Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	269,161	C→T	intergenic (‑201/+128)	ykfA ← / ← perR	putative GTP‑binding protein YkfA/putative transcriptional regulator PerR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	269,161	0	C	T	100.0%	140.1 / NA	41	intergenic (‑201/+128)	ykfA/perR	putative GTP‑binding protein YkfA/putative transcriptional regulator PerR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/21);  total (20/21)

CAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGCCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGATCAACGAATTTTACCCAGATTGAGTTCATTCAGTAGCCAGGTTTTGAACACATCAAGCGCAGAATGCATGTGTTGGTGCTGGGGGAAAACCAAATAATGACCGATGT  >  NC_000913/268915‑269395                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           cAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGATATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGATCcgtcgt                                                                                                                                                                                                                                         <  2:637204/250‑1 (MQ=255)  aGCAAGCGATTGCTGAAACGACTTAAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGTAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGa                                                                                                                                                                                                                                       <  2:486563/251‑1 (MQ=255)        cGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACAcc                                                                                                                                                                                                                                 <  1:593884/251‑1 (MQ=255)                   aCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCa                                                                                                                                                                                                                      <  1:586565/251‑1 (MQ=255)                          cAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGc                                                                                                                                                                                                               <  1:123658/251‑1 (MQ=255)                               cTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGt                                                                                                                                                                                                          >  2:120143/1‑251 (MQ=255)                                ttCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGtt                                                                                                                                                                                                         >  1:461006/1‑251 (MQ=255)                                 tCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGttt                                                                                                                                                                                                        >  1:121995/1‑251 (MQ=255)                                       ttattCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGc                                                                                                                                                                                                  >  1:582814/1‑251 (MQ=255)                                         attCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCcg                                                                                                                                                                                                <  1:338567/251‑1 (MQ=255)                                                           gAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGagag                                                                                                                                                                                <  1:512025/249‑1 (MQ=255)                                                                ataataAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTcac                                                                                                                                                                         <  2:203204/251‑1 (MQ=255)                                                                ataataAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTcac                                                                                                                                                                         >  1:501215/1‑251 (MQ=255)                                                                  aataaAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACaa                                                                                                                                                                       <  2:336650/251‑1 (MQ=255)                                                                          aCGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGc                                                                                                                                                               <  1:74427/251‑1 (MQ=255)                                                                          aCGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAAGCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTca                                                                                                                                                                          >  1:273522/1‑240 (MQ=255)                                                                          aCGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAAGCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTca                                                                                                                                                                          <  2:273522/240‑1 (MQ=255)                                                                                     gTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGCTTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGCGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGtaatac                                                                                                                                                     >  2:293506/1‑249 (MQ=255)                                                                                       gagaTTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAg                                                                                                                                                   <  1:332180/250‑1 (MQ=255)                                                                                             ccgccgTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACtt                                                                                                                                             <  2:135154/250‑1 (MQ=255)                                                                                                     aaTTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACCCCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGATCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCag                                                                                                                                    <  2:383300/251‑1 (MQ=255)                                                                                                     aaTTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCag                                                                                                                                    <  1:485166/251‑1 (MQ=255)                                                                                                                ttCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGAtt                                                                                                                          >  2:620982/1‑250 (MQ=255)                                                                                                                             tatatatCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGAt                                                                                                            >  1:558343/1‑251 (MQ=255)                                                                                                                             tatatatCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGAt                                                                                                            >  1:287187/1‑251 (MQ=255)                                                                                                                              atatatCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGAt                                                                                                            >  1:73042/1‑250 (MQ=255)                                                                                                                                 tatCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGAt                                                                                                            >  2:4052/1‑247 (MQ=255)                                                                                                                                 tatCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGAt                                                                                                            <  1:4052/247‑1 (MQ=255)                                                                                                                                                     ccTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGATCAACGAATTTTACCCAGATTGAGt                                                                                    <  1:636404/251‑1 (MQ=255)                                                                                                                                                                         gcgcGACTGCTGGCTTAGGCAATGAATGAGTTATAATAGCAGAATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGATCAACGAATTTTACCCAGATTGAGTTCATTCAGTAGCCAGGttt                                                                 <  2:107307/250‑1 (MQ=255)                                                                                                                                                                             gtctgctgGCTTAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACATGGATTTATTGAGAGTATGATCCTTCGTGATACACCTTAAAATCGCCACCCATCAATGTATCGGAGCCGCGAGCATAAGTGAGAACCTTCACAAAATAATGCTTATGAATAAAGTTACTTTGAATTCATAGCCCCTATTAATGTGACCATGATCATCGAATTTTACCCAGAATGACTTCATTCAGTAGCCAGGTTTTGAac                                                            <  2:454308/249‑1 (MQ=255)                                                                                                                                                                                 gctgGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGATCAACGAATTTTACCCAGATTGAGTTCATTCAGTAGCCAGGTTTTGAACACAt                                                        >  1:238920/1‑251 (MQ=255)                                                                                                                                                                                 gctgGCTCAGGCAATGAAGGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGACGTCGTGATACACATTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGAGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGATCAACGAATTTTACCCAGATTGAGTTCATTCAGTAGCCAGGTTTTGAACACAt                                                        <  1:253992/251‑1 (MQ=255)                                                                                                                                                                                         aGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGATCAACGAATTTTACCCAGATTGAGTTCATTCAGTAGCCAGGTTTTGAACACATCAAgcgc                                                 >  2:414460/1‑250 (MQ=255)                                                                                                                                                                                         aGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGATCAACGAATTTTACCCAGATTGAGTTCATTCAGTAGCCAGGTTTTGAACACATCAAgcgc                                                 >  2:566880/1‑250 (MQ=255)                                                                                                                                                                                          ggCAATGAATGAGTGATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGACGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGATTAACGAATTTTACCCAGAGTGAGTTCATTCAGTAGCCAGGTTTTGAACACATCAAGCGCAg                                               <  2:297242/251‑1 (MQ=255)                                                                                                                                                                                                    gaGTTATAATAGTAGCATTTACTAACAGGACTTTATAGAGCGTATGAGCCGTCGTGATACACCTTAAAATATCAAACCAGCAAAGTGGCGGAGTCGCGAGAAAAAGAGATAGCCATCACAACATAATGCTTAGTAATACAGTTACTTTGAATTCAGAGCCCAGATTAATGTGCATATGATCAACGAATTTTACACAGATTGAGTTCATTAAGTAGCCAGGTTTTGAACACATCAAGCGCAGAATGCAggtg                                     >  2:189810/1‑251 (MQ=255)                                                                                                                                                                                                      gTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGATCAACGAATTTTACCCAGATTGAGTTCATTCAGTAGCCAGGTTTTGAACACATCAAGCGCAGAATGCATGTGtt                                   >  1:61527/1‑251 (MQ=255)                                                                                                                                                                                                               gcagcaTTTAATAACAGGGAGTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTAACTTTGAATTCAGAGCCCCGATTAATGTGAACATGATCAACGAATTTTACCCAGATTGAGTTCATTCAGTAGCCAGGTTTTGAACACATCAAGCGCAGAATGCATGTGTTGGTGCTggg                          >  2:201407/1‑251 (MQ=255)                                                                                                                                                                                                                   caTTTACTAACAGGGATTTATTGAGAGTATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGAGTAATGTGAACATGATCAACGAATTTTACCCAGATTGAGTTCATTCAGTAGCCATGTTTTGAACACATCAAGCGCAGAATGCATGTGTTGGTGCTGGGGGaa                      <  1:254290/251‑1 (MQ=255)                                                                                                                                                                                                                           aaCAGGGATTTATTGAGAGAATGAGCCGTCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGATAAACGAATTTTACCCAGATTGAGTTCATTCAGTAGCCAGGTTTTGAACACATCAAGCGCAGAATGCATGTGTTGGTGCTGGGGGAAAACCAaat              >  2:198805/1‑251 (MQ=255)                                                                                                                                                                                                                                        ttagagTATGAGGCGTCGTGATACACATTAAAATCTCAACCCAGCACAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCACCGATTAATGTGAACATGATCAACGAATTTTACCCAGATTGAGATCATTCAGTAGCCAGGTTTTGAACACATCAAGCGCAGACTGCATGTGTTGGTGCTGGGGGAAAACCAAATAATGACCGatgt  >  2:385383/3‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           CAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTTCAATCCTCTGCGCTTGAGGCAGCTGCGCGACTGCTGGCTCAGGCAATGAATGAGTTATAATAGCAGCATTTACTAACAGGGATTTATTGAGAGTATGAGCCGCCGTGATACACCTTAAAATCTCAACCCAGCAAAGTTTCGGAGCCGCGAGCAAAAGTGAGAGCCTTCACAAAATAATGCTTAGTAATAAAGTTACTTTGAATTCAGAGCCCCGATTAATGTGAACATGATCAACGAATTTTACCCAGATTGAGTTCATTCAGTAGCCAGGTTTTGAACACATCAAGCGCAGAATGCATGTGTTGGTGCTGGGGGAAAACCAAATAATGACCGATGT  >  NC_000913/268915‑269395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑