Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,232,133	C→T	T367T (ACG→ACA)	lysC ←	aspartate kinase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,232,133	0	C	T	98.1%	177.7 / NA	53	T367T (ACG→ACA)	lysC	aspartate kinase III
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (30/22);  minor base G (1/0);  total (31/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCA  >  NC_000913/4231894‑4232379                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       aaaTTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGCCACAGTGCGGAAAGCTCCAACAGCAGAGATTGTGTCAGCAACGt                                                                                                                                                                                                                                             >  1:298210/1‑251 (MQ=255) aaaTTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGt                                                                                                                                                                                                                                             >  1:108810/1‑251 (MQ=255)       cTATGCAATTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATGACAAATCATGCGAATGTTGAACGGTTCCAGTACGCAGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGACAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGcc                                                                                                                                                                                                                                       >  2:566537/1‑251 (MQ=255)         aTGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCa                                                                                                                                                                                                                                      <  1:633541/250‑1 (MQ=255)                 tttGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGt                                                                                                                                                                                                                              <  1:458130/250‑1 (MQ=255)                  ttGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGtt                                                                                                                                                                                                                             <  1:185960/250‑1 (MQ=255)                          ccTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGCTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCgg                                                                                                                                                                                                                      >  2:393811/1‑249 (MQ=255)                           cTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCggtgg                                                                                                                                                                                                                   >  2:173681/1‑251 (MQ=255)                           cTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACATCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACACGACACAGAGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCggtgg                                                                                                                                                                                                                   >  1:276235/1‑251 (MQ=255)                                    aTCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAggg                                                                                                                                                                                                          >  1:482805/1‑251 (MQ=255)                                       ttCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGtt                                                                                                                                                                                                        >  1:315691/1‑250 (MQ=255)                                                                     gCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTa                                                                                                                                                                         <  2:550839/251‑1 (MQ=255)                                                                          aTGCGCCATAACAAATCATGCGAATGTTGACCGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGa                                                                                                                                                                                                                                 <  1:330353/190‑1 (MQ=255)                                                                          aTGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCAAACGACGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAACGCTCCATCAGCAGAGATTGGGTCAGCAACGTATCTCCAGTGGa                                                                                                                                                                                                                                 >  2:330353/1‑190 (MQ=255)                                                                           tGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCt                                                                                                                                                                    >  2:282094/1‑250 (MQ=255)                                                                               ccATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCg                                                                                                                                                                >  2:574452/1‑250 (MQ=255)                                                                                       aaTCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGACACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAAtattat                                                                                                                                                       >  2:124151/1‑251 (MQ=255)                                                                                                   gTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAgg                                                                                                                                            <  1:124151/250‑1 (MQ=255)                                                                                                        aCGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGaa                                                                                                                                                              <  1:354595/227‑1 (MQ=255)                                                                                                        aCGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGaa                                                                                                                                                              >  2:354595/1‑227 (MQ=255)                                                                                                         cGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGCAATATTATGCCGCGCGAGGATGCCg                                                                                                                                      >  1:306985/1‑250 (MQ=255)                                                                                                          ggTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGaa                                                                                                                                    <  2:482805/251‑1 (MQ=255)                                                                                                              ccAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACt                                                                                                                                <  2:242350/251‑1 (MQ=255)                                                                                                                     gCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCcgcg                                                                                                                          >  1:353357/1‑250 (MQ=255)                                                                                                                        gAATCCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACTCGTCCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCTCGAGGa                                                                                                                      <  2:501438/251‑1 (MQ=255)                                                                                                                            ccctcttcgccaactgcgtagtctgttGACAGGTCATTGCCAATCAACGCGGACAGCGCCAGACCTTCTGCCACCGCCACCCGACACAGGGCGGAAAGCTCCAGCAGCAGAGATTGTGTAAGCAACGTAACGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCGGACGTGGTGATTAAGGCTACCGAAATATTATGCCGCGCGAGGAGGCCGAAAACTGCCGCGAGCAAAcc                                                                                                                  <  2:641191/226‑1 (MQ=255)                                                                                                                                ctTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGtt                                                                                                                                                                                                        <  2:468768/161‑1 (MQ=255)                                                                                                                                ctTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGtt                                                                                                                                                                                                        >  1:468768/1‑161 (MQ=255)                                                                                                                                       aaCGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGCCCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATgca                                                                                                       <  2:220209/251‑1 (MQ=255)                                                                                                                                       aaCGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCAGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTAACGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATgc                                                                                                        >  2:519321/1‑250 (MQ=255)                                                                                                                                         cgccgcAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATgcagc                                                                                                     >  1:268409/1‑251 (MQ=255)                                                                                                                                          gccgcAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATgcagc                                                                                                     >  1:569471/1‑250 (MQ=255)                                                                                                                                           ccgcAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGGGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCat                                                                                                   >  2:70730/1‑251 (MQ=255)                                                                                                                                                          aGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCTCCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCaa                                                                                    <  1:70730/251‑1 (MQ=255)                                                                                                                                                             tCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGt                                                                                 >  1:283036/1‑251 (MQ=255)                                                                                                                                                                  gCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGc                                                                             >  2:342549/1‑250 (MQ=255)                                                                                                                                                                                    cgcCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGGTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGcgacga                                                           >  2:446296/1‑250 (MQ=255)                                                                                                                                                                                       cAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAgcg                                                       <  2:538460/251‑1 (MQ=255)                                                                                                                                                                                          aCCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGcc                                                     >  2:165587/1‑250 (MQ=255)                                                                                                                                                                                                     cctccaccTGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAATGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGACAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAAc                                         <  1:410082/251‑1 (MQ=255)                                                                                                                                                                                                        ccaccCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAgc                                      >  1:294483/1‑251 (MQ=255)                                                                                                                                                                                                                       cGGAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGCGGTATCAAGGGTTAATGCCACGCTCACTTCTGAAGTGGGGATTAAGTATACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAAACGCGACAATGCAGCATATTCAGGCTGTGCAACGGCGGCAGAGGCTGATTGCGACGAAGAGCCAGAGCCCGGAACAGCGGCGGAttgta                           >  1:285436/1‑244 (MQ=255)                                                                                                                                                                                                                        ggAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTTATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGttt                      <  2:358410/251‑1 (MQ=255)                                                                                                                                                                                                                        ggAAAGCTCCATCAGCAGAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGttt                      <  2:315691/251‑1 (MQ=255)                                                                                                                                                                                                                          aaaGCTCAATCAGCAGCGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTAGTATCAAGGGTTAATGCCACGCCCACTTCTGACGTGGGGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGCAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGGGAGCAGATTCTGAATGCGACGAACCGCCATAGCGCGGAAAACAGGCGGCATTTCAGtttt                     >  2:561647/1‑250 (MQ=255)                                                                                                                                                                                                                                     agcagAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTcc                                                                                                                             <  2:564200/135‑1 (MQ=255)                                                                                                                                                                                                                                     agcagAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGGCTACCGAAATAGTATGCCGCGCGATGATGCCTAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAg         <  2:298210/251‑1 (MQ=255)                                                                                                                                                                                                                                     agcagAGATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAAAAGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTcc                                                                                                                             >  1:564200/1‑135 (MQ=255)                                                                                                                                                                                                                                        agagATTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGGGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCACTTTTATTCCACACCAGCGt      <  2:381022/251‑1 (MQ=255)                                                                                                                                                                                                                                         gagaTTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGGATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGt      >  1:27653/1‑250 (MQ=255)                                                                                                                                                                                                                                           gaTTGTGTCAGCACCGTATCGCCAGTGGAGGTTGAACCGGTGGTAACAAGGGTTAATGCCACGCTCACTTCTGACGTGGCGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGAtt                                                                  >  2:604314/1‑188 (MQ=255)                                                                                                                                                                                                                                           gaTTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGAtt                                                                  <  1:604314/188‑1 (MQ=255)                                                                                                                                                                                                                                            aTTGTGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCAAGCTCACTTCTGAAGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTACGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGAGGAAGAGCCTGAGCGCGGAACAGCGGCGGGTTTTCCGTTTTCTTGCACACAAGCGTaccc  >  2:652975/1‑250 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       AAATTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGTGGAGGTTGAACCGGTGGTATCAAGGGTTAATGCCACGCTCACTTCTGACGTGGTGATTAAGTCTACCGAAATATTATGCCGCGCGAGGATGCCGAAAACTTCCGCGAGGAAACCGCGAGAATGCAGCATATTCAGGCTGTGCAAAGTGAGCAGAGTCTGATTGCGACGAAGCGCCAGAGCGCGGAACAGCGGCGGATTTTCAGTTTTATTGCACACCAGCGTACCA  >  NC_000913/4231894‑4232379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑