Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	972,831	G→A	P191S (CCG→TCG)	elyC ←	envelope biogenesis factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	972,831	0	G	A	100.0%	184.5 / NA	55	P191S (CCG→TCG)	elyC	envelope biogenesis factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/33);  total (22/33)

ACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCA  >  NC_000913/972585‑973076                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       aCGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACCCTTGCCGGGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGAGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGa                                                                                                                                                                                                                                                    <  1:328361/251‑1 (MQ=255)       tAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGa                                                                                                                                                                                                                                               <  2:76970/250‑1 (MQ=255)        aGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGAt                                                                                                                                                                                                                                              <  2:371213/250‑1 (MQ=255)             gcgcAACTCCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGCACCCTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAAAACGATCGATATGCATCAACCAGACCGGAGAAGGGGTTGCCCGTTCCCACGGGTTAAGCGGCGAGTGGGTGGCCAACTGATTCGCCGGGGCTGGTAGCGGGTTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCgg                                                                                                                                                                                                                                          >  2:35176/1‑249 (MQ=255)               gcAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGt                                                                                                                                                                                                                                      <  1:505338/251‑1 (MQ=255)                cAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTc                                                                                                                                                                                                                                     >  1:546972/1‑251 (MQ=255)                               aTCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAggg                                                                                                                                                                                                                      <  1:194315/251‑1 (MQ=255)                                     aTCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCa                                                                                                                                                                                                                <  1:326203/251‑1 (MQ=255)                                                         ccTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGAtgt                                                                                                                                                                                                                                                 <  1:348489/198‑1 (MQ=255)                                                         ccTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGAtgt                                                                                                                                                                                                                                                 >  2:348489/1‑198 (MQ=255)                                                          cTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCActgcg                                                                                                                                                                                           >  2:523190/1‑250 (MQ=255)                                                             aCGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAggg                                                                                                                                                                                                                      <  2:103613/221‑1 (MQ=255)                                                             aCGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAggg                                                                                                                                                                                                                      >  1:103613/1‑221 (MQ=255)                                                                 aCCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGctt                                                                                                                                                                                    >  2:334437/1‑251 (MQ=255)                                                                        aGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttcttctt                                                                                                                                                                              <  1:80848/250‑1 (MQ=255)                                                                         gCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCg                                                                                                                                                                            <  2:240100/251‑1 (MQ=255)                                                                          caaCTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTGCACTGCTGCAGCTTCTTCTTCgg                                                                                                                                                                           <  2:267550/249‑1 (MQ=255)                                                                               gCCAGATACGGCCAAGCGTTTCATAGCCACCACGATCGCTTTGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATc                                                                                                                                                                       <  2:7568/250‑1 (MQ=255)                                                                               gCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATc                                                                                                                                                                       <  1:334437/250‑1 (MQ=255)                                                                                  aGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCTCCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCtttt                                                                                                                                                                   <  2:168769/251‑1 (MQ=255)                                                                                          ccAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGTTTGCCCGTTCCAACGGGTTCAGAGGCGAGTCGATGGGCAACTGATTCGACGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTAACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGAATCTTTTGGCAAATc                                                                                                                                                           >  1:599342/1‑251 (MQ=255)                                                                                                    cATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGAt                                                                                                                                                 <  2:495226/250‑1 (MQ=255)                                                                                                       aGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGTATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCTCTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAAtt                                                                                                                                             <  2:300814/251‑1 (MQ=255)                                                                                                           aaCACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCt                                                                                                                                         <  2:61130/251‑1 (MQ=255)                                                                                                               cGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTcgcg                                                                                                                                     <  1:413480/251‑1 (MQ=255)                                                                                                                 aTCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTcgcgcg                                                                                                                                   >  2:564183/1‑251 (MQ=255)                                                                                                                   cGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCgg                                                                                                                                  <  2:546972/250‑1 (MQ=255)                                                                                                                    gCTATGCATCAACCAGCACGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCTGCGAGTCGATGGCCACCGGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCTGTATCTTTTGGCAAACCCAGTGGT‑ATAATTTGTTCGCGCGGCa                                                                                                                                <  2:397645/251‑1 (MQ=255)                                                                                                                    gCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGc                                                                                                                                 >  1:657364/1‑250 (MQ=255)                                                                                                                       aTGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGACTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGc                                                                                                                             >  1:644828/1‑251 (MQ=255)                                                                                                                           aTCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGccc                                                                                                                           >  2:9451/1‑249 (MQ=255)                                                                                                                             cAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCg                                                                                                                       <  1:384401/251‑1 (MQ=255)                                                                                                                                ccAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGAtt                                                                                                                    >  1:655477/1‑251 (MQ=255)                                                                                                                                  aGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTgc                                                                                                                  >  2:533649/1‑251 (MQ=255)                                                                                                                                       ggcgacgggATTGCCCGTTACCACGGGGTAAAAGGCGAGTCGATGGCCCACTGATTCGCCGCGGCTGGTAACGGATTTAACCCTTCCAGCTGAAAAAAGAAAATCACCGGCGACAGATGTAATGCAGAGTGCACCAGCAGGAAAGGGGCGTAACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGACACATCCAG‑GGAGATAATTTGCTCGCGACGCACGCCCAGCGATTGCTCAAc                                                                                                             >  2:372390/7‑251 (MQ=255)                                                                                                                                           aaGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTg                                                                                                         <  1:642487/251‑1 (MQ=255)                                                                                                                                              ggATTGCCCGTTCCCACGGGTTAAGCGTCGAGTCGATGGCCAACTGATTCGCCGGTGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCa                                                                                                      <  2:659290/251‑1 (MQ=255)                                                                                                                                                   gCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTa                                                                                                  <  2:23878/250‑1 (MQ=255)                                                                                                                                                              cGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTTGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTAc                                                                                      <  1:92150/251‑1 (MQ=255)                                                                                                                                                               gggTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTcc                                                                                             >  2:262461/1‑243 (MQ=255)                                                                                                                                                               gggTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTcc                                                                                             <  1:262461/243‑1 (MQ=255)                                                                                                                                                               gggTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCGGGCACCTTCTTCCGCTGTACt                                                                                     <  2:655477/251‑1 (MQ=255)                                                                                                                                                                    aaGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCg                                                                                >  2:12053/1‑251 (MQ=255)                                                                                                                                                                     aGCGGCGAGACGATGGCCAACTGGTTCGCCGGGGCTGGGAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGt                                                                               >  2:412825/1‑251 (MQ=255)                                                                                                                                                                        ggcgAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTAtt                                                                            >  1:316583/1‑251 (MQ=255)                                                                                                                                                                               cGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAGCTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGtttt                                                                      <  1:363019/250‑1 (MQ=255)                                                                                                                                                                                     ccAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACg                                                               <  1:570464/251‑1 (MQ=255)                                                                                                                                                                                         cTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGGTTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTc                                                           >  1:506467/1‑251 (MQ=255)                                                                                                                                                                                                  cGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAACAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTTCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGa                                                     >  2:49665/1‑248 (MQ=255)                                                                                                                                                                                                  cGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGGTTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGa                                                  <  2:506467/251‑1 (MQ=255)                                                                                                                                                                                                                       cccTTCCTGCTGAAAAAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGCAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCTGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTGTTGATCCCGGAtttt                             <  2:251372/251‑1 (MQ=255)                                                                                                                                                                                                                        ccTTCCTGCTGAAACAAGATCATCGCCCGCGACAGATGTGATGCGGAGGTCAACAGCAGGAAAGGGGCGTCACAAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGCTCAGTTTTGATCCCGGATNTTc                            >  2:601083/1‑251 (MQ=255)                                                                                                                                                                                                                         cTTCCTGCTGAAAAACGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTcg                           >  1:523911/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaaaGATCATCGCCCGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGa               <  1:302385/251‑1 (MQ=255)                                                                                                                                                                                                                                                  ccGCGACAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGAGCCCGGATTTTCGCGCCATAAGCGAATACCttcattca  <  2:644828/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       ACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTTCCCACGGGTTAAGCGGCGAGTCGATGGCCAACTGATTCGCCGGGGCTGGTAGCGGATTTAACCCTTCCTGCTGAAAAAAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTCGGTATCTTTTGGCAAATCCAG‑GGTGATAATTTGCTCGCGCGGCACGCCCAGCGATTGCGCAACTCTGGCACCTACTTCCGCTGTACTCACCGTATTGGTTTTTGCTACGCCTCCCGTGAAGATCAGTTTTGATCCCGGATTTTCGCGCCATAAGCGAATACCTTCATTCA  >  NC_000913/972585‑973076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑