Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,560,376	C→T	G102D (GGT→GAT)	yjiH ←	uncharacterized protein YjiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,560,376	0	C	T	100.0%	203.8 / NA	63	G102D (GGT→GAT)	yjiH	uncharacterized protein YjiH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/33);  total (30/33)

AACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAA  >  NC_000913/4560130‑4560607                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        aaCACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGatcatc                                                                                                                                                                                                                                     <  2:518482/251‑1 (MQ=255)     ccGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTa                                                                                                                                                                                                                                 <  2:433783/251‑1 (MQ=255)      cgcgACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTaa                                                                                                                                                                                                                                <  1:408480/251‑1 (MQ=255)         gACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGTCTGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTg                                                                                                                                                                                                                             >  2:497024/1‑251 (MQ=255)         gACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTg                                                                                                                                                                                                                             <  1:102359/251‑1 (MQ=255)         gACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTg                                                                                                                                                                                                                             <  2:344630/251‑1 (MQ=255)           cGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGCAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGa                                                                                                                                                                                                                            >  1:70977/1‑250 (MQ=255)            gCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGaaa                                                                                                                                                                                                                          <  1:81993/251‑1 (MQ=255)             ccGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAaat                                                                                                                                                                                                                         <  1:129435/251‑1 (MQ=255)                 aaGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATc                                                                                                                                                                                                                     <  2:223193/251‑1 (MQ=255)                     gaAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGAGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATcccc                                                                                                                                                                                                                  >  1:6751/1‑250 (MQ=255)                             tGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCGCCAAGATCATCGGTAATTGAAATAATCCCCagagagag                                                                                                                                                                                                          <  2:70977/250‑1 (MQ=255)                             tGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCagagagag                                                                                                                                                                                                          <  1:597903/250‑1 (MQ=255)                               ggtatgatgGCGCTACCGCTGGTTTGATATGCGGCGAAAAGAACTTGATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCTCCAAGATCATCGGTAATTGAAATAATCCCCAAAGAGAGAAt                                                                                                                                                                                                       <  2:300815/248‑1 (MQ=255)                                  atgatgGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAAtgac                                                                                                                                                                                                    >  1:276022/1‑251 (MQ=255)                                       ggCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAAGTTCTCCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCAAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACATGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGTGAGAAtgactgac                                                                                                                                                                                                <  2:331239/250‑1 (MQ=255)                                        gcgcTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACgg                                                                                                                                                                                              <  2:272445/251‑1 (MQ=255)                                                gCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCACGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCt                                                                                                                                                                                      >  1:653392/1‑251 (MQ=255)                                                   ggTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTcc                                                                                                                                                                                    >  2:568356/1‑250 (MQ=255)                                                   ggTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTcc                                                                                                                                                                                    <  1:525768/250‑1 (MQ=255)                                                         atatGCGGCGAAAATCACTTTATCGCGTTCGAACATTTAACCTTCGTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGTGCAGATCCGTGGAATCACCAACAACGGCTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGAAATCCACCAAGATCATCGGTAATTGCAATAATCCTCAGAGAGAGGATGACTGACGGCGCCAGCTCCAgcgcc                                                                                                                                                                              >  1:633381/1‑249 (MQ=255)                                                                     aaTCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCAt                                                                                                                                                                 >  1:108007/1‑251 (MQ=255)                                                                            ttATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAg                                                                                                                                                           <  2:460709/250‑1 (MQ=255)                                                                             tATCGCGTTCGGAAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACAAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGcc                                                                                                                                                         >  2:198409/1‑251 (MQ=255)                                                                                 gcgTTCGGTAATTTCTCCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCcg                                                                                                                                                     <  2:179240/251‑1 (MQ=255)                                                                                      cGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGCGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTCATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGccc                                                                                                                                                >  1:469699/1‑250 (MQ=255)                                                                                          aaTTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACccg                                                                                                                                            >  1:547608/1‑251 (MQ=255)                                                                                              tCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTCGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACccgcgcc                                                                                                                                        >  2:34830/1‑251 (MQ=255)                                                                                              tCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACccgcgcc                                                                                                                                        >  1:439390/1‑251 (MQ=255)                                                                                               cacgttcctcctccAGCTCTCTGGTCATGCCGGGGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCGACAACGGTTTTAGCACAGGTGTCATTAACTGGTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCTAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACccgcgca                                                                                                                                        <  2:7557/238‑2 (MQ=255)                                                                                                     ccTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCa                                                                                                                                                                                                                 <  1:298813/171‑1 (MQ=255)                                                                                                     ccTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCa                                                                                                                                                                                                                 >  2:298813/1‑171 (MQ=255)                                                                                                              gCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGgtggtg                                                                                                                        <  1:34830/251‑1 (MQ=255)                                                                                                                   ttGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGc                                                                                                                      <  2:439390/248‑1 (MQ=255)                                                                                                                        cATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTc                                                                                                               <  2:223141/250‑1 (MQ=255)                                                                                                                        cATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAAGAATCCCCAGAGAGATAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTc                                                                                                               <  1:384513/250‑1 (MQ=255)                                                                                                                        cATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGAGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGCGGTGGCTCCGTTCg                                                                                                              >  2:116270/1‑251 (MQ=255)                                                                                                                          tACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGcc                                                                                                            >  2:206521/1‑250 (MQ=255)                                                                                                                             cggcggcggCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCTCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCa                                                                                                         <  1:188634/251‑1 (MQ=255)                                                                                                                             cggcggcggCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCa                                                                                                         <  2:13735/251‑1 (MQ=255)                                                                                                                                              ttttGCAGGTTAGCGATAAGCGCCAGCGAGCAGATCCGTGGAATGCCCAACAACGGTTTTAGCCCAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCGTCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGAGGCTCCGTTCGCACCAGGCAGTTGCACAAACg                                                                                         >  1:214627/1‑250 (MQ=255)                                                                                                                                                 tGCAGGTTTGGGATCAGCGCAAGCGAGTAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGTCGCGTAATCCACCAAGATCATCGGAAATTGAAATCATCCCCAGCGAGAGAATGACCGACGTGGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGCGGCGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATcc                                                                                     >  2:307403/1‑251 (MQ=255)                                                                                                                                                  gCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCg                                                                                    <  1:250539/251‑1 (MQ=255)                                                                                                                                                          gCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAAc                                                                            >  1:227163/1‑251 (MQ=255)                                                                                                                                                            gATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACgg                                                                          >  1:336129/1‑251 (MQ=255)                                                                                                                                                                      aGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCGAAGAGAAAGCCACCTTTAGCCCCCGCGCCACCCTCTCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCaaa                                                                <  1:74403/251‑1 (MQ=255)                                                                                                                                                                      aGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCAAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAACGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCaa                                                                 >  1:282522/1‑250 (MQ=255)                                                                                                                                                                               aTCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGcc                                                        >  1:577721/1‑250 (MQ=255)                                                                                                                                                                                  ccTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGccacca                                                    <  1:579373/251‑1 (MQ=255)                                                                                                                                                                                     ggAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGaa                                                                        >  2:74056/1‑228 (MQ=255)                                                                                                                                                                                     ggAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGaa                                                                        <  1:74056/228‑1 (MQ=255)                                                                                                                                                                                     ggAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGct                                                 <  2:358955/251‑1 (MQ=255)                                                                                                                                                                                       aaTGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGctgt                                               >  1:367233/1‑251 (MQ=255)                                                                                                                                                                                        aTGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCTCCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGctgtc                                              <  2:336129/251‑1 (MQ=255)                                                                                                                                                                                                          aGCACAGGTGTCATTAACTGCTGCGCGGCCCGTATACCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGATATAATGACTGACGGTTCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCCCCCGCGCCGCGAAACGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACAcc                            <  1:558095/251‑1 (MQ=255)                                                                                                                                                                                                               aGGTGTCATTAACTGCTGCGCGCCGCGTAATCCACCAAGATCATCGGTAATTGACATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGACAGCCATCTTTAGCCCCCGCGCCACACGCGCCGCGACATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTCCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGCCTGTTCCGCAAAACACACCGGaa                        >  1:417123/1‑250 (MQ=255)                                                                                                                                                                                                                  tgtCATTAACTGCTGCGCGGAGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGACAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAACTGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAACAACCGGaaaaa                     >  2:555440/1‑250 (MQ=255)                                                                                                                                                                                                                      attaacttcgttgcggcgcgTAAACCACCAAGTTCATCGTTAATTGAAAAAATCACCAGAGATAGAATGACTGACGGTGCCAGCTCCAGCGCAAAAATAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCAAAACGATCCGTTCAGAACGGAAAAAACAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGTAAAAAAGAt                <  2:7253/239‑1 (MQ=255)                                                                                                                                                                                                                       ttAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGACAAATCACAAACGCGCCCCCAGCTGTCTGTTCCGGCACAAACACCGGAAAAAAAGAtt               >  1:208641/1‑251 (MQ=255)                                                                                                                                                                                                                        tAACTGCTGCGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGAtt               <  1:568356/250‑1 (MQ=255)                                                                                                                                                                                                                               tgcGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACg                                                                                                                                                                                               >  2:448505/1‑67 (MQ=255)                                                                                                                                                                                                                               tgcGCGGCGCGTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACg                                                                                                                                                                                               <  1:448505/67‑1 (MQ=255)                                                                                                                                                                                                                                    ggcgcgTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGaaaa   >  1:388183/1‑250 (MQ=255)                                                                                                                                                                                                                                     gcgcgTAATCCACCAAGATCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGAAAAGAGAAAGCCATCTTTAGCCCCCGCGACACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGCACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGGGaaaaa  >  1:464255/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        AACACCGCGACGCCGGAAGAGAAGTAGTTGGTGATGATGGCGCTACCGCTGGTTTGATATGCGGCGAAAATCACTTTATCGCGTTCGGTAATTTCACCTTCCTGCGCCAGCTCTTTGGTCATACCGGCGGCGGCATCGGTGTTTTGCAGGTTAGCGATCAGCGCCAGCGAGCAGATCCCTGGAATGCCCAACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGGCGCGTAATCCACCAAGACCATCGGTAATTGAAATAATCCCCAGAGAGAGAATGACTGACGGTGCCAGCTCCAGCGCAAAGAGAAAGCCATCTTTAGCCCCCGCGCCACCCGCGCCGCGAAATGAGGTGGTGGCTCCGTTCGCACCAGGCAGTTGCCCAAACGATCCGTTCAGAACGGAAAAATCAAAAACGCGCCACCAGCTGTCTGTTCCGGAAAAAACACCGGAAAAAAAGATTATGGTGAGAAAAA  >  NC_000913/4560130‑4560607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑