Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,929,881	C→T	G405S (GGC→AGC)	ravA ←	regulatory ATPase RavA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,929,881	0	C	T	100.0%	214.4 / NA	62	G405S (GGC→AGC)	ravA	regulatory ATPase RavA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/35);  total (27/35)

AGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGAATTACCGTTAAGGCTGTTTTATCGCTTTGTTGCTGCTGGAGTTGCAGGT  >  NC_000913/3929640‑3930126                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      aGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGtt                                                                                                                                                                                                                                              >  1:659941/1‑251 (MQ=255)           cAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTAcc                                                                                                                                                                                                                                     <  1:254304/250‑1 (MQ=255)           cAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACcg                                                                                                                                                                                                                                    <  2:150028/251‑1 (MQ=255)                            tCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCttt                                                                                                                                                                                                                   >  1:239712/1‑251 (MQ=255)                               ccAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCt                                                                                                                                                                                                                >  2:393727/1‑251 (MQ=255)                                  gggATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTc                                                                                                                                                                                                               >  2:125722/1‑249 (MQ=255)                                      tacaCAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGTGAAGTGCCAGCGTACTGCCTTTTAACCTTACATCGCGACCGACAAGATGTTGGGCGCTATCAACTTCCAGATTCCGTTTCTGAGCATCGCTGATACCGTTCAGTTTACCGCGACTTTCACCACCTTTGCTCAGCCAc                                                                                                                                                                                                         >  2:332273/4‑251 (MQ=255)                                       aaaCAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACt                                                                                                                                                                                                        >  2:198696/1‑251 (MQ=255)                                        aaCAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTg                                                                                                                                                                                                       <  1:384933/251‑1 (MQ=255)                                              cATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAg                                                                                                                                                                                                 <  1:496532/251‑1 (MQ=255)                                               aTTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAgc                                                                                                                                                                                                <  1:614920/251‑1 (MQ=255)                                               aTTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGCGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAgc                                                                                                                                                                                                <  2:411898/251‑1 (MQ=255)                                                   ttGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTTTTGTTTTATTTCACCACGCAGACCTTCATCCGATGAACCGGTAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGACGTTCGGCGCTATCAACTTCCAGATTCAGTTTCTGTGCACAGCTGCTACCGCTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAgcgcg                                                                                                                                                                                             <  1:588298/250‑1 (MQ=255)                                                          gcGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTATTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTTCCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTc                                                                                                                                                                                     <  1:277224/250‑1 (MQ=255)                                                               gAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCaaa                                                                                                                                                                                  <  1:501664/249‑1 (MQ=255)                                                               gAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAgg                                                                                                                                                                                <  2:13185/251‑1 (MQ=255)                                                                aaTAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAgg                                                                                                                                                                                >  2:19809/1‑250 (MQ=255)                                                                      gCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATg                                                                                                                                                                         <  2:148312/251‑1 (MQ=255)                                                                       cGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGa                                                                                                                                                                        <  1:577741/251‑1 (MQ=255)                                                                       cGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGCTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATg                                                                                                                                                                         >  1:578677/1‑250 (MQ=255)                                                                          gtTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAAcc                                                                                                                                                                     <  2:106962/251‑1 (MQ=255)                                                                           ttgcttgcGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAAcc                                                                                                                                                                     >  1:584290/1‑250 (MQ=255)                                                                                   ggcattCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCATCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCata                                                                                                                                                            <  2:570858/246‑1 (MQ=255)                                                                                      aGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATc                                                                                                                                                          >  1:50322/1‑250 (MQ=255)                                                                                         cGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATg                                                                                                                                                       <  1:553902/250‑1 (MQ=255)                                                                                             ttCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAg                                                                                                                                                    <  1:21888/249‑1 (MQ=255)                                                                                               cAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGtttt                                                                                                                                                >  1:513090/1‑251 (MQ=255)                                                                                               cAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGttt                                                                                                                                                 <  2:659941/250‑1 (MQ=255)                                                                                                 aGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCa                                                                                                                                                                                                  >  2:239402/1‑199 (MQ=255)                                                                                                 aGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCa                                                                                                                                                                                                  <  1:239402/199‑1 (MQ=255)                                                                                                     ctTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACg                                                                                                                                            <  2:576050/249‑1 (MQ=255)                                                                                                       tCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGtt                                                                                                                                         <  1:474481/250‑1 (MQ=255)                                                                                                        ccAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGtttt                                                                                                                                       <  2:504868/251‑1 (MQ=255)                                                                                                         cAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACAGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTACCGGttttt                                                                                                                                      >  2:337902/1‑251 (MQ=255)                                                                                                              ggtgtgttATTTAACCAGGCAGAAATGCAGCCGATGAAAGGGGAAGTGGCAGGGTACGAGAGGGTAAACTTACAACGAGAACGATGAGATGTTGGGAGATATCAAATTAAAGATTCAGTTTCTGGGAAAAGCTGATACCGTTCAGATTAACACGAATTTCAACACTTTTGCACAGCCACTGGTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCAAATAAGGCAGTTTTAACGGTTCTTgcagg                                                                                                                                 <  2:145431/245‑2 (MQ=255)                                                                                                                                gCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATCCCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGaa                                                                                                               <  2:514358/251‑1 (MQ=255)                                                                                                                                          aGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGt                                                                                                     >  1:297881/1‑251 (MQ=255)                                                                                                                                                      gggAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCACCTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGtt                                                                                         <  2:517996/251‑1 (MQ=255)                                                                                                                                                          aGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGAt                                                                                      >  1:418174/1‑250 (MQ=255)                                                                                                                                                                     aCTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTgacgac                                                                          <  2:247103/251‑1 (MQ=255)                                                                                                                                                                          cTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTg                                                                     >  2:321635/1‑251 (MQ=255)                                                                                                                                                                                   ttACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATgccg                                                            >  1:8781/1‑251 (MQ=255)                                                                                                                                                                                     aCATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGGTTTTGCAGCAGTAGAGTCAGAGTTGAAGCAGTAACGTTAACAGGAAGTTGATATTGCTGACGACGGCTGAAAAGgccgc                                                           <  2:314489/250‑1 (MQ=255)                                                                                                                                                                                     aCATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCAATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATgccgcc                                                          >  1:354708/1‑251 (MQ=255)                                                                                                                                                                                       aTCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTTCGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGccc                                                         >  2:182841/1‑250 (MQ=255)                                                                                                                                                                                             aaCGACACGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTGGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGa                                                   >  1:136342/1‑250 (MQ=255)                                                                                                                                                                                              ccGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAATGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGAAt                                                 >  2:83701/2‑251 (MQ=255)                                                                                                                                                                                                 aCAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGAATTAc                                              <  2:249590/251‑1 (MQ=255)                                                                                                                                                                                                     gATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGAATTACCGtt                                          <  2:354708/251‑1 (MQ=255)                                                                                                                                                                                                            ggCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGAATTACCGTTAAGGCTg                                   >  1:589368/1‑251 (MQ=255)                                                                                                                                                                                                                 tATCACCTTCCATATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATg                                                               >  1:427292/1‑218 (MQ=255)                                                                                                                                                                                                                 tATCAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATg                                                               <  2:427292/218‑1 (MQ=255)                                                                                                                                                                                                                    cAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTTCGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGAATTACCGTTAAGGCTGTTTTATc                            <  1:182841/250‑1 (MQ=255)                                                                                                                                                                                                                    cAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGAATTACCGTTAAGGCTGTTTTATCg                           <  1:54595/251‑1 (MQ=255)                                                                                                                                                                                                                    cAACTTCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAAGGCCGCCCAGACGAATTACCGTTAAGGCTGTTTTATCg                           <  1:332273/251‑1 (MQ=255)                                                                                                                                                                                                                        ttCCAGATTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGAATTACCGTTAAGGCTGTTTTATCGCttt                       >  1:170122/1‑251 (MQ=255)                                                                                                                                                                                                                           cagattcagattCGGGACAGAGCTGATACCGTTCAGCTTCCCCAGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATCAACCACTTCCATATCATGCAGATTTAACGGCTTTTGCAGCAGAAGAGTCAGAGTGGACGCAGTAACGTTAACAGGCAGTTGATACTGCGGCAGACGGCTGAAAATGCCGCCCAGACGAATTACCGTTAAGGCTGTTTTATCGCTttgtt                    >  2:42910/1‑251 (MQ=255)                                                                                                                                                                                                                              aTTCAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGAATTACCGTTAAGGCTGTTTTATCGCTTTGTtgct                 <  1:388492/251‑1 (MQ=255)                                                                                                                                                                                                                                 cAGTTTCTGGGCAAAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGAATTACCGTTAAGGCTGTTTTATCGCTTTGTTgctgct              >  1:497869/1‑251 (MQ=255)                                                                                                                                                                                                                                        tGGGCACAGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTAAGCCACTGTTCCAGCGCGCTACGCTCAACGGAGATATGAACCAATTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGCAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGAATTACCGTTAAGACTGTTTTATCGCTTTGTTGCTGCTGGANTTg       >  2:595386/1‑251 (MQ=255)                                                                                                                                                                                                                                            cAAAGCTGATATCGTGGAGTTGACCGCGAATTTCGCCGCCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTTCAGCAGTAGAATCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGTCGACGGCTGAAGATGCCGCCCAGACGAACTACCGTTAAGGCTGTTTTATCGCGTTGTTGCTGCTGGAGTGGCAgg   <  2:578677/251‑1 (MQ=255)                                                                                                                                                                                                                                             aaaGCTGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGAATTGCCGTTAAGGCTGTTTTATCGCTTTGTTGCTGCTGGAGTTGCAGGt  <  1:434300/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      AGGCTGGCTTCAATGCGACCTAACCAGTCGCCAGGGATAAACAGACATTTTTGCTGTTCGCTGAATAAAGCGTGTTGCTTGCGCCAGTCGCTTTCAAGCTCTTCCAGTTGTTGTTTTATTTCACCAGGCAGACCTTCAGCCGATGAACCGGGAAGTGCCAGCGTACTGCCTTGTAAACTTACATCGCGAACGACAAGATGTTGGGCGCTATCAACTTCCAGATTCAGTTTCTGGGCAAAGCCGATACCGTTCAGTTTACCGCGAATTTCACCACCTTTGCTCAGCCACTGTTCCAGCGCGCTACGCTCAAAGGAGATATGAACCACTTCCATATCATGCAGTTTTAACGGTTTTTGCAGCAGTAGAGTCAGAGTGGAAGCAGTAACGTTAACAGGAAGTTGATACTGCTGACGACGGCTGAAAATGCCGCCCAGACGAATTACCGTTAAGGCTGTTTTATCGCTTTGTTGCTGCTGGAGTTGCAGGT  >  NC_000913/3929640‑3930126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑