Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,290,881	C→T	L214F (CTT→TTT)	rssB →	regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,290,881	0	C	T	100.0%	88.6 / NA	27	L214F (CTT→TTT)	rssB	regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/10);  total (17/10)

GGAAGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGC  >  NC_000913/1290643‑1291121                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 ggaagaggaaAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGt                                                                                                                                                                                                                                      >  2:207060/1‑251 (MQ=255)                                                           gcgAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACg                                                                                                                                                                            >  1:432557/1‑251 (MQ=255)                                                           gagaaattttgaatgaaccTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACg                                                                                                                                                                            <  1:353482/233‑1 (MQ=255)                                                              aaaTTATTAGAGCACCTACAACCGTCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGtt                                                                                                                                                                         <  1:418509/251‑1 (MQ=255)                                                               aaTTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTg                                                                                                                                                                        >  2:577523/1‑251 (MQ=255)                                                                    ttaCAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGcc                                                                                                                                                                      >  2:641132/1‑248 (MQ=255)                                                                                  aCCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACCGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTAt                                                                                                                                                     >  1:238603/1‑251 (MQ=255)                                                                                   ccgccgGTTCAGCTGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCAGTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTAt                                                                                                                                                     <  2:548852/250‑1 (MQ=255)                                                                                                aggagaattCCCATTGTCGGGTTCATTCTCGTCAATTGGTAGAGGCGGACCAACCCGGCCTGGTGCTTCATATTGCCGCACTTTCGGGAAACGATCTAGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAAc                                                                                                                                       >  2:59508/8‑251 (MQ=255)                                                                                                          ccATTGCCGTGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTTATATTGCCGCACTGTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCTCACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTc                                                                                                                             <  2:119575/251‑1 (MQ=255)                                                                                                               gCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTc                                                                                                                          >  2:503352/1‑249 (MQ=255)                                                                                                                    gTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGAGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCCTTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTACCGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGATTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCa                                                                                                                    >  2:394853/1‑250 (MQ=255)                                                                                                                                  aTTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGt                                                                                                     <  2:444360/251‑1 (MQ=255)                                                                                                                                            gcggaaaacaccGGCCTGGTGCTTGATATTTCCGCACTTTCGGAAAACGATTAGGCATTTTATTGCCTTGATGGTC‑CCCGCGCTGGGCATAATGGCGTATTTGCTGCCTTGTTATTCCGCGCATTGTTTAACGGGTTATTTCAGGAACAGCTTGCACACCAAAAACAACGGTTGCCAGAGTTGTGCGCGTTATTGAAGCAGGTAAACCATTTACGTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTAtt                                                                                           <  1:188993/241‑1 (MQ=255)                                                                                                                                                           cTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGcgcg                                                                                                                                                         <  2:540579/174‑1 (MQ=255)                                                                                                                                                           cTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGcgcg                                                                                                                                                         >  1:540579/1‑174 (MQ=255)                                                                                                                                                              gTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATATTGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGTTTGCCATAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATcg                                                                         <  1:391012/251‑1 (MQ=255)                                                                                                                                                                       atTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGa                                                                 >  1:430097/1‑250 (MQ=255)                                                                                                                                                                              cACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAAtctc                                                         >  1:353546/1‑251 (MQ=255)                                                                                                                                                                                         aaCGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATACTGGCGTATTTGCTGCCTTGTTCTTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGACCAATCTGCCGGGGGAGTTTCCGCTATTAGTTGGCTATTATCATCGCGGACTGAAACATCTCATTCTGGTTTc                                              >  2:63794/1‑251 (MQ=255)                                                                                                                                                                                                             ccTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCATTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATAGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGCCGt                          >  1:379411/1‑251 (MQ=255)                                                                                                                                                                                                              cTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATcgc                                                                        <  2:581692/204‑1 (MQ=255)                                                                                                                                                                                                              cTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATcgc                                                                        >  1:581692/1‑204 (MQ=255)                                                                                                                                                                                                              cTTGATG‑TCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGGTTCTGCGGGTCTGAATGCGACGtt                         >  1:355636/1‑251 (MQ=255)                                                                                                                                                                                                                             aGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTaaa                                                                                                                                        >  2:192816/1‑126 (MQ=255)                                                                                                                                                                                                                             aGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTaaa                                                                                                                                        <  1:192816/126‑1 (MQ=255)                                                                                                                                                                                                                                      tAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGc  >  1:540024/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 GGAAGAGGAAAGGCTTTTTCGCGACTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATG‑TCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGC  >  NC_000913/1290643‑1291121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑