Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,884,604	C→T	R38C (CGC→TGC)	rnpA →	RNase P protein component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,884,604	0	C	T	100.0%	202.2 / NA	59	R38C (CGC→TGC)	rnpA	RNase P protein component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/30);  total (29/30)

TGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTC  >  NC_000913/3884359‑3884841                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              tGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCCAATTACCATTCTCGGCTGCCTg                                                                                                                                                                                                                                          >  2:560600/1‑251 (MQ=255)  gTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGgctgcc                                                                                                                                                                                                                                            <  1:510561/248‑1 (MQ=255)       gAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTAACATTCTCGGCTGCCTGAAtt                                                                                                                                                                                                                                      <  1:440318/249‑1 (MQ=255)       gAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCATCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAANTCg                                                                                                                                                                                                                                    >  2:604016/1‑251 (MQ=255)        aaGCGCAACCGTTCTCACGGATTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCTCGCACGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCAACAACGGGCTGGCACGCCGCAAATTACCATTATCGGCTGCCTGATTTCGc                                                                                                                                                                                                                                   >  2:562438/1‑251 (MQ=255)            ccAACCGTTCTCACGCCTCCCGTGTTTGTATGGCTACTAAAAATGGCCGTCATGTTCTGTCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCGGAGTGGTCAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGGCAATCCACAGTCGTCTCCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATGCTCGGCTGCCTGAAGTCTCTggg                                                                                                                                                                                                                               <  2:435355/250‑1 (MQ=255)                  gTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATcc                                                                                                                                                                                                                         <  1:273738/251‑1 (MQ=255)                       cccGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCg                                                                                                                                                                                                                      <  2:246294/247‑1 (MQ=255)                          ggCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCg                                                                                                                                                                                                                 >  2:8470/1‑251 (MQ=255)                          ggCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCg                                                                                                                                                                                                                 >  2:443303/1‑251 (MQ=255)                           gCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCgg                                                                                                                                                                                                                <  2:645488/251‑1 (MQ=255)                                   gCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGGCCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACGCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACa                                                                                                                                                                                                         <  2:252400/250‑1 (MQ=255)                                      cGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTACCCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTc                                                                                                                                                                                                      <  1:562958/250‑1 (MQ=255)                                        tATGGCTACTAAAAATGGTCGTCAGGTTCTGGCCCGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGcc                                                                                                                                                                                                   >  1:211908/1‑251 (MQ=255)                                         aTGGCTACTAAAAATGGTCTTCAGGTTCTGGCTCGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCa                                                                                                                                                                                                  <  1:540937/251‑1 (MQ=255)                                                  aaaaaTGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGaaaaa                                                                                                                                                                                           <  1:400501/249‑1 (MQ=255)                                                    aaaTGGTCGTCAGGTTCTGGCACGTCTTCGTGCTAAAGGCCGCGCTCGTCTGACCTTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGtt                                                                                                                                                                                       <  1:106238/251‑1 (MQ=255)                                                               aGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCa                                                                                                                                                                            <  1:186328/251‑1 (MQ=255)                                                                 gTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATg                                                                                                                                                                          >  1:36611/1‑251 (MQ=255)                                                                                                       gACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATGCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCt                                                                                                                                      <  1:256645/249‑1 (MQ=255)                                                                                                       cggacgctcttccgatctaaGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACCCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTc                                                                                                                                    <  2:661210/233‑1 (MQ=255)                                                                                                          cGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGTGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGt                                                                                                                                 <  1:73364/251‑1 (MQ=255)                                                                                                           gTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGAGTGAAAGCTTCCGTc                                                                                                                                >  2:103937/1‑251 (MQ=255)                                                                                                             ttCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCt                                                                                                                               <  2:565475/250‑1 (MQ=255)                                                                                                                         aaGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTc                                                                                                                                    >  1:661210/1‑233 (MQ=255)                                                                                                                              aaCCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTATGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTcct                                                                                                             >  2:554523/1‑250 (MQ=255)                                                                                                                                 cccTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTTTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACAGGAACTCCTGGc                                                                                                          <  2:438982/251‑1 (MQ=255)                                                                                                                                  ccTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCCGTAAATTCACATTCGTCTTCCAGCAGCAACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAACCGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCt                                                                                                         >  1:117438/1‑251 (MQ=255)                                                                                                                                  ccTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATCCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCGTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGTATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCt                                                                                                         <  1:293898/251‑1 (MQ=255)                                                                                                                                   cTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCt                                                                                                         >  1:140629/1‑250 (MQ=255)                                                                                                                                   cTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCt                                                                                                         >  1:567806/1‑250 (MQ=255)                                                                                                                                     gAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATg                                                                                                      >  1:555042/1‑251 (MQ=255)                                                                                                                                      aGTGGTTAAGCTCGCCTTTCCCAGGGAGTTACGCTTGTTAACTACCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGc                                                                                                                                                                                                                             >  2:94223/1‑131 (MQ=255)                                                                                                                                      aGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACTGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGc                                                                                                                                                                                                                             <  1:94223/131‑1 (MQ=255)                                                                                                                                      aGTGGGTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTACCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGGCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGTTTAAACGTCTGCCGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCATGGCTATgg                                                                                                     >  2:441947/1‑251 (MQ=255)                                                                                                                                          gTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGAttt                                                                                                 <  1:297757/251‑1 (MQ=255)                                                                                                                                                   gCATTTCCCAGGGAGTTCCGCTTGTTATCTCTCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCgtggtggt                                                                                        <  2:472629/251‑1 (MQ=255)                                                                                                                                                          ccAGGGAGTTACGCTTGTTAACTCCCCGTCAATTCACATTCGTCTTCCAGCCGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGaa                                                                                  >  2:415340/1‑250 (MQ=255)                                                                                                                                                           cAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGaaaa                                                                                <  1:287178/251‑1 (MQ=255)                                                                                                                                                                    ccGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTg                                                                       <  2:211908/250‑1 (MQ=255)                                                                                                                                                                    aCGCTTGTTAACCCCCATCCAATTCACATTCGTCTTCCAGCGGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTg                                                                       <  2:94382/251‑1 (MQ=255)                                                                                                                                                                     cGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCAAAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGc                                                                      >  1:340634/1‑251 (MQ=255)                                                                                                                                                                              aCTCCCAGTCAATTCACATTCGTGTTCCAGCAGCCACAACGGGCTGGCACACCGCAAATTACCATTCTCGGCTGCCTGAATTCCCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGCACGCACTCGGATTAAACGTATGACGCGTGAAAGCTTCCGTCTGCGACAACATAAACTCCTGGCTATGGATTTCGTGGAGGTGGCGAAAAAAGGGGTTGCCGACCTCg                                                              >  2:67228/1‑250 (MQ=255)                                                                                                                                                                                ccccAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATa                                                           <  2:577717/250‑1 (MQ=255)                                                                                                                                                                                     gTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGACACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGATATGGATTTCGTGGTGGTGGCGGAAAAAGGGGTTGCCGACCTCGATAACCGt                                                      >  1:563830/1‑251 (MQ=255)                                                                                                                                                                                       cAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATg                                                                                                      <  2:552241/201‑1 (MQ=255)                                                                                                                                                                                       cAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACCCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATg                                                                                                      >  1:552241/1‑201 (MQ=255)                                                                                                                                                                                           tCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGctctc                                                >  1:463325/1‑251 (MQ=255)                                                                                                                                                                                               aTTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCgg                                            >  2:541461/1‑251 (MQ=255)                                                                                                                                                                                               aTTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCgg                                            >  2:241947/1‑251 (MQ=255)                                                                                                                                                                                                       tCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGt                                      <  2:612315/249‑1 (MQ=255)                                                                                                                                                                                                               ccACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAAtt                            >  1:167432/1‑251 (MQ=255)                                                                                                                                                                                                                       ggCTGGCACGCGGCAAATTACCATTCTCGGCTGCCTGACTTAGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAACCGCTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTATGCGCCAACATGAACTCCTGGCTATGGCTTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGcgcc                    >  2:255571/1‑251 (MQ=255)                                                                                                                                                                                                                           ggCACGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTGCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCa                <  2:167432/251‑1 (MQ=255)                                                                                                                                                                                                                              aCGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTg             >  1:465128/1‑251 (MQ=255)                                                                                                                                                                                                                              aCGCCGCAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTg             >  1:151455/1‑251 (MQ=255)                                                                                                                                                                                                                                 ccgcAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCg          <  1:73085/251‑1 (MQ=255)                                                                                                                                                                                                                                 ccgcAAATTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCg          <  2:299964/251‑1 (MQ=255)                                                                                                                                                                                                                                     aaaTTACCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTg      >  2:287604/1‑251 (MQ=255)                                                                                                                                                                                                                                          aCCATTCTCGGCTGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCTGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTc  <  1:8470/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              TGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTGCTCTCTCGGAAGCGTTGGAAAAATTATGGCGCCGCCACTGTCGCCTGGCTC  >  NC_000913/3884359‑3884841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑