Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,944,191	G→A	A260T (GCC→ACC)	znuB →	Zn(2(+)) ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,944,191	0	G	A	97.8%	134.4 / ‑5.6	45	A260T (GCC→ACC)	znuB	Zn(2(+)) ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (13/31);  minor base C (0/1);  total (13/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

GATGAAATTTGTCGGTGCGTTGATTATTACTTCGTTGCTGATTATTCCTGCTGCTACTGCGCGTCGCTTTGCCCGCACGCCGGAACAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTG  >  NC_000913/1943962‑1944435                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     gATGAAATTTGTCGGTGCGTTGATTATTACTTCGTTGCTGATTATTCCTGCTGCTACTGCGCGTCGCTTTGCCCGCACGCCGGAACAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACTCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAac                                                                                                                                                                                                                                 <  2:51804/251‑1 (MQ=255)    gAAATTTGTCGGTGCGTTGATTATTACTTCGTTGCTGATTATTCCTGCTGCTACTGCGCGTCGCTTTGCCCGCACGCCGGAACAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAacac                                                                                                                                                                                                                               <  2:565041/250‑1 (MQ=255)     aaaTTTGTCGGTGCGTTGATTATTACTTCGTTGCTGATTATTCCTGCTGCTACTGCGCGTCGCTTTGCCCGCACGCCGGAACAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGGCCCGCGGGGGGGCTATGTGGGGGACTGTTATTTTTTTTCAGTATGATGAAAAAACAGACCAGCTAATCTATCGCTGAacaac                                                                                                                                                                                                                              >  1:538344/1‑248 (MQ=255)          tGTCGGTGCGTTGATTATTACTTCGTTGCTGATTATTCCTGCTGCTACTGCGCGTCGCTTTGCCCGCACGCCGGAACAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGt                                                                                                                                                                                                                         <  1:541083/250‑1 (MQ=255)               gTGCGTTGATTATTACTTCGTTGCTGATTATTCCTGCTGCTACTGCGCGTCGCTTTGCCCGCACGCCGGAACAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATg                                                                                                                                                                                                                   <  2:413013/251‑1 (MQ=255)                                     gCTGATGATTCCTGCTGCTACTGCGCGTCGCTTTGCCCGCACGCCGGAACAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGAc                                                                                                                                                                                             <  2:517252/251‑1 (MQ=255)                                           tattCCTGCTGCTACTGCGCGTCGCTTTGCCCGCACGCCGGAACAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACg                                                                                                                                                                                        <  2:175474/250‑1 (MQ=255)                                           tattCCTGCTGCTACTGCGCGTCGCTTTGCCCGCACGCCGGAACAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCCGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCt                                                                                                                                                                                           <  1:21918/247‑1 (MQ=255)                                                                 cGCTTTGCCCTCACGCCGGAACAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAGCCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTg                                                                                                                                                                 <  2:147187/251‑1 (MQ=255)                                                                     ttGCCCGCACGCCGGAACAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTa                                                                                                                                                              <  1:348555/250‑1 (MQ=255)                                                                               gCCGGAACAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACa                                                                                                                                                   <  1:244282/251‑1 (MQ=255)                                                                                     aCAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCTAACAGCGTCg                                                                                                                                             <  2:275099/251‑1 (MQ=255)                                                                                            gCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAgg                                                                                                                                      <  1:463275/251‑1 (MQ=255)                                                                                               ggTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGc                                                                                                                                     <  1:360171/249‑1 (MQ=255)                                                                                                gtgtCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCata                                                                                                                                  <  1:95196/251‑1 (MQ=255)                                                                                                           ttGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGc                                                                                                                        <  2:108670/250‑1 (MQ=255)                                                                                                                                   ggCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGATCGACCAATc                                                                                                <  2:285366/250‑1 (MQ=255)                                                                                                                                   ggCGGTTTAACCTTTTCCGCGGTTGACAATACGCCGGCGGGGCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCGACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATcc                                                                                               <  2:11143/251‑1 (MQ=255)                                                                                                                                                tttCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGGGGTGCTCTGTGCGGGCCTGGTTTTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTCTCGCTGAACCCACTTGTCGGATGCGGCGCGAGCGCCTTATCCGCACTACGGTTCGGGTTCTCTGGGAGGCCTGGTAAGACGCGAAAAGCGTCGCATCAGGGATATTGCCCGTGCCGGGTGCGGCGCGAGCGACCAATCCCACCTTCGGCAtt                                                                                  >  1:386019/1‑251 (MQ=255)                                                                                                                                                    cgcgGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTg                                                                              <  2:64299/251‑1 (MQ=255)                                                                                                                                                    cgcgGTTTACGATACGCCGGCGGGTCCGTCGGGGGTGCTATGTGCGGCACTGTTCTTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTTTCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAAACGCGAACAACGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGCCCAATCCGACTTACGGccattctg                                                                              >  1:607686/1‑244 (MQ=255)                                                                                                                                                       ggTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTggcg                                                                           <  2:660503/251‑1 (MQ=255)                                                                                                                                                                  cGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGAATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCa                                                                <  2:227703/251‑1 (MQ=255)                                                                                                                                                                      ggcggtgccggcGGTGGTGTTATGTGCCTCACTGTTAGTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATATATCGCTGAACTCATTTGTCGGATGCGGCGCGATCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCCGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGt                                                            <  2:123925/240‑1 (MQ=255)                                                                                                                                                                           gTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTc                                                       >  1:181504/1‑251 (MQ=255)                                                                                                                                                                               gTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTCATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACg                                                   >  1:224925/1‑251 (MQ=255)                                                                                                                                                                               gTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATATGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACg                                                   >  1:455606/1‑251 (MQ=255)                                                                                                                                                                                  ggtngtGCTATGTGCGGCTCTGTTATTGATTTGCAGTATGATGAAAAAGCAGACCAGCTAATCTAGCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTAACCGACCTACGTTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGTGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGGGCGAACATTCCGACTTACGGCAGTTCTGGCGGCGGTATGCTAAAGTAATTCCACGccc                                                <  2:604360/251‑1 (MQ=255)                                                                                                                                                                                      gtgCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCGGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGcgt                                            >  2:611217/1‑251 (MQ=255)                                                                                                                                                                                         gtATGTGCGGCACTGTTGTTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGTCCGcgtcgt                                         <  2:648042/250‑1 (MQ=255)                                                                                                                                                                                            tgtgCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGtcgtcg                                       <  2:577978/250‑1 (MQ=255)                                                                                                                                                                                             gtgCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGCACACATTTGTCGGATGCGGCGCGCGCGCCTTATCCGACCGACGCTTCGGTATCTCTGGTATTCATGGTAAGACGCGAACAGCGACGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGAGACCAATCTGACTTACGGCATTTCTCACGGCGTAATCCCAAAGAGATTCCACGCGCACGTCGTCGcc                                     >  2:547966/1‑251 (MQ=255)                                                                                                                                                                                             gtgCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGcc                                     <  1:316493/251‑1 (MQ=255)                                                                                                                                                                                               gCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCAt                                   <  2:219714/251‑1 (MQ=255)                                                                                                                                                                                                cGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATa                                  <  2:611904/251‑1 (MQ=255)                                                                                                                                                                                                       gTTATTTATGCTCAGTATGATGAAAAAGCAGCCCAACTAATCTATCGCTGAACACTTTTGTCGTATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGGAGTCCTTGTAATACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATTCGGCGCGAGCGACCATTCCGACTTACGGCATTTCTGGCGGCGTTATGCCCAAGTGTTTCCGCGCCCGCGTCGTCGCCATACGCCCa                            <  2:498202/250‑1 (MQ=255)                                                                                                                                                                                                            ttattCTCAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCggg                      >  1:313783/1‑250 (MQ=255)                                                                                                                                                                                                                   cAGTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACg                 <  2:224925/249‑1 (MQ=255)                                                                                                                                                                                                                     gTATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGc             >  1:561200/1‑251 (MQ=255)                                                                                                                                                                                                                      tATGATGAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCTGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGCGCTTCCACGCCCGCGTCGTCGCCATACGCCCACGCggagtccgcgtc             >  2:11166/1‑240 (MQ=255)                                                                                                                                                                                                                       atgatgAAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCa            >  1:579670/1‑250 (MQ=255)                                                                                                                                                                                                                          atgaAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGACCAGCGTCGCATCAGGCCTATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGGCGCCAAACGCCCACGCGGTGTCCGCTGCaaaa         >  2:411808/1‑250 (MQ=255)                                                                                                                                                                                                                            gaAAAAGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGc      <  2:538344/251‑1 (MQ=255)                                                                                                                                                                                                                              aaaaGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCt    <  1:583895/251‑1 (MQ=255)                                                                                                                                                                                                                                aaGCAGACCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTg  <  2:628822/251‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     GATGAAATTTGTCGGTGCGTTGATTATTACTTCGTTGCTGATTATTCCTGCTGCTACTGCGCGTCGCTTTGCCCGCACGCCGGAACAGATGGCTGGTGTCGCTGTTTTGGTGGGGATGGTGGCAGTGACTGGCGGTTTAACCTTTTCCGCGGTTTACGATACGCCGGCGGGTCCGTCGGTGGTGCTATGTGCGGCACTGTTATTTATTCTCAGTATGATGAAAAAGCAGGCCAGCTAATCTATCGCTGAACACATTTGTCGGATGCGGCGCGAGCGCCTTATCCGACCTACGGTTCGGTATCTCTGGTAGTCCTGGTAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTG  >  NC_000913/1943962‑1944435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑