Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,693,035	G→A	A410V (GCC→GTC)	uidB ←	glucuronide:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,693,035	0	G	A	100.0%	107.0 / NA	34	A410V (GCC→GTC)	uidB	glucuronide:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/14);  total (20/14)

GTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTCACCGTATTCTACGGTATCAGCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAAT  >  NC_000913/1692796‑1693273                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               gTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGAtgtg                                                                                                                                                                                                                                     <  2:329575/251‑1 (MQ=255)        cAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGc                                                                                                                                                                                                                              >  2:64303/1‑251 (MQ=255)            ggCAGCCGTCAGGCAAATAACGGCCATGGCCACTCTTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCat                                                                                                                                                                                                                          >  1:536429/1‑251 (MQ=255)                  cGTCAGGCAAATAACGGCCATGGCTACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTGATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAAc                                                                                                                                                                                                                    <  2:215262/251‑1 (MQ=255)                   gTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATTCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGGCCGGATGCCCATAATAACt                                                                                                                                                                                                                   >  2:548038/1‑251 (MQ=255)                         cAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGc                                                                                                                                                                                                             >  1:553808/1‑251 (MQ=255)                                                ttttCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGGTTGCACCTGATTGGCGatat                                                                                                                                                                                       <  2:67124/250‑1 (MQ=255)                                                             gcaacTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAAccc                                                                                                                                                                          <  2:16478/246‑1 (MQ=255)                                                                 cTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGGACTAAGACAATTGATGTGCGGCTGCCCATAATAACTTCCGCCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCaaa                                                                                                                                                                      >  2:488747/1‑250 (MQ=255)                                                                      attttCTGATTATTTTTATTGACTATTAATTAGGGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGACTTTTTTATCCGTGAGCGGATAACACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCACATAATAACTTCGGGCGTTTGCACCTGTTTGGCGATATATCAGCTTAACGCCAAAATaacg                                                                                                                                                                >  2:17864/4‑249 (MQ=255)                                                                                    tttATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAAcc                                                                                                                                                  >  2:191209/1‑251 (MQ=255)                                                                                        ttGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCg                                                                                                                                              >  2:420123/1‑251 (MQ=255)                                                                                                                                    tttttACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAg                                                                                                    <  2:406962/249‑1 (MQ=255)                                                                                                                                      tttACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTg                                                                                                 <  2:177191/250‑1 (MQ=255)                                                                                                                                           gATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGAAAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCAGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACAGAATTTACGGGCAAATGAGAATAGTGAATAGGTGAGCCCt                                                                                           >  2:280234/1‑251 (MQ=255)                                                                                                                                            aTTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCtt                                                                                          <  2:217491/251‑1 (MQ=255)                                                                                                                                              tATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTc                                                                                         <  2:436400/250‑1 (MQ=255)                                                                                                                                               aTCAATTTCAACCACGATTTCTTTGAATTTTTTATCAGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCa                                                                                        >  2:310318/1‑250 (MQ=255)                                                                                                                                                 cAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAAtt                                                                                     >  1:385964/1‑251 (MQ=255)                                                                                                                                                            aCGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAAACTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTAGCACGCCGGt                                                                          >  1:279161/1‑251 (MQ=255)                                                                                                                                                              gATTGCTTGGAATTGTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGGACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCa                                                                        <  2:145404/251‑1 (MQ=255)                                                                                                                                                                      ttaaTTTTTTATCCGTGATCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAACGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTc                                                                 <  2:385964/248‑1 (MQ=255)                                                                                                                                                                                aTCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTCACCGTATTCt                                                       >  2:154443/1‑250 (MQ=255)                                                                                                                                                                                 tCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATACGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTCACCGTATTCTAc                                                     >  1:358756/1‑251 (MQ=255)                                                                                                                                                                                  ccGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTCACCGTATTCTACg                                                    <  1:548038/251‑1 (MQ=255)                                                                                                                                                                                              aaaaCCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTCACCGTATTCTACGGTATCAGCTTcc                                        <  2:347745/251‑1 (MQ=255)                                                                                                                                                                                               aaaacAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGAGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTACGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGGGAATCGGTGAGCTCTTCAATTCGCACGCCGGTCAGGCATTCACCGTATTATACGGTATAAGCTTcc                                        >  2:638151/5‑250 (MQ=255)                                                                                                                                                                                                  ccAGATAATAACGAATGCCAGTAGCATAAAGCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTCACCGTATTCTACGGTATCAGCTTCCAgcg                                    <  2:125304/251‑1 (MQ=255)                                                                                                                                                                                                      ataataACGAATGCCAGTAGCCTAAATCCGCGAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACTGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTCACCGTATTCTACGGTATCAGCTTCCAGCGccc                                 >  1:245407/1‑250 (MQ=255)                                                                                                                                                                                                              gAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTCACCGTATTCTACGGTATCAGCTTCCAGCGCCCACATCACgg                        <  2:553808/251‑1 (MQ=255)                                                                                                                                                                                                              gAATGCCAGTAGCATAAATCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTCACCGTATTCTACGGTATCAGCTTCCAGCGCCCACATCACgg                        >  2:372421/1‑251 (MQ=255)                                                                                                                                                                                                                             aaaTCCGCAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTCACCGTATTCTACGGTATCAGCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCt         >  1:19456/1‑251 (MQ=255)                                                                                                                                                                                                                                   gCAAGGGACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTAACCGTATTCTACGGTATCAGCTTCCCGCGCACACATCACGGTCCTGGTAACGCCCTGAcaa    >  2:397376/1‑248 (MQ=255)                                                                                                                                                                                                                                    cAAGGTACTAAGACAATTGATGTGCGGATGCCCATAATAACTTCAGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTCACCGTATTCTACGGTATCAGCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAAt  >  2:645834/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               GTAAGGCCAGAGGCAGCCGTCAGGCAAATAACGGCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGAACCTCCGATTGCCTGACCGCATTTACGGGTAAATGAGAATAGTGAATAGGTGAGCCCTTCAATTCGCACGCCGGTCAGGTATTCACCGTATTCTACGGTATCAGCTTCCAGCGCCCACATCACGGTCATGGTAACGCCCTGACCAAT  >  NC_000913/1692796‑1693273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑