Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,117,082	G→A	I677I (ATC→ATT)	yghJ ←	putative lipoprotein YghJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,117,082	0	G	A	100.0%	229.7 / NA	68	I677I (ATC→ATT)	yghJ	putative lipoprotein YghJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/34);  total (34/34)

TACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGT  >  NC_000913/3116836‑3117326                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     tactacCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATc                                                                                                                                                                                                                                                   <  2:295161/250‑1 (MQ=255)  acnacCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCATCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCGCCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTGTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATca                                                                                                                                                                                                                                                  <  2:600199/250‑1 (MQ=255)        gACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCgg                                                                                                                                                                                                                                           <  2:545507/251‑1 (MQ=255)                cAGTGCCATCGACAGACCTTCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAATATTGCTCATCTCGTTTTCCATGATCAGCACCGCTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTTGTATCTCCACGCAGCGTGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCTGCATTTcc                                                                                                                                                                                                                                    <  1:196799/250‑1 (MQ=255)                     ccATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCgg                                                                                                                                                                                                                               >  1:528291/1‑250 (MQ=255)                       aTCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATc                                                                                                                                                                                                                            <  1:608329/251‑1 (MQ=255)                            cAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCg                                                                                                                                                                                                                           >  2:139348/1‑247 (MQ=255)                                   gCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCa                                                                                                                                                                                                                  <  1:619421/249‑1 (MQ=255)                                         tCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACt                                                                                                                                                                                                          >  2:354839/1‑251 (MQ=255)                                            aaCAGACGCACAAAACCAGACGCGCTCGCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGTATTTCCTGCGGATCGAGAGCGCCATAGCTACTTaa                                                                                                                                                                                                       <  2:30779/251‑1 (MQ=255)                                               aGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGGTCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTCCTTAAATg                                                                                                                                                                                                    >  1:278683/1‑251 (MQ=255)                                                     acaAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAAc                                                                                                                                                                                              <  2:528291/251‑1 (MQ=255)                                                             aGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGc                                                                                                                                                                                      <  2:658486/251‑1 (MQ=255)                                                             aGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGc                                                                                                                                                                                      >  1:428628/1‑251 (MQ=255)                                                              gACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGcc                                                                                                                                                                                     >  2:94018/1‑251 (MQ=255)                                                                 gcgcTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGc                                                                                                                                                                                  >  1:53959/1‑251 (MQ=255)                                                                       tctTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGACCGCCATAGCTACTTACATGCTCAACAGAGATGCCCGCAAAATc                                                                                                                                                                            >  1:633984/1‑251 (MQ=255)                                                                              tAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGCTCGCCATAGCCACTTAAATGCCCACCAGAGATGCCCGCAAAATCCGGATgg                                                                                                                                                                     >  2:554491/1‑251 (MQ=255)                                                                                   ttGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTc                                                                                                                                                                >  1:399950/1‑251 (MQ=255)                                                                                     gCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATAGGTCACATCCTGCTGAGTCAGCTTCGGGTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGGGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATAGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCg                                                                                                                                                               >  1:171068/1‑250 (MQ=255)                                                                                      cTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTNGa                                                                                                                                                              >  2:590249/1‑250 (MQ=255)                                                                                             cGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgcg                                                                                                                                                      <  1:401431/251‑1 (MQ=255)                                                                                              gTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCAtt                                                                                                                                                                                                                                       <  2:641853/169‑1 (MQ=255)                                                                                              gTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCAtt                                                                                                                                                                                                                                       >  1:641853/1‑169 (MQ=255)                                                                                              gTTTTCCATGATCAGCACCGATCCACCTATGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATCTTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAACTCCGGCTGGAAGTCGAACGCAgcgc                                                                                                                                                     >  2:316115/1‑251 (MQ=255)                                                                                                       gATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTg                                                                                                                                            >  1:505858/1‑251 (MQ=255)                                                                                                          cAGCACCGATCTACCTTTGTTCAGCTAGCCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGCTTGCATAAGGATAGTTACCCTCCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAACATCCGGATGGAATTCGCACGCCACGCTGTTTCCTGTa                                                                                                                                          >  2:572582/1‑250 (MQ=255)                                                                                                          cAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTgaa                                                                                                                                          >  2:492898/1‑248 (MQ=255)                                                                                                          cAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTa                                                                                                                                          >  2:27833/1‑250 (MQ=255)                                                                                                            gCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATcagcag                                                                                                                                                                                                                                              >  1:622017/1‑148 (MQ=255)                                                                                                            gCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATcagcag                                                                                                                                                                                                                                              <  2:622017/148‑1 (MQ=255)                                                                                                             cACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATTCTGCTGAGTCAGCTTCGGTCTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACGCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGCTCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGTTGTTTCCTGTAACCt                                                                                                                                      >  2:550795/1‑251 (MQ=255)                                                                                                               ccGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTgc                                                                                                                                    >  1:68856/1‑250 (MQ=255)                                                                                                                 gATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCt                                                                                                                                      <  1:561392/247‑1 (MQ=255)                                                                                                                 gATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCt                                                                                                                                      >  2:561392/1‑247 (MQ=255)                                                                                                                      cctcttGTTCAGATAAGCGATCAGATCGGTCACAACCTGCGGAGTCAGCTTCTGTTTGATGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCGCCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGCGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGCAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGAc                                                                                                                             <  2:232031/247‑1 (MQ=255)                                                                                                                               cAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAAt                                                                                                                    <  1:294959/251‑1 (MQ=255)                                                                                                                               cAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAAt                                                                                                                    <  2:311726/251‑1 (MQ=255)                                                                                                                                  aTAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGa                                                                                                                 <  1:46690/251‑1 (MQ=255)                                                                                                                                         aTCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATc                                                                                                          >  1:576445/1‑251 (MQ=255)                                                                                                                                           cAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCa                                                                                                        <  1:511617/251‑1 (MQ=255)                                                                                                                                            aGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATcc                                                                                                         >  1:285633/1‑249 (MQ=255)                                                                                                                                             gATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAgg                                                                                                      <  2:507003/251‑1 (MQ=255)                                                                                                                                              aacgtgcacatcctgcgtatgcAGCATCGGTGTGCTCGTATCTGATCACAGCTGGAATGCATAAGAATCGTTACCCACCTGAGTCCCATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTACCTGCGGATCGCGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACACAGCGCTGTTTCCTGTAAACTTACCATGTCGTTTAAAATTGACATTATCCAGGt                                                                                                     <  2:423408/230‑1 (MQ=255)                                                                                                                                              aTCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCa                                                                                                                                                                                 <  1:292027/175‑1 (MQ=255)                                                                                                                                              aTCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCa                                                                                                                                                                                 >  2:292027/1‑175 (MQ=255)                                                                                                                                                               gANTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCCCCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGTATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAAGCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGGTTAAAATAGACATTATCCAGGTTGGTGCCTACGGTCAt                                                                                     <  2:595365/250‑1 (MQ=255)                                                                                                                                                               gAGTCAGCTTCGGTTTGCGTGTATCTGCACGCAGCGGGATTGCATAATGATCGTTACCCACCTGAGTCACATTTTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTGCTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCTAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGCCAGTATCCAGGTTGGTGCCTACGGTc                                                                                       <  2:328583/248‑1 (MQ=255)                                                                                                                                                                 gTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCt                                                                                  <  2:551285/251‑1 (MQ=255)                                                                                                                                                                     gCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTc                                                                                       >  1:411039/1‑242 (MQ=255)                                                                                                                                                                     gCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTc                                                                                       <  2:411039/242‑1 (MQ=255)                                                                                                                                                                          ggTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCTGGCTTTcgc                                                                         <  1:421182/251‑1 (MQ=255)                                                                                                                                                                           gTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCTGGCTTTcgcg                                                                        >  1:13019/1‑251 (MQ=255)                                                                                                                                                                               gCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCTGGCTTTCGCGTCCg                                                                    >  1:498682/1‑251 (MQ=255)                                                                                                                                                                                  ggTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCTGGCTTTCGCGTCCGGc                                                                  <  1:590249/250‑1 (MQ=255)                                                                                                                                                                                  ggTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCTGGCTTTCGCGTCCGGCt                                                                 >  2:165816/1‑251 (MQ=255)                                                                                                                                                                                          cACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCTGGCTTTCGCGTCCGGCTTCCAttt                                                          >  1:272147/1‑250 (MQ=255)                                                                                                                                                                                                 cGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCgg                                                  <  1:500924/251‑1 (MQ=255)                                                                                                                                                                                                    gATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGAc                                                >  2:565389/1‑250 (MQ=255)                                                                                                                                                                                                            aaGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGc                                                                                   >  2:463580/1‑207 (MQ=255)                                                                                                                                                                                                            aaGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGc                                                                                   <  1:463580/207‑1 (MQ=255)                                                                                                                                                                                                              ggATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCgcag                                     <  1:190562/251‑1 (MQ=255)                                                                                                                                                                                                                        ccACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGtt                                                                                                                                                 >  2:369749/1‑133 (MQ=255)                                                                                                                                                                                                                        ccACCTGAGTCACATATTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGtt                                                                                                                                                 <  1:369749/133‑1 (MQ=255)                                                                                                                                                                                                                                       atTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATg            <  2:8814/251‑1 (MQ=255)                                                                                                                                                                                                                                       atTCAAAGCCGTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACGTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGGTTAAAATAGACAGTATCCAGGTTGTTGCCTACGGTCATGCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCTCGTTCTCCATGGAGTTCTTCAt             <  2:624418/250‑1 (MQ=255)                                                                                                                                                                                                                                                 ggtAAGAATCAGCAGCGGCATGTCCTGCGGAACGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTTTTTCCTGTAACCTGACCATGACGTTTAAAATAGGCAGTATCCGGGGTGGTGCCTACGGTCATGCGGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGTAGCACGTTGTCCATGAAGTTCTTCATGTAATCCGGGt  <  2:319875/249‑1 (MQ=255)                                                                                                                                                                                                                                                 gTTAAGAATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGt  >  1:110838/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     TACTACCGACTTGTTCAGTGCCATCGACAGACCTGCGGCATCCAACAGACGCACAAAACCAGACGCGCTCTCTTCCTTAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGTTTCCTGTAACCTGACCATGACGTTTAAAATAGACAGTATCCAGGTTGGTGCCTACGGTCATGCTGGCTTTCGCGTCCGGCTTCCATTTATCGTCGGACAGATAGCGCAGCACGTTCTCCATGAAGTTCTTCATGTCATCCGGGT  >  NC_000913/3116836‑3117326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑