Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,417,140	C→T	P63P (CCC→CCT)	yjfP →	carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,417,140	0	C	T	100.0%	230.0 / NA	66	P63P (CCC→CCT)	yjfP	carboxylesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/31);  total (35/31)

AGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATCGGGCTATTTTACA  >  NC_000913/4416895‑4417383                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    agcagAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCa                                                                                                                                                                                                                                                <  1:49413/251‑1 (MQ=255)        aCTTGTGCTAAGGCTTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCAGATATTACCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGc                                                                                                                                                                                                                                         >  1:188840/1‑251 (MQ=255)        aCTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGc                                                                                                                                                                                                                                         <  1:642356/251‑1 (MQ=255)              gCTAAGGATTTCTTCATGTCATACCAGACAAAAATGATAGACAGATGATTGTAATAGAATCACGCGAGCTGGCAGATATGCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATATCTATTTTGCCGTTGCGCTGGCGCATGCTGTTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGtttt                                                                                                                                                                                                                                   <  1:83881/251‑1 (MQ=255)                tAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGtttt                                                                                                                                                                                                                                   >  1:447255/1‑249 (MQ=255)                 aaGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTa                                                                                                                                                                                                                                  <  1:294668/249‑1 (MQ=255)                    gATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAgtggt                                                                                                                                                                                                                             <  1:617113/251‑1 (MQ=255)                         cttcATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGc                                                                                                                                                                                                                        >  1:23289/1‑251 (MQ=255)                                         aCAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCGGGCAGATATTCCCGTTCTTCATGCGTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCGGGTGTATAGCTATTTTGCCGTTGCGCGGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCTCGGTAGCCGGGTTAGTGGTGACGCAGCGCGGCGGTTAAAt                                                                                                                                                                                                        <  1:30820/251‑1 (MQ=255)                                           aaaaaGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCa                                                                                                                                                                                                      <  1:103150/251‑1 (MQ=255)                                            aaaaGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCa                                                                                                                                                                                                      >  1:364774/1‑250 (MQ=255)                                               aGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTc                                                                                                                                                                                                  <  2:123854/251‑1 (MQ=255)                                                          atgatTGAAATAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCtt                                                                                                                                                                                       >  1:207927/1‑251 (MQ=255)                                                                 aaaTAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCCAAACTTGCTACa                                                                                                                                                                                 >  1:66178/1‑250 (MQ=255)                                                                  aaTAGAATCACGCGAGCTGTCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACaaa                                                                                                                                                                               <  1:246985/251‑1 (MQ=255)                                                                  aaTAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACaaa                                                                                                                                                                               <  2:647271/251‑1 (MQ=255)                                                                     aGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGt                                                                                                                                                                             >  1:407508/1‑250 (MQ=255)                                                                        aTCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATgg                                                                                                                                                                         >  2:500557/1‑250 (MQ=255)                                                                            cgcgAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAgg                                                                                                                                                                      >  1:129224/1‑250 (MQ=255)                                                                              cgAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAAt                                                                                                                                                                   <  2:70965/251‑1 (MQ=255)                                                                               gAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCCAATCTTGCTTCAAAGTATGCAGGAAtt                                                                                                                                                                  >  1:357640/1‑251 (MQ=255)                                                                               gAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAAtt                                                                                                                                                                  <  1:361743/251‑1 (MQ=255)                                                                               gAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAAtt                                                                                                                                                                  <  2:332186/251‑1 (MQ=255)                                                                                    ggCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTACCTCGGCTTTACTTCATCCATTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGAGCATGCCGGATGCGCCTGAGCACGGTAGCCGTTTTAGTGGGGACGCAGCGCGGCGGTTAAATCAATGCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCacg                                                                                                                                                              <  2:549763/250‑2 (MQ=255)                                                                                                             tATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCga                                                                                                                                       >  1:221821/1‑248 (MQ=255)                                                                                                                ccTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGAGCCTGATCACGGTAGCCGTTTTAGTGGGGACGCAGCGCGGCGGGTAAATCAATTTTGGCAAATCTTGCTACAAAGGATGCAGGGATTTACTACTTTAAGTGCGGCAATAGCCGAAGaaa                                                                                                                                  >  1:555223/1‑250 (MQ=255)                                                                                                                         cAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCa                                                                                                                                                                                             <  2:156640/182‑1 (MQ=255)                                                                                                                         cAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCa                                                                                                                                                                                             >  1:156640/1‑182 (MQ=255)                                                                                                                           aaaaGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGGATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTtg                                                                                                                      >  1:601714/1‑251 (MQ=255)                                                                                                                                aTACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAg                                                                                                                                                                                                                      >  1:463593/1‑150 (MQ=255)                                                                                                                                aTACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAg                                                                                                                                                                                                                      <  2:463593/150‑1 (MQ=255)                                                                                                                                     ccGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAATATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCt                                                                                                            >  1:416629/1‑251 (MQ=255)                                                                                                                                      cGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCCATTATGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTTCGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTg                                                                                                           >  2:189905/1‑251 (MQ=255)                                                                                                                                       gTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAAATGGCTGGTTGATGACCGTCTgg                                                                                                          >  2:187720/1‑251 (MQ=255)                                                                                                                                         tACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATACATTCTGGCAAATCTTGCTACAAAGAATGCAGGAATTTAATACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTngg                                                                                                         >  2:603187/1‑249 (MQ=255)                                                                                                                                           ccGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGt                                                                                                      >  2:469201/1‑251 (MQ=255)                                                                                                                                             gTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTCGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCCATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCg                                                                                                    >  2:479359/1‑251 (MQ=255)                                                                                                                                                 gTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTgg                                                                                                <  1:537865/251‑1 (MQ=255)                                                                                                                                                   aaTTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGgc                                                                                               <  2:348072/250‑1 (MQ=255)                                                                                                                                                           aTCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGAt                                                                                       <  2:364774/250‑1 (MQ=255)                                                                                                                                                                        ttCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACgg                                                                         >  1:108349/1‑251 (MQ=255)                                                                                                                                                                           aTCCAGTCGGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTTGTTTGCGGGTGATCATGCCGGATGCGCCTGATCGCGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTTGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGTCAc                                                                      <  2:188840/251‑1 (MQ=255)                                                                                                                                                                            tCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTTCTACAAAATATTCAg                                                                                                                                                                       >  1:430069/1‑153 (MQ=255)                                                                                                                                                                            tCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAg                                                                                                                                                                       <  2:430069/153‑1 (MQ=255)                                                                                                                                                                            tCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGGATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAAATGGCTGCTTGATGACCGTCTTGCAGTCGGTGGCGCGTCGATGGGCGCGGTGACGGCAcc                                                                     >  2:251589/1‑250 (MQ=255)                                                                                                                                                                             ccAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACt                                                                     <  2:221821/250‑1 (MQ=255)                                                                                                                                                                               aGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAACTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTTGGTGGCGCGTCGATGGGCGCGATGACGGCACTggg                                                                  >  1:423257/1‑251 (MQ=255)                                                                                                                                                                               aGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGGTAAATCAATTCTGGCAAATATTGCTACAAAGAATGCAGGAATTTAATACTTTACGTGGGGCAATAGGCGAAGAAAAATGGCTGATTGATGAACGTATGGCAGTCGGTGGGGCGTCGAGGGGCGCGCTGACGGCACTggg                                                                  >  1:582285/1‑251 (MQ=255)                                                                                                                                                                                       gtATAGCTATTTTGCAGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGGATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGGAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTAccc                                                          >  1:483909/1‑250 (MQ=255)                                                                                                                                                                                            gCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAAACAATTCTGGCAAATATTGCTAACAAGGATTAAGGAATTCACTACCTTACGTGAGGCAATATCCGAAGAAAACTGGCTGCTTGTTGAACGTCTTGCAGTCGGTGGCGCGTCGATGGGGGGGATGATGGcgcctggggttaccccccgc                                                     >  2:616839/1‑232 (MQ=255)                                                                                                                                                                                                  ttGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATgg                                                                                     <  1:367203/213‑1 (MQ=255)                                                                                                                                                                                                    gCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCAc                                             >  1:459468/1‑251 (MQ=255)                                                                                                                                                                                                       gTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCCATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCCCTGGGGGTTACCGCTCGCCACCCCCCggg                                          >  1:100307/1‑250 (MQ=255)                                                                                                                                                                                                           cgcTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGGCCTGGGGATTACCGCTCGCCACCCCACGGTgagg                                      >  2:183309/1‑250 (MQ=255)                                                                                                                                                                                                            gcTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGGGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTg                                         >  1:475867/1‑247 (MQ=255)                                                                                                                                                                                                              tGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGAGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTTCTACAAAGTATGCAGGAATTCACTACTTTACGGGCGGCAATAGCCTAAGAAAACTGGCTGCTTGATTACCGTCTGGTAGTCTGTGGCGCGGCGAGGGGCGCGATGACGGCACTGGGGATTAACGATCGCCAACCCACTGTGAGgtgt                                   >  1:202653/1‑251 (MQ=255)                                                                                                                                                                                                                  gcAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGATGTACCg                               <  1:124952/251‑1 (MQ=255)                                                                                                                                                                                                                    aGGNTGGTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGATGTACCGcc                             <  2:601714/251‑1 (MQ=255)                                                                                                                                                                                                                         ggTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCCCTGGGGATTACCGCTCGCCACCCCACGGTGAGATGTACCGCCAGCat                        >  1:114690/1‑251 (MQ=255)                                                                                                                                                                                                                         ggTTTGCGGGTGATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGATGTACCGCCAGCat                        <  1:603187/251‑1 (MQ=255)                                                                                                                                                                                                                            ttGCGGGTGGTCGTGCCGGATGCGCTTGATCCCGGTAGCCGTTTTAGGGGGGAAGCAGCGCGGCGGTTAAATAAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTATTGGCCGTCTGCCAGTCGGTGGGGGTTCGATGGGCGCGATGACGGCACTGGGGATTACCTCTCGCCTCCCCACGGTGAGATGTACCGCCAGCatga                      <  2:207927/250‑1 (MQ=255)                                                                                                                                                                                                                                    gATCATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGAGGGGCGCGATGACGGCACTGGGGATCACCGCTCGCCCCCCCACGGGGAGATGAACCGCCAGCATGATGGGATCgg             <  1:187720/251‑1 (MQ=255)                                                                                                                                                                                                                                       cATGCCGGATGCGCCTGATCCCGGTAGCCGTTTTAGTGGTGACGCAGCGCTGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATCGGGCt          <  1:528760/251‑1 (MQ=255)                                                                                                                                                                                                                                       cATGCCGGATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTGCCGCTCGCCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATCGGGCt          <  2:407508/251‑1 (MQ=255)                                                                                                                                                                                                                                              gATGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAAACAATTCTGGCAAATCTTTCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAACCGAAGAAAACTGGCTGCTTTATGACCGTCTGGCAGTCCGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGGGAGATGTACCGCCAGCATGATGGGATCGGGCTATTTTa    >  1:262045/1‑250 (MQ=255)                                                                                                                                                                                                                                               aTGCGCCTGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATCGGGCTATTTTACa  <  1:506141/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    AGCAGAAACTTGTGCTAAGGATTTCTTCATGTCATACCAGACAAAAAGGAGAGACAGATGATTGAAATAGAATCACGCGAGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATCGGGCTATTTTACA  >  NC_000913/4416895‑4417383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑