Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,099,992	G→A	S246F (TCC→TTC)	ansB ←	L‑asparaginase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,099,992	0	G	A	99.0%	333.0 / NA	100	S246F (TCC→TTC)	ansB	L‑asparaginase 2
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (49/49);  total (51/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTG  >  NC_000913/3099748‑3100237                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      cagAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAACCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCCGCGCTCACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCgg                                                                                                                                                                                                                                                 >  2:334126/1‑250 (MQ=255)     aCGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAACCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGc                                                                                                                                                                                                                                             <  2:472569/251‑1 (MQ=255)        cgcgcTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAACCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGCGCTTTAGCCGGAAGATCGAATGCGTTAGCGt                                                                                                                                                                                                                                           >  2:447547/1‑250 (MQ=255)           gcTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAACCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGtt                                                                                                                                                                                                                                       <  2:505202/251‑1 (MQ=255)              ttttGCGGTTTCAGCGTGCCAGAGGCGACGAAACCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACCCCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTta                                                                                                                                                                                                                                      <  1:124012/249‑1 (MQ=255)                ttGCGGGTTCAGCGTGCCAGAGGCGACGAAACCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATaa                                                                                                                                                                                                                                   >  1:482637/1‑250 (MQ=255)                   cGGGTTCTGCGTGCCAGAGTCGACGAATCCGTATGTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTTTCGAACACAGATTTATTCAGGTTGCCGTTACCCACACCATCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGGTCGAATGCGTTAGCGTAGTTATAAACaa                                                                                                                                                                                                                               <  1:409278/251‑1 (MQ=255)                       ttCAGCGTGCCAGAGGCGACGAAACCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGCTGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGAGTAGTTATAAACAATGcc                                                                                                                                                                                                                           >  1:472074/1‑251 (MQ=255)                         cgncGTGCCAGAGGCGACGAAACCGTATTTCGCATAATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGGTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCg                                                                                                                                                                                                                          <  2:594412/247‑1 (MQ=255)                               gCCAGAGGCGACGAAACCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTc                                                                                                                                                                                                                    <  2:468587/250‑1 (MQ=255)                                        gacgaAACCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTTCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATCTATACAGGTTGCCGTTACCCACGCCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCCGttcat                                                                                                                                                                                                          <  2:474197/251‑1 (MQ=255)                                           gaANCCGTATTTCGCATCATCCACTTCGTCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAACATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGttcattca                                                                                                                                                                                                       <  2:587165/251‑1 (MQ=255)                                             aaccGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGc                                                                                                                                                                                                     <  1:457433/249‑1 (MQ=255)                                              accGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGGCGAACACAGATTTTTACAGGTTGCCGTTACCCCCCCCCGCGATAACGATGCCATCATAGACCGCATCTACCAACGCTTTAGCCGGGAGATCGAAAGCGTTAGCGTAGTTATAAACAATGGCGACTTTCGGGAGttccttccact                                                                                                                                                                                                    >  2:229266/2‑247 (MQ=255)                                               ccGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTTTACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCCTCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTTGTTATAAACAATGCCGCCTTTCGGCAGATCATTCAGCtt                                                                                                                                                                                                   >  2:236021/1‑251 (MQ=255)                                               ccGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGAACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTAGCCAGCGTGTCGAACACCAAATTATACAGGTTGCCGGTACCCACAACAACGCTAACGATGCCCTCATAACCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAAAGCGTTAGCGTAGGTATAAACAATGCCGCCTTTCGGCAGTTCATTCAGCtt                                                                                                                                                                                                   >  1:116704/1‑251 (MQ=255)                                                    tttCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGAGCTAACGATGCCATCATAGCCCCCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGCTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTag                                                                                                                                                                                                 >  2:294502/1‑248 (MQ=255)                                                         catcatCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTGCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCg                                                                                                                                                                                         <  1:373880/251‑1 (MQ=255)                                                                cACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCg                                                                                                                                                                                  >  1:224487/1‑251 (MQ=255)                                                                cACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCg                                                                                                                                                                                  >  1:322030/1‑251 (MQ=255)                                                                  cTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAAATCGAATGCGTTAGCGTAGTTATAACCAATGCCGACTTTCGGCAGTTCATTCCGCTTAGAGACATCGCATGGctcg                                                                                                                                                                                >  2:423099/1‑248 (MQ=255)                                                                    tCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCgtgt                                                                                                                                                                               <  2:482637/250‑1 (MQ=255)                                                                    tCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCgtg                                                                                                                                                                                <  1:2123/249‑1 (MQ=255)                                                                        cATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTg                                                                                                                                                                          <  1:210592/251‑1 (MQ=255)                                                                             tGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCAACTACCCGTGCTTTAGCCGGAAGATAGAATGCGTTAGCGTAGTTATAAAAAATGCCGACTTTCGGCAGTTAATTCAGCTTAAAGACATCGAATGGCGTGTCGCTGGTATg                                                                                                                                                                     >  1:391247/1‑251 (MQ=255)                                                                                   gTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACg                                                                                                                                                               <  1:229266/251‑1 (MQ=255)                                                                                        gcCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCg                                                                                                                                                          >  1:395322/1‑251 (MQ=255)                                                                                           cGTCGGTACTCTGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCATTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCgggg                                                                                                                                                       <  2:642203/251‑1 (MQ=255)                                                                                              cGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACCGGTTGCCGTTACCCACACCAGCGCTAACGATGCCCTCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGGCATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTAc                                                                                                                                                    >  1:616117/1‑251 (MQ=255)                                                                                               gggaCGCGGGACGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGCCGCCAGCGGGTCGCACACAGATTTATACACGCTGCCATTAACCCCACCAGCGCCACAGATACCATCATAGCCCGCATCTGCCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCTGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGAATGCTTACGTGCCGGGGTACg                                                                                                                                                   >  2:54448/4‑251 (MQ=255)                                                                                                  aCGCGGGAAGAACGCACGACTGCAGTACCGGTGTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGTCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGCCATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCt                                                                                                                                                 <  2:119719/250‑1 (MQ=255)                                                                                                     ggggAAGAATGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTa                                                                                                                                             <  2:273071/250‑1 (MQ=255)                                                                                                      gggAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCCGCGCTAACGATGCCATCATAGCCCGCATCTACCCGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTTGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGt                                                                                                                                              >  2:222320/1‑249 (MQ=255)                                                                                                      gggAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAg                                                                                                                                            <  1:187873/251‑1 (MQ=255)                                                                                                         aagaaCGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCa                                                                                                                                         <  1:447547/251‑1 (MQ=255)                                                                                                           gaaCGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAAt                                                                                                                                       <  2:115774/251‑1 (MQ=255)                                                                                                                       gCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTtg                                                                                                                             <  1:230974/249‑1 (MQ=255)                                                                                                                                gTTTTCGCGGCGGGCGCCCGCGTGTCGAACCCAGCTTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATTCCATCATAGCCCGCCTCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACCATGCCGACTTTCGGCAGTTCATTTAGCTTAGAGACATCGAATTGCGTGTCGCTGGCATGCTTACGTGCCGGGGTACGCTGGGTGTCAATCTTACCGTTGTGAATGTAAcc                                                                                                                  >  2:135906/1‑251 (MQ=255)                                                                                                                                   ttCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCa                                                                                                                <  2:72174/250‑1 (MQ=255)                                                                                                                                      gcggcgGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAgg                                                                                                            <  2:287568/251‑1 (MQ=255)                                                                                                                                       cggcggTCGCCAGCTTGTCGAACACAGATTTATACAGGGTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGGTGTCAATATTACCGTTGGGAATGAAACCCAGAgg                                                                                                            >  2:490147/1‑250 (MQ=255)                                                                                                                                       cggcggTCGCCAGAGTGTCGAACAAAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCAACCAGTGCTTTCGCCGGAAGATCGAATGCGTTAGCGAAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTAAGCTGGAAGTCAATCTTACTGTTTTGAATGTAACCCaga                                                                                                              >  2:552549/1‑248 (MQ=255)                                                                                                                                              cGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAg                                                                                                     <  2:648073/250‑1 (MQ=255)                                                                                                                                              cGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGt                                                                                                    >  1:534671/1‑251 (MQ=255)                                                                                                                                                 cAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGGTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCa                                                                                                                                         <  1:344650/211‑1 (MQ=255)                                                                                                                                                 cAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCa                                                                                                                                         >  2:344650/1‑211 (MQ=255)                                                                                                                                                  aGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAAc                                                                                                >  1:573125/1‑251 (MQ=255)                                                                                                                                                    cGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAgg                                                                                                            <  1:21803/237‑1 (MQ=255)                                                                                                                                                    cGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAgg                                                                                                            >  2:21803/1‑237 (MQ=255)                                                                                                                                                    cGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAAc                                                                                                >  1:656561/1‑249 (MQ=255)                                                                                                                                                       gtCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATTTAACCCAGAGGACCGTAGTTAACAGACt                                                                                           >  1:115152/1‑251 (MQ=255)                                                                                                                                                         cGAACCCAGATTTATGCAGCTTGCCGTTACCCACACCAGCGCTCACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTAATTCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTg                                                                                         >  2:316511/1‑251 (MQ=255)                                                                                                                                                         cGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTg                                                                                         >  1:313934/1‑251 (MQ=255)                                                                                                                                                           aaCACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGa                                                                                        >  1:310757/1‑250 (MQ=255)                                                                                                                                                            acacAGCTTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTAAGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGa                                                                                        >  1:154284/1‑249 (MQ=255)                                                                                                                                                            acacAGATTTATCCAGGCTGCAGTTACCAACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGAACGTAGTTAACAGACTTGa                                                                                        >  2:285834/1‑249 (MQ=255)                                                                                                                                                             cacaGATCTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTTCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAgg                                                                                     <  1:54448/251‑1 (MQ=255)                                                                                                                                                              acaGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCCTCTACCAGAGCTTTAGCCGGAAGATCGCATGCGTTAGCGTAGTTATAAACAATGCCGTCTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACCGACtttagggg                                                                                    >  2:243482/1‑245 (MQ=255)                                                                                                                                                               caGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTc                                                                                   <  2:34648/251‑1 (MQ=255)                                                                                                                                                                  aTTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCt                                                                                >  2:193983/1‑251 (MQ=255)                                                                                                                                                                     tataCAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGAGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGATTAGAGACATCGAATGGCGTGTCGCTGGTCTGCTTACGTGCCGGGGTACGCTGGTAGTCACTCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACg                                                                             >  1:426624/1‑251 (MQ=255)                                                                                                                                                                      ataCAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCACTCTTACCGTTGTGAATGTAACCCAGGGGACCGTAGTTAACAGACTTGAAGGTCGCTACGt                                                                            >  1:66192/1‑251 (MQ=255)                                                                                                                                                                      ataCAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACg                                                                             <  1:207452/250‑1 (MQ=255)                                                                                                                                                                      ataCAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGt                                                                            >  2:6509/1‑251 (MQ=255)                                                                                                                                                                           ggTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCggtg                                                                       >  1:557228/1‑251 (MQ=255)                                                                                                                                                                                    tACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGt                                                               <  2:231613/250‑1 (MQ=255)                                                                                                                                                                                       ccACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTg                                                           <  1:490147/251‑1 (MQ=255)                                                                                                                                                                                         acacCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCAGGAAGATCGAATGCGTTAGCGTAGTTATAAACAACGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTTAATCTTACAGTTGTGAATGTAACCCAGCGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGt                                                         >  1:418331/1‑251 (MQ=255)                                                                                                                                                                                            ccAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGAc                                                      <  1:222320/251‑1 (MQ=255)                                                                                                                                                                                            ccAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGATTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGAc                                                      >  1:559210/1‑251 (MQ=255)                                                                                                                                                                                              aGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTGCGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGt                                                    <  1:274642/251‑1 (MQ=255)                                                                                                                                                                                                   tAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACgg                                               >  2:493676/1‑251 (MQ=255)                                                                                                                                                                                                       gATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCAt                                           <  2:391247/251‑1 (MQ=255)                                                                                                                                                                                                       gATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCAt                                           <  2:322030/251‑1 (MQ=255)                                                                                                                                                                                                           ccATCGTAGCCTGCATCTACAAGTGCTTTAGCCGGAAGCTCGGATGCGATCGCGAAGTTCTAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACCTCGAATGGCGTGTCGCTGGTCTGCTTACGTGCCGGGGAACGCTGGTAGTCAATCTTTCCGTTGTGAATGCAAACCAGAGGACCGGAGTTCACAGACTTGAAGGTCGCTACGACGGTGGTGTTGGTTGTGGTGACgga                                                   >  2:595696/1‑237 (MQ=255)                                                                                                                                                                                                           ccATCATAGCCCGCATCTTCCAGTGCTTTAGCTGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTAGGTTTACGTGGCGGGGTTCGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACGGTAGGTATGAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTc                                                   <  1:595696/239‑1 (MQ=255)                                                                                                                                                                                                            catcatAGCCCGATTCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTATCGTAGTTATAAACAAGGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTGCCATTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTTCGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCAATCAAGc                                      <  2:508899/251‑1 (MQ=255)                                                                                                                                                                                                               catAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAATCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTcgctgctgtcaagcac                                    <  2:616117/250‑16 (MQ=255)                                                                                                                                                                                                               catAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCAc                                    <  2:529874/250‑1 (MQ=255)                                                                                                                                                                                                                 tAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACggg                                 >  2:233068/1‑250 (MQ=255)                                                                                                                                                                                                                   gCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTc                                                   >  2:11732/1‑231 (MQ=255)                                                                                                                                                                                                                   gCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTc                                                   <  1:11732/231‑1 (MQ=255)                                                                                                                                                                                                                     ccGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGtc                              >  2:559531/1‑250 (MQ=255)                                                                                                                                                                                                                      cGCATCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGtcat                            <  2:443384/251‑1 (MQ=255)                                                                                                                                                                                                                         aTCTACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGtcattc                          >  1:592772/1‑250 (MQ=255)                                                                                                                                                                                                                            tACCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACACTGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGAACGCTGGTCGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGAAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATtcatc                       >  1:588507/1‑250 (MQ=255)                                                                                                                                                                                                                            tACCAGAGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTCGTATGCTTACGTGCCGGGGAACGCTGGTAGTCACTCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGTTGGTGTTGGTTTTGATGACGTCACGGCCATCAAGCACGGTGtcattcctc                       >  2:57885/1‑247 (MQ=255)                                                                                                                                                                                                                             aCCAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATtcatc                       <  1:599262/249‑1 (MQ=255)                                                                                                                                                                                                                               cAGTGCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTa                   <  1:423099/251‑1 (MQ=255)                                                                                                                                                                                                                                   gCTTTAGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAg               <  2:472074/251‑1 (MQ=255)                                                                                                                                                                                                                                       tAGCCGGAAGATCGAATGCGTTAGCGTAGTGATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTCGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATGAAGCACGGAGTCATTCATCTCTACCAGCACg           >  2:661382/1‑251 (MQ=255)                                                                                                                                                                                                                                        aGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCAc                                                 <  2:185076/212‑1 (MQ=255)                                                                                                                                                                                                                                        aGCCGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCAc                                                 >  1:185076/1‑212 (MQ=255)                                                                                                                                                                                                                                           cGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGtt                                                                                                                              >  2:350150/1‑132 (MQ=255)                                                                                                                                                                                                                                           cGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGtt                                                                                                                              <  1:350150/132‑1 (MQ=255)                                                                                                                                                                                                                                           cGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCAc       <  1:233068/251‑1 (MQ=255)                                                                                                                                                                                                                                           cGGAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGATCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGCGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGCGTCATTCCTCACTACCAGCACGCCAc       >  2:480752/1‑251 (MQ=255)                                                                                                                                                                                                                                             gAAGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCGTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACgg     <  1:646472/251‑1 (MQ=255)                                                                                                                                                                                                                                               aGATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGtt   <  1:549096/251‑1 (MQ=255)                                                                                                                                                                                                                                                gATCGAATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGGGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACggctg  >  2:611056/1‑248 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CAGAACGCGCGCTTTTTGCGGGTTCAGCGTGCCAGAGGCGACGAAGCCGTATTTCGCATCATCCACTTCGGCATCCTGAGTGGTAGCGCCCGTCGGTACGCGGGAAGAACGCACGACTGCAGTACCGGTTTTCGCGGCGGTCGCCAGCGTGTCGAACACAGATTTATACAGGTTGCCGTTACCCACACCAGCGCTAACGATGCCATCATAGCCCGCATCTACCAGTGCTTTAGCCGGAAGATCGGATGCGTTAGCGTAGTTATAAACAATGCCGACTTTCGGCAGTTCATTCAGCTTAGAGACATCGAATGGCGTGTCGCTGGTATGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAGACTTGAAGGTCGCTACGTCGGTGGTGTTGGTTTTGGTGACGTCACGGCCATCAAGCACGGTGTCATTCATCACTACCAGCACGCCACGGTTG  >  NC_000913/3099748‑3100237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑