Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,135,448	C→T	Y240Y (TAC→TAT)	flgG →	flagellar basal‑body rod protein FlgG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,135,448	0	C	T	100.0%	230.3 / NA	69	Y240Y (TAC→TAT)	flgG	flagellar basal‑body rod protein FlgG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/37);  total (32/37)

GCGTAACCCAACAAGGCCAGGCAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTACGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGA  >  NC_000913/1135201‑1135693                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      gCGTAACCCAACAAGGCCATGCAGCGCCGGTTCAGGTTTGGCAGCTCAATCTCATCACCTTTATGAATGACACCGGGCTGCAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTTCTGTATCAAGGGTATGTTGAAACGGCTAACGTCAACGTGGCGGAAGACCTGGTCAATATGATTCAGGTGCAACGCGCTTATGaa                                                                                                                                                                                                                                                    <  1:167898/251‑1 (MQ=255)          aacaaGGCCAGGCAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCATCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGt                                                                                                                                                                                                                                           <  1:119759/251‑1 (MQ=255)           acaaGGCCAGGCAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCATCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAAAAGt                                                                                                                                                                                                                                           >  1:25195/1‑250 (MQ=255)              aGGCCAGGCAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCATCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAg                                                                                                                                                                                                                                       <  2:563816/251‑1 (MQ=255)                       aGCTCCGGTTCAGGTTGGGCAGCTCAATCTCATCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTCTGAAATCAACAGTAAAGCGGTGTcc                                                                                                                                                                                                                               >  1:637646/1‑250 (MQ=255)                        gCTCCGGTTCAGGTTGGGCAGCTCAAGCTCATCACCTTTATGAATGACACCGGGCTGGAGATCATTGGCGAAAACCTCTGCTCCTAAACTCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGTTATGTTGAAACGGCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACACGCTTATGAAATCAACAGTAAAGCGGTGTccac                                                                                                                                                                                                                             <  1:408341/251‑1 (MQ=255)                           ccGGTTCAGGTTGGGCAGCTCAATCTCATCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCcaccac                                                                                                                                                                                                                          >  2:573837/1‑251 (MQ=255)                            cgtgtCAGGTTGGGCAGCTCAATCTCATCACCTGTATTAATGACACCGGGCTGGAGAGCATTGGAGAAAACCTCTACACCGAAACGCAATCCTCGGGTTCACCGAACGAAAGCACGCCGGGTCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGGCAACGTGGCGTAAGAACGGGTCAAGATGAGTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCaccacc                                                                                                                                                                                                                         <  2:309059/247‑1 (MQ=255)                              gTTCAGGTTGGGCAGCTCAATCTCATCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTCCACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATTAAATCAACAGTAAAGCGGTGTccaccccc                                                                                                                                                                                                                         <  1:227785/249‑4 (MQ=255)                                          cAGCTCAATCTCATCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCa                                                                                                                                                                                                           <  1:95974/251‑1 (MQ=255)                                             cTCAATCTCATCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGaaaa                                                                                                                                                                                                         >  2:227563/1‑250 (MQ=255)                                               cAATCTCATCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAAc                                                                                                                                                                                                      >  1:144504/1‑251 (MQ=255)                                               cAATCTAATCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGAGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCaaaaa                                                                                                                                                                                                       >  2:95210/1‑250 (MQ=255)                                                 aTCTCATCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGAGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTg                                                                                                                                                                                                    >  1:501547/1‑251 (MQ=255)                                                      atcaccTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCa                                                                                                                                                                                               <  1:98240/249‑1 (MQ=255)                                                         cccTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAAct                                                                                                                                                                                            <  2:348111/250‑1 (MQ=255)                                                               atgaatgaCACCGGGCTGGAGAGCATTGGCAAAAACCTCTACACCGAAACTCAATCCTCTGGTGCCCCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAGCGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCTGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAgg                                                                                                                                                                                      <  1:509235/251‑1 (MQ=255)                                                                   atgaCACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGTGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTa                                                                                                                                                                                  <  2:261285/251‑1 (MQ=255)                                                                                ggAGAGCATTGTCGAAAGCCGCTACTCCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGTCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGTTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAg                                                                                                                                                                       <  1:95210/249‑1 (MQ=255)                                                                                    agCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCAc                                                                                                                                                                 >  1:329621/1‑251 (MQ=255)                                                                                    agCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCAc                                                                                                                                                                 >  1:142317/1‑251 (MQ=255)                                                                                      cATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCTCCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCg                                                                                                                                                               <  1:300303/251‑1 (MQ=255)                                                                                         tGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGtt                                                                                                                                                            >  1:7712/1‑251 (MQ=255)                                                                                         tGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGt                                                                                                                                                             <  1:579403/250‑1 (MQ=255)                                                                                          ggCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGAAGGTTCACCGGttt                                                                                                                                                           >  1:636648/1‑251 (MQ=255)                                                                                                acccTCTACGCCGAAACGCAAGCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTTTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCACCGCGCTTATTAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGATTAACCGGTGGCAGGTTCACCGGTTTACTGAt                                                                                                                                                     <  2:612213/249‑1 (MQ=255)                                                                                                aaCCTATACACCGAAGCGGAAGCCGCTGGGGCACCGTACAAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCGGTATCAAGGTTATGTTGTAACGTCTAACGTTAACGTGGCGGAACAACTGGTCAATATGGTTCAGGAGCAACGCGCTTATGAAATCAAGAGTAAATCGGGGTCCAGCGCCGATCAGAGGCTGCGAAAACTGACGTGTCTCTAAGGCGTAACCGGTGGCAGGTGCACCGTTTTACTGAt                                                                                                                                                     <  2:264608/251‑1 (MQ=255)                                                                                                  ccTCTACACCGAAACGCAATCCGCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGGCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCATATGCTGCAAAAACTGACGCATCTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGAttt                                                                                                                                                   <  2:209317/251‑1 (MQ=255)                                                                                                      tACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTg                                                                                                                                                >  1:513494/1‑250 (MQ=255)                                                                                                       acacCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTg                                                                                                                                                <  1:400303/249‑1 (MQ=255)                                                                                                        cacCGACACGCAATCCTCTGGAGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCCACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGAAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGa                                                                                                                                               >  2:276480/1‑249 (MQ=255)                                                                                                        cacCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGa                                                                                                                                               >  1:33370/1‑249 (MQ=255)                                                                                                              aaCGCAATCCTCTGGGGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGACAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAgatgat                                                                                                                                        >  2:206208/1‑250 (MQ=255)                                                                                                                 gCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTg                                                                                                                                                                           >  1:574692/1‑212 (MQ=255)                                                                                                                 gCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTg                                                                                                                                                                           <  2:574692/212‑1 (MQ=255)                                                                                                                                aCCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCt                                                                                                                     >  1:399737/1‑251 (MQ=255)                                                                                                                                       gaaAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTAACAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCTCCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTTGCAGGTTCACCGGTTTACTGATTTTTGAAGATGCTAGCCATGCAAAAAAACGCTGCGCATa                                                                                                              <  2:470312/251‑1 (MQ=255)                                                                                                                                        aaaGCACGCCGGGCCTGAACGGCGCGGGTCTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATAc                                                                                                             <  1:582429/251‑1 (MQ=255)                                                                                                                                              cGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGc                                                                                                       <  2:7712/251‑1 (MQ=255)                                                                                                                                              cGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGc                                                                                                       >  1:41727/1‑251 (MQ=255)                                                                                                                                              cGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGc                                                                                                       >  1:405080/1‑251 (MQ=255)                                                                                                                                                  gggCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCAtt                                                                                                   >  2:512908/1‑251 (MQ=255)                                                                                                                                                        gAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAg                                                                                              <  2:563631/250‑1 (MQ=255)                                                                                                                                                        gAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGc                                                                                             <  1:39862/251‑1 (MQ=255)                                                                                                                                                         aaCGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCt                                                                                            <  1:386177/251‑1 (MQ=255)                                                                                                                                                                          gTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTa                                                                           <  2:396114/251‑1 (MQ=255)                                                                                                                                                                           tATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGCTTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTa                                                                           >  1:423426/1‑250 (MQ=255)                                                                                                                                                                              cAAGGGTATGTTGAAACGGCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCg                                                                       <  2:637646/251‑1 (MQ=255)                                                                                                                                                                                    tATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTgcg                                                                 >  1:127300/1‑251 (MQ=255)                                                                                                                                                                                      tGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTATGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGcc                                                               >  1:179009/1‑251 (MQ=255)                                                                                                                                                                                          gAAACGGCTAACGTCAAAGAGGCGGGAGCACTGGTAAATATGATGCACGGGTAGCGAGGTTATGAAATCAAAAGAAAAGCGGGGTCCACCACCGATCAGATGCCGCAGAAACTGACGCAACTCTAAGGCTAAACAGGAGGCAGGTTCACCGCATTACTGATATATGAAGATGATAGCCATGCAAAAAAACGCTGCTCATAATTATGCCATGTCCAGCTTGTTGGAGCTTTCACGAACAGGCTGCGCCTggg                                                           >  2:153325/1‑250 (MQ=255)                                                                                                                                                                                           aaaCGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGAt                                                          <  2:455896/251‑1 (MQ=255)                                                                                                                                                                                                 cTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTACCCGGCTGCGCCTGGATACCCTc                                                    >  2:622594/1‑251 (MQ=255)                                                                                                                                                                                                     cGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCAcg                                                >  2:649300/1‑251 (MQ=255)                                                                                                                                                                                                        cAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCAcgcc                                              <  1:315194/250‑1 (MQ=255)                                                                                                                                                                                                         aaCGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCAcgccgc                                            >  2:193599/1‑251 (MQ=255)                                                                                                                                                                                                             tGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGt                                        >  1:508731/1‑251 (MQ=255)                                                                                                                                                                                                             tGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTATAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACAGGCTGCGCCTGGATACCCTCCAcgccgtcggg                                        >  2:148288/1‑246 (MQ=255)                                                                                                                                                                                                              gggggAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAAAAGAAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCATGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGGGCCTGGATACCCCCCACGCCGCTGGt                                        <  2:18318/247‑1 (MQ=255)                                                                                                                                                                                                                          ggtgaatctGATTCAGGTGCAACCCTCATATGAAACCAACAGTAAAGCGGAGTCCACCACCGATCAGATGCTGCAAAAACTGACTCAACTCTAAGGCTTAACCGGTGGCAGCTTCACCGGTTTACTGATTTTTGAAGATGAGAACCTTCAAAAAAAACGCTGCGAATCCTTATGCCATTTCCACCTTGTTGGTGTTGTCGCTAACCGGCTGCCCCTGGATCCCCTCCACGCCGCTGGTGCAGGGGGCGAcc                           <  1:460046/243‑1 (MQ=255)                                                                                                                                                                                                                           gTCAATATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGAcc                           >  1:498384/1‑250 (MQ=255)                                                                                                                                                                                                                              aaTATGATTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGCTGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTg                       >  2:660523/1‑251 (MQ=255)                                                                                                                                                                                                                                    aTTCAGGTGCAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAACCTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAACCGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAAc                 <  1:660523/251‑1 (MQ=255)                                                                                                                                                                                                                                             cacngcgcTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAATGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATCCCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCg        <  2:585962/247‑1 (MQ=255)                                                                                                                                                                                                                                             cAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCg        <  2:41727/251‑1 (MQ=255)                                                                                                                                                                                                                                             cAACGCGCTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCg        >  1:102788/1‑251 (MQ=255)                                                                                                                                                                                                                                              aaCGCGGTTATGAAATCATCAGTAAAGCGTTGTCTACCGCAGAGCAGATGCTGAAAAAACTGATGCGACTATAGGGCTTAACCGGTGCCAGATGCACCGGTTTACTGATTTTGGAAGATGATAGCCATGCAAAAATCCGCTCGGCATACTTAGGCCATTTCCAGCTGTTTGTTGCTTTCACTAACCGGCTGCGCCTTGATACCCTCCACGCCGCTGGTGCAGGGGGCTACCAGTGCTTAACCGGTTctcgt       <  2:426135/251‑5 (MQ=255)                                                                                                                                                                                                                                                 gcgcTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTcc    <  1:512908/251‑1 (MQ=255)                                                                                                                                                                                                                                                   ccTTATGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGa  <  2:508731/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      GCGTAACCCAACAAGGCCAGGCAGCTCCGGTTCAGGTTGGGCAGCTCAATCTCACCACCTTTATGAATGACACCGGGCTGGAGAGCATTGGCGAAAACCTCTACACCGAAACGCAATCCTCTGGTGCACCGAACGAAAGCACGCCGGGCCTGAACGGCGCGGGACTGCTGTATCAAGGGTATGTTGAAACGTCTAACGTCAACGTGGCGGAAGAACTGGTCAATATGATTCAGGTGCAACGCGCTTACGAAATCAACAGTAAAGCGGTGTCCACCACCGATCAGATGCTGCAAAAACTGACGCAACTCTAAGGCTTAACCGGTGGCAGGTTCACCGGTTTACTGATTTTTGAAGATGATAGCCATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACCGGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTTCCCGGTCCGA  >  NC_000913/1135201‑1135693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑