Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,962,183	C→T	A71V (GCT→GTT)	yecT →	protein YecT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,962,183	0	C	T	100.0%	104.4 / NA	33	A71V (GCT→GTT)	yecT	protein YecT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/15);  total (18/15)

GTTGAATTTAAGGAATACCGCAGTGTTTAAATTTCTTGTATTAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCAGCTATACCGTAACTCTGAA  >  NC_000913/1961950‑1962427                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     gTTGAATTTAAGGAATACCGCAGTGTTTAAATTTCTTGTATTAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAAc                                                                                                                                                                                                                                     >  1:514949/1‑251 (MQ=255)         tAAGGAATACCGCAGTGTTTAAATTTCTTGTATTAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGcc                                                                                                                                                                                                                              <  1:72010/250‑1 (MQ=255)               aTACCGCAGTGTTTAAATTTCTTGTATTAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGcac                                                                                                                                                                                                                        >  1:509350/1‑250 (MQ=255)                                   cTTGTATTAAAATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATAc                                                                                                                                                                                                   >  2:300467/1‑251 (MQ=255)                                       tATTAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGa                                                                                                                                                                                                <  2:564530/250‑1 (MQ=255)                                        aTTAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGaac                                                                                                                                                                                              >  2:12749/1‑251 (MQ=255)                                          tAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGaaca                                                                                                                                                                                             <  2:458385/250‑1 (MQ=255)                                           aaCATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGaacaa                                                                                                                                                                                            <  1:251040/250‑1 (MQ=255)                                                 aGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGCCCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACaacttaact                                                                                                                                                                                     >  1:401034/1‑251 (MQ=255)                                                  ggCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACaacttaactt                                                                                                                                                                                    <  2:420922/251‑1 (MQ=255)                                                    cATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTACCTTGc                                                                                                                                                                                  <  2:126908/251‑1 (MQ=255)                                                        ctctcTTGCCAGGCATACGCAGAGGATACGGTTATAGTCAACGCCCATGACATTTCAGACATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGACTACAATCTCGTCGATGCGCAATTAAATCAAGCCTATGGTGAAGTTTATCGTTATATAGAACAACTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTCACTTGCTTa                                                                                                                                                                               >  2:383707/2‑250 (MQ=255)                                                        aTCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCAACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGATTa                                                                                                                                                                               >  2:309920/1‑250 (MQ=255)                                                                                             tAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGANaa                                                                                                                                          >  2:598701/1‑250 (MQ=255)                                                                                                 cGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATAAGGACGTTAACGTGATAAAATCATGACTGCGACAAGAATACAATCTCGTCGATGAGCAATTAAATAAAGCCTATGGGGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAAATTAACATGCGTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAAt                                                                                                                                     <  2:302965/251‑1 (MQ=255)                                                                                                        gACAGTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTGAACTTGCTGAAAAAAACACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAAt                                                                                                                               <  2:539278/250‑1 (MQ=255)                                                                                                         aCATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAAtt                                                                                                                              >  1:594545/1‑250 (MQ=255)                                                                                                           aTTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAAt                                                                                                                           >  2:274951/1‑251 (MQ=255)                                                                                                                      cAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAAt                                                                                                                 <  1:435557/250‑1 (MQ=255)                                                                                                                               ttgGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTc                                                                                                       >  2:57376/1‑251 (MQ=255)                                                                                                                                      aaaaaTTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCATAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGAttt                                                                                                <  2:509350/251‑1 (MQ=255)                                                                                                                                       aaaaTTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGAttt                                                                                                >  1:171434/1‑250 (MQ=255)                                                                                                                                                           gACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATc                                                                           <  1:119323/251‑1 (MQ=255)                                                                                                                                                            acgttaacgttaTCACATCATGCCTGCGACAAGACTACAATCTCGTCTATGCGCAATTCAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACCGGTGTAACAAAACCTGATACCGAACAACTTAACTTGCTTAAAATATCACAGCGCGCCTGGCTGCATTTTAGGGACAAAGACTTTGAATTAATACTTTCAAATGTGGACGTTCAGGATTTAATAGACCCTTATTCTGAATc                                                                           >  2:516870/1‑250 (MQ=255)                                                                                                                                                             cGTTAACGTGTTCACATCATGCCTGAGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAANc                                                                           >  2:594276/1‑249 (MQ=255)                                                                                                                                                                            aTCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTa                                                          <  2:638615/251‑1 (MQ=255)                                                                                                                                                                              cATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTAt                                                         <  2:468438/250‑1 (MQ=255)                                                                                                                                                                                           agaatgaaaagtcaacgccgccaaaTTAAATAAAGCCTCTGGTGAAGTTTCTCGTAATATAGAACAAGTGCCACGAACAGGCGTAAAAAAACCTGATACAGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTCCAGGATTCAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGAccc                                           >  2:264294/24‑250 (MQ=255)                                                                                                                                                                                            gAATACAATCTCGTCGATGCGCAATTACATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCa                                           >  1:477585/1‑250 (MQ=255)                                                                                                                                                                                            gAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCaa                                          <  1:128437/251‑1 (MQ=255)                                                                                                                                                                                             aaTACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAAt                                         >  1:625143/1‑251 (MQ=255)                                                                                                                                                                                                                   aTTAAATAAAGCCTATGGTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCAAAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCAGc                   >  1:57490/1‑251 (MQ=255)                                                                                                                                                                                                                                    gTGAAGTTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACATCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCATCTATACCGTAACTCTGaa  <  1:251116/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     GTTGAATTTAAGGAATACCGCAGTGTTTAAATTTCTTGTATTAACATTAGGCATTATCTCTTGCCAGGCTTACGCAGAAGATACGGTTATAGTAAACGACCATGACATTTCAGCCATCAAAGATTGTTGGCAAAAAAATTCAGATGATGATACTGACGTTAACGTGATCAAATCATGCCTGCGACAAGAATACAATCTCGTCGATGCGCAATTAAATAAAGCCTATGGTGAAGCTTATCGTTATATAGAACAAGTGCCACGCACAGGTGTAAAAAAACCTGATACCGAACAACTTAACTTGCTTAAAAAATCACAGCGAGCCTGGCTGGATTTTAGGGACAAAGAATGTGAATTAATCCTTTCAAATGAGGACGTTCAGGATTTAAGTGACCCTTATTCTGAATCAGAATGGCTCTCATGTATGATCATACAGACCAATACGCGAACTCGCCAGTTGCAGCTATACCGTAACTCTGAA  >  NC_000913/1961950‑1962427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑