                                                   agtagtGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGGTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGAAAACCAGATTGGCGTGTTATTACCCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCttt                                                                                                                                                                          <  2:18627/250‑1 (MQ=255)
                                                                    gtagtGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCg                                                                                                                                                                        <  1:628382/251‑1 (MQ=255)
                                                                    ggagtGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCg                                                                                                                                                                        <  2:344598/249‑1 (MQ=255)
                                                                         gtagaAAATCGCCCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTACCCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCGCGCACATAGCCCACAATATCTTTCGGTTcc                                                                                                                                                                   <  2:352318/249‑1 (MQ=255)
                                                                         ggAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTcc                                                                                                                                                                   <  1:526875/251‑1 (MQ=255)
                                                                           agaAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCATGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGc                                                                                                                                                                                       <  1:41362/229‑1 (MQ=255)
                                                                           agaAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCATGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGc                                                                                                                                                                                       >  2:41362/1‑229 (MQ=255)
                                                                               aaTCGACCTCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAggg                                                                                                                                                             <  1:305016/251‑1 (MQ=255)
                                                                                  cGACCGCTTCGCGCACTTCGGCAATTGCGTTACTGAAGGTTTTTCCGGCCGCACGCACCAGAATGCCAATCAGTTGCTGCATCTGGCTTACCATCAGCACGGCAGCGCGGTGCATAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTTGCTTCACGCACATAGCCCTCAATATCTTTCGGTTCCGCAGGGTta                                                                                                                                                          <  2:114564/251‑1 (MQ=255)
                                                                                  cGACCGCTTCGCGCACGTCGGCAATGGCGTTACTGAAGGTTTTGCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTta                                                                                                                                                          <  2:144389/251‑1 (MQ=255)
                                                                                      cGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTtaata                                                                                                                                                       >  2:235294/1‑250 (MQ=255)
                                                                                         ttCGCGCACTTCGGCAATGGCGTCACTGAAGGTTTTCCCAGCCTCAAGCACCAGATTACCAATCAGTTGCTGCATATGGCTTTTCATCAGCACGGAAGTGCGGTGTAAAACAGCTGCGC‑GTTCAGCCAGAGGAGTAGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCACACAATATCTTTCGGTTCCGCAGGGTTAACAACgg                                                                                                                                                   >  2:385286/1‑251 (MQ=255)
                                                                                                    cagCAATGGCGTTACTGAAGGTTTTTCCTGCCTCACGCACCAGAATACCAATTAGTTTCTGCATCTGGCTTGCCATCAGCACGGCAGCGCGGTGCAAAATCGCTCCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCCCTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCCCGCACATATCCCACAATATCTATCGGTTTCTCAGGGTTAATAACGGGCCACAtctc                                                                                                                                         <  2:1966/248‑1 (MQ=255)
                                                                                                     ggCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCAc                                                                                                                                       <  1:320255/251‑1 (MQ=255)
                                                                                                     ggCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCAc                                                                                                                                       <  1:443739/251‑1 (MQ=255)
                                                                                                      gcgaTGGCGTTCCTGAAGGTTTGTCCGGCCTCACGCACCAGAATGCCAATCAGTTGCTGCATCTTGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCAcc                                                                                                                                      <  2:564508/248‑1 (MQ=255)
                                                                                                        aaTGGCGTTACTGAAGGTTTTACCGGCCTCACGAACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGAAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCAGTTTTACTTCAAGCGGAGTGGCTTAACGCACATAGGCCACAATATCTTTAGGTTCCGCAGGGTTAATAACggatgacaacacacctg                                                                                                                                    >  1:303370/1‑236 (MQ=255)
                                                                                                          tGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTACAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGcc                                                                                                                                  >  2:519269/1‑251 (MQ=255)
                                                                                                           ggCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGAAATCTCACCTGCCg                                                                                                                                 >  1:319116/1‑251 (MQ=255)
                                                                                                                 ccTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAACCCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCCCCTGCCGCTACCg                                                                                                                           <  2:1451/250‑1 (MQ=255)
                                                                                                                    gacggTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTCCCATCAGCCCGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCCCTTTCCAGCGCCTGTTCTACTTCCCGCGGCGTGGCTTCACGCACATAGCCCACAATCTCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGgtt                                                                                                                        <  2:545832/248‑1 (MQ=255)
                                                                                                                     aaGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGgttg                                                                                                                       >  1:177979/1‑251 (MQ=255)
                                                                                                                     aaGGTTTTTCCGGCCTCACGCACCAGAATAACAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGAGCACAATCGCTGCGC‑GATCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCAAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGCTAATAACGGGCGACATCTCACCTGCCGTTACCGgttg                                                                                                                       >  2:542493/1‑251 (MQ=255)
                                                                                                                       ggTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGgttgtt                                                                                                                     <  1:627713/251‑1 (MQ=255)
                                                                                                                          ttttCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTcc                                                                                                                   <  1:555793/250‑1 (MQ=255)
                                                                                                                                    tCACGCACCAGAATACAAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTCCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACTCGGCGTGGCTTCACGCACATAGACCACACTATCTTTCGGGTCCGCAGGGTTAATAACGGACGACATATCACCTGCCGCGACCGGTTGTTCCAGCATTgcca                                                                                                         >  2:207710/1‑247 (MQ=255)
                                                                                                                                         cACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCt                                                                                                   >  1:413204/1‑251 (MQ=255)
                                                                                                                                         cACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGGTGTTCCAGCATTGGCAAGGCCt                                                                                                   >  1:529693/1‑251 (MQ=255)
                                                                                                                                          aCCAGAATACCGATCAGTTGATGCATCTGGCTTTCCATCCGCACGGCAGCGCGGTGCACAATCGCTGCGC‑GTTCAGCCGGAGGCCTGACAAACCAGATTAGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATACCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGTCATCTCACCTGCCGCTACCGGTTGTTCCAGCATTCGCCAGGCCTg                                                                                                  >  2:313590/1‑251 (MQ=255)
                                                                                                                                            cAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCAGCAGGGTTAATAACGGGAGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCGAGGTCTga                                                                                                 >  2:309689/1‑249 (MQ=255)
                                                                                                                                                aTACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCAttt                                                                                            >  1:186642/1‑251 (MQ=255)
                                                                                                                                                   ccAATCAGTTGCTGCATCTGGCTTTTCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGTCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGGTCTACTGCACTCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCGGCAGGGTTAATAACGGGCGACATCTCAcc                                                                                                                                      <  2:145862/206‑1 (MQ=255)
                                                                                                                                                   ccAATCAGTTGCTGCATCTGGCTTTCCATAAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGAGTTATTAACCGCACTTTCCAGCGCCTGTTCTAATTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCAcc                                                                                                                                      >  1:145862/1‑206 (MQ=255)
                                                                                                                                                      aTCAGTTGCTGCATCTGGTTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCGGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAg                                                                                      <  2:466654/251‑1 (MQ=255)
                                                                                                                                                         aGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGc                                                                                   >  1:332770/1‑251 (MQ=255)
                                                                                                                                                                      ggCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAgg                                                                      <  1:68681/251‑1 (MQ=255)
                                                                                                                                                                      ggCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAg                                                                       <  1:292078/250‑1 (MQ=255)
                                                                                                                                                                       gCTTTTCATCATCACGGCAGCGCGGTGCAAAATCGCTGCGCTGTT‑AGCCGGAGGGGTGGCAAACCAGATGGGCGTGTTAATAACCGCACTTTCCAGCGCCTTTTTTCCTTCACCCGGCGTGGCTTCACGCACATAGCCCACAATATAGTGCGGTTCCGCGGGGTTAAGAACGGGCCACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATGGAGCAggg                                                                     <  2:29796/251‑1 (MQ=255)
                                                                                                                                                                                  gCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCCCTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGc                                                          <  1:178329/251‑1 (MQ=255)
                                                                                                                                                                                         aGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCCCTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGccc                                                   >  2:138660/1‑250 (MQ=255)
                                                                                                                                                                                                  cAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCTTGTTATTACCCGCACTTTCCAGCGCGTGTTCGACTGCACGCGGCGAGGCTTCACGCACATAGCCCGCAATTTCTTTCGGTTCCGCAGGGTTAAAAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTATTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGtt                                          <  1:385286/251‑1 (MQ=255)
                                                                                                                                                                                                         gatGCGC‑GTTCAGCCGGAGGCGTGGGAAACCAGATTGGCGTGTTATTAACCTCACTTTCCAGCGTCTGTTCTGCGTCACGCGGCGTGGTTTCACGCACACACCCCCCAATATCTTTCTGTTCCGCAGGGTTAATAACGGGGCACTGCGCCTCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCTAGGCGGTGTTCGTTAGc                                   <  1:453005/249‑1 (MQ=255)
                                                                                                                                                                                                         gCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATTTCTTTCGGTTCCGCAGGTTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATGTTTGCAGTGCACTATTGAGCAGGGCAGAGGATAGCGAGGCCAGGCGGTGTTCGTTAGc                                   <  2:64756/251‑1 (MQ=255)
                                                                                                                                                                                                            gcgc‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCg                                  >  1:555558/1‑249 (MQ=255)
                                                                                                                                                                                                             cgc‑gTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCg                                  >  1:319351/1‑248 (MQ=255)
                                                                                                                                                                                                                   tCAGCCGGAGGCGTGGCAACCTCGATGGGCGTGTTATTACCCGCGCTTCCCAGCGCCTTTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTGCGTTAGCGAGATCCa                           <  2:547931/250‑1 (MQ=255)
                                                                                                                                                                                                                     aGCCGGAGGCGTGGCAAACCAGATTGGTGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCCCGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCGAGATCCAGcc                        <  2:332770/251‑1 (MQ=255)
                                                                                                                                                                                                                           aGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCCCTTTCCAGCGACTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCGAGATCCAGCCCTGCCg                  >  1:377919/1‑251 (MQ=255)
                                                                                                                                                                                                                           aGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCAACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCGAGATCCAGCCCTGCCg                  <  1:155494/251‑1 (MQ=255)
                                                                                                                                                                                                                           aGGCGTGGCAAACCAGATTGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGACCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGTGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCGGGGACCAGCCCTGCCg                  >  2:36417/1‑251 (MQ=255)
                                                                                                                                                                                                                                tGGCAAACCAGATTGGCGTGCTATTAACCGCACTTTCTACAGCCTGGTGAACTTCACGAGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCCCAGGGTTAATAACGGGCGACATCTCCCCTGCCCCCACCGGTTGTTCCAGCATTGGCAAGACCTGCCATTTTTGCAGTGAACTATTAGGCAGGGCAGCGGCGAGCGACGCCCGGCGGTGTTCGTTAGCGCGACCCAGCGCTGCCGAGTTg             >  2:660025/1‑251 (MQ=255)
                                                                                                                                                                                                                                            ttGGCGTGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCggg  >  1:586667/1‑248 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   
ATACACACCACAGGCCCTAATGGACGGTGGGTTTCGTTAGCGAAATCATCCCGCACCTGTCCGGCGTAGTAGTGGAGAAAATCGACCGCTTCGCGCACTTCGGCAATGGCGTTACTGAAGGTTTTTCCGGCCTCACGCACCAGAATACCAATCAGTTGCTGCATCTGGCTTTCCATCAGCACGGCAGCGCGGTGCAAAATCGCTGCGC‑GTTCAGCCGGAGGCGTGGCAAACCAGATTGGCGCGTTATTAACCGCACTTTCCAGCGCCTGTTCTACTTCACGCGGCGTGGCTTCACGCACATAGCCCACAATATCTTTCGGTTCCGCAGGGTTAATAACGGGCGACATCTCACCTGCCGCTACCGGTTGTTCCAGCATTGGCAAGGCCTGCCATTTTTGCAGTGCACTATTGAGCAGGGCAGAGGAGAGCGAGGCCAGGCGGTGTTCGTTAGCGAGATCCAGCCCTGCCGAGTTGTCGCGCCCGTG  >  NC_000913/1076559‑1077043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑