Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,397,434	C→T	P8L (CCA→CTA)	mutL →	DNA mismatch repair protein MutL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,397,434	0	C	T	100.0%	312.2 / NA	93	P8L (CCA→CTA)	mutL	DNA mismatch repair protein MutL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (54/39);  total (54/39)

TATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATT  >  NC_000913/4397189‑4397678                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     tatCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAAc                                                                                                                                                                                                                                                 >  2:129073/1‑251 (MQ=255)     ccATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGc                                                                                                                                                                                                                                             >  1:651753/1‑251 (MQ=255)            tACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAAcc                                                                                                                                                                                                                                        >  2:166004/1‑249 (MQ=255)               tGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGAtt                                                                                                                                                                                                                                   >  1:169976/1‑251 (MQ=255)                    gAAACCGGTTTTATCAGCAACAACAGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGc                                                                                                                                                                                                                              <  1:438652/251‑1 (MQ=255)                       aCCGGTTTTATCACGAACAACAGCGAAGAACGTTTGCTGGCGCGCGTCGATTACCATCAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTGCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAACCTGCCAGTACGGTGACGACGTCAGATCGCCCGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTGCAACTGGTGAACCAGATTGCCGCagg                                                                                                                                                                                                                           <  2:482546/251‑1 (MQ=255)                          ggTTTTATCAGCAACAACAGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCaggtgg                                                                                                                                                                                                                        >  2:257340/1‑250 (MQ=255)                          ggTTTTATCAGCAACAACAGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCaggtg                                                                                                                                                                                                                         >  2:21560/1‑250 (MQ=255)                                aTCAGCAACAACAGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAggtggg                                                                                                                                                                                                                  >  2:174148/1‑250 (MQ=255)                                aTCAGCAACAACAGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATGGCCGCAGGTGAggtggt                                                                                                                                                                                                                  <  1:199699/251‑1 (MQ=255)                                   aGCAACAACAGCGAAGTACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCACTTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGCTCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGACGCAGGTGAGGTGGTCg                                                                                                                                                                                                                >  2:352642/1‑250 (MQ=255)                                      aacaacAGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCg                                                                                                                                                                                                            >  1:149176/1‑251 (MQ=255)                                        caacaGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGat                                                                                                                                                                                                          <  1:29683/251‑2 (MQ=255)                                            aGCGAAGGACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGCCGAGCGATCTGc                                                                                                                                                                                                      >  1:142407/1‑251 (MQ=255)                                            aGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGc                                                                                                                                                                                                      >  2:273270/1‑251 (MQ=255)                                               gaagaaCGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGGCGAGCGATCTGCGTc                                                                                                                                                                                                   >  2:2648/1‑251 (MQ=255)                                                aagaaCGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCg                                                                                                                                                                                                  <  1:33034/251‑1 (MQ=255)                                                   aaCGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTa                                                                                                                                                                                               <  2:34647/251‑1 (MQ=255)                                                   aaCGTTTGCTGGCGAGAGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGt                                                                                                                                                                                                >  2:390647/1‑250 (MQ=255)                                                    aCGTTTGCTGGCGAGCGCCGATTACCAACAACAGCTGGCAGACGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGt                                                                                                                                                                                                >  1:519259/1‑249 (MQ=255)                                                        ttGCTGGCGATCGACGATTACCAACAACAGCTGGCAGAAGCCAATTACAAAGGCCTGCGCAATTACTTCCTTTCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTGCCGCTACATCTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGTTAGTCa                                                                                                                                                                                           <  2:271881/250‑1 (MQ=255)                                                                gagcgaCGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACt                                                                                                                                                                                    >  1:487432/1‑249 (MQ=255)                                                                  gcgaCGAGTACCAACAACAGCGGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACGTCCTTGCGCATCCGATGCAATCTTCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTg                                                                                                                                                                                <  2:145947/251‑1 (MQ=255)                                                                    gacgaTTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGTTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGa                                                                                                                                                                                                                                           <  2:427742/190‑1 (MQ=255)                                                                    gacgaTTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGa                                                                                                                                                                                                                                           >  1:427742/1‑190 (MQ=255)                                                                     acgaTTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGa                                                                                                                                                                              >  2:22159/1‑250 (MQ=255)                                                                       gaTTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGaaa                                                                                                                                                                            >  1:357709/1‑250 (MQ=255)                                                                            ccAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGCCGGTAGTCAAAGAACTAGTGGAAAACAGcc                                                                                                                                                                      >  1:382031/1‑251 (MQ=255)                                                                             caacaaCAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCt                                                                                                                                                                     >  1:210494/1‑251 (MQ=255)                                                                              aacaacAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTc                                                                                                                                                                    >  1:91703/1‑251 (MQ=255)                                                                              aacaacAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCAGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGACAACAGCCTc                                                                                                                                                                    >  2:366789/1‑251 (MQ=255)                                                                               acaacaGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCg                                                                                                                                                                   >  1:273143/1‑251 (MQ=255)                                                                                        ggCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTg                                                                                                                                                          <  2:535133/251‑1 (MQ=255)                                                                                          cAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGCGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTAGGTAGTCAAAGCACTAGTGGAAAACAGCCTCGATGCAGGTGCg                                                                                                                                                        >  1:151888/1‑251 (MQ=255)                                                                                              aGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACGGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGAcgc                                                                                                                                                    <  1:375471/251‑1 (MQ=255)                                                                                               gCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGGGCGAcgcg                                                                                                                                                   >  1:166882/1‑251 (MQ=255)                                                                                               cccATTTACAAAGGCCTGCGCAATTACTTCCTTGCCCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCTAcgcg                                                                                                                                                   <  2:142407/250‑1 (MQ=255)                                                                                                 cATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCCGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCATAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGt                                                                                                                                                  >  2:611724/1‑250 (MQ=255)                                                                                                        aaaGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGatat                                                                                                                                          >  1:626535/1‑251 (MQ=255)                                                                                                        aaaGGCCTGCGCAAGTACTTCCTGGCGCATCCGATGCAAGCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGGGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGatat                                                                                                                                          <  2:146012/251‑1 (MQ=255)                                                                                                         aaGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATAtt                                                                                                                                         <  2:501815/251‑1 (MQ=255)                                                                                                          aGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTg                                                                                                                                        <  1:137316/251‑1 (MQ=255)                                                                                                                gcgcAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGGTCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGatat                                                                                                                                    <  2:221561/249‑1 (MQ=255)                                                                                                                   cAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAAc                                                                                                                               >  1:126656/1‑251 (MQ=255)                                                                                                                    aaTTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAAcg                                                                                                                              <  1:395618/251‑1 (MQ=255)                                                                                                                       tACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCgg                                                                                                                           >  2:351775/1‑251 (MQ=255)                                                                                                                        aCTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGt                                                                                                                          <  1:283233/251‑1 (MQ=255)                                                                                                                           tCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAATCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTggg                                                                                                                       >  2:622322/1‑251 (MQ=255)                                                                                                                           tCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTggg                                                                                                                       >  1:51335/1‑251 (MQ=255)                                                                                                                           tCCTTGCGCATCCGATACAAGCTGCGCCTCCGGCTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTTCCAAACTAAGGACGATTGATGCCAATTCAGTTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTg                                                                                                                         <  1:496934/249‑1 (MQ=255)                                                                                                                           tCCTTGCCCATCCTATGCATGCTGCTCCGCAGGTTTCAAGGCCACAAACTGCCTGTGCGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATGTGCGTCGGTAGTCAAAGAACTATTGGAAACCAGCCTCGCTGCAGGTGCGACGCGTTTCGATAGTGATAGCGGACGCGGtt                                                                                                                         <  2:369329/249‑2 (MQ=255)                                                                                                                                gcgcATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGa                                                                                                                   <  1:360203/250‑1 (MQ=255)                                                                                                                                    aTCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGAGACGAACCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTAAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCCGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGGGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACtt                                                                                                              >  1:603238/1‑251 (MQ=255)                                                                                                                                        gATGCTATCTTCGCCGCAGGGTGCAACGGCACACACCGCCCGTACGGTGACGACGCCAGATCGCACGCTGCCCAACTAAGGACGATTGATGCCAATTCAGGTCTTACCACTACATCCGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGATCGATCTGCGTCGGTAGTCAAAGAACGCGTGGAAAACAGCCTCGATTCAGGTGCGACGCGTATCGATATTGATTTCGAACGCGGTGGGGCGAAATTTATcc                                                                                                          <  1:166962/251‑1 (MQ=255)                                                                                                                                        gATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGGCGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGACTTACCGCTACAACTGGCGACCCAGATTGCCGCAGGGGAGGTGGTCGAGCGATCTGCGTCCGTAGTCAAAGAAATAGTGGAAAACAGCCTCGATGGAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATcc                                                                                                          >  1:586631/1‑251 (MQ=255)                                                                                                                                         aTGCAATCTGCTCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGGCGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCg                                                                                                         <  2:90473/251‑1 (MQ=255)                                                                                                                                           gCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCa                                                                                                       <  1:622322/251‑1 (MQ=255)                                                                                                                                             aaTCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCAtt                                                                                                     >  2:566195/1‑251 (MQ=255)                                                                                                                                              accTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTc                                                                                                    <  2:114152/249‑1 (MQ=255)                                                                                                                                                  gcgcCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTc                                                                                                    <  2:463932/247‑1 (MQ=255)                                                                                                                                                         aGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                                                         <  1:257340/251‑1 (MQ=255)                                                                                                                                                         aGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGGGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                                                         >  2:471767/1‑251 (MQ=255)                                                                                                                                                               cAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGt                                                                                   <  2:224763/251‑1 (MQ=255)                                                                                                                                                                  cGGCACAAACTGCCAGGCCGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGGGTGATGCCAATTCAGGGTTTACCGCTACAACTGGCGAACCAGAGTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACATCCTCGATGCATGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATc                                                                                <  2:556340/251‑1 (MQ=255)                                                                                                                                                                   ggCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCACTTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGACGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATACGCATTCGTGATAACGGCTGCGGTATc                                                                                >  1:167420/1‑250 (MQ=255)                                                                                                                                                                      aaaaaCTGCCAGTACGGGAACGAGGCCAGAGCGCACGCTGCCAAACTAAGGACGAGTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaa                                                                            <  2:205361/249‑1 (MQ=255)                                                                                                                                                                        aaaCTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaa                                                                           >  2:425616/1‑250 (MQ=255)                                                                                                                                                                            tGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATg                                                                      >  1:494764/1‑251 (MQ=255)                                                                                                                                                                             gCCAGTACGGTGACGACACCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGGGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCGCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCGGCGGTATCAAAAAAGATg                                                                      >  2:576584/1‑250 (MQ=255)                                                                                                                                                                              ccAGTACGGTGACGACGCCAGATCGCACGCTGCCACACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATg                                                                      >  2:380795/1‑249 (MQ=255)                                                                                                                                                                                aGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGatat                                                                                                                                          <  2:195974/179‑1 (MQ=255)                                                                                                                                                                                aGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGatat                                                                                                                                          >  1:195974/1‑179 (MQ=255)                                                                                                                                                                                   aCGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggc                                                               >  1:84923/1‑251 (MQ=255)                                                                                                                                                                                            gcgCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgct                                                      <  2:487432/250‑1 (MQ=255)                                                                                                                                                                                             cgCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                                                     >  2:129038/1‑251 (MQ=255)                                                                                                                                                                                                      gCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCAt                                            <  1:503134/251‑1 (MQ=255)                                                                                                                                                                                                         cGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGcc                                         >  1:620031/1‑251 (MQ=255)                                                                                                                                                                                                              ccAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc                                      >  2:397378/1‑249 (MQ=255)                                                                                                                                                                                                                  aCTAAGGACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaa                                 >  2:406890/1‑250 (MQ=255)                                                                                                                                                                                                                     aaGGACGATTGCTGCCAATTCAGAGCTGACCGCTACAACTGGCGACCCATATTGCCGCAGGTTAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTTGCGCTGGCTCGTCATGCCCCCAGTAAAATc                             <  2:451739/251‑1 (MQ=255)                                                                                                                                                                                                                       ggACGATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGc                           <  1:21560/251‑1 (MQ=255)                                                                                                                                                                                                                           gATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATGGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTGTCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCctct                       >  1:13466/1‑251 (MQ=255)                                                                                                                                                                                                                           gATTGATGCCAATTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGGGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCctct                       <  2:84923/251‑1 (MQ=255)                                                                                                                                                                                                                                      aTTCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGAtct            >  2:36126/1‑251 (MQ=255)                                                                                                                                                                                                                                        tCAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCg          >  1:582922/1‑251 (MQ=255)                                                                                                                                                                                                                                         cAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCg          <  1:66085/250‑1 (MQ=255)                                                                                                                                                                                                                                         cAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCg          >  1:182329/1‑250 (MQ=255)                                                                                                                                                                                                                                         cAGGTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCg          <  1:107586/250‑1 (MQ=255)                                                                                                                                                                                                                                           ggTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAg       <  1:380795/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGAGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGa         >  1:100556/1‑249 (MQ=255)                                                                                                                                                                                                                                            gTCTTACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGc      >  1:46204/1‑251 (MQ=255)                                                                                                                                                                                                                                               ttACCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCAt   >  1:463082/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aCCGCTACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGATCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCAtt  >  1:465807/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     TATCCCATCAGTACTGGTCGAAACCGGTTTTATCAGCAACAACAGCGAAGAACGTTTGCTGGCGAGCGACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGCGCCGCAGGGTGCAACGGCACAAACTGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAAGGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCGCCTCTCTGGACGATCTCGAAGCCATT  >  NC_000913/4397189‑4397678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑