Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,227,310	G→A	Q359Q (CAG→CAA)	ygjI →	putative transporter YgjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,227,310	0	G	A	95.6%	135.1 / ‑6.3	45	Q359Q (CAG→CAA)	ygjI	putative transporter YgjI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (30/13);  minor base C (2/0);  total (32/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTT  >  NC_000913/3227068‑3227545                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            cTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCtgat                                                                                                                                                                                                                                     >  1:192497/1‑251 (MQ=255)       gAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGCAATCCCGGAA‑GGCATCTTTGGTAAGACAACCGTCGTACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGAGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATATCGTGCACGATCTGATGAATAc                                                                                                                                                                                                                               >  2:156373/1‑251 (MQ=255)            gtggctgaATCGCTTTGTCGGTCTGGTGTCCTTTGCCGCGATGTTCGGTTCCATGCTGATGGGGACCGCAACGCCGGGGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGAGTTCCGGCGCGCGCAGCGTGGATCCA‑GCTCCTGATCGTCATCCCGCTGATTATTATCCCGGTGCTCGCTTACAATGCCGTGCAAGATCTGAGGTATACtatta                                                                                                                                                                                                                          <  2:96081/246‑1 (MQ=255)             ttGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACtattat                                                                                                                                                                                                                         >  1:515943/1‑251 (MQ=255)               gatgaATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCATGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATAATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTattatt                                                                                                                                                                                                                        >  1:658496/1‑250 (MQ=255)                  gaATCGCTTTTTCGGTCTGGTGTCTTTTTCCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATTCA‑GTTCCTTATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTACTAata                                                                                                                                                                                                                    <  2:105300/251‑1 (MQ=255)                   aaTCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAatat                                                                                                                                                                                                                   <  1:582862/251‑1 (MQ=255)                         tttGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGAccgc                                                                                                                                                                                                             >  2:269319/1‑251 (MQ=255)                                              aCCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAACCCGTCGAACTGAACGAACACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTTATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgcc                                                                                                                                                                                        <  1:411323/251‑1 (MQ=255)                                              aCCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgcc                                                                                                                                                                                        >  1:660016/1‑251 (MQ=255)                                              aCCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgcc                                                                                                                                                                                        >  2:412897/1‑251 (MQ=255)                                                cgcgATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGCTGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGt                                                                                                                                                                                      >  2:254975/1‑251 (MQ=255)                                                         cGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATtcatc                                                                                                                                                                              >  1:28640/1‑250 (MQ=255)                                                          tgTTCGCTGATGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAGATCCCGGAA‑GGCATCTTGGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGAGGAGCCA‑GTTCCTGATCGACATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGAACTGATGAATACTATTATTAATATTACCGCTGCAGCGTCCATGCTTCCGCCGTTATTcatcat                                                                                                                                                                            >  2:159809/2‑251 (MQ=255)                                                          ggtccccagctgTTGTGGCCCGCAACGCGGGTGAAATTTTTCTTCTCGGAAATCCCGGAA‑GCCATTTTTGGGAAGAAACCCGTAGAACGGAGCAAAAACGGCTTCCCGGGGCGCGCAGCGTGGATCCA‑GTCCCTGACCGTATTCCCGCGGATGTTTATCCGAAGGCTCGGTTCCAATCCCGTGAAAAATATAATGAATACTATTATTAAAATAACCGCCGCAGCGTCCATGCTTCCGCCGTTATTcatcat                                                                                                                                                                            <  1:583591/243‑1 (MQ=255)                                                           gTTCCCTGCTGATGTGGCCCGCAACGCCGGTGAAAATTTTCTTCCCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACGAAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCACCCCGCTGATGATTATCCCGATGCTCGGTCCCAATACCGTGCAAGATCTGATGAATACTATTATTAATTTGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATg                                                                                                                                                                           <  1:446834/251‑1 (MQ=255)                                                                    tgATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCACGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTAc                                                                                                                                                                  >  1:115541/1‑251 (MQ=255)                                                                              cGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTAc                                                                                                                                                        >  1:300843/1‑251 (MQ=255)                                                                                 aaccccGGTAAAAATTTTCTTC‑CCCAAAACCCGGAAAGGCCTTTTTGGTAAAAAACCCTTCGACCTGACCAAAACCGCCGTCCCGGCGCGCGCAGCGTGGATCCG‑GTTCCTGATCGTCATCCCGCTGATGATTACCCCGATGCTGGGTTCCAATACCTTGCAAAATCTGATGAATACTATTATTAATTTTACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTAcgcg                                                                                                                                                     <  1:21193/247‑1 (MQ=255)                                                                                      cGGTGAAAATTTTCTTCTCCGAAATCACGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCaa                                                                                                                                                 >  1:493830/1‑250 (MQ=255)                                                                                          gAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAAtt                                                                                                                                              >  1:309267/1‑249 (MQ=255)                                                                                          gAAAATTTTATGCCCCAAAATCCAGAAA‑GGCATTTTTGTCAAGAAACCCGCCGAACTGAACAAACGCGGCGTCCGGGCGCTCGTAGCGTG‑ATTCATGTTCCTCTTCGTCATCCCGCTGATGATTATCCTGATGCTCGGTCCCAATCCGGTACAAGTTCTGATAAAAACTATTTTTAATCTTTCCGCCGCCGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTa                                                                                                                                             <  1:153587/250‑1 (MQ=255)                                                                                           aaaaTTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGAGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATACCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAAtt                                                                                                                                              >  2:368078/1‑248 (MQ=255)                                                                                             aaTTTTTTTCCCCGAAATCCCGAAA‑GGCATCTTTGGAAAGAAAACCGCCAAACGGAACGAAACCGGCGTTCCGGCGCGCGCAGCGTGAATCCA‑GTTCCTGATCGTCACCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGAGGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATc                                                                                                                                         <  1:492447/251‑1 (MQ=255)                                                                                                     ttccccgaaaccccgatgggCATCTTGGTTAAGAAACCCGTCGAACTAACCAAAAACGGCGTCCCGGCCGGCGCAGCGTGGATCCA‑GTTCCGGTCCGTCACCCCGCTTATGATTACCCCGATGCTGGTTTCCAATCCCGTGCAAGATCTGACAAATTCTTTTATTAATTTCACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGc                                                                                                                                   <  2:121561/232‑1 (MQ=255)                                                                                                          gAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCAcgcg                                                                                                                             >  1:234999/1‑250 (MQ=255)                                                                                                           aaaTCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGAt                                                                                                                           >  2:477496/1‑251 (MQ=255)                                                                                                            ccgattagAA‑GGCATCTTGGGAAAAAAACCCGTCAAACTGAACGAAAACGGCGTCCCGGCGCGCGCTGCGTGGATCCA‑TTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATCCCGTGCAAGATCTGAGGAATATTATTATAAATAGGTCCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGAtt                                                                                                                          <  2:68443/244‑1 (MQ=255)                                                                                                            aaTCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGAtt                                                                                                                          >  1:642751/1‑251 (MQ=255)                                                                                                                ccGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCg                                                                                                                      >  1:536256/1‑251 (MQ=255)                                                                                                                 gggAA‑GGCATCTTTGGTAAGAAACCCGGCAAACTGAACGAAACCGGCGTTCCGGCGCGCGCAGCGGGGATCAA‑TTCCCTGATCGTCACCCGGCTGATGATTATCCCGATGCTCGTTTCCAATCCCGTCCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGt                                                                                                                     <  2:191256/250‑1 (MQ=255)                                                                                                                   gAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGAtt                                                                                                                          >  1:68443/1‑244 (MQ=255)                                                                                                                   gAA‑GGCATCTTTGGTAAGAAAACCGTCGAAATGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTAt                                                                                                                   >  1:279216/1‑251 (MQ=255)                                                                                                                          aTCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTc                                                                                                             >  1:476131/1‑251 (MQ=255)                                                                                                                          aTCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTc                                                                                                             >  2:513799/1‑251 (MQ=255)                                                                                                                              tggttAAGAAAGCCGTCAAACGGAAGAAAAACGTCGTTCCGCCGCGCGCAGCGGGGATCCG‑TTTCCTGTTCTTAATCCCGCGGATAATTACCCCGATTCTCGGTACCAAAACCGTGCAAACTCTGATGAATACAATTATTAATATAACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTccc                                                                                                           <  1:470917/245‑1 (MQ=255)                                                                                                                                                aaCTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTAGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCgtt                                                                                       >  1:570686/1‑251 (MQ=255)                                                                                                                                                  cTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCgttgt                                                                                     >  1:372325/1‑251 (MQ=255)                                                                                                                                                                 gTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGc                                                                      >  1:249287/1‑251 (MQ=255)                                                                                                                                                                      ggCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGatat                                                                 >  1:134807/1‑251 (MQ=255)                                                                                                                                                                                                       tCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCTCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGACTTATCGTTGTTTCAATGCTGATTGCGATATTTGCCTTAGGGTTTGTCGCTTCGACATTCCCg                                 >  2:450261/1‑251 (MQ=255)                                                                                                                                                                                                             cGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGTAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGgc                           >  1:14122/1‑251 (MQ=255)                                                                                                                                                                                                                              aTGCTCGGTTCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACcat          >  1:426827/1‑251 (MQ=255)                                                                                                                                                                                                                                       tCCAATACCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCAtttttt  >  2:170419/1‑250 (MQ=255)                                                                                                                                                                                                                                             aCCGTGCAAGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTg               >  2:419480/1‑231 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                            CTACCGGAAGCGTTGATGAATCGCTTTGTCGGTCTGGTGTCCTTTACCGCGATGTTCGGTTCCCTGCTGATGTGGACCGCAACGCCGGTGAAAATTTTCTTCTCCGAAATCCCGGAA‑GGCATCTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGCGCAGCGTGGATCCA‑GTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCTGGCTTACCTGAATTTACGCGCCAAATTAGATCACCTGCCACGCGATTTCCGTATGGGCTCCCGCCGCACCGGTATTATCGTTGTTTCAATGCTGATTGCGATATTTGCCGTAGGGTTTGTCGCTTCGACATTCCCGACTGGCGCGAATATTCTGACCATCATTTTTT  >  NC_000913/3227068‑3227545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑