Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,048,632	C→T	S556F (TCT→TTT)	polA →	DNA polymerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,048,632	0	C	T	100.0%	210.4 / NA	64	S556F (TCT→TTT)	polA	DNA polymerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/35);  total (29/35)

TGCTGGTAAAGGCAAAAATCAACTGACCTTTAACCAGATTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTCTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCG  >  NC_000913/4048393‑4048867                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            tGCTGGTAAAGGCAAAAATCAACTGACCTTTAACCAGATTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTc                                                                                                                                                                                                                                   <  2:456600/250‑1 (MQ=255)     ggTAAAGGCAAAAATCAACTGACCTTTAACCAGATTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTTCACAATCATTTTGAAGAGCTCAccc                                                                                                                                                                                                                               <  2:102462/250‑1 (MQ=255)      gTAAAGGCAAAAATCAACTGACCTTTAACCAGATTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACTTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCtt                                                                                                                                                                                                                             <  2:424182/251‑1 (MQ=255)       tAAAGGCAAAAATCAACTGACCTTTAACCAGATTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTc                                                                                                                                                                                                                            >  1:17370/1‑251 (MQ=255)        aaaGGCAAAAATCAACTGACCTTTAACCAGATTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCg                                                                                                                                                                                                                           <  1:658675/251‑1 (MQ=255)        aaaGGCAAAAATCAACTGACCTTTAACCAGATTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGAAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCg                                                                                                                                                                                                                           >  2:546963/1‑251 (MQ=255)             cAAAAATCAACTGACCTTTAACCAGATTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAAAGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTgg                                                                                                                                                                                                                      >  1:250364/1‑251 (MQ=255)                 aaTCAACTGATCTTGAACCAGATTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATATGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGGTCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCGCCCTTCGTCTGGCTGa                                                                                                                                                                                                                  <  1:644372/251‑1 (MQ=255)                   tCAACTGACCTTTAACCAGATTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGc                                                                                                                                                                                                                >  1:243647/1‑251 (MQ=255)                    cAACTGACCTTTAACCAGATTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCGTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGc                                                                                                                                                                                                                <  2:124922/250‑1 (MQ=255)                         gACCTTTAACCATAGTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGa                                                                                                                                                                                                            <  1:148920/249‑1 (MQ=255)                                   cAGATTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAgcgc                                                                                                                                                                                                 >  1:108368/1‑250 (MQ=255)                                         gCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAAt                                                                                                                                                                                          <  2:267700/251‑1 (MQ=255)                                           ccTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTg                                                                                                                                                                                        >  1:370869/1‑251 (MQ=255)                                               gaagaaGACGGACGTTACGCCGCAGCAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAACAACACAAAGGGCCGTTGACCGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAgg                                                                                                                                                                                    >  2:267090/1‑251 (MQ=255)                                                            gTTACGCCGACGAAGATGCAGATGTCACCTTGCAGTTGCATGTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGGTGAACGTCTTCGAGAATATCGAAATGCCGATGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCTTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAAc                                                                                                                                                                       <  2:189581/251‑1 (MQ=255)                                                                cgccgcCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAAGTGAGGAATTTAACCttt                                                                                                                                                                   >  2:661313/1‑251 (MQ=255)                                                                   cgccgAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGGAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTttctt                                                                                                                                                                <  1:81784/251‑1 (MQ=255)                                                                    gccgAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTttcttc                                                                                                                                                               <  1:235245/251‑1 (MQ=255)                                                                    gccgAAGATGCAGATGTCACCTTGCAGATGCATCTGAACATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAAAGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTACAGGTGAGGAATTTAACCTttcttc                                                                                                                                                               >  2:414609/1‑251 (MQ=255)                                                                    gccgAAGATGCAGATGTCAACTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGATGGAGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTttcttc                                                                                                                                                               >  2:99871/1‑251 (MQ=255)                                                                      cgAAGATGAAGATGACACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGCAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCCGGTGAGGAATTTAACCTttctttc                                                                                                                                                              >  2:194915/1‑248 (MQ=255)                                                                       gAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTccac                                                                                                                                                            <  2:243647/251‑1 (MQ=255)                                                                       gAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGGCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTccac                                                                                                                                                            <  1:303802/251‑1 (MQ=255)                                                                         aGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCCCCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCa                                                                                                                                                                                      <  1:555850/223‑1 (MQ=255)                                                                         aGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCa                                                                                                                                                                                      >  2:555850/1‑223 (MQ=255)                                                                            tGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGc                                                                                                                                                       >  1:494372/1‑251 (MQ=255)                                                                                  tgtaacgttGCAGTTGAATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGACGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGCGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGTAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGACTGAAATTGCAGGTGAGGAATTTAACCTTTCTTACACCAAGCAGTTAc                                                                                                                                                 >  2:393256/8‑251 (MQ=255)                                                                                   gTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGG‑CCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACaa                                                                                                                                               <  2:117704/251‑1 (MQ=255)                                                                                           gCAGTTGCATCTGACAATGTGGCCGGAACTGCAAAAACACAAAGAGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCAttt                                                                                                                                        >  1:495597/1‑250 (MQ=255)                                                                                            cAGTTGCATCTGAAAATGTGGGCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCGTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATtct                                                                                                                                       <  2:293019/251‑1 (MQ=255)                                                                                              gTTGCATCTGAAAATGTGGCCGGCTCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAGGATCGATCCGAAAGAGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGAGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTctct                                                                                                                                     >  1:309677/1‑251 (MQ=255)                                                                                                        aaaaTGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACa                                                                                                                           <  1:6105/251‑1 (MQ=255)                                                                                                           aTGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAggg                                                                                                                        <  1:382495/251‑1 (MQ=255)                                                                                                                   ggATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAAc                                                                                                                >  1:297345/1‑251 (MQ=255)                                                                                                                         gCANAAACACAAAGGGCCGGTGAATGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTGTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTga                                                                                                          <  2:607214/251‑1 (MQ=255)                                                                                                                         gCAAAAACACAAAAGGCCGTTGAACGTCTGCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAACAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATGAAACCga                                                                                                             <  2:250364/248‑2 (MQ=255)                                                                                                                             aaaCACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTgaaga                                                                                                       >  1:455741/1‑250 (MQ=255)                                                                                                                              aaCACAAAGGGCCGTTGAACGTCTTCGAGAAGATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGGGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCGCCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCACTAAACCGCTGTAGaaa                                                                                                     <  2:222016/251‑1 (MQ=255)                                                                                                                                     aGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCgg                                                                                              >  1:377810/1‑251 (MQ=255)                                                                                                                                          cGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGgc                                                                                         >  2:61433/1‑251 (MQ=255)                                                                                                                                              gaccGTCTTCGAGAATATCGAACTGCCGCTGTTGCCGGTGCTTTCACTCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCCTTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAACACAGGGCATTAAACCGCTGAAGAAACCGCCTGGTGgcgcgc                                                                                     >  2:277701/4‑251 (MQ=255)                                                                                                                                                aCGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAACGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCg                                                                                   >  2:275982/1‑251 (MQ=255)                                                                                                                                                      tCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAAc                                                                              <  2:12145/250‑1 (MQ=255)                                                                                                                                                       cgcgaATATCGAAATGCCGCTGGTGCCGGTGCGTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGTCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGAGCATTAAACTGCTGAAGAAAACGCCGGGTGGCGCGCCTTCAActt                                                                            <  2:453772/248‑3 (MQ=255)                                                                                                                                                               tCGAACTGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCAGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTCGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAag                                                                      >  2:41059/1‑249 (MQ=255)                                                                                                                                                                    aTGCCGCTGGTGCCGGTACTTTCACGCATTGAACGTAACGTGGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGTCTGAGCTGTAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTATCCTTTCTTCCTCCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCCTTAAACCGCTGAAGAAAACGCCGGGTGTCGTGCCGTCAACGTCGGAATATGTACt                                                               <  2:364790/251‑1 (MQ=255)                                                                                                                                                                           gggTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGgaagaa                                                        <  2:487591/250‑1 (MQ=255)                                                                                                                                                                               gCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTg                                                     <  2:108368/250‑1 (MQ=255)                                                                                                                                                                                  ggTGCTTTCAAGCATTGAACGTAACGATGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAGGCGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGACATCGAAGGTGAGGAATTTAACCTTTCTTCCCGCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGATGAAGAAAACGCCGGGAGGCGCGCCGTCAACGTCGGAGGAGGTCCTGGACGAACTGgcgc                                                 >  1:609533/1‑251 (MQ=255)                                                                                                                                                                                             ccATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATGTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAATAAAGCGCATGAAATTGCAGGTGATGAATTTAACCTTTCTGCCACCGAGCAGTTACAATTCATTCTTTTTGAAAAACAGGGCATTATACCGCTGAATAAAACGCCGGGTGGCGCTACGTCAACGCCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCg                                      <  1:391138/250‑1 (MQ=255)                                                                                                                                                                                                          cGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGa                         <  1:574123/251‑1 (MQ=255)                                                                                                                                                                                                           ggTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGAt                        <  1:321974/251‑1 (MQ=255)                                                                                                                                                                                                              gtgAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCt                     <  2:464544/251‑1 (MQ=255)                                                                                                                                                                                                                gAAGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCCAAAGTGATTCTga                   <  2:462695/251‑2 (MQ=255)                                                                                                                                                                                                                  aGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAAc                                                                                                                >  1:45873/1‑156 (MQ=255)                                                                                                                                                                                                                  aGATCGATCCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAAc                                                                                                                <  2:45873/156‑1 (MQ=255)                                                                                                                                                                                                                      cgatcCGAAAGTGCTTCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATc             <  2:459080/251‑1 (MQ=255)                                                                                                                                                                                                                      cgatcCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATc             <  2:480313/251‑1 (MQ=255)                                                                                                                                                                                                                      caatacGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTCGAAAACAAAGCGCATGAAATTGCAGGAGCGGAATTTAACCGTTCTTCCACCAAGCAGGTACAAACCATTAGCTTTGAAACATAGGGCATTAAACCGCTGAAGAAACCGCCGGGTGGCGCGCCGACAACGTCGGAAGAGGCACTGGAAGAACTGGCGCTGTGCTATCCGTTGCCAAAAGTGATTCTGGAGAATc             >  2:76115/6‑251 (MQ=255)                                                                                                                                                                                                                        atcCGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGt           >  1:122172/1‑251 (MQ=255)                                                                                                                                                                                                                           cGAAAGTGCTGCACAATCATTTTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGt        <  1:352838/251‑1 (MQ=255)                                                                                                                                                                                                                                 tgctgcACAATCATTTTGAAGAGCTCACCCTTCGTCTTGCTGCGCTCCATCAGTACGCGCATGAAATCCGAGGAAATGAATTTCACCTTTCTTCCACCAAGTCGTTCCAAACCATTCTCCTTCAAAAACAAAGCATTAAACCACTGAAGAAAACTCCGTGTAACGCGTCGTCAACGTCATAAGCGGCACTGGAAGAACTGGCGCTGGACTATCCGTTGCCACAAGCAATTCTGGAATATCGCGCTCTGGc   >  1:80069/1‑250 (MQ=255)                                                                                                                                                                                                                                 tgctgcACAATCATTTTGAAGAGCTCACCCTTCGTATGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCg  >  1:171764/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            TGCTGGTAAAGGCAAAAATCAACTGACCTTTAACCAGATTGCCCTCGAAGAAGCCGGACGTTACGCCGCCGAAGATGCAGATGTCACCTTGCAGTTGCATCTGAAAATGTGGCCGGATCTGCAAAAACACAAAGGGCCGTTGAACGTCTTCGAGAATATCGAAATGCCGCTGGTGCCGGTGCTTTCACGCATTGAACGTAACGGTGTGAAGATCGATCCGAAAGTGCTGCACAATCATTCTGAAGAGCTCACCCTTCGTCTGGCTGAGCTGGAAAAGAAAGCGCATGAAATTGCAGGTGAGGAATTTAACCTTTCTTCCACCAAGCAGTTACAAACCATTCTCTTTGAAAAACAGGGCATTAAACCGCTGAAGAAAACGCCGGGTGGCGCGCCGTCAACGTCGGAAGAGGTACTGGAAGAACTGGCGCTGGACTATCCGTTGCCAAAAGTGATTCTGGAGTATCGTGGTCTGGCG  >  NC_000913/4048393‑4048867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑