BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,062,669	C→T	A607V (GCC→GTC)	scpA →	methylmalonyl‑CoA mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,062,669	0	C	T	98.4%	403.3 / NA	125	A607V (GCC→GTC)	scpA	methylmalonyl‑CoA mutase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (59/63); minor base G (2/0); total (62/63)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

GCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGCCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGC  >  NC_000913/3062425‑3062910

gcCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTc                                                                                                                                                                                                                                             <  1:146442/251‑1 (MQ=255)

 ccACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTcc                                                                                                                                                                                                                                            <  1:53517/251‑1 (MQ=255)

   aCCCTGGGTGAAATTTCCGATGCGCTGGAAGTATCTCTCGACCGTGATCTGGTGCCAAGCCATTGTGTTCCCGGCGTGATTGCGCCAAGCTATCAACAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCCTTGTTGCGCAAACGGAGCAGTCCCTGGCCGACAATGGTCGTCGCCCGCTCTTTCTGATCGCGAAGAGGGGCCAGGATGGAAACGATCGCGGTGCGAAAGTGA‑TCGCCAGCGTCTATTCCGa                                                                                                                                                                                                                                          <  2:495091/251‑1 (MQ=255)

     ccTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCCGTCTGAGAAATCGGCCTCCGAGGTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACCATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCTCGGCGCGCAAGTGA‑TCGCCAGCGGCTATTCCGAtc                                                                                                                                                                                                                                        >  1:483740/1‑251 (MQ=255)

     ccTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGGTGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGAtc                                                                                                                                                                                                                                        >  2:523159/1‑251 (MQ=255)

        gggTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGTCAAGCCAGTGTGTTACCGGCGTGATTGCGCAGAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAATGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGAtctca                                                                                                                                                                                                                                     <  2:156960/251‑2 (MQ=255)

        gggTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCg                                                                                                                                                                                                                                     <  1:143473/251‑1 (MQ=255)

              aaTTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCg                                                                                                                                                                                                                               >  1:247145/1‑251 (MQ=255)

                  tCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACg                                                                                                                                                                                                                            <  2:479865/250‑1 (MQ=255)

                   ccGATTCGCTGGAAGTCGTTTTTGCCCGTGATCTGGTGCCAAGCCAGTGGGTTACCTGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCGCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGGCAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGt                                                                                                                                                                                                                           <  1:3414/250‑1 (MQ=255)

                   ccGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATTGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGGGG‑TCGCCAGTGTCTATTCCGCTCTCGGTTTCGACGt                                                                                                                                                                                                                           >  2:457266/1‑250 (MQ=255)

                   ccGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTa                                                                                                                                                                                                                          <  1:150114/251‑1 (MQ=255)

                   ccGAGGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATAGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGt                                                                                                                                                                                                                           <  2:638012/250‑1 (MQ=255)

                      aTGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGAt                                                                                                                                                                                                                       <  1:322118/251‑1 (MQ=255)

                        gcgcTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGG‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGAttt                                                                                                                                                                                                                     >  1:316121/1‑251 (MQ=255)

                        gcgcTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGAtt                                                                                                                                                                                                                      <  1:227008/250‑1 (MQ=255)

                          gcTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTaa                                                                                                                                                                                                                   <  2:205290/251‑1 (MQ=255)

                             ggAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGCTTGCGCAAAGCTATCATCAGTCTGACAAATCGGCCTCCCAATTCGCTGCCATTGTTGCGCAAACGGAGCAGGTCCTTGCCGCCACTTGTCGTCGCCCGCGCATTCTGATCGCTAAACTGGGCCAGGATGGACACGATAGCGGCGCGAAA‑CGACTCGCCAGCGGCTATTCCCATCTCGGTTTCGACGTAGAttttaacc                                                                                                                                                                                                                >  2:75477/1‑246 (MQ=255)

                              gAAGTCGCTTTCGACCGTTATCTGGTTCCAAGCCCGTGTGTTACCGTCGTGATTGCTCACAGCTATCATCACTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATTGTCTTCGCCCGCGCATTCTCATCGCTAAGATAGGCCAGGATGGACACGATCGCGGCGCGAAAGTTA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGccc                                                                                                                                                                                                               <  2:577911/251‑1 (MQ=255)

                                aGTCGCTTTCGACCGTCATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGTCACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCg                                                                                                                                                                                                              >  1:240660/1‑250 (MQ=255)

                                 gTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGTCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGAt                                                                                                                                                                                                            <  1:326110/251‑1 (MQ=255)

                                       ttCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTtc                                                                                                                                                                                                        <  1:261232/249‑1 (MQ=255)

                                       ttCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATGTAAGCCCGATGTtc                                                                                                                                                                                                        <  1:526756/249‑1 (MQ=255)

                                         cGACCGTTATCTTGTGCGAATCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCTGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCTCGAAAGTGA‑TCGCCAGCTTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTctct                                                                                                                                                                                                     <  2:562434/250‑1 (MQ=255)

                                           aCCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTaca                                                                                                                                                                                                  <  2:460104/251‑1 (MQ=255)

                                                      gTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCGCTACACCTGAAGAGAt                                                                                                                                                                                       <  1:247186/251‑1 (MQ=255)

                                                       tGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAag                                                                                                                                                                                           >  1:260957/1‑246 (MQ=255)

                                                       tGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAag                                                                                                                                                                                           >  1:286531/1‑246 (MQ=255)

                                                       tGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAag                                                                                                                                                                                           >  1:54878/1‑246 (MQ=255)

                                                        gCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATc                                                                                                                                                                                      <  1:135009/250‑1 (MQ=255)

                                                          cAAGCCAGTGAGTTACCGGCGTGATTGCGCAAAGATATCATCAGGCTGAGCAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGCTGGGCCAGGATGGACACGAACGCGGCGCGAAAGTGG‑ACGCCAGCGTCTATTCCGATCTCGGTTTCGGAGGAGATTTAAGCCCGATGTTCTCTAAACCTGAAGAGATcgcc                                                                                                                                                                                   >  1:51455/1‑251 (MQ=255)

                                                                     gtTACCGGGGTGATTGCGCAAAGCTAGCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGt                                                                                                                                                                        <  1:621839/251‑1 (MQ=255)

                                                                         ccGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGaa                                                                                                                                                                    <  1:335339/251‑1 (MQ=255)

                                                                                 aTTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGt                                                                                                                                                            <  1:537191/251‑1 (MQ=255)

                                                                                   tGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTc                                                                                                                                                          >  1:149708/1‑251 (MQ=255)

                                                                                    gcgcAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTc                                                                                                                                                          >  1:536736/1‑250 (MQ=255)

                                                                                       cAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGt                                                                                                                                                      >  1:292235/1‑251 (MQ=255)

                                                                                        aaaGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTtct                                                                                                                                                                                                       >  1:281237/1‑201 (MQ=255)

                                                                                        aaaGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTtca                                                                                                                                                                                                       <  2:281237/201‑2 (MQ=255)

                                                                                        aaaGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCAAGGCTGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGACTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCATGGCCGTAGAAAACGACGTTCACGt                                                                                                                                                      >  1:399532/1‑250 (MQ=255)

                                                                                              atcatcAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGGTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGGAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGgc                                                                                                                                               <  1:467740/251‑1 (MQ=255)

                                                                                               tcatcaGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGCAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGgcg                                                                                                                                              >  1:327601/1‑251 (MQ=255)

                                                                                                catcaGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCCCGTAGTGGgcgc                                                                                                                                             >  2:428285/1‑251 (MQ=255)

                                                                                                 atcaGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGgcgc                                                                                                                                             >  1:21103/1‑250 (MQ=255)

                                                                                                    aGTCTGAGAAATCGGCCTCCGAGTTCGATGACATTGTTGCGCAAACGGATCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCTCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATcc                                                                                                                                         <  1:499435/251‑1 (MQ=255)

                                                                                                           gaAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTgg                                                                                                                                  <  1:457266/251‑1 (MQ=255)

                                                                                                             aaTCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACATTGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACTCGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGTTGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCt                                                                                                                                <  2:86076/251‑1 (MQ=255)

                                                                                                              aTCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTg                                                                                                                               >  1:355784/1‑251 (MQ=255)

                                                                                                                cgtccTCCGAGGTGGATGCCTTGGTTGCGCAAACGGAGCAGTGCCTTGCCGACAATGGGCGTCGCCCGCGCATTCTGAGCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTGCACTACACCTGAAGAGATGGCCCGCCTTGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGcc                                                                                                                             <  1:616515/248‑1 (MQ=255)

                                                                                                                 ggCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCg                                                                                                                            >  1:351924/1‑251 (MQ=255)

                                                                                                                   ccTCCGAGGTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGTCGCGAAAGAGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGAGGGCGCATCCTCACTGGCTGCCggc                                                                                                                          >  2:384560/1‑250 (MQ=255)

                                                                                                                    cTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTc                                                                                                                         >  1:215107/1‑251 (MQ=255)

                                                                                                                       cGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCAt                                                                                                                       >  1:28188/1‑250 (MQ=255)

                                                                                                                            tCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGAtctc                                                                                                                                                                                                                                      <  1:225679/134‑1 (MQ=255)

                                                                                                                            tCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGAtctc                                                                                                                                                                                                                                      >  2:225679/1‑134 (MQ=255)

                                                                                                                               aTGCCATTGTTGCGCAAACGGAGCAGTTCCTTGACGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTg                                                                                                              >  1:540271/1‑251 (MQ=255)

                                                                                                                                 gCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGAt                                                                                                            <  2:54878/251‑1 (MQ=255)

                                                                                                                                 gCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGAt                                                                                                            <  2:286531/251‑1 (MQ=255)

                                                                                                                                    aTTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATccc                                                                                                         >  2:245999/1‑251 (MQ=255)

                                                                                                                                       gttgCGCAAACGGAGCAGTTCATTGACGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAACCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTCCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCCTACAACGCTGATCCCggc                                                                                                      >  2:615035/1‑250 (MQ=255)

                                                                                                                                        ttgCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGa                                                                                                      <  2:351924/250‑1 (MQ=255)

                                                                                                                                          gcgcAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACt                                                                                                   <  1:8180/251‑1 (MQ=255)

                                                                                                                                             cAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGt                                                                                                >  1:108766/1‑251 (MQ=255)

                                                                                                                                               aaCGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCg                                                                                              >  1:365605/1‑251 (MQ=255)

                                                                                                                                                 cGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGaa                                                                                            <  2:247145/251‑1 (MQ=255)

                                                                                                                                                 cGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGa                                                                                             >  1:546861/1‑250 (MQ=255)

                                                                                                                                                  ggAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGa                                                                                             >  2:572732/1‑249 (MQ=255)

                                                                                                                                                   gAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCAAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAgc                                                                                          >  1:422241/1‑251 (MQ=255)

                                                                                                                                                       aGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTg                                                                                      >  2:168833/1‑251 (MQ=255)

                                                                                                                                                          tCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGaa                                                                                    <  1:558930/250‑1 (MQ=255)

                                                                                                                                                              tGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAAt                                                                               >  1:252385/1‑251 (MQ=255)

                                                                                                                                                              tGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGATAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATATCGGTTTCGACGTAGATTTAAGACCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAAt                                                                               >  2:189803/1‑251 (MQ=255)

                                                                                                                                                                  gACAATGGTCATCGCACGCGCATTCTGATCGCTAAGATGGGCCAGGATGGCCACGATGGCGACGCGAAAGGGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTAACGTAGTGGGCGCATCCTCACTGGCTGACGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGATGAAAAAATgggg                                                                           >  2:81562/1‑251 (MQ=255)

                                                                                                                                                                   aCAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATggggg                                                                          >  2:564323/1‑250 (MQ=255)

                                                                                                                                                                    cAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGAAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAACACGACGTTCACGTAGTGGGCGCATCCTCACTGGATGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGAc                                                                         >  2:249672/1‑251 (MQ=255)

                                                                                                                                                                      aTGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGAcgc                                                                       >  1:435336/1‑251 (MQ=255)

                                                                                                                                                                           cgtcgCCCGCGCATTCTGATCGCCAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAg                                                                   <  1:103636/250‑1 (MQ=255)

                                                                                                                                                                              cgCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACCCCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGatat                                                               <  1:75477/251‑1 (MQ=255)

                                                                                                                                                                              cgCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGatat                                                               >  2:294377/1‑251 (MQ=255)

                                                                                                                                                                               gcccgtgcattcgtacgacgaaGATGGGCGAGGATGTACCCGGTCGTGGCGCGAAAGTTC‑TCGCCATCCTCTATTCCCACCTCGGTTCCAACTAAAATTTACCCCCGATGTCTTCTCCACCTGAAGGGGTGGCCCGCCGGGCCATAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTACCGGTCATAAAACGCGGATCGCTGTACTTGGCTAAGGGCTGAAAAAATGGGGAGGCGAAGatata                                                              <  2:117927/231‑2 (MQ=255)

                                                                                                                                                                                 ccGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACTCCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGtt                                                                                                                                                           <  2:604634/156‑1 (MQ=255)

                                                                                                                                                                                 ccGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGtt                                                                                                                                                           >  1:604634/1‑156 (MQ=255)

                                                                                                                                                                                 ccGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTg                                                            >  2:275785/1‑251 (MQ=255)

                                                                                                                                                                                  cgcgCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGc                                                           <  2:260957/251‑1 (MQ=255)

                                                                                                                                                                                      cATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTgg                                                       >  1:549049/1‑251 (MQ=255)

                                                                                                                                                                                                       ggggCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAGAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCAtt                                      <  2:245066/250‑1 (MQ=255)

                                                                                                                                                                                                         ggCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGAGCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTcc                                    <  2:327714/251‑1 (MQ=255)

                                                                                                                                                                                                          gCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTccg                                   >  1:335230/1‑251 (MQ=255)

                                                                                                                                                                                                          gCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTACGATCTTGGATTCGACGTAGATTTAAGACCGATGTTCTCTACACCTGAAGAGATCCCCCGCCTGGCAGTAGAAAACGACGTTCACGTAGAGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACAGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGCGGCGACAttacg                                   >  1:381684/1‑248 (MQ=255)

                                                                                                                                                                                                            cAGGATGGACACGAGCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTTTTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTccgcc                                 <  1:634606/251‑1 (MQ=255)

                                                                                                                                                                                                             aGGATGGACACGATCGCGGCGCGAAAGTGA‑TAGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGACATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGTGTGGCGTCATTcagccg                                >  1:232541/1‑251 (MQ=255)

                                                                                                                                                                                                                 tGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAgg                            >  1:13254/1‑251 (MQ=255)

                                                                                                                                                                                                                 tGGACACGATCACGGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTGTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGATGCCGGGCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGGGGCGTCATTCCGCCGCAgg                            >  1:253974/1‑251 (MQ=255)

                                                                                                                                                                                                                          gcgcgGCGCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCt                   <  2:335230/250‑1 (MQ=255)

                                                                                                                                                                                                                           cggcgcgcGAAAGTGA‑CCGCCGGCGTCTATTCCGATCTCGGTTGCGACGTAGAGTTAAGCCCGATGTTCTCTACACCTGAAGAGAGCCCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGTGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGGCGAAGCGCTGAAAAAATGGGGCCGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGTCtt                  <  2:232541/247‑1 (MQ=255)

                                                                                                                                                                                                                             cggcgCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTcc                >  2:555933/1‑251 (MQ=255)

                                                                                                                                                                                                                             cggcgCGAAAGTGA‑TCGCCAGCGTCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTcc                >  2:346065/1‑251 (MQ=255)

                                                                                                                                                                                                                                       gTGA‑TCGCCAGCTTCTTTTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAgcg      <  2:355784/251‑1 (MQ=255)

                                                                                                                                                                                                                                        ggA‑TCGCCAGCGTCTATTCCGACCTCGGTTTCGAGGTAGATTTAAGCCCGATGTTCTCTACACCGGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAgcgc     <  2:540271/250‑1 (MQ=255)

                                                                                                                                                                                                                                            ccGCCAGCTTCTATCCCTATCTCGGTTTCAACGTAGATTTAAGCCCGATTTTCTCTACCCCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCgcggc  <  2:506900/250‑1 (MQ=255)

GCCACCCTGGGTGAAATTTCCGATGCGCTGGAAGTCGCTTTCGACCGTTATCTGGTGCCAAGCCAGTGTGTTACCGGCGTGATTGCGCAAAGCTATCATCAGTCTGAGAAATCGGCCTCCGAGTTCGATGCCATTGTTGCGCAAACGGAGCAGTTCCTTGCCGACAATGGTCGTCGCCCGCGCATTCTGATCGCTAAGATGGGCCAGGATGGACACGATCGCGGCGCGAAAGTGA‑TCGCCAGCGCCTATTCCGATCTCGGTTTCGACGTAGATTTAAGCCCGATGTTCTCTACACCTGAAGAGATCGCCCGCCTGGCCGTAGAAAACGACGTTCACGTAGTGGGCGCATCCTCACTGGCTGCCGGTCATAAAACGCTGATCCCGGAACTGGTCGAAGCGCTGAAAAAATGGGGACGCGAAGATATCTGCGTGGTCGCGGGTGGCGTCATTCCGCCGCAGGATTACGCCTTCCTGCAAGAGCGCGGC  >  NC_000913/3062425‑3062910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`