Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,191,118 C→T L163L (CTA→TTA)  yqiI → putative fimbrial protein YqiI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,191,1180CT96.0% 67.6 / ‑6.0 25L163L (CTA→TTA) yqiIputative fimbrial protein YqiI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (5/19);  minor base G (1/0);  total (6/19)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAGCACCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAGCACTTGAATTTAAAGGTATGAGCGGAGCAGGTGCTATAGAAGTAGATAAAAATCTGACCTTCAGGATTCGCGGATTAAATAACATTCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATA  >  NC_000913/3190875‑3191364
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      
gCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAGCACCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTtatat                                                                                                                                                                                                                                                   <  1:602016/249‑1 (MQ=255)
gCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAGCACCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTtat                                                                                                                                                                                                                                                     <  2:175561/247‑1 (MQ=255)
                 gatgTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAGCACCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAAt                                                                                                                                                                                                                                 <  1:298332/250‑1 (MQ=255)
                    gttgttAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAGCACCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCa                                                                                                                                                                                                                             <  1:41085/251‑1 (MQ=255)
                       gttAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAGCACCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCAAt                                                                                                                                                                                                                           <  1:42636/250‑1 (MQ=255)
                          aGCATCAAGTCAAGACTTTTATAATCAAAACTCTTATACTTGCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTTCCTATTGCAGCACCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACAAGTGCAGTTATATATCAAGCTAGCTAAAAATGCCAATaaa                                                                                                                                                                                                                        <  2:378372/250‑1 (MQ=255)
                            cATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAGCACCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGta                                                                                                                                                                                                                     <  2:313230/251‑1 (MQ=255)
                                  gTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAGCACCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGtaaataaa                                                                                                                                                                                                               <  1:269271/251‑1 (MQ=255)
                                                            tataCTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAGCACCTGTATGTATGGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGTCGAatat                                                                                                                                                                                       <  1:456165/249‑1 (MQ=255)
                                                                cgtgcGGTGGAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAGCACCTGTATGGATTGATATAAAGGGAGCCGGAACCGTCTGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTTCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAATCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTGCCTGAc                                                                                                                                                                                             <  1:532717/235‑1 (MQ=255)
                                                                               ataATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAGCACCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCCGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCATTTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAGCACTTGAATTTAAAGGt                                                                                                                                                                  <  1:653012/251‑1 (MQ=255)
                                                                                 aaTGGGGTTGATTATGATTCTACATCTCCCCTACCTATTGCAGCACCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAGCACTTGAATTTAAAGGTa                                                                                                                                                                 <  2:429012/250‑1 (MQ=255)
                                                                                                                           gcaCCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATTAGGAACTTTTTCCGTACGAGGGGAGTTATATATTTAACTAGCTAAAAATGCCCATAAAGTAAATAAAAAAATTGTATTTCATTACGAATATATAACACTTTAATTTAAAGGTATGAGCGGAGGCGGGGCTATAAAAGTAGATAAAAATCTTACCtt                                                                                                                      >  1:34301/1‑251 (MQ=255)
                                                                                                                            caCCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTTAAACGCAATTTACAGTAAATTTTTCCGTAAGAGTGCAGGTTTATATTAAGCTAGCGAAAAATGCCAATAAAGAAAATAAAAAAATTTTATTTCCCGACGAATATATAGCACTTGTATTTAAAAGGATGAGCGGAGCAGGGGCTATAGAAGTAGATAAAAAACTTACCTTc                                                                                                                     >  1:425551/1‑251 (MQ=255)
                                                                                                                                 gtatgtatTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGG‑GGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAACCTTGTATTACCTGACGAATATATAGCACTTGAATTTAAAGGTATGAGCGGAGCAGGTGCTATAGAAGTAGATAAAAATCTGACCTTCAGGAtt                                                                                                               >  2:450875/1‑251 (MQ=255)
                                                                                                                                          gATATAAATGGAGCCGGAACCTTCGGGAATGGCTCCAAAAATCCCGCAGTCTGTACTGGCGGTTCTGTGCCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTCTATTACGTGACGAATATATAGCCCTTGAACTTAAAGGTATGAGCTGAGCAGGTGCTATAGAAGTAGATAAAAATCTGACCTTCAGGATTcgtagatt                                                                                                       <  2:231705/251‑7 (MQ=255)
                                                                                                                                                       ccGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAGCACTTGAATTTAAAGGTATGAGCGGAGCAGTTGCTATAGAAGTAGATAAAAATCTGACCTTCAGGATTCGCGGATTAAATAACATTCAt                                                                                          <  1:444334/251‑1 (MQ=255)
                                                                                                                                                                         gCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAGCACTTGAATTTAAAGGTATGAGCGGAGCAGGTGCTATAGAAGTAGATAAAAATCTGACCTTCAGGATTCGCGGATTAAATAACATTCATGTCCTTGACTGCTTTGtt                                                                        <  1:317938/251‑1 (MQ=255)
                                                                                                                                                                                                     cGGTCCTGAACCGCAATTATCAGTAACTTGTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAGCACTTGAAGTTAAAGGTATGAGTGTAGCATGTGCTATAGAAGTAGATAAAAATCTGACCTTCAGGATTCGCGGATTAAATAACATTCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCg                                            <  2:169153/251‑1 (MQ=255)
                                                                                                                                                                                                      ggTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAGCACTTGAATTTAAAGGTATGAGCGGAGCAGGTTCTATAGAAGTAGATAAAAATCTGACCTGCAGGATTCGCGGATTAAATAACATTCATTTCCTTGACTGCTGTGTTAATGTTGATCTGGAACCAGCTGATGGCGt                                           <  1:257954/251‑1 (MQ=255)
                                                                                                                                                                                                         ccTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAGCACTTGAATTTAAAGGTATGAGCGGAGCATGTGCTATAGAATTACATAACAATCTGACCTTCAGGATTCGCGGATTAAATAACATTCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGt                                        <  2:100956/251‑1 (MQ=255)
                                                                                                                                                                                                           tGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGTTATATATTGAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAGCACTTGAATTTAAAGGTATGAGCGGAGCAGGTGCTATAGAAGTAGATAAAAATCTGACCTTCAGGATTCGCGGATTAAATAACATTCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCg                                      >  1:576613/1‑251 (MQ=255)
                                                                                                                                                                                                                                   tCCAGTACGAGTGCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAGCACTTGAATTTAAAGGTATGAGCGGAGCAGGTGCTATAGAAGTAGATAAAAATCTGACCTTCAGGATTCGCGGATTAAATAACATTCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGa               >  2:657831/1‑250 (MQ=255)
                                                                                                                                                                                                                                               gCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAGCACTTGAATTTAAAGGTATGAGCGGAGCAGGTGCTATAGAAGTAGATAAAAATCTGACCTTCAGGATTCGCGGATTAAATAACATTCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAAt   >  1:384773/1‑250 (MQ=255)
                                                                                                                                                                                                                                               gCAGTTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATAGAGCACTTGAATTTAAAGGTATGAGCGGAGCAGGTGCTATAGAAGTAGATAAAAATCTGACCTTCAGGATTCGCGGATTAAATAACATTCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCCACTTTGGTAAAATAAATTCCCGAACAAGTAAAAATa  <  2:400256/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      
GCATGGGCAAATATAATGATGTTGTTAGCATCAGGTCAAGACTTTTATAATCAAAACTCTTATACTTTCGGTGTAACTTATAATGGGGTTGATTATGATTCTACATCTCCACTACCTATTGCAGCACCTGTATGTATTGATATAAAGGGAGCCGGAACCTTCGGGAATGGCTACAAAAAGCCCGCAGTCTGTAGTGGCGGTCCTGAACCGCAATTATCAGTAACTTTTCCAGTACGAGTGCAGCTATATATTAAGCTAGCTAAAAATGCCAATAAAGTAAATAAAAAACTTGTATTACCTGACGAATATATAGCACTTGAATTTAAAGGTATGAGCGGAGCAGGTGCTATAGAAGTAGATAAAAATCTGACCTTCAGGATTCGCGGATTAAATAACATTCATGTCCTTGACTGCTTTGTTAATGTTGATCTGGAACCAGCTGATGGCGTTGTCGACTTTGGTAAAATAAATTCCCGAACAATTAAAAATA  >  NC_000913/3190875‑3191364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑