Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,004,371	C→T	L109L (CTG→CTA)	yigI ←	putative thioesterase YigI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,004,371	0	C	T	100.0%	90.9 / NA	30	L109L (CTG→CTA)	yigI	putative thioesterase YigI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/21);  total (9/21)

AAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCACACCAGACCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGT  >  NC_000913/4004125‑4004594                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                aaaataaGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTAGTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGa                                                                                                                                                                                                                              <  1:592221/250‑1 (MQ=255)                agCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGa                                                                                                                                                                                                                  >  2:401216/1‑247 (MQ=255)                 gctattAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCATCCTCCCATATAGGTGGCGGTGGCACTTGCAATATAAAGCTGTTCTTCATTGTGTAATGCAACGCGGGCGACGGCGACTTTAGTGCCTGCACGCAACAGGTTACTAGTAGCAGTAAAACGCTCGCGCCTGCCTGGGCGTAGATAATCAACGCCAAGAta                                                                                                                                                                                                             <  2:8713/246‑2 (MQ=255)                       aaTACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGt                                                                                                                                                                                                       >  1:217994/1‑251 (MQ=255)                              ccTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTTTAATTCAACGCGGGCGACGTCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATc                                                                                                                                                                                                <  1:71651/251‑1 (MQ=255)                              ccTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATc                                                                                                                                                                                                >  2:282212/1‑251 (MQ=255)                                  ccGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTTGGCGTAGATAATCAACGCTAAGATCaa                                                                                                                                                                                                           <  2:473914/236‑1 (MQ=255)                                  ccGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCaa                                                                                                                                                                                                           >  1:473914/1‑236 (MQ=255)                                               gCATCCATCGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGc                                                                                                                                                                                <  1:282212/250‑1 (MQ=255)                                                     atcGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTc                                                                                                                                                                         <  1:272074/251‑1 (MQ=255)                                                        gggAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGACTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGCAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATc                                                                                                                                                                      >  1:379462/1‑251 (MQ=255)                                                                 cATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGAt                                                                                                                                                             <  1:108287/251‑1 (MQ=255)                                                                         aTTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGt                                                                                                                                                     >  1:608542/1‑251 (MQ=255)                                                                                                          tCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAgggaattctcaagccacacaaac                                                                                                                    >  1:414959/1‑230 (MQ=255)                                                                                                           cAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCACACCAGAc                                                                                                                    <  1:401216/250‑1 (MQ=255)                                                                                                           cAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGGTTAGGTGCTTCCCACGccccccaccac                                                                                                                   >  2:484671/1‑241 (MQ=255)                                                                                                              ccTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCACACCAGACcggc                                                                                                                <  2:49950/251‑1 (MQ=255)                                                                                                              ccTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGGTAAgggaatttccccccacccccaacccgc                                                                                                                >  1:570139/1‑226 (MQ=255)                                                                                                                 aCCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCACACCAGACCggcggc                                                                                                             <  1:64012/251‑1 (MQ=255)                                                                                                                                        ggCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCACACCAGACCGGCGGCGACATCCAGCGCCGACGCAATg                                                                                       <  2:570139/250‑1 (MQ=255)                                                                                                                                            atatAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCACACCAGACCGGCGGCGACATCCAGCGCCGACGCAATGAcccc                                                                                  <  2:250654/251‑1 (MQ=255)                                                                                                                                              ataAAGCTGTTCGTCATTGTGTAATTCAACGCTGGCGACTGCGACTTTATGGCCTGCCCGCGACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTACGGTCCTTCCCACGCACTCCAGACCGGCGGCGTCATCCAGCTCCGACGCAATGACCccg                                                                                 <  2:614244/250‑1 (MQ=255)                                                                                                                                                           tctttttttAATTCAACGCGGGCGACGGCAACTTTATTGCCTGCACGCAACAGGCTCCTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCACACCAGACCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAAt                                                                      <  1:469983/240‑1 (MQ=255)                                                                                                                                                                    aaTTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCACACCAGACCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCa                                                          <  1:355771/251‑1 (MQ=255)                                                                                                                                                                                     ggcgacTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCACACCAGACCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTg                                         <  1:171351/251‑1 (MQ=255)                                                                                                                                                                                        gacTTTATTGCCTGCACGCAACAGACTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTATTTCATCTTCACTGATGGTTTCGTGTCGGGTTAAGGTACTTCCCACGCCCACCAGACCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCt                                      <  1:359536/251‑1 (MQ=255)                                                                                                                                                                                           tttATTGCCTGCACGCAACAGGCTCCTAGTACCATTAAACCGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCACACCAGACCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGa                                    <  2:424954/250‑1 (MQ=255)                                                                                                                                                                                                               ggCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGTAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCACACCAGACCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCg               <  2:317168/251‑1 (MQ=255)                                                                                                                                                                                                                      angagCAGTAAAACGCTCGCCCCTGCCTGGGGGTAGATAATCAACGCGAAGTTCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCACACCAGACCGGCGGCGACATCCAGCGCCGACGCAATGCCCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAActc          <  2:608542/246‑1 (MQ=255)                                                                                                                                                                                                                            aGTAAAACGCTAGACCCTGCCTGGTGGTAGATAATAAACGCGAAGATCAAGGGTCCCCAGCCGCGATAGCTGCGGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCACACGCACACCAGACCGGCGGCGAAATACAGCGCCGACGAAATGACCCCGCCGTGCAAAATGCTGTGCGCCCAGTTGCCCACCATCATTGGCGGATTTTTAAAGGCCAGCGGTGCGAACTCTTTTTCGt  <  1:208621/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                AAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTTAATTGGTAACAGCAGTTTACCTGCTTTTATGTCTTCAACCTACCATATAGGTGGCGGTGGCACTGGCAATATAAAGCTGTTCTTCATTGTGTAATTCAACGCGGGCGACGGCGACTTTATTGCCTGCACGCAACAGGCTACTAGTAGCAGTAAAACGCTCGCCCCTGCCTGGGCGCAGATAATCAACGCGAAGATCAATGGTCCCCATCCGCGATAGCCGCTGGCGTAGTTCATCTTCACTGATGGTTTCGTGGCGGGTTAAGGTACTTCCCACGCACACCAGACCGGCGGCGACATCCAGCGCCGACGCAATGACCCCGCCGTGCAAAATGCTTTGCGCCCAGTTGCCCACCATCATTGGCTGATTTTTAAAGGCCAGCTGTGCGAACTCTTTTTCGT  >  NC_000913/4004125‑4004594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑