Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,338,426 G→A I12I (ATC→ATT)  mlaD ← intermembrane phospholipid transport system, substrate binding protein MlaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,338,4260GA98.2% 166.6 / NA 54I12I (ATC→ATT) mlaDintermembrane phospholipid transport system, substrate binding protein MlaD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (27/26);  minor base C (1/0);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGATCCAT  >  NC_000913/3338181‑3338660
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          
ggAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATa                                                                                                                                                                                                                                         <  1:543828/249‑1 (MQ=255)
   aTGTGGTTATACCGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCGTTCAGGCCGCCAATGTTATTGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATtcccac                                                                                                                                                                                                                                    <  1:657962/251‑1 (MQ=255)
   aTGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACGGTCCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTGCGCCGCCTTCAGGCAAGCAAACAGCGCCGCCGGCAGTGCGGCTAATATAAAAATacccac                                                                                                                                                                                                                                    <  2:37989/251‑1 (MQ=255)
                   gttCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCCCCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCAtttttt                                                                                                                                                                                                                    <  2:396009/251‑1 (MQ=255)
                        aTTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGt                                                                                                                                                                                                               >  2:85188/1‑251 (MQ=255)
                        aTTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGGCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGt                                                                                                                                                                                                               >  2:523879/1‑251 (MQ=255)
                         tttCCAGCGTTAAGCGCGGCAGATAGGTTTTCGGGTCCAGCGTACTATCCGCCACCAGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCCCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCACCGCCACCAGTGCTGCTACTACACAAATACCCACCCAAATTTCATTTTTTTTCGtt                                                                                                                                                                                                              >  2:547912/1‑251 (MQ=255)
                           tCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTg                                                                                                                                                                                                            >  2:369133/1‑251 (MQ=255)
                            ccAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGc                                                                                                                                                                                                           <  1:369133/251‑1 (MQ=255)
                                 gTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCCCCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAAt                                                                                                                                                                                                                              <  2:610796/227‑1 (MQ=255)
                                 gTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAAt                                                                                                                                                                                                                              >  1:610796/1‑227 (MQ=255)
                                  ttACGCGCGGCAGATAGGTTTTCGGGTCCATCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGGATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGaa                                                                                                                                                                                                      <  2:610472/250‑1 (MQ=255)
                                  ttACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAAc                                                                                                                                                                                                     >  2:630745/1‑251 (MQ=255)
                                        gcgGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAAtt                                                                                                                                                                                               <  2:152564/251‑1 (MQ=255)
                                        gcgGCAGATAGGTGTTCGGGTCCAGCGTAATATCCGCCCCCCGACCCACAACAACGCCACCAATACGGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAAtt                                                                                                                                                                                               <  2:545234/251‑1 (MQ=255)
                                            cAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTccc                                                                                                                                                                                            >  2:5227/1‑250 (MQ=255)
                                                                     atatCCGACACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAAt                                                                                                                                                                  >  1:481632/1‑251 (MQ=255)
                                                                            ccACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGGTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGccc                                                                                                                                                           <  2:387433/251‑1 (MQ=255)
                                                                                  gACCCACAACAACGCCAACAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAACAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAAc                                                                                                                                                     >  1:467711/1‑251 (MQ=255)
                                                                                     ccACAACAACGCCACCAATACTGGCCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATacccacc                                                                                                                                                                                                                                   <  2:49712/170‑1 (MQ=255)
                                                                                     ccACAACAACGCCACCAATACTGGCCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATacccacc                                                                                                                                                                                                                                   >  1:49712/1‑170 (MQ=255)
                                                                                           caacGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCag                                                                                                                                               >  1:20639/1‑248 (MQ=255)
                                                                                                    caATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGAc                                                                                                                                   <  1:428384/251‑1 (MQ=255)
                                                                                                          tGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATAGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAAAAAACAGCGCCGCTAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAAATCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACggg                                                                                                                             >  1:269284/1‑250 (MQ=255)
                                                                                                           gACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTg                                                                                                                            <  1:396913/251‑1 (MQ=255)
                                                                                                                gagaGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTTGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGt                                                                                                                       >  1:341287/1‑251 (MQ=255)
                                                                                                                            tcacgccgccAAGGTTATCGAACGTCGCATAAAGCGGGTAGGTCGGTTCAGTACGTCCAGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGATAATAAAAAAAAACCCATCAAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACGTCAATGCGGTCAGCACAAAATCATGCCCCAGAACAGCCAGATACGACTGGACACCGAAGCGAGAGGATGCCCGGCt                                                                                                           >  2:460359/5‑251 (MQ=255)
                                                                                                                              aGGCCTCCAATGTTATCGAACGGCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCCCCCAAATTACATTTTTTTTCGTTTACATGAACTCAATTCCCAAACAGCAATGCGGTCAGCACAAAATCCAGCCCCAGAACATCCAGAGACGAGTGTACAACGGTGCGAGTGGTTGCTCGGCTaa                                                                                                         <  2:51620/251‑1 (MQ=255)
                                                                                                                                   gccAATGTTATCGCACGTCGCATCACGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCATTGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCg                                                                                                    >  2:45495/1‑251 (MQ=255)
                                                                                                                                    ccAATGTTATCGAACGTCTCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGTCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCgg                                                                                                   <  2:51286/251‑1 (MQ=255)
                                                                                                                                         gTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGa                                                                                               <  2:36675/250‑1 (MQ=255)
                                                                                                                                               gAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCggg                                                                                        >  1:347339/1‑251 (MQ=255)
                                                                                                                                                   gTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATgg                                                                                    >  2:291590/1‑251 (MQ=255)
                                                                                                                                                                    ggTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAaac                                                                   >  2:389915/1‑251 (MQ=255)
                                                                                                                                                                          ttCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAACTTTCATTTTTTTTCGTTTGCATGCACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTAGTTGCCCGGCTAATCCCGGCAGACGTCGGGCTGGCGTCGTAGCCGTTAACCAACGa                                                              >  2:212056/1‑250 (MQ=255)
                                                                                                                                                                              gTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATcc                                                         >  1:82804/1‑251 (MQ=255)
                                                                                                                                                                                   tATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTc                                                    >  2:20760/1‑251 (MQ=255)
                                                                                                                                                                                         cGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTg                                              >  2:242816/1‑251 (MQ=255)
                                                                                                                                                                                                ttCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCg                                        >  1:207770/1‑250 (MQ=255)
                                                                                                                                                                                                  cgccgcCTTCAGGCAAACAAACAGCGCCGCCAACAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTGTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAACATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTTATGTCGaa                                      <  1:209382/250‑1 (MQ=255)
                                                                                                                                                                                                    ccgccTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATCCCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAacac                                   <  1:170268/251‑1 (MQ=255)
                                                                                                                                                                                                          tCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACg                               <  1:119733/249‑1 (MQ=255)
                                                                                                                                                                                                           cAGGCCAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCGCCCAAATTTCATGTTTTTTCGTTTGCAGGAACTCAATTCCCAAACATAAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGGGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGct                             <  2:63182/250‑1 (MQ=255)
                                                                                                                                                                                                            aGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGctct                           <  2:257269/251‑1 (MQ=255)
                                                                                                                                                                                                            aGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGctct                           >  1:326318/1‑251 (MQ=255)
                                                                                                                                                                                                            aGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTGCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGGTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGctct                           <  1:630288/251‑1 (MQ=255)
                                                                                                                                                                                                              gCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAGATCCACGTCACCGTGATGGCGAACACCACGCTCtt                          >  1:560568/1‑250 (MQ=255)
                                                                                                                                                                                                                   caaacaGCGACGCCAGCAGAGCTGCTAATAAAAACATACCCACCCAAATTTCATTTTTTTTCGTTTACATGAACTCAATTCCCAAACATCAATGCAATCAGCACAAAATCCAGCCCCAGAACAGCCAGAAACGAGTGGACAACGGGGCCAGTGGTTGCCCGCCTAATCCCGGAAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGCTGGCGAACACCACCCTCTTaacca                     >  2:307334/1‑247 (MQ=255)
                                                                                                                                                                                                                         gcgcCGCCAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCTCCGTGATGGCGAACACCACGCTCTTAATCAGACAGtt              <  1:510615/251‑1 (MQ=255)
                                                                                                                                                                                                                            ccgccAGCAGTGCTGCTAATAAAAAAATACGCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACAGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGAc           >  1:427920/1‑251 (MQ=255)
                                                                                                                                                                                                                              gccAGCAGTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCa         <  1:291590/251‑1 (MQ=255)
                                                                                                                                                                                                                                cagcagTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACg                                                               >  1:309742/1‑195 (MQ=255)
                                                                                                                                                                                                                                cagcagTGCTGCTAATAAAAAAATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACg                                                               <  2:309742/195‑1 (MQ=255)
                                                                                                                                                                                                                                     gTGCTGCTAATAAAAAAACACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCAAAAAATCCAGCCCCAGAACAGCCAGAGACGAGTTGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGGTAAACACCGAAATCCACGTCACCGTGATTGCGAACACCACGCTCTTAATCATACAGTTGACCAGATCCAt  <  2:330876/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          
GGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTATGGACGTCACGTTCGCCGCCTTCAGGCAAACAAACAGCGCCGCCAGCAGTGCTGCTAATAAAAAGATACCCACCCAAATTTCATTTTTTTTCGTTTGCATGAACTCAATTCCCAAACATCAATGCGGTCAGCACAAAATCCAGCCCCAGAACAGCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGATCCAT  >  NC_000913/3338181‑3338660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑