Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,086,382 C→T W46* (TGG→TGA)  pgaD ← poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,086,3820CT97.8% 145.8 / NA 45W46* (TGG→TGA) pgaDpoly‑N‑acetyl‑D‑glucosamine synthase subunit PgaD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (18/26);  minor base G (1/0);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGC  >  NC_000913/1086146‑1086621
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
aTTTGTCCCTGTCTGGTTAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAATCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAGTAACCCGt                                                                                                                                                                                                                                   <  1:124326/251‑1 (MQ=255)
aTTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCg                                                                                                                                                                                                                                    <  2:21007/250‑1 (MQ=255)
   tGCCGCTGGCTGGAGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACGGCGCCCAGACAATTGACACGACGGCATTCGCCACTGCCAGCAAAAAATAGTACTGAAGTCGGCTTCTGGTCTCTCTTGGTCAGTAATATCCCGTca                                                                                                                                                                                                                                 <  2:116164/250‑1 (MQ=255)
   tGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTc                                                                                                                                                                                                                                  >  1:595115/1‑249 (MQ=255)
        ctggctggTGAAGTGTACGCTTATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTcagcag                                                                                                                                                                                                                             <  2:506989/249‑1 (MQ=255)
          tgctggTGAAGTGTACGCTCATCCTGTGGCTTTGTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTTCATGATGCTGCTGTTTTTGAAAACGCAGCGTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCATCAAAAAAGAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGAt                                                                                                                                                                                                                           <  1:388502/248‑1 (MQ=255)
          ggctggTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATcc                                                                                                                                                                                                                         <  1:228187/251‑1 (MQ=255)
              ggTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTcagc                                                                                                                                                                                                                               >  2:74695/1‑241 (MQ=255)
              ggTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTcagc                                                                                                                                                                                                                               <  1:74695/241‑1 (MQ=255)
                tGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCg                                                                                                                                                                                                                   >  1:645361/1‑251 (MQ=255)
                   agtttccGCTCATCCTGTGTCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAAt                                                                                                                                                                                                                <  2:515398/245‑1 (MQ=255)
                      gtACGCTCATCCTGTGGTTTTTTTGTAGTTGCGGATAGAGCTCATCAGGTAGTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAg                                                                                                                                                                                                             <  2:478050/251‑1 (MQ=255)
                          gCTCCTCCTGTTGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAata                                                                                                                                                                                                         <  2:501638/251‑1 (MQ=255)
                                   gtgGCTTTTTTGTAGTTGCTGATAGAGCTCATCATGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGaacaac                                                                                                                                                                                                 <  1:275391/250‑1 (MQ=255)
                                        ttttttGTAGGTGCTGATAGAGCTCCTCAGGTATTGATAACATCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGTTGTTTTTGAAAACGCAGCTTATTGTACTGCGCCCAGACAATTAACACGACGGCATTCCAAACTGCCAGCAAAAAATAGAACTGAAGTCGGCGTCTGGCCTCGCTTTATCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCaagt                                                                                                                                                                                           >  2:237460/1‑249 (MQ=255)
                                            ttGTAGTTGCTGATAGAGCTCATCAGGCATTGCTAAGCTCTCTGCATATTCTTGCGACGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTAGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGGCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATg                                                                                                                                                                                       >  2:439973/1‑251 (MQ=255)
                                                agnggCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTcc                                                                                                                                                                                    <  2:590787/246‑1 (MQ=255)
                                                 gTTGCTGTTAGAGGTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTGTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACTCAAATAATGTCcac                                                                                                                                                                                  <  2:441096/251‑1 (MQ=255)
                                                                            cTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAAcc                                                                                                                                                       >  2:488497/1‑251 (MQ=255)
                                                                                gCTCTCTGCATATTCTTGCGGCGTATATTGGTACGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGt                                                                                                                                                    >  1:194929/1‑250 (MQ=255)
                                                                                   ctctGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAAc                                                                                                                                                >  2:26141/1‑251 (MQ=255)
                                                                                       gCATTTTCTTGCGGCGTATATTGGTAGGCTTCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGTCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTa                                                                                                                                             <  1:243669/250‑1 (MQ=255)
                                                                                                tGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGAtt                                                                                                                                    <  1:488497/250‑1 (MQ=255)
                                                                                                    gcgTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCgg                                                                                                                               <  1:420354/251‑1 (MQ=255)
                                                                                                        atatTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCg                                                                                                                                                                                                                                    >  1:528165/1‑146 (MQ=255)
                                                                                                        atatTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCg                                                                                                                                                                                                                                    <  2:528165/146‑1 (MQ=255)
                                                                                                                       atgatgCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGt                                                                                                            >  1:7528/1‑251 (MQ=255)
                                                                                                                              tgctgTTTTTGAAAACGCAGCTTATTGTACATCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGTTTGTCGGGTCGTAATACTTAACTTGTTCATAtt                                                                                                     >  2:328997/1‑251 (MQ=255)
                                                                                                                                           aaCGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAAt                                                                                        >  1:51865/1‑251 (MQ=255)
                                                                                                                                                ggCTTATTATACAGCGGCCATACAATTAGCACGACAGCATTCGCCAATGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCACGATTTGTCAATAATAACCAGTAAGCAGATCCAGGGCGAATAAGAATATGAACCACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAACCGTACTGGTGATTGTCGGGTCGAAATAATTAAAATGTTCATATTAACCTATCAGAATccctc                                                                                   <  2:309618/250‑3 (MQ=255)
                                                                                                                                                 gCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCcgcg                                                                                  >  1:243827/1‑251 (MQ=255)
                                                                                                                                                  cTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGTCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAACTAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTACTAATTAAATTGTTCATATTAACCTCTCAGAATCAcgcg                                                                                  >  2:660755/1‑250 (MQ=255)
                                                                                                                                                           aaGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACtt                                                                        <  2:2075/250‑1 (MQ=255)
                                                                                                                                                                   aGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACg                                                                <  2:31942/251‑1 (MQ=255)
                                                                                                                                                                                gacgGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCttt                                                   >  1:299247/1‑251 (MQ=255)
                                                                                                                                                                                gacgGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCttt                                                   >  1:438480/1‑251 (MQ=255)
                                                                                                                                                                                          gCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCGCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGAGTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCa                                         <  1:318617/251‑1 (MQ=255)
                                                                                                                                                                                                            tAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATACTGTCCACAAGATGGTTGTGGCAACATAATCAACAAGTAAACGTACTGGTGATTGTCGGGCCGTAATAATTAAATTGTTCATATTAACCTCTCAGATTCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACCCGTGTAAATg                       >  1:619020/1‑251 (MQ=255)
                                                                                                                                                                                                                 ctgaacacaactcatGGCCTCGATTAGTCAGTAATAACCCGGCAGAAGCTCCTGGGCGAATCAGCATATGAACAACGCAAATAATGTCCACAAGATGGATGTGGCAACATAATCAACCAGAAAACGTACTGGTGATTGCCGGGTCGTAATAATACAATTGTTAATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCAAACGGGCGCGTTGCTTTTTAGGCATAAACATGACTCGTGTAAATGATAcc                  >  2:300342/15‑251 (MQ=255)
                                                                                                                                                                                                                    aaGTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCTCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTTTAAATGATACCAAt               <  2:92958/251‑1 (MQ=255)
                                                                                                                                                                                                                      gTCGGCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGt             <  1:472234/251‑1 (MQ=255)
                                                                                                                                                                                                                          gCTTCTGGCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGtt         <  1:435245/251‑1 (MQ=255)
                                                                                                                                                                                                                                ggCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCa     <  1:223794/249‑1 (MQ=255)
                                                                                                                                                                                                                                ggCCTCGCTTTGTCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCa     <  1:147120/249‑1 (MQ=255)
                                                                                                                                                                                                                                 gCCTCGCTTTGTAAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGc  >  2:37615/1‑251 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
ATTTGCCCCTGGCTGGTGAAGTGTACGCTCATCCTGTGGCTTTTTTGTAGTTGCTGATAGAGCTCATCAGGTATTGCTAAGCTCTCTGCATATTCTTGCGGCGTATATTGGTAGGCTGCATGATGCTGCTGTTTTTGAAAACGCAGCTTATTGTACAGCGCCCAGACAATTAACACGACGGCATTCGCCACTGCCAGCAAAAAATAGAACTGAAGTCGGCTTCTGGCCTCGCTTTGCCAGTAATAACCCGTCAGCAGATCCATGGCGAATAAGAATATGAACAACGCAAATAATGTCCACAAGATGGTTGTGGCAACATAATCAACCAGTAAACGTACTGGTGATTGTCGGGTCGTAATAATTAAATTGTTCATATTAACCTCTCAGAATCCCGCGATCGGGACTTACCCAACGGGCGCGTTGCTTTTTAGGCATCAACATGACTCGTGTAAATGATACCAATGTCGTTGCCAGGC  >  NC_000913/1086146‑1086621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑