Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,890,220	G→A	L105L (CTG→TTG)	yeaY ←	Slp family lipoprotein YeaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,890,220	0	G	A	100.0%	136.8 / NA	41	L105L (CTG→TTG)	yeaY	Slp family lipoprotein YeaY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/23);  total (18/23)

CGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCC  >  NC_000913/1889986‑1890452                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         cGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCCGTTGTCCACGGAAATCCACCGGGTCCAAAAAACTGTTCACATct                                                                                                                                                                                                                          >  2:142791/1‑250 (MQ=255)     aTTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCAt                                                                                                                                                                                                                      >  1:629298/1‑251 (MQ=255)                          gTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGtt                                                                                                                                                                                                 >  2:527564/1‑251 (MQ=255)                              ccATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTcc                                                                                                                                                                                             >  1:589067/1‑251 (MQ=255)                                  ggcgccagcccctgccGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGc                                                                                                                                                                                         <  1:366930/237‑1 (MQ=255)                                      ccgccccgaaCCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCg                                                                                                                                                                                     <  1:143679/242‑1 (MQ=255)                                           caCGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTg                                                                                                                                                                                <  2:329261/251‑1 (MQ=255)                                                                   cAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGcggtacggt                                                                                                                                                         <  2:381767/250‑1 (MQ=255)                                                                   cAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATGGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGATTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGcggtacggt                                                                                                                                                         <  1:651741/250‑1 (MQ=255)                                                                     aTCGGCTGAGGCGGCATAATCACCTGCTGGGTTCAATGCCCACGTTTGTAACCCGTTACTTGCATCCCCATAAATTTATAGGACGTATTGCCGATTTTGCCGTCAACCGCACCAGTGAATGACCCGGCTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAGAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCGCCCAGCGTCGGGCTGGCTCCGCTGTCCAGCGGTACGGTAGc                                                                                                                                                      >  1:609984/1‑251 (MQ=255)                                                                     aTCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAAAGTCTGTATCCCGTTACGTGCATCACCATAAATGTAGAGGTCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAGCCAGTTGTCCACGGAAATCCACCGCGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGATGGTTCGCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGc                                                                                                                                                      <  1:26337/251‑1 (MQ=255)                                                                             aGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCa                                                                                                                                              <  2:159104/251‑1 (MQ=255)                                                                                cggcATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGc                                                                                                                                           >  2:11995/1‑251 (MQ=255)                                                                                    aTAATCACCGGCTGGGTGAAATGCCAACGTTTGTAATTCGTTACTTGCATCACCATAAATTTATAGGGCGTACTGTCGATTTTGCCGTCAACCGCACCAGTGATTGGCACTACTACCGTAACCAGTTGTCCACGGAAATCCACAGCTTCCAAAAAACCTTTCTCAGCGGCATAATTGCGACCGCGAGAAGGTGCTCCCAGCGTCGGTCGTGCTTCGGTGTCCAGCGGTATGGTAGCAATGTCCAGGCTAGt                                                                                                                                       <  2:27058/251‑1 (MQ=255)                                                                                       aTCACCTGCTGGGTTAACTGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGCTTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATg                                                                                                                                                                                                                 >  2:287192/1‑174 (MQ=255)                                                                                       aTCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATg                                                                                                                                                                                                                 <  1:287192/174‑1 (MQ=255)                                                                                         cACCTGCTGGGTTAAAGGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTcc                                                                                                                                  <  2:390269/251‑1 (MQ=255)                                                                                          aCCTGCTGGGTTAAATGCCAACTTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGTGCGTATTGCCGATTTGGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCTCCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGATAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTCCGGTAGCAATTTCCAGGCGAGTTTTccc                                                                                                                                 <  1:619687/251‑1 (MQ=255)                                                                                              gctgGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTg                                                                                                                              <  1:560683/250‑1 (MQ=255)                                                                                                          gCNAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGGCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACGGCGAGAAGGTTTTCCCAGCGTCGGTCTGGCTCCGCGGTCGAGCGGTACGGGAGCAATTTCCAGGCGAGTTTTCCCTCGCTGGATGTGTAc                                                                                                                  <  2:593571/250‑1 (MQ=255)                                                                                                                          cGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGccac                                                                                                 >  2:539762/1‑251 (MQ=255)                                                                                                                            ttACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGccacc                                                                                                >  2:161326/1‑250 (MQ=255)                                                                                                                                   cATCACCATAAATTTATAGGGCGTCTTGCCGATTTTGCCGTCAACCGCAACAGTGATTGGCCCGACTACAGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGACCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTg                                                                                        >  2:616288/1‑251 (MQ=255)                                                                                                                                       cccATAAATTTATAGGGCTTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGTGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCTAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGcctc                                                                                    <  1:90404/250‑1 (MQ=255)                                                                                                                                        ccATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGcctcc                                                                                   >  2:51281/1‑251 (MQ=255)                                                                                                                                         cATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGcctcct                                                                                  >  2:143057/1‑251 (MQ=255)                                                                                                                                               tttATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGACCa                                                                             >  1:550769/1‑250 (MQ=255)                                                                                                                                                         gTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCAACTACCGTAACCAGTTGTCCACGGAAATCCTCCGAGTACAAAAAACCGTTCTCATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTATGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTg                                                                  <  1:332364/251‑1 (MQ=255)                                                                                                                                                           aTTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTgcg                                                                <  1:477943/251‑1 (MQ=255)                                                                                                                                                             tGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTATCAATTTCCAGGCGAGttt                                                                                                                                     <  1:521459/180‑1 (MQ=255)                                                                                                                                                             tGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGttt                                                                                                                                     >  2:521459/1‑180 (MQ=255)                                                                                                                                                                        ccGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCAc                                                   <  2:436579/251‑1 (MQ=255)                                                                                                                                                                                    ccAGAGATTAGCCCGACTCCCGAAACAAGTTGTCCAAGGAAAACCCACAGGTCCAAACAACCGTTCACATCGGCATAAATGCAACCGCGAGAAGGGTCTCCCAGCGCCGGTCTGGCTCCGCTATCAAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTCACGCAACAACTTTGCCACCAAACCGTGCCTCCTCACCAACGTACACCTGCGACGCACTCATCACCCGACCTACATc                                       >  2:635552/1‑251 (MQ=255)                                                                                                                                                                                                 cGANTACCGTAACCAGTTTTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACGCATCTCCCGAACTAAATCTTGTTGCGGCGTg                          <  2:586429/251‑1 (MQ=255)                                                                                                                                                                                                      aCCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGAct                     <  2:550769/251‑1 (MQ=255)                                                                                                                                                                                                       ccGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGc                                                                                                                                                                 <  2:183048/110‑1 (MQ=255)                                                                                                                                                                                                       ccGTAACAAGTTGTCAACGGAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGc                                                                                                                                                                 >  1:183048/1‑110 (MQ=255)                                                                                                                                                                                                            aCCAGTTGTGCAAGGAACTCCAACGGGCCCAAAAAACCGCTCCCAACGGCATAAATGAGACTGCGAGAAGGTTCTCCAATCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCCGGCCAGTTTTCCCTTGCTGGTTTTGTCCCGCAACCACTCTGCCACCACAGCGTGCCTCCTGACCAACGTCCAGCTGCGGCGCACTCATCACCCGAACTAAAGATTGTTGCGGCGTGGCACTGCTGcc               <  2:351380/251‑1 (MQ=255)                                                                                                                                                                                                                         ggAAATCCACCTGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTGTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTcc  <  2:7463/251‑1 (MQ=255)                                                                                                                                                                                                                         ggAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTCATGGCGTcc  >  1:7463/1‑251 (MQ=255)                                                                                                                                                                                                                         ggAAATCCACCGGGTCCAAAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTcc  >  2:195127/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                         CGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGACCAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCC  >  NC_000913/1889986‑1890452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑