Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,233,152	G→A	L28F (CTT→TTT)	lysC ←	aspartate kinase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,233,152	0	G	A	100.0%	225.5 / NA	66	L28F (CTT→TTT)	lysC	aspartate kinase III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/36);  total (30/36)

CGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACA  >  NC_000913/4232907‑4233382                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       cgcCGGAAACGTTGCCAGCGCCGCCTCTTCTGCCAGAACAATAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGCCCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACaa                                                                                                                                                                                                                                   <  2:239612/251‑1 (MQ=255)  gcCGGAGACGTTGCCCGCGCCGCCGCTTCTGCCAGAACAATAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAat                                                                                                                                                                                                                                  <  2:112813/251‑1 (MQ=255)   ccGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAATAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAat                                                                                                                                                                                                                                  >  1:361146/1‑250 (MQ=255)     ggAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAATAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATc                                                                                                                                                                                                                               <  1:547470/251‑1 (MQ=255)       ggaCGTTGCCCGCGCCGCCGCTTCTGCCAGAACAATAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAg                                                                                                                                                                                                                             <  2:464480/250‑1 (MQ=255)       agaCGTTGCCAGCGCCGCCGCTTCTGCCAGAACAATAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATc                                                                                                                                                                                                                               >  1:344025/1‑249 (MQ=255)         aCGTTGCCAGCGCCGCCGCTTCTGCCAGAACAATAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAgcg                                                                                                                                                                                                                           >  1:16823/1‑251 (MQ=255)           gTTGCCAGCGCCGCCGCTTCTGCCAGAACAATAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCt                                                                                                                                                                                                                         <  2:645017/251‑1 (MQ=255)              gCCAGCGCCGCCGCTTCTGCCAGAACAATAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGc                                                                                                                                                                                                                       <  2:520148/250‑1 (MQ=255)                  gcgcCGCCGCTTCTGCCAGAACAATAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCGAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTc                                                                                                                                                                                                                  <  1:463579/251‑1 (MQ=255)                         cgcTTCTGCCAGAACAATAATGTTCTCAAGCAGACATTAAATCTCTTCACGGATCACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGAAAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACAAGCAGAGGCCGAGAGGACAACAAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTAATGGCGt                                                                                                                                                                                                           >  2:392162/1‑251 (MQ=255)                             tCTGCCAGAACAATAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCCGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCATCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACTCACGGTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCa                                                                                                                                                                                                         <  1:251764/249‑1 (MQ=255)                                gCCAGAACAATAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCaaaa                                                                                                                                                                                                      <  1:436259/249‑1 (MQ=255)                                   aGAACAATAATGTTCTCCCGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGc                                                                                                                                                                                                 >  2:12072/1‑251 (MQ=255)                                            aTGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGt                                                                                                                                                                                        <  1:112833/251‑1 (MQ=255)                                                tcgccAGAAGACGTCCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACGGGATGTTGCGTCTATCGTCGAGTTTTTCGAATCGCTCGGCAGGTGCCAGTCCTTCAGCTAAAGCCACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAACCGCTCGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGTGTCAAAATCAGCTACTCTgatgcc                                                                                                                                                                                     <  2:66496/247‑6 (MQ=255)                                                      gcagACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAAt                                                                                                                                                                              <  1:129376/251‑1 (MQ=255)                                                               cAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGac                                                                                                                                                                      >  2:186737/1‑250 (MQ=255)                                                                aaTCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGaca                                                                                                                                                                     >  2:534554/1‑250 (MQ=255)                                                                   ctctTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCAAGGTTCCAGTCCTTCAGCTAAAGCGACAAGCAGATTAGTGATACCAGCAAAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGATGCGGTTCATGGCGACAAAATCAGCTACGATGGAACCGCCAAATTTGGAGacaaca                                                                                                                                                                  >  2:190139/1‑250 (MQ=255)                                                                       tcgcGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGAACAACTAGGCGCACGTTGGCATCAGAAAACACAATATCAgc                                                                                                                                                                                                                            <  1:540307/185‑1 (MQ=255)                                                                       tCACGGATAACGTTCGGGTAACGCAGACGGTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCAAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAgc                                                                                                                                                                                                                            >  2:540307/1‑188 (MQ=255)                                                                        cACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTc                                                                                                                                                             <  1:102963/250‑1 (MQ=255)                                                                        cACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCa                                                                                                                                                            <  2:126985/251‑1 (MQ=255)                                                                          cGGATAACGTTCAGGCAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGGTCCAGTCCTTCAGCTAAAACGACCAGCAGCTTAGTGATCCCAGCAGAAGCCGAGAGAGCACCTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAACTCAGCTACACTGGTACCGCCACATTTGGAGACAACAATTTc                                                                                                                                                             >  2:365823/1‑248 (MQ=255)                                                                           ggATAACGTTCGGTTAACGAAGCCTTTCCAGAATTGCAAACTGGATGTTGCGGATAGCGTCTAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGTAGACAACAATTTCAGAc                                                                                                                                                         <  2:660611/251‑1 (MQ=255)                                                                           ggATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGAc                                                                                                                                                         >  2:419488/1‑251 (MQ=255)                                                                               aaCGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCg                                                                                                                                                                                                            >  1:15043/1‑196 (MQ=255)                                                                               aaCGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCg                                                                                                                                                                                                            <  2:15043/196‑1 (MQ=255)                                                                                               aGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGCCAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAggg                                                                                                                                     >  2:641299/1‑251 (MQ=255)                                                                                                    ttCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATcc                                                                                                                                >  1:221482/1‑251 (MQ=255)                                                                                                      ccAGAATGGCAAACTGGATTTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCa                                                                                                                               <  1:629021/250‑1 (MQ=255)                                                                                                      ccAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCa                                                                                                                               <  1:534554/250‑1 (MQ=255)                                                                                                        aGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCAttt                                                                                                                            <  1:365468/251‑1 (MQ=255)                                                                                                            ggtcaaatgggATGTTACGGATAGCGTCGAGTTTTTCTAATCGCTCGCCAGTTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATAAGTGATACCAGCCGAAGCCGAGAGGACACCTAAACGCACGTGGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCTGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATATCTACCCCGTGTCAGGGGATCCATTTTCAg                                                                                                                        <  2:232582/242‑1 (MQ=255)                                                                                                                aaaCTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCt                                                                                                                                                                                           >  2:330487/1‑180 (MQ=255)                                                                                                                aaaCTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCt                                                                                                                                                                                           <  1:330487/180‑1 (MQ=255)                                                                                                                      gATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGCAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGcac                                                                                                               >  2:220699/1‑250 (MQ=255)                                                                                                                             cGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGa                                                                                                         >  1:446997/1‑249 (MQ=255)                                                                                                                                  aGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCgg                                                                                                  <  1:447954/251‑1 (MQ=255)                                                                                                                                              ttCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTgg                                                                                       <  2:243028/250‑1 (MQ=255)                                                                                                                                               tCNAATCGCTCGCCAGGTTCCAGGCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTggg                                                                                      <  2:588440/250‑1 (MQ=255)                                                                                                                                               tCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACATCTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTggg                                                                                      <  2:251092/250‑1 (MQ=255)                                                                                                                                               tCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATGTCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTggg                                                                                      <  1:28624/250‑1 (MQ=255)                                                                                                                                                 gAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGgcgc                                                                                   >  2:377556/1‑251 (MQ=255)                                                                                                                                                     cgatcgacAGGTTCAAGTCCTTCAGCTAAAGCGACCAGCAGAATAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTCCCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCaga                                                                                <  1:452678/243‑1 (MQ=255)                                                                                                                                                           ccAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATAc                                                                         >  1:419346/1‑251 (MQ=255)                                                                                                                                                                    attCCTTCAGCTAAAGCGACCAGAAGATTAGTGATAGCAGCAGAAGCCGAGAGGAGAACTAAACGCACGTTGGCATCAGAAAACCCAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGAGTCAGGGGCTCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGAGGGCGCAGAGCGATACTTCGctac                                                                 >  2:377891/3‑250 (MQ=255)                                                                                                                                                                     gTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGGGAGGACAACTAAACGCACGTTGGCATAAGAAAACACAATATCAGCGCGGCAGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGTGCGCAGAGCGATACTTCGctact                                                                >  2:283177/1‑250 (MQ=255)                                                                                                                                                                           cAGCTAAAGCGACCAGCAGATTAGTGAAACCAGCAGAAGCCGAGAGGACAACTAAACGCCCGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATTGCGTCAAAATCAGCTACTCTGGTACCGCCAAATTTGTAGACAACAATTGCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGATCGATACTTCGCTACTATTTTc                                                          <  2:364428/250‑1 (MQ=255)                                                                                                                                                                                    cGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTTTCACCCAGAAGt                                                <  1:186737/251‑1 (MQ=255)                                                                                                                                                                                     gACCAGCAGATTAGTGAGACCAGCAGAAGCCGAGAGGACAACGAAACGCACGTTGGCATCATAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGAGCCATTTTCAGCCTTGGCACAAGGGAAGAGCTTAAGACGGGTGGGCGCAGAGCGAGACTCCGctacgagttgagcccagaagtg                                               <  2:36124/251‑19 (MQ=255)                                                                                                                                                                                       ccAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGc                                                                              >  2:574770/1‑218 (MQ=255)                                                                                                                                                                                       ccAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGc                                                                              <  1:574770/218‑1 (MQ=255)                                                                                                                                                                                         agcagATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTTTCACCCAGAAGTGCTcc                                           >  1:159823/1‑251 (MQ=255)                                                                                                                                                                                         agcagATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTTTACCCCAGAAGTGCTcc                                           >  2:138046/1‑251 (MQ=255)                                                                                                                                                                                              aTTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTGCCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTTTCACCCAGAAGTGCTCcaccac                                      <  2:44808/251‑1 (MQ=255)                                                                                                                                                                                               ttAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTTTCACCCAGAAGTGCTCCACCACt                                     >  2:297260/1‑251 (MQ=255)                                                                                                                                                                                                   tGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGAACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGCAGACGGGTGGGCGCCGACCGTTACTTCGCTACTATTTTCACCCAGAAGTCCTCCACCACTTGCg                                 >  2:50330/1‑251 (MQ=255)                                                                                                                                                                                                          agcagAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGcc                          >  1:579803/1‑251 (MQ=255)                                                                                                                                                                                                           gcagAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTTTCACCCAGAAGTGCTCCCCCACTTGCGAAACGccc                         <  1:109707/251‑1 (MQ=255)                                                                                                                                                                                                           gcagAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGccc                         <  1:481584/251‑1 (MQ=255)                                                                                                                                                                                                            cagAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCg                        >  2:70241/1‑251 (MQ=255)                                                                                                                                                                                                                      gagGACAACTAAACGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACg              >  2:574430/1‑251 (MQ=255)                                                                                                                                                                                                                        ggACAACTAAAAGCACGATGGCATCAGAAAACACAATATCAGCGCTGCGGATCATGGCGTCAAAATCAGCTACGCTGGTACAGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGCGCGGAAGACTGGAGGGCGCAGAGCGATACTTCGCTACTATATTCACCCAGAAGTGCTCCACCACTTGCGAAAAGCACGACTGCGAACGCt            >  2:365994/1‑251 (MQ=255)                                                                                                                                                                                                                                  aCGCACGTTGGCATCAGAAAACACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTTTCACCCAGAAGTGCTCCACCACTTGCGAAACTCCCGACTGCGAACGCTTCTGGTGACa  <  2:260501/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       CGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGTTCAATCTCTTCACGGATAACGTTCGGGTAACGCAGACGTTCCAGAATGGCAAACTGGATGTTGCGGATAGCGTCGAGTTTTTCGAATCGCTCGCCAGGTTCCAGTCCTTCAGCTAAAGCGACCAGCAGATTAGTGATACCAGCAGAAGCCGAGAGGACAACTAAACGCACGTTGGCATCAGAAAGCACAATATCAGCGCTGCGGTTCATGGCGTCAAAATCAGCTACGCTGGTACCGCCAAATTTGGAGACAACAATTTCAGACATAACTACCTCGTGTCAGGGGATCCATTTTCAGCCTTGGCACAAGGGAAGAGCGGAAGACGGGTGGGCGCAGAGCGATACTTCGCTACTATTTTCACCCAGAAGTGCTCCACCACTTGCGAAACGCCCGACTGCGAACGCTTCTGGTGACA  >  NC_000913/4232907‑4233382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑