Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	910,080	C→T	E324K (GAA→AAA)	poxB ←	pyruvate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	910,080	0	C	T	100.0%	202.3 / NA	60	E324K (GAA→AAA)	poxB	pyruvate oxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/26);  total (34/26)

TAAACGAACCTAACAGGCGACGCTTGCCGTTCATTTTTAGATAACGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTCCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGT  >  NC_000913/909840‑910325                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      tAAACGAACCTAACAGGCGACGCTTGCCGTTCATTTTTAGATAACGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGGGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGTTTTTTCTTTCACCAATgg                                                                                                                                                                                                                                              >  2:274914/1‑250 (MQ=255) tAAACGAACCTAACAGGCGACGCTTGCCGTTCATTTTTAGATAACGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGAGGGCGTCGCGGTAATCTTCCAGCGGTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATgg                                                                                                                                                                                                                                              >  1:513182/1‑250 (MQ=255)  aaaCGAACCTAACAGGCGACGCTTGCCGTTCATTTTTAGATAACGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGa                                                                                                                                                                                                                                             >  2:165059/1‑250 (MQ=255)    aCGAACCTAACAGGCGACGCTTGCCGTTCATTTTTAGATAACGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGc                                                                                                                                                                                                                                          >  1:231095/1‑251 (MQ=255)       aaCCTAACAGGCGACGCTTGCCGTTCATTTTTAGATAACGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTTCGGTCGGCTTTTTCTTTCACCAATGGAAGCAAt                                                                                                                                                                                                                                       >  2:323657/1‑251 (MQ=255)       aaCCTAACAGGCGACGCTTGCCGTTCATTTTTAGATAACGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTTTTTCACCAATGGAAGCAAt                                                                                                                                                                                                                                       >  2:58563/1‑251 (MQ=255)                gtcNACGCTTGCCGTTCATTGTTAGATAACGTGCCGCCCACACCGTTGGCGGACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAAGGACTAATTTGCTTCGTCAGATATTGCGGGTGATTGTCTTTCTCGCTCGGTTTAGCTAAGTCGTCCAGCCCTTTGCTGGCGTCGCGGTAATCTTCCAGCGGTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCTCCAATGGGATCAATGCGCGCaga                                                                                                                                                                                                                              <  2:603034/249‑1 (MQ=255)                  cGACGCTTGCCGTTCATTTTTAGATAACGTGCCGGCCGCACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGTCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTTCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAGCTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCTGAGt                                                                                                                                                                                                                            <  2:466226/251‑1 (MQ=255)                             gTTCATTTTTAGATAACGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCAGCGGCAAAATGACTAATTTGCTTCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGGAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGatat                                                                                                                                                                                                                 >  1:4027/1‑251 (MQ=255)                                cATTTTTAGATAACGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGc                                                                                                                                                                                                              <  2:468376/251‑1 (MQ=255)                                   ttttAGATAACGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCg                                                                                                                                                                                                            >  2:413666/1‑250 (MQ=255)                                     ttAGATAACGTGCCGCCCACACCGTTGGCGTACCAAAGTCACAGGTGAAAATAGCGTCATCGGCGGAAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGGGCTTTATCCAGAAACTTGAGCTCGGCTTTTTTTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGAcc                                                                                                                                                                                                         >  1:492944/1‑251 (MQ=255)                                         aTAACGAGCCGCGCACACCGTTGGCGTACCAACGTCAAAGGTGAAAATAACGTCATCGGCGGCAAAATGACTAATTTGCTGGGCCAGATCTTGCGGGTGAAAGGCATTCTCGCTCGGTTTAGCTAAATCGTCCAGCACTTTCCGGGCGTCGCGGTAATCTTCAAGCGCTTTATCAAGGAACTTGCGATTGTCTTTTTCTTTAAACAATAGAATGAATGCACACAGAGTCGACGTGATTTCGCAGACCatcg                                                                                                                                                                                                     >  2:296106/1‑248 (MQ=255)                                          tAACGTGCCGCCCACACCGTTGGCGGACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGAATAATTTGCTGCGCCAGATATTGCGGGGGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAgggc                                                                                                                                                                                                    >  1:519028/1‑248 (MQ=255)                                           aaCGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGcc                                                                                                                                                                                                   >  1:33104/1‑251 (MQ=255)                                            aCGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGcc                                                                                                                                                                                                   >  1:389558/1‑250 (MQ=255)                                             cGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCCGCAAAATGACTCATTTGCTGCGGCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGACACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACTCAGAGTCGACTTGATATCGCCTCCCAGTTCCat                                                                                                                                                                                                 >  2:215821/1‑251 (MQ=255)                                                 ccgccCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATcc                                                                                                                                                                                             <  1:552373/251‑1 (MQ=255)                                                      cacaCCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCtt                                                                                                                                                                                        <  1:519562/251‑1 (MQ=255)                                                       acacCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTg                                                                                                                                                                                       <  1:129856/251‑1 (MQ=255)                                                                  gTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGcc                                                                                                                                                                            <  2:491647/251‑1 (MQ=255)                                                                     ccAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGAt                                                                                                                                                                         <  2:533932/251‑1 (MQ=255)                                                                                         aGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGCATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGAc                                                                                                                                                                                                                        >  1:395240/1‑184 (MQ=255)                                                                                         aGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCTCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGAc                                                                                                                                                                                                                        <  2:395240/184‑1 (MQ=255)                                                                                               aTCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCTGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGaa                                                                                                                                               <  1:96687/251‑1 (MQ=255)                                                                                               aTCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGGGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGGAATCTTCCCGCGCTTTATCCAGAAACTTTCGATCGGCTTTTTCTTTCACCAATGGAAACAATGCACGCAGAGTCGACTTGATATCGCCGACCAGGGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGGTATCAATCTGa                                                                                                                                                >  1:272051/1‑250 (MQ=255)                                                                                                 cggcggCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATg                                                                                                                                             >  1:57334/1‑251 (MQ=255)                                                                                                             gACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGAATTTGGCAtcg                                                                                                                                 >  1:522828/1‑251 (MQ=255)                                                                                                                 aaTTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCAtcggt                                                                                                                               <  1:616436/249‑1 (MQ=255)                                                                                                                         gcgcCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGa                                                                                                                       <  2:283631/249‑1 (MQ=255)                                                                                                                                atatTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGt                                                                                                              >  2:544413/1‑251 (MQ=255)                                                                                                                                  atTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGt                                                                                                              >  2:104658/1‑249 (MQ=255)                                                                                                                                  atTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTa                                                                                                             <  2:58898/250‑1 (MQ=255)                                                                                                                                     gCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGa                                                                                                         <  1:426473/251‑1 (MQ=255)                                                                                                                                           gAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGc                                                                                                   <  1:58563/251‑1 (MQ=255)                                                                                                                                             aTGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGAAGAAGGCGCGGTAGGGAAATTGCGt                                                                                                 >  2:363654/1‑251 (MQ=255)                                                                                                                                                  tttCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTAGCCAGAAACTTGCGTTCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTTGCATCGGTCGGGTAGAAGGCGCGGTCGGGAAATTGCGAGCCg                                                                                             >  1:124713/1‑250 (MQ=255)                                                                                                                                                    tctcGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGTAAATTGCGTGCCGAg                                                                                           <  1:506586/250‑1 (MQ=255)                                                                                                                                                       cgctcgGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGt                                                                                          <  1:138968/248‑1 (MQ=255)                                                                                                                                                       cgctcgGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAg                                                                                        <  1:363654/250‑1 (MQ=255)                                                                                                                                                       cgctcgGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGc                                                                                       >  1:289355/1‑251 (MQ=255)                                                                                                                                                         ctcgGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCCCCAATGGCAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGCGAGCGCCGACGCTGGCTGGGATGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCCCGGtccgtacatggcgtcgcgagtagcac                                                                                     >  2:528671/1‑226 (MQ=255)                                                                                                                                                         ctcgGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGACCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGATGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCAc                                                                                     >  1:397389/1‑251 (MQ=255)                                                                                                                                                            ggTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATCTCAATCTGAATGAGTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTaa                                                                                  <  1:428817/251‑1 (MQ=255)                                                                                                                                                              tttAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGGTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACg                                                                                >  1:202027/1‑251 (MQ=255)                                                                                                                                                                 aGCTAAATCGTCCAGCCCTTTGCGGGCGTTGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACGTGATATCGCCGACCAGTGCCATATCCCCCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACgtgt                                                                             <  1:379257/251‑1 (MQ=255)                                                                                                                                                                         cGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTtc                                                                     >  1:494221/1‑251 (MQ=255)                                                                                                                                                                          gTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTATAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTATCGTGTCGGCGTtca                                                                    <  2:624998/251‑1 (MQ=255)                                                                                                                                                                           tCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCCTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGAGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTtcat                                                                   >  2:443159/1‑251 (MQ=255)                                                                                                                                                                                        gggCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGaa                                                       >  1:390648/1‑250 (MQ=255)                                                                                                                                                                                           cGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGTAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTTAGCTCCGATGCTGGCGGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTTGAAGGCGCGGTAGGGAAATGGCGTGCCGTGTAGCTCTAACGTGTCGGCTTGCATCATGGTATGGAAACCt                                                   <  2:641130/251‑1 (MQ=255)                                                                                                                                                                                               gcgGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCCCGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTTAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGTCGTTCATCATGGTATGGAACCCTGACg                                               <  2:274684/251‑1 (MQ=255)                                                                                                                                                                                                  gTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGACGACTTGATATCGCCGACCAGTGCCATATCCAACTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATAGGTCGGGTAGAAGGCGCGGAAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGACACCTGACg                                               >  1:385916/1‑248 (MQ=255)                                                                                                                                                                                                         tCCAGCGCTTTATCCAGAAACTTGCGTTCGGCTTTTTCTTTCACCAATGGAATCAATGCGCGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGTTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTATGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGc                                         <  2:397389/247‑1 (MQ=255)                                                                                                                                                                                                          ccAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGAtt                                    >  1:409616/1‑251 (MQ=255)                                                                                                                                                                                                                    aTNCAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAAGTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTc                           <  2:586927/250‑1 (MQ=255)                                                                                                                                                                                                                      ccAGAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCTTCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCAtt                        >  1:609965/1‑251 (MQ=255)                                                                                                                                                                                                                         gAAACTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGGAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCAGGTCATTcc                      >  2:475577/1‑250 (MQ=255)                                                                                                                                                                                                                           aaCTTGCGATCGGCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCTGGCATTCCAAc                   <  2:272051/251‑1 (MQ=255)                                                                                                                                                                                                                                      ggCTTTTTCTTTCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGAt        >  1:482650/1‑251 (MQ=255)                                                                                                                                                                                                                                            ttctttCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGt  >  2:454742/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      TAAACGAACCTAACAGGCGACGCTTGCCGTTCATTTTTAGATAACGTGCCGCCCACACCGTTGGCGTACCAACGTCACAGGTGAAAATAGCGTCATCGGCGGCAAAATGACTAATTTGCTGCGCCAGATATTGCGGGTGAATGGCTTTCTCGCTCGGTTTAGCTAAATCGTCCAGCCCTTTGCGGGCGTCGCGGTAATCTTCCAGCGCTTTATCCAGAAACTTGCGATCGGCTTTTTCTTCCACCAATGGAAGCAATGCACGCAGAGTCGACTTGATATCGCCGACCAGTGCCATATCCACCTTGCTGTGAGCGCCGATGCTGGCTGGGTTGATATCAATCTGAATGATTTTGGCATCGGTCGGGTAGAAGGCGCGGTAGGGAAATTGCGTGCCGAGTAGCACTAACGTGTCGGCGTTCATCATGGTATGGAAACCTGACGAGAAGCCGATTAACCCGGTCATTCCAACATCATACGGATTATCGT  >  NC_000913/909840‑910325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑