BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,023,453	C→T	S713F (TCC→TTC)	xdhD →	fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,023,453	0	C	T	100.0%	362.1 / NA	103	S713F (TCC→TTC)	xdhD	fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (52/51); total (52/51)

Only 100 of 103 total aligned reads displayed.

ATCCAATGGTCGTCACCGAAACCGCAAAATGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTCCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTGG  >  NC_000913/3023209‑3023699

atCCAATGGTCGTCACCGAAACCGCAAAATGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTg                                                                                                                                                                                                                                                    >  1:125548/1‑250 (MQ=255)

  ccaagagtcggcACCGAAACCGCAAAATGGTGACTGGCACATCGGTCGCGGCGACGCCATTATCAGGAAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCAGGAGCAAACTGTAAACGGACGGCACCTTTATCGTTCATTCTGGCGGGTTGGATATTGGTACTGTTCTTGATACTGTAGTGACTAAACTGGCAGCACAA‑TTGCTGCACTGCACACCGCAGGACGTGCATGTTATCTTCGGTGAt                                                                                                                                                                                                                                                  <  2:359641/244‑1 (MQ=255)

        gtcgtcACCGAAACCGCAAAATGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATc                                                                                                                                                                                                                                           <  1:304661/251‑1 (MQ=255)

          cgtcACCGAAACCGCAAAATGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCAt                                                                                                                                                                                                                                         >  1:279825/1‑251 (MQ=255)

           gtcACCGAAACCGCAAAATGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATg                                                                                                                                                                                                                                        >  1:562452/1‑251 (MQ=255)

           gtcACCGAAACCGCAAAATGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATg                                                                                                                                                                                                                                        <  1:92745/251‑1 (MQ=255)

             cACCGAAACCGCAAAATGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGTCGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGc                                                                                                                                                                                                                                       <  2:376286/250‑1 (MQ=255)

               cctaacccccAAAAGGGTGTCTGGCACATCAGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGAGGAGGCTAACGTG‑ATGAACAAACTGGACTCGGACGGCTCCTTTCTGGTTTAGTCTGCCGGTGCTGATATTGGTACTGGTCTGCATACTGTAGGGACGAAGCTAGCAGAAGAA‑GTGCTTCTCTGCCCACCGCAGGACGTGCGTGTTATATTCGGTGATattgaacaagcg                                                                                                                                                                                                                                      <  2:329960/240‑12 (MQ=255)

               ccGAAACCGCAAAATGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGCAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGTCGTGCATGTTATCTTCGGTGATACCGATCATGCgtt                                                                                                                                                                                                                                    >  1:382117/1‑251 (MQ=255)

               ccGAAACCGCAAAATGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCgtt                                                                                                                                                                                                                                    >  1:392884/1‑251 (MQ=255)

               ccGAAACCGCAAAATGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCgtt                                                                                                                                                                                                                                    >  1:60686/1‑251 (MQ=255)

                  aaaCCGCAAAATGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCGCCGCAGGACGTGCATGTTATCTTCGGTGAGACCGATCATGCgttgtt                                                                                                                                                                                                                                 <  2:419658/251‑1 (MQ=255)

                      cGCAAAATGGTGGCTGGCACATCGGTCGCGCCGCCGCATTTATCATGCAGAAATCGGGTATCCCGGATCTCGATCAGGCTAAC‑TGTATGTTCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTTGCGGTGCTGATATTGGTACTGGTCTGGTTACTGTAGTGACGAAACTGGACGCAAAA‑GTGCAGCACTGCCCACAGCAGGACGTGCATGTTATCTTCGTTGATACTTCGCATGCGTTGTTTGAt                                                                                                                                                                                                                             >  2:177573/1‑251 (MQ=255)

                      cGCAAAATGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGAt                                                                                                                                                                                                                             >  1:132618/1‑251 (MQ=255)

                            aTGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGgc                                                                                                                                                                                                                       <  1:393374/251‑1 (MQ=255)

                                 gACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCTCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTGTGATAAAGGCGCat                                                                                                                                                                                                                   <  2:237737/250‑1 (MQ=255)

                                     ggCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGcc                                                                                                                                                                                                              >  1:551293/1‑251 (MQ=255)

                                       cacaTCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTc                                                                                                                                                                                                            <  2:392884/251‑1 (MQ=255)

                                        acaNCGGTCGCGGCGTCGCCATTATCATGCAGAAATTGGGTATCCCGGATAGCGATCAGGCTAAC‑TGCATGAGCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTTACGAAACTGTCCGCAGAA‑GTGCTGCCCTGCCCACCGCAGGACGTGCATTTTATCTTCGGTGATACCTATCATGCGTTGTTTGATAAAGGCGCATATGCCTCg                                                                                                                                                                                                           <  2:585533/251‑1 (MQ=255)

                                            cGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATTATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATAGTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTTCCCACCGCAGGACGTGCATGGTATCTTCGGTGATACCGATCAGGCGTTGTTTGATAAAGGCGCATATGCCTCGTcc                                                                                                                                                                                                        <  1:651912/250‑1 (MQ=255)

                                            cGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTcc                                                                                                                                                                                                        <  1:127130/250‑1 (MQ=255)

                                             ggTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCgg                                                                                                                                                                                                      >  2:523262/1‑251 (MQ=255)

                                              gTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGt                                                                                                                                                                                                     <  1:643090/251‑1 (MQ=255)

                                                cgcgGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTAc                                                                                                                                                                                                   <  1:435343/251‑1 (MQ=255)

                                                   ggcatCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTg                                                                                                                                                                                                >  2:154638/5‑251 (MQ=255)

                                                    gcgTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGc                                                                                                                                                                                               <  1:154830/251‑1 (MQ=255)

                                                      gTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCGTGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCtt                                                                                                                                                                                             >  2:583940/1‑251 (MQ=255)

                                                               aTCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTaa                                                                                                                                                                                    <  1:571748/251‑1 (MQ=255)

                                                                      aGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGCGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACgcggcg                                                                                                                                                                             >  2:187406/1‑251 (MQ=255)

                                                                       gAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGgcgc                                                                                                                                                                            >  1:459343/1‑251 (MQ=255)

                                                                          aTCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGtt                                                                                                                                                                         <  1:226306/251‑1 (MQ=255)

                                                                          aTCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGtt                                                                                                                                                                         <  2:138708/251‑1 (MQ=255)

                                                                                 aTCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGc                                                                                                                                                                 <  1:57998/251‑1 (MQ=255)

                                                                                   cccGGATATCGATCAGGCTAAC‑TGCCTGATCAAACTAGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCgg                                                                                                                                                               >  2:303881/1‑251 (MQ=255)

                                                                                   cccGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCg                                                                                                                                                                <  1:392501/250‑1 (MQ=255)

                                                                                      ggATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGaaa                                                                                                                                                            <  2:372941/251‑1 (MQ=255)

                                                                                       gATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGaaaa                                                                                                                                                           <  1:421166/251‑1 (MQ=255)

                                                                                              aTCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACgg                                                                                                                                                    >  1:455527/1‑251 (MQ=255)

                                                                                                 aGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGgag                                                                                                                                                 <  1:522355/251‑1 (MQ=255)

                                                                                                   gCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGaa                                                                                                                                               <  1:240388/251‑1 (MQ=255)

                                                                                                    gctaacggatagATCAAACTGGAATCGGCCGGCCCCTTTATCGTTCTTTCGGGCGGGGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTCCTGCCCTGCCCCCCGCGGGACGGGCATGTTATCTTCGGTGTTCCCGATCATGTTTTGTTTGATAAAGGCGCTTATGCCTCGTCCGGTACTGGCTTCTCGGGTAACGCGGC‑CGTTTTGGCAGCGGAAATTCTTCGGGAGaa                                                                                                                                               <  2:657155/240‑1 (MQ=255)

                                                                                                     tAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGaaa                                                                                                                                              >  1:240497/1‑250 (MQ=255)

                                                                                                       aC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCTCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAAtt                                                                                                                                           <  1:11157/251‑1 (MQ=255)

                                                                                                              tGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTggtc                                                                                                                                     >  1:361663/1‑248 (MQ=255)

                                                                                                                 tCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCAc                                                                                                                                  >  1:88221/1‑251 (MQ=255)

                                                                                                                  cAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCAc                                                                                                                                  <  2:55372/250‑1 (MQ=255)

                                                                                                                         gAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTCCTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGgcgcgc                                                                                                                           >  2:218908/1‑250 (MQ=255)

                                                                                                                         gAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGgcgngc                                                                                                                           >  2:605189/1‑250 (MQ=255)

                                                                                                                           aTCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGCTATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGAACTTGCTTCTCGGGAAACGCGGCGCGTTT‑GGCCGCGGAACATCTACGGGCGAAAATTCTGTTCCACGGCGCGCaa                                                                                                                         >  2:530287/1‑250 (MQ=255)

                                                                                                                           aTCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCaa                                                                                                                         >  2:264426/1‑250 (MQ=255)

                                                                                                                             cGGACGGCACCTTTATCGGTCAGTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCCCCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAAt                                                                                                                       <  2:539052/250‑1 (MQ=255)

                                                                                                                                 cGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTgg                                                                                                                  <  1:434955/251‑1 (MQ=255)

                                                                                                                                  ggCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTggg                                                                                                                 <  1:593729/251‑1 (MQ=255)

                                                                                                                                     aCCTTTATCGTTCATTCTGGCGGTGCGGCTATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGCCGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTg                                                                                                               >  2:548413/1‑250 (MQ=255)

                                                                                                                                     aCCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCt                                                                                                                                         >  1:604352/1‑224 (MQ=255)

                                                                                                                                     aCCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCt                                                                                                                                         <  2:604352/224‑1 (MQ=255)

                                                                                                                                       cTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGGGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGATCCACGGCGCGCAAATGTTGGGTGAGc                                                                                                            >  1:379798/1‑251 (MQ=255)

                                                                                                                                         ttATTGTTCATTCTGGCGGTGCTGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGc                                                                                                            <  2:120910/249‑1 (MQ=255)

                                                                                                                                            tCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTg                                                                                                       >  1:484033/1‑251 (MQ=255)

                                                                                                                                             cGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAACCTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTGCGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTgg                                                                                                      <  1:155273/251‑1 (MQ=255)

                                                                                                                                              gTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGc                                                                                                     >  2:243352/1‑251 (MQ=255)

                                                                                                                                                     cTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCTTCGTCCGGTACTTGATTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAGAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGCGAGCCAGTGGCAGATGtt                                                                                              >  2:173706/1‑251 (MQ=255)

                                                                                                                                                       ggcggggcggATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCACATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATTTTGGGTGATCTAGTGGCAGATGTTCa                                                                                            <  1:477644/251‑1 (MQ=255)

                                                                                                                                                       ggCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTc                                                                                             >  1:612281/1‑250 (MQ=255)

                                                                                                                                                         cGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGAAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAAc                                                                                          >  1:596831/1‑251 (MQ=255)

                                                                                                                                                          ggTGCGGCTATTGGTACTGGTCTGGATACTGAAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACt                                                                                         >  1:565310/1‑251 (MQ=255)

                                                                                                                                                             gCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGc                                                                                                     <  1:341740/236‑1 (MQ=255)

                                                                                                                                                             gCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGc                                                                                                     >  2:341740/1‑236 (MQ=255)

                                                                                                                                                               ggATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCa                                                                                     >  1:639855/1‑250 (MQ=255)

                                                                                                                                                                         aCTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGcg                                                                           <  1:43054/250‑1 (MQ=255)

                                                                                                                                                                            ggTCTGGATACTGTAGTGACGAATCTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTAGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGcgtc                                                                         <  2:527878/249‑1 (MQ=255)

                                                                                                                                                                              tCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTgc                                                                     >  1:234283/1‑251 (MQ=255)

                                                                                                                                                                                 ggATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGcgcg                                                                  >  1:316686/1‑251 (MQ=255)

                                                                                                                                                                                     aCTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCa                                                               <  1:72893/250‑1 (MQ=255)

                                                                                                                                                                                        gTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAg                                                             <  1:583940/249‑1 (MQ=255)

                                                                                                                                                                                          aGTGACGAAACTGGCAGCAGAA‑GTGATGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGaa                                                           >  2:426340/1‑249 (MQ=255)

                                                                                                                                                                                           gTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAgg                                                        >  2:246983/1‑251 (MQ=255)

                                                                                                                                                                                           gTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAgg                                                        >  1:32174/1‑251 (MQ=255)

                                                                                                                                                                                             gACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGc                                                       <  1:83529/250‑1 (MQ=255)

                                                                                                                                                                                             gACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGc                                                       <  2:274447/250‑1 (MQ=255)

                                                                                                                                                                                              aCGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCg                                                      >  1:452453/1‑250 (MQ=255)

                                                                                                                                                                                                 aaaCTGGCAGC‑GAATATGCTGCACGGCCCGCCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGGGGAGGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAagt                                                  <  2:498996/251‑1 (MQ=255)

                                                                                                                                                                                                      ggCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAagttag                                               <  1:470999/249‑1 (MQ=255)

                                                                                                                                                                                                        cagcagAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTc                                           <  2:639855/251‑1 (MQ=255)

                                                                                                                                                                                                           cagAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTCCTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCggg                                        <  1:523262/251‑1 (MQ=255)

                                                                                                                                                                                                              aa‑GTGCTGCACTGCCCACCGCAGGCCGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGa                                      >  2:54419/1‑250 (MQ=255)

                                                                                                                                                                                                               a‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGata                                    <  2:480409/251‑1 (MQ=255)

                                                                                                                                                                                                               a‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGata                                    <  1:548413/251‑1 (MQ=255)

                                                                                                                                                                                                                   gctgcACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATAtt                                  <  2:316686/250‑1 (MQ=255)

                                                                                                                                                                                                                       cACTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGATTCGGGGATATTGccc                              >  1:487266/1‑250 (MQ=255)

                                                                                                                                                                                                                         cTGCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCAt                            >  2:468447/1‑250 (MQ=255)

                                                                                                                                                                                                                          ggCCCACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTTTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATaa                          <  2:243264/250‑1 (MQ=255)

                                                                                                                                                                                                                           gCCCACCGCAGGACGAGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCGAATGTTGGGGGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGGGCGCGGCAAGAAAGGCGAAGTTAGATTCGGGGATATTGCCCATaa                          >  2:343010/1‑250 (MQ=255)

                                                                                                                                                                                                                             ccACCGCAGGACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAgg                       >  2:655007/1‑251 (MQ=255)

                                                                                                                                                                                                                                    aGGAAGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAATAAAGGCGAAGTTAGTTTCGGGGATATTGCCCa                             <  1:558208/238‑1 (MQ=255)

                                                                                                                                                                                                                                    aGGAAGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCa                             >  2:558208/1‑238 (MQ=255)

                                                                                                                                                                                                                                      gACGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAaccgg              >  1:408567/1‑251 (MQ=255)

                                                                                                                                                                                                                                        cGTGCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAaccggc             >  1:31371/1‑250 (MQ=255)

                                                                                                                                                                                                                                           gCATGTTATCTTCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCGCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCTCATAAAGGCGAAaccggcaccg         <  2:279825/251‑1 (MQ=255)

                                                                                                                                                                                                                                                  aTCTTCGGTGCTACCGATCATGCGTTGTTTGATAACGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTgg  >  2:261641/1‑251 (MQ=255)

ATCCAATGGTCGTCACCGAAACCGCAAAATGGTGACTGGCACATCGGTCGCGGCGTCGCCATTATCATGCAGAAATCGGGTATCCCGGATATCGATCAGGCTAAC‑TGCATGATCAAACTGGAATCGGACGGCACCTTTATCGTTCATTCTGGCGGTGCGGATATTGGTACTGGTCTGGATACTGTAGTGACGAAACTGGCAGCAGAA‑GTGCTGCACTGCCCACCGCAGGACGTGCATGTTATCTCCGGTGATACCGATCATGCGTTGTTTGATAAAGGCGCATATGCCTCGTCCGGTACTTGCTTCTCGGGTAACGCGGCGCGTTT‑GGCAGCGGAAAATCTACGGGAGAAAATTCTGTTCCACGGCGCGCAAATGTTGGGTGAGCCAGTGGCAGATGTTCAACTGGCAACGCCGGGCGTCGTGCGCGGCAAGAAAGGCGAAGTTAGTTTCGGGGATATTGCCCATAAAGGCGAAACCGGCACCGGCTTTGG  >  NC_000913/3023209‑3023699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`