Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,025,706	G→A	Q452Q (CAG→CAA)	xanQ →	xanthine:H(+) symporter XanQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,025,706	0	G	A	100.0%	99.4 / NA	34	Q452Q (CAG→CAA)	xanQ	xanthine:H(+) symporter XanQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/16);  total (18/16)

ACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTCATTAAACAGGGAAAAGTGGAATGGTTTGGCGAATGGGAAAACGGAAAGCATCAAATTCCTG  >  NC_000913/3025467‑3025949                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    aCGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGTTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGGTAACTGCGATTTTATTTAATATTATCCTCCCCGG‑TGGCTACCGACAAGaaaaaggtct                                                                                                                                                                                                                                          >  1:290839/1‑245 (MQ=255)    ttGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTTACTGCGGTTTTTTTAAATATTATCCTCCCCGG‑TGGCTACCGGCAAGAAAAAGTTCttcc                                                                                                                                                                                                                                       >  1:45484/1‑248 (MQ=255)                 tgcttGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTTACTGCGATTTTATTTTATATTTTCCTCCCTGG‑GGGCTACCGACAAAAAAACggttttcctggtttttactcc                                                                                                                                                                                                                          >  2:343025/4‑231 (MQ=255)                  gATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAg                                                                                                                                                                                                                         <  1:644382/251‑1 (MQ=255)                        cATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGCTTTTATTATATATTATCCTCCCTCG‑TGGCGACCGACAAGAAAACGGTCTGGCTGGTATTTCCTCAGCggg                                                                                                                                                                                                                     >  2:465200/1‑248 (MQ=255)                              aGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTTTTTAATATTATTCTCCCCGG‑TGGCTACCGACCAGAAAACGTTCTGCCTGGTATTACCTCAGCGGCAGGGATgg                                                                                                                                                                                                             >  1:241692/1‑251 (MQ=255)                                           atcatcaCCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTCTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAg                                                                                                                                                                                                <  2:440894/251‑1 (MQ=255)                                              atcaCCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAg                                                                                                                                                                                             <  2:18854/251‑1 (MQ=255)                                                          ttNAAGCGCCGTGAAACGCTTAGTGTCGCCCCTTATTTAGGTTTAGGGCTTGGCGTTTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCGATTTATGTAGTAGTTGAAAACCCTATTTGTGCTGGTGGGTTAACGGCGATTTTAATAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTGCCTCAGCGGAAGAGATGGATTAGCAGTAAATGAGTCAAGGATGTc                                                                                                                                                                                  <  2:603267/250‑1 (MQ=255)                                                           tAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCa                                                                                                                                                                                 <  2:330549/250‑1 (MQ=255)                                                             aaGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGACTTTATTAAATATTATTCTCCCCGG‑TGGCTACCGACCAGAAAACATTATGCCCGGAATTACCTCAGCGGAAAAGGTGGATTAAAAGTCAAGGaaggaatgaatgcaggg                                                                                                                                                                              >  1:608030/1‑235 (MQ=255)                                                              aGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAgg                                                                                                                                                                               >  1:17338/1‑249 (MQ=255)                                                                       aaaCACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAAATGCGCTTTTTTTAAATATTTTCCTCCCTTG‑TGGCTACCGACAAGAAAAAGTTCTGCCTGGGCTTAACTCCGCGGGAGAGATGGATTAACAGTAAAGGAGTCAATGATTTCAGGAGAacacncat                                                                                                                                                                    >  2:590414/1‑249 (MQ=255)                                                                                  gTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGt                                                                                                                                                          <  1:284056/250‑1 (MQ=255)                                                                                          ttcttTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTTTTAAATATTATCCTCCCTGG‑TGGGTACCGACAAGAAAAAGTTCTGCCTGGGATTTCCTCAGCGGAAGAGATGGATTAAAAGGAAAGGAGTCAATGATGGCAGga                                                                                                                                                                              >  1:517030/1‑222 (MQ=255)                                                                                          ttcttTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGga                                                                                                                                                                              <  2:517030/222‑1 (MQ=255)                                                                                                                  ctcCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACACTATTTGTGCTGGCGGGTTTAATACCATTTTTTTAAAAATTTTTCTCCCTGGGTGG‑TACCGACAAGAAAAAGTTTTGCCTGGAATTACCTCAACGGAAGAGATGGAGTAAAAGTAAAGGAGTCAATGATGTTAGGAGAAAACACGTTAAAAACGGTACGAGGCAGATTTATTGATGTCACCCGTAcact                                                                                                                         >  2:47531/1‑248 (MQ=255)                                                                                                                    ccTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTAcgatcg                                                                                                                       <  1:450974/251‑1 (MQ=255)                                                                                                                     cTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATACTCCCCGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGCGATGGATTAACAGTAAAGGGGTCAATGATGTCAGGAGAACACCCGTTCAAAACGGTACGAGGACGTTTTTTTGATGTCACCCCTAcgctcg                                                                                                                       >  1:632441/1‑250 (MQ=255)                                                                                                                                                   aGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAA‑GCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTTCCTCTGCTCTGCgg                                                                                       <  1:170886/251‑1 (MQ=255)                                                                                                                                                          aTTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTTTTAAAAATTATCCTCCCTGG‑GGGGTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGGAGAGATGGATTAACAGTAAAGGAGGAAATGATGTCAGGAGAACACACGTTTAAAGCGGTACGAGGGCGTTTTATTGATGGCACCCGGCCGATCGATAACCCGGGAGAGATTGCCTCTGCGCCGCGGtttttt                                                                                 >  2:380166/1‑248 (MQ=255)                                                                                                                                                          aTTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTGCCTCTGCGCTGCGGttt                                                                                    <  2:44277/248‑1 (MQ=255)                                                                                                                                                                 tATTAGTTGAAAACCCTATTTGTGCTGGCGGGGTAACTGCGATTTTATTAAATATTTTCCTCCCTGG‑TGGCTACCGGCAAGAAAACGTTTTGCCTGGTATTACCTCAGCGGAAGAGATGGGTTAACAGTAAAGGAGTCAATGATGTCCGGAGAAAAAACGTTAAAAACGGTACGAGGCAGTTTTATTGATGTCACCCGGACGATCGATAACCCGGAAGAGAATGCCTCTGCGCTGCGGTTTTTTGAGGATg                                                                          >  2:311924/1‑251 (MQ=255)                                                                                                                                                                     aGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTGCCTCTGCGCTGCGGTTTATTGAGGATGGttt                                                                      <  2:339919/251‑1 (MQ=255)                                                                                                                                                                          aaaaCCCTATTTGTGCTGGCGGGGTAACTGCGGTTTTATTAAATATTATCCTCCCCGG‑GGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCCCCGCGGGAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGGGAAAAAACGTTAAAAGCGGTAAGAGGAAGTTTTATTGATGTCACCCGTACGGTCGATAAACCGGAAGAGAATGCCTCTGCGCTGCGGTTTATTGTGGGTGGGttatttt                                                                 >  1:125009/1‑249 (MQ=255)                                                                                                                                                                            aaCCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTc                                                               <  1:380166/251‑1 (MQ=255)                                                                                                                                                                                  aTTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTCATTaa                                                          <  2:45484/250‑1 (MQ=255)                                                                                                                                                                                         cTGGCGGGATAACTGCGATCTTATTAAATATTATCCTCCCTGG‑CGGCTACCGACAAGAAACCGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTCATTAAACAGGGa                                                   >  1:226716/1‑250 (MQ=255)                                                                                                                                                                                                          aTTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGCTGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTCATTAAACAGGGAAAAGTGGAATGGTTTGGc                                 >  1:406834/1‑251 (MQ=255)                                                                                                                                                                                                                       ttatCCTCCCTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTCATTAAACAGGGAAAAGTGGAATGGTTTGGCGAATGGGAAAACg                    >  1:466938/1‑251 (MQ=255)                                                                                                                                                                                                                              cccTGG‑TGGCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTCATTAAACAGGGAAAAGTGGAATGGTTTGGCGAATGGGAAAACGGAAAg               <  2:257202/249‑1 (MQ=255)                                                                                                                                                                                                                                      ggCTACCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTCATTAAACAGGGAAAAGTGGAATGGTTTGGCGAATGGGAAAACGGAAAGCATCAAAtt      <  1:594582/251‑1 (MQ=255)                                                                                                                                                                                                                                          aCCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTCATTAAACAGGGAAAAGTGGAATGGTTTGGCGAATGGGAAAACGGAAAGCATCAAATTCCTg  >  1:336702/1‑251 (MQ=255)                                                                                                                                                                                                                                          aCCGACAAGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGGCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTCATTAAACAGGGAAAAGTGGAATGGGTTGGCGAATGGGAAAACGGAAAGCATCAAATTCCt   <  1:465200/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    ACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGG‑TGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAGATTGCCTCTGCGCTGCGGTTTATTGAGGATGGTTTATTACTCATTAAACAGGGAAAAGTGGAATGGTTTGGCGAATGGGAAAACGGAAAGCATCAAATTCCTG  >  NC_000913/3025467‑3025949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑