Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,372,474	G→A	E484K (GAA→AAA)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,372,474	0	G	A	100.0%	247.9 / NA	73	E484K (GAA→AAA)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/45);  total (28/45)

CGACCCGTGCTGCGGTAGAAGAAGGCGTGGTTGCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACGTGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCA  >  NC_000913/4372229‑4372710                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             cgACCCGTGCTGCGGTAGAAGAAGGCGTGGTTGCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATAc                                                                                                                                                                                                                                         <  1:322978/251‑1 (MQ=255)    cccGTGCTGCGGTAGAAGAAGGCGTGGTTGCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGc                                                                                                                                                                                                                                      >  1:11898/1‑251 (MQ=255)       gTGCTGCGGTAGAAGAAGGCGTGGTTGCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAAc                                                                                                                                                                                                                                   >  1:216785/1‑251 (MQ=255)         gctgcGGTAGAAGAAGGCGTGGTTGCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTTCGGGCAATGGAAGCGCCGTTGCGTCAGTTCGTATTGAACGGTGGCGAAGAACCGTCTGTTGTGGCTAACACCGTTAAAGTCTGCGACGGCAACTAGGGTTACAACTCAGCAACCGAAAAATACGGCAACAt                                                                                                                                                                                                                                 <  2:40873/251‑1 (MQ=255)            gcGGTAGAAGAAGGCGTGGTTGCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGAt                                                                                                                                                                                                                              <  2:377867/251‑1 (MQ=255)                 agaagaAGTCGTGGTTGCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCAGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGGTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACa                                                                                                                                                                                                                         <  2:491832/251‑1 (MQ=255)                 agaagaAGGCGTGGTTGCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATAGAc                                                                                                                                                                                                                          >  2:410686/1‑250 (MQ=255)                    agaagGCGGGGTTGCTGGTAGTGGTGTGGCGATGATCCTCGGAGCGTCTAAACTGGCTGGACTGCGTGGTCAGAACGAAGACCAGAACATGGATATCAAAGTTGCACTGCGTACAATTGAAGCGCCGCTGCGTCAGATCGTATTGAACTGCGGAGAAGAACCGTCTGTTGTTGCTAACCCCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATgg                                                                                                                                                                                                                      <  2:417875/251‑1 (MQ=255)                     gaagGCGTGGTTGCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGAAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGAGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATggg                                                                                                                                                                                                                     >  2:219201/1‑251 (MQ=255)                       agGCGTGGTTGCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGGGGTCAGAACGAAGACCAGAACATGGGGATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAGCCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTa                                                                                                                                                                                                                   <  2:194118/251‑1 (MQ=255)                           gTGGTTGATGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGCACGAAGACCAGAACATGGGTCTCAAAGTTGCACTGCGTGCAATGCAAGCTCCGCTGCGTCAGCTCGAATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACAACGTTAAAGGCGGCGCCGGCACCTACGGTTACATCGCAGCAACCGCAAAATACGGCAACATGATCGACATGGGAATCCt                                                                                                                                                                                                               >  2:368307/1‑251 (MQ=255)                             ggTTGCTGGGTGTGGGGATGCGCGGATCCGCAGAGCGTGGAAACTTGTTGACCTGCGTGGTCAGAACGAAGACCATAACAGGGGTATCAAAGTTTCTCTGCTTGAAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGAGGCGAAGTACCGTCTTTTGTTTCTAACACCGTTAAAGGCGTCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACCGCAACATGATCTACATGGGTCTCCTgg                                                                                                                                                                                                             <  2:650128/251‑1 (MQ=255)                               gtGCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGATGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGAt                                                                                                                                                                                                           <  2:378240/250‑1 (MQ=255)                                 gCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGTGTATCCGGGATc                                                                                                                                                                                                          <  2:298102/250‑1 (MQ=255)                                 gCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGAACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAACGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATcc                                                                                                                                                                                                         >  2:223906/1‑251 (MQ=255)                                     gtggtggtgTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATccaacc                                                                                                                                                                                                     >  1:514176/1‑251 (MQ=255)                                          ggtgTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTCTCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGCAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGCTCCCACCAAAGt                                                                                                                                                                                                >  1:610167/1‑251 (MQ=255)                                             gtTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCATAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTatc                                                                                                                                                                                             <  2:434477/251‑3 (MQ=255)                                                  gcTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTC‑‑TTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTtct                                                                                                                                                                                      <  1:55231/251‑1 (MQ=255)                                                     gATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTtct                                                                                                                                                                                      <  1:384735/250‑1 (MQ=255)                                                      aTCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTtctgc                                                                                                                                                                                    >  1:431251/1‑251 (MQ=255)                                                             tAGCGTCTAAACTGGCTGAACTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATACAACCAAAGTAACTCGTtctgctctgc                                                                                                                                                                               >  2:567166/1‑249 (MQ=255)                                                              aGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAGCTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAg                                                                                                                                                                             <  2:212616/250‑1 (MQ=255)                                                                 gTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACg                                                                                                                                                                         >  1:470109/1‑251 (MQ=255)                                                                   cTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGc                                                                                                                                                                        >  1:520743/1‑250 (MQ=255)                                                                     aaaCTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCa                                                                                                                                                                       <  2:209929/249‑1 (MQ=255)                                                                     aaaCTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACAGGGGTATCAAAGTTGCACTGCGTGCAATCGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCGAACTCCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGGAGCAACCGAAAAATGCGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAATTCGTTCTGCTCTGCAGTACGCa                                                                                                                                                                       <  2:67961/249‑1 (MQ=255)                                                                          ggCTGACCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTc                                                                                                                                                                  <  2:492748/249‑1 (MQ=255)                                                                               aCCTGCGTGGTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGtggct                                                                                                                                                           <  2:216785/251‑1 (MQ=255)                                                                                        gTCAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCTGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGTCAACTACGGTTACAACGCAGCAACCGAAAAACACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCtgatg                                                                                                                                                  <  1:519077/251‑1 (MQ=255)                                                                                          cAGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGGGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGTGTATCCTGGATCCAATCAAAGTAACTCGTTCTGCTCTGCAGTTCGCAGCTTCTGTGGCGGGCCtgatga                                                                                                                                                 <  2:88274/250‑1 (MQ=255)                                                                                          cAGAACAAATACCAGAACATGGGTATCAAAGTTGCACTTCGTGCAATGGAAACTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAATCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCttatga                                                                                                                                                 <  2:640186/250‑1 (MQ=255)                                                                                           aGAACGAAGACCAGAACATGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATc                                                                                                                                               >  2:442674/1‑251 (MQ=255)                                                                                                      cAGAACATGGGTATCACAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGCTCACCACCGAATg                                                                                                                                    >  1:209564/1‑251 (MQ=255)                                                                                                         aacatGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCAt                                                                                                                                 <  2:11898/247‑1 (MQ=255)                                                                                                              gggTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGtt                                                                                                                             <  2:203706/250‑1 (MQ=255)                                                                                                               ggTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTAc                                                                                                                           <  2:267028/251‑1 (MQ=255)                                                                                                                gTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTAcc                                                                                                                          >  1:268295/1‑251 (MQ=255)                                                                                                                    cAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGAc                                                                                                                       <  2:511953/250‑1 (MQ=255)                                                                                                                      aaGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTg                                                                                                                    >  1:456974/1‑251 (MQ=255)                                                                                                                         ttGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCtt                                                                                                                                                                   >  2:111212/1‑201 (MQ=255)                                                                                                                         ttGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCtt                                                                                                                                                                   <  1:111212/201‑1 (MQ=255)                                                                                                                          ttcACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCg                                                                                                                 <  2:514176/248‑1 (MQ=255)                                                                                                                                    gCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATg                                                                                                       <  1:289/250‑1 (MQ=255)                                                                                                                                         ggAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTGACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCt                                                                                                  <  1:385822/250‑1 (MQ=255)                                                                                                                                              cgccGCTGCGTCAGATCGTATTGAACTGCGGCGAAGCACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCTACGGCAACTACTGTTACAACGCAGCAACCGAAAAATACGGCAACATTATCGTCATGGGTATCCTGGTTCCAACCAAAGTAAGTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCTCCGAATGCATGGTTACCGTCCTGCCGAAAAACGATTCAGCTGACTTa                                                                                            <  2:211245/249‑1 (MQ=255)                                                                                                                                                       gTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCgctgct                                                                                   >  2:163235/1‑251 (MQ=255)                                                                                                                                                           gATCGTATTGAAATGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTCTCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCCCCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCg                                                                               >  2:385667/1‑251 (MQ=255)                                                                                                                                                               gTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGGTAAAGGCGGCGA‑GGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATg                                                                          <  2:514921/251‑1 (MQ=255)                                                                                                                                                                 aTTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATgg                                                                         >  1:584766/1‑251 (MQ=255)                                                                                                                                                                     aaCTACGGCGAAGAACCGCCTGTTGCTGCTAACACCGTTAAAGGCGGCGACGGCAACCACGGTTACAACGCAGCAACCGAAAAATACGGCAACACGATCGACATGGGTATCATGGATCCAACCAAAGTAACTAGTTCTGCTCTGCAGTACGCAGCTTCTGCGGCTGGCCTGATGAACACCACCGAATGCATGGGTACGGACCTGCCGAAAAACGACGGAGCCGACTTAAGTGCTGCTGGCGGTATgggg                                                                       >  2:433051/1‑248 (MQ=255)                                                                                                                                                                         gcggcgAAGAACCGTCTTTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCATTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATTCATGGTTACCGACCTGCCGAAAAACGATGCATCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATg                                                                 <  2:661185/251‑1 (MQ=255)                                                                                                                                                                         gcggcgAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTaa                                                                                                                                                                                              <  1:94920/126‑1 (MQ=255)                                                                                                                                                                         gcggcgAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTaa                                                                                                                                                                                              >  2:94920/1‑126 (MQ=255)                                                                                                                                                                            gcgAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGt                                                              <  2:243004/251‑1 (MQ=255)                                                                                                                                                                             cgAAGAACCGTCTGTTGTTGCGAACACCGTTAAAGGCGGCGCCGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTg                                                             <  2:14496/251‑1 (MQ=255)                                                                                                                                                                              gaagaaCCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTgg                                                            <  2:499937/251‑1 (MQ=255)                                                                                                                                                                                    ccGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATggg                                                      <  1:442674/251‑1 (MQ=255)                                                                                                                                                                                     cGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGggc                                                     <  1:619319/251‑1 (MQ=255)                                                                                                                                                                                      gTCTGTTGCTGCTAACACCGTTAAAGGCGCCGACGGAAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACCTGATCGACATGGGCATCATGGATCCATCCAAAGGAACTCGTTCTGCTCTGCAGTACGCAGCTCCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGGATGGGCGGCCTGGGTGGGCTGggcg                                                    >  2:47785/1‑251 (MQ=255)                                                                                                                                                                                      atCTGTTGTAGCTAACAACGTTAAAGGCGGCGACGACAACTACGGTTACAACGCAGCAACAGAAAAATATGGAAACATGATCGACATGGGTATCATGGATCCAAACAAAGTAACACGTTATGCTATGCAGTACGCAGATTATGTGGCTGGACTGATGATCACCACAGAACGCATGGTTACCGACCTGCCGAAAAACGATGAAGCTGCATTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGggcg                                                    >  2:22500/2‑251 (MQ=255)                                                                                                                                                                                       tCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGggcgg                                                   >  1:424091/1‑251 (MQ=255)                                                                                                                                                                                                gCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTa                                          <  2:184450/251‑1 (MQ=255)                                                                                                                                                                                                    acacCGTTAAAGGAGGCCACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGCTATCCCGGATCCAACCAACGCAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAACAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATCGGCGGCATGGGCGGCATCGGCGGCATGGTGTAATTg                                      >  2:273549/1‑251 (MQ=255)                                                                                                                                                                                                                  ggcgACGGCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGc                         >  1:170964/1‑250 (MQ=255)                                                                                                                                                                                                                       cGGCAACTACGGTTACAACTCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGGTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACGTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAAt                   <  1:385667/251‑1 (MQ=255)                                                                                                                                                                                                                        ggCAACTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATa                  <  2:140771/251‑1 (MQ=255)                                                                                                                                                                                                                             cTACGGTTACAACGCAGCAACCGAAAAATACGTCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACGCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGGCCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGTCATGATGTAATTGCCCTGCACCTCg                          <  2:416310/238‑1 (MQ=255)                                                                                                                                                                                                                             cTACGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCg                          >  1:416310/1‑238 (MQ=255)                                                                                                                                                                                                                                cGGTTACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAATCAAAcc          <  1:199608/251‑1 (MQ=255)                                                                                                                                                                                                                                    tACAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAAccccc       <  2:171037/250‑1 (MQ=255)                                                                                                                                                                                                                                      cAACGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCggg    <  2:456974/251‑1 (MQ=255)                                                                                                                                                                                                                                        aCGCAGCAACCGAAAAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCa  <  1:410686/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             CGACCCGTGCTGCGGTAGAAGAAGGCGTGGTTGCTGGTGGTGGTGTTGCGCTGATCCGCGTAGCGTCTAAACTGGCTGACCTGCGTGGTCAGAACGAAGACCAGAACGTGGGTATCAAAGTTGCACTGCGTGCAATGGAAGCTCCGCTGCGTCAGATCGTATTGAACTGCGGCGAAGAACCGTCTGTTGTTGCTAACACCGTTAAAGGCGGCGACGGCAACTACGGTTACAACGCAGCAACCGAAGAATACGGCAACATGATCGACATGGGTATCCTGGATCCAACCAAAGTAACTCGTTCTGCTCTGCAGTACGCAGCTTCTGTGGCTGGCCTGATGATCACCACCGAATGCATGGTTACCGACCTGCCGAAAAACGATGCAGCTGACTTAGGCGCTGCTGGCGGTATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAATTGCCCTGCACCTCGCAGAAATAAACAAACCCCCGGGCA  >  NC_000913/4372229‑4372710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑