Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,255,644	G→A	A103A (GCC→GCT)	ycgV ←	putative autotransporter adhesin YcgV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,255,644	0	G	A	100.0%	108.9 / NA	33	A103A (GCC→GCT)	ycgV	putative autotransporter adhesin YcgV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/21);  total (12/21)

GGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCT‑GTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATCAGCAGCTATCAGCAGGGGGGAAAAGATAATTGCATTCAAACCAATAGCG  >  NC_000913/1255403‑1255860                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                          ggCCTATACCGTTCGAGTTTTCAATGGCGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTg                                                                                                                                                                                                                  <  1:541816/251‑1 (MQ=255)         ccGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGAc                                                                                                                                                                                                          <  1:630280/251‑1 (MQ=255)              cGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCCGGGAGCTGTTTTTGCCCTGAATAATGTTTCCTTTTGCATATCCAGAAGCGTCGTTGACATTTACGGttc                                                                                                                                                                                                     >  2:629934/1‑249 (MQ=255)                                    gTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCCTGGGTATCGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCCTATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGATGATAtt                                                                                                                                                                               >  2:394260/1‑251 (MQ=255)                                          gTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCa                                                                                                                                                                           <  2:483843/249‑1 (MQ=255)                                              gtgtTGAGCTATGCCCAATAATGTTGCCGTCATCGTTACTCTTAATGGTGCTTCCTTTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTTATGCTGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTCTTCATATCCAGAAGCGTCGTTTACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGcttg                                                                                                                                                                     <  2:527/251‑4 (MQ=255)                                                      cTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctgc                                                                                                                                                             >  1:50446/1‑251 (MQ=255)                                                           cccAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTgggg                                                                                                                                                        <  1:393761/251‑1 (MQ=255)                                                               ataatGATTCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCAt                                                                                                                                                    <  1:299442/251‑1 (MQ=255)                                                                taatGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCa                                                                                                                                                     >  1:431753/1‑249 (MQ=255)                                                                taatGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCa                                                                                                                                                     <  2:431753/249‑1 (MQ=255)                                                                    gatgCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTacac                                                                                                                                               >  2:532261/1‑251 (MQ=255)                                                                    gatgCCGTCATCGTTACGCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCCGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCGTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTACCTGCTGGGGTCATTaca                                                                                                                                                <  2:289571/250‑1 (MQ=255)                                                                     atgCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACAcc                                                                                                                                              >  1:516519/1‑251 (MQ=255)                                                                          gTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCAt                                                                                                                                            >  1:654435/1‑248 (MQ=255)                                                                           tCATCGTTACTCTTAATGGTGCTGCCTGTGCATAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGAAGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGATGTAAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTATTTCCTGCTGGGGTCATTACACCATag                                                                                                                                          >  1:609253/1‑249 (MQ=255)                                                                                         aaTGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGAt                                                                                                                          >  2:30462/1‑251 (MQ=255)                                                                                             gTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTTTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTTCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATcggc                                                                                                                      <  1:440880/251‑1 (MQ=255)                                                                                                  gcCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTAt                                                                                                                 <  1:135844/251‑1 (MQ=255)                                                                                                   ccTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTAt                                                                                                                 <  1:163983/250‑1 (MQ=255)                                                                                                      gtgCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTAtct                                                                                                               <  1:214302/249‑1 (MQ=255)                                                                                                        gCCTAAGTCAGCATGTGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACATCATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCT‑g                                                                                                          <  2:328901/251‑1 (MQ=255)                                                                                                        gCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCt                                                                                                            <  1:227029/250‑1 (MQ=255)                                                                                                         ccTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCT‑Gt                                                                                                         <  1:257193/251‑1 (MQ=255)                                                                                                         ccTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGAGTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCT‑Gt                                                                                                         <  2:557506/251‑1 (MQ=255)                                                                                                            aaGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCT‑GTaa                                                                                                       <  1:238241/250‑1 (MQ=255)                                                                                                                        gTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCT‑GTa                                                                                                        <  1:602200/237‑1 (MQ=255)                                                                                                                        gTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCT‑GTa                                                                                                        >  2:602200/1‑237 (MQ=255)                                                                                                                             gTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCT‑GTAATTCTGTCACCATCATTa                                                                                      >  1:631614/1‑250 (MQ=255)                                                                                                                                                         gagGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCT‑GTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTcc                                                         <  2:101713/251‑1 (MQ=255)                                                                                                                                                                              aCTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCT‑GTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATCAGCAGCTATcagcag                                    <  1:629934/251‑1 (MQ=255)                                                                                                                                                                                  tgAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGGATCTCCTTGTA‑TTCTGTCACCATTATTAATAGTAATATTGGTGCCCTATTTGCTTCCTGGATCAGCCGCttttcgccggggg                                >  2:240269/1‑239 (MQ=255)                                                                                                                                                                                                                 gCCCTGAATAATGATTCCTTTTGCATATCCAGAAGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCT‑GTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATCAGCAGCTATCAGCAGGGGGGAAAAGATAATTGCATTCAAACCAATAgcg  <  1:272921/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                          GGCCTATACCGTTCGAGTTTTCAATGGTGAATTGAGTGGCTGTTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCTGGGGTCATTACACCATAGAGGTTTCCTGATGGATCGGCGGTATCTCCT‑GTAATTCTGTCACCATCATTAATAGTAATATTGGTGCCATATTGACTTCCTGTATCAGCAGCTATCAGCAGGGGGGAAAAGATAATTGCATTCAAACCAATAGCG  >  NC_000913/1255403‑1255860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑