Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,959,513	C→T	E112K (GAA→AAA)	yecM ←	putative metal‑binding enzyme YecM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,959,513	0	C	T	100.0%	198.8 / NA	61	E112K (GAA→AAA)	yecM	putative metal‑binding enzyme YecM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/31);  total (30/31)

TTACTCCTTAATTACGCCGACTGCTCGCTGGCAACGATCTCTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCC  >  NC_000913/1959269‑1959754                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  ttACTACTTAATTACCCCGACTGCTCGCTGGCAACGATCTCTTCAATGGACAACGGATGAAACTTAATAGTTGTTTTGCAATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAAATGGGTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGAGCACGGGCATTCAGCGTTTCTGGATCACCCGACAAAAAGACTTCAATCTGTTTCCAAcc                                                                                                                                                                                                                                             >  2:659217/1‑251 (MQ=255)     tCCTTAATTACGCCGACTGCTCGCTGGCAACGATCTCTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCTCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAAc                                                                                                                                                                                                                                              <  1:588810/246‑1 (MQ=255)     tCCTTAATTACGCCGACTGCTCGCTGGCAACGATCTCTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCg                                                                                                                                                                                                                                         >  2:514200/1‑251 (MQ=255)     tCCTTAATTACGCAGACTGCTCGCTGGCAACGATCTCTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAAc                                                                                                                                                                                                                                              >  2:588810/1‑246 (MQ=255)         tAATTACGCCGACTGCTCGCTGGCAACGATCTCTTAAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCgtg                                                                                                                                                                                                                                       <  2:54809/249‑1 (MQ=255)          aatggcGCCGACTGCTCGCTGACAACGATCTCTCCAATGGCCCACGGATCAAACTTAATAGGTGTTTTTCGATCTGTAACCACCGGCGTCGGATTCGGTAATCGCGCATGCTCACCTTTTGGTGAACTGGTTTTCACTGAGTTCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCTCACGGGCAGTCAGCGTTTCTGGATCACCCGGCACAACGATTGCAATATGTTTCCAACCGTCgtgagg                                                                                                                                                                                                                                    <  2:458704/246‑4 (MQ=255)              aCGCCGACTGCTCGCTGGCAACGATCTCTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTa                                                                                                                                                                                                                                 >  2:443988/1‑250 (MQ=255)                  cGACTGCTCGCTGGCAACGATCTCTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGtt                                                                                                                                                                                                                            >  1:494406/1‑251 (MQ=255)                   gACTGCTCGCTGGCAACGATCTCTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGttt                                                                                                                                                                                                                           >  1:156664/1‑251 (MQ=255)                      tGCTAGCTGGCAACGATCTCTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTTACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCACCCTTCGTGTGGGTAACGTTTTTc                                                                                                                                                                                                                        >  2:196600/1‑251 (MQ=255)                      cgCTCCCTGGCAACGACCACTTCAATTGACCACTGATGAAACTTAATAGTGGTTTTTCCATCGTGGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCATCCGGCAGAACGCTTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTc                                                                                                                                                                                                                        <  2:617584/250‑1 (MQ=255)                       gCTCGCTGGCAACGATCTCTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGttttt                                                                                                                                                                                                                         <  1:16618/249‑1 (MQ=255)                               gCAACGATCTCTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGcc                                                                                                                                                                                                                >  1:158091/1‑250 (MQ=255)                                cAACGATCTCTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACg                                                                                                                                                                                                                              >  2:535725/1‑235 (MQ=255)                                cAACGATCTCTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACg                                                                                                                                                                                                                              <  1:535725/235‑1 (MQ=255)                                      tctcTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGc                                                                                                                                                                                                        >  2:484430/1‑251 (MQ=255)                                          ttCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTcc                                                                                                                                                                                                     >  1:267975/1‑250 (MQ=255)                                                 gACCACGGATGCAACTTAATAGTTGTTTTGCCATCGGTGACCGCCCGCGTCAGATTCGGTAATCGCTAATGCTCACCTTTTGGAGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGCTTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCAGGGCCACGGTAGATCCACAAt                                                                                                                                                                                                >  2:260459/1‑248 (MQ=255)                                                  ccctcGGATGAACTTTATTAGTTGTTTTGACATGGGGGCCCGCCAGCGTCGGATTCGGTAATCACTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCTCGGTAGCTCCACAATAGaa                                                                                                                                                                                            <  2:471113/247‑1 (MQ=255)                                                           gAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGcc                                                                                                                                                                                     >  1:659646/1‑249 (MQ=255)                                                                 aaaTATTTGTTTTGCCATCGGTAGCCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCTGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCa                                                                                                                                                                             <  1:267297/250‑1 (MQ=255)                                                                          ttttGCCATCGGGGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGACAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCAAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATCGAAAACTGCCAGTGCGCAACCTGTAcc                                                                                                                                                                    >  2:5260/1‑251 (MQ=255)                                                                              gCCATCGGGGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCGCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGt                                                                                                                                                                 <  2:267975/250‑1 (MQ=255)                                                                                    ggTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGTTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCa                                                                                                                                                          <  1:64397/251‑1 (MQ=255)                                                                                      tGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAAt                                                                                                                                                        >  2:190157/1‑251 (MQ=255)                                                                                       gacagCCAGCGTCTGATTCGGTAATCGCACATGCTCACCTTATGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTTAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCGGTACCGGTTCATGTAAtt                                                                                                                                                       <  2:118305/247‑1 (MQ=255)                                                                                        aCCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAAttt                                                                                                                                                      >  1:331196/1‑251 (MQ=255)                                                                                         ccGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGCACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGACAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAAttt                                                                                                                                                      >  1:597869/1‑250 (MQ=255)                                                                                              aGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGCCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCACTGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAAc                                                                                                                                                  >  2:368315/1‑249 (MQ=255)                                                                                                          ggTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCt                                                                                                                                    <  1:190157/251‑1 (MQ=255)                                                                                                                    cATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATc                                                                                                                          <  1:55351/251‑1 (MQ=255)                                                                                                                     aTGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGTGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCCCGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCa                                                                                                                         <  1:442763/251‑1 (MQ=255)                                                                                                                        cTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCatat                                                                                                                      >  2:430584/1‑251 (MQ=255)                                                                                                                          cACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCatat                                                                                                                      <  1:260459/249‑1 (MQ=255)                                                                                                                           aCCTTTTGGCGAACTGGTTTTAACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAAACTGTACCGGTTCATGCACTTTAAACAGACAGATCGGTCTGCCATTGATCATAtttt                                                                                                                   >  2:532184/1‑251 (MQ=255)                                                                                                                                          ggTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAGATCGGTCTGCCATTGATCATATTTGCTGACAAAAGCTCg                                                                                                     <  1:532184/250‑1 (MQ=255)                                                                                                                                             tttCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCca                                                                                                  <  1:131530/250‑1 (MQ=255)                                                                                                                                                cACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACt                                                                                              <  1:554628/251‑1 (MQ=255)                                                                                                                                                      gaTCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCa                                                                                         >  1:510397/1‑250 (MQ=255)                                                                                                                                                       aTCCCCGGTAGTCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCTTTTCTTGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCGCAATAGAAATCTGCCAGTGCGCAACCTTTATCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCTTTGATCATATTTTCTGACGAAAGCTCGCCACGCTCTTCaaa                                                                                       <  2:275543/251‑1 (MQ=255)                                                                                                                                                                aGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGAc                                                                               <  1:628701/250‑1 (MQ=255)                                                                                                                                                                         ccTTCATCAGAAAGCAATGCCAGCGCACGGGCTTTCAGCGTTTCTGGATCACCCGGCAGAACGGTTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGGCACGGTAGCTGCACAATAGAAAACTGCCAGGGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAAGCGGTCTGCCATTGATCAGATTTTCTGACAAAAGCTCGCCACACTGTTCAAGCCCGCGACGCCGGCGTTc                                                                     <  2:422685/251‑1 (MQ=255)                                                                                                                                                                            tcatcaGAAAACAATGCCCGGGCAAGGGCATTCAGCGATTCTGGATCACCCGACAGAACGATTTCAATCTGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGACAACAGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAACCTCGCCACACTGTTCACAACCGCGACGCCAGCGTTcagc                                                                  >  2:617824/1‑251 (MQ=255)                                                                                                                                                                                caGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTg                                                              <  2:659646/251‑1 (MQ=255)                                                                                                                                                                                   aaaGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCg                                                           <  1:443988/251‑1 (MQ=255)                                                                                                                                                                                    aaGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGTCAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGt                                                          <  2:104860/251‑1 (MQ=255)                                                                                                                                                                                     aGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGtt                                                                                                                                                                <  1:99082/148‑1 (MQ=255)                                                                                                                                                                                     aGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGtt                                                                                                                                                                >  2:99082/1‑148 (MQ=255)                                                                                                                                                                                        aaTGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTg                                                      >  1:131267/1‑251 (MQ=255)                                                                                                                                                                                                       gCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCaa                                          >  2:214827/1‑248 (MQ=255)                                                                                                                                                                                                              gCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTTCCAACATTAGCGAGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTCAGCAACCTGTAACGGTCCATGCAATTTAAACAGACAAATCGGTCTGACATTGATCATATTTTCTGACAAAAGATCGCCACACTGTTCAAACCCGCGACGCAAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAATtgtg                                 >  1:620928/1‑250 (MQ=255)                                                                                                                                                                                                              gCGTTTCTGGATCACCCGGCAGAACGATTTCAATAGTTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCTCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGa                                <  1:659217/251‑1 (MQ=255)                                                                                                                                                                                                              gCGTTTCTGGATCACCCAGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAACCCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAAtgtg                                 >  2:276991/1‑250 (MQ=255)                                                                                                                                                                                                                      ggATCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGt                        <  2:9750/251‑1 (MQ=255)                                                                                                                                                                                                                       gATCACCCGGCGGAACGATTGCAATATCTTTCCAGCCTTCGTGTGGGTAACGTGTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAAGTTAAACAGACAAATCGGTCTGCCATTGATCATAGTGTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTc                       <  2:235160/251‑1 (MQ=255)                                                                                                                                                                                                                        aTCACCCGGCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTc                       >  1:54695/1‑250 (MQ=255)                                                                                                                                                                                                                               ggCAGAACGATTTCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGt               <  1:196600/251‑1 (MQ=255)                                                                                                                                                                                                                               ggCAGAACGATTTCAATATGTTTCCAACATTCGTGTGGGAAACGTTTTTCCCCGGACCACGGTAGCTCCACAATAGAAAACTGCCAGTACGCAACCTGTACAGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATCGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATATGATCGTCAGTCAGCGGAgg               >  2:558500/1‑250 (MQ=255)                                                                                                                                                                                                                                          ttCAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAAc     <  1:439657/250‑1 (MQ=255)                                                                                                                                                                                                                                            cAATATGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAAccc   <  1:608007/250‑1 (MQ=255)                                                                                                                                                                                                                                              atatGTTTCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAAcccc  >  2:108879/1‑249 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  TTACTCCTTAATTACGCCGACTGCTCGCTGGCAACGATCTCTTCAATGGACCACGGATGAAACTTAATAGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCC  >  NC_000913/1959269‑1959754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑