Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	969,683	C→T	L99F (CTT→TTT)	ycaQ →	interstrand DNA crosslink repair glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	969,683	0	C	T	100.0%	192.8 / NA	57	L99F (CTT→TTT)	ycaQ	interstrand DNA crosslink repair glycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/29);  total (28/29)

GCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTGGAGGATATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGCCGCATAAACGGCATCTGGAAGGTTTATTTACTGCCGGAAAGGTGATGGTGA  >  NC_000913/969447‑969914                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        gCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTGGAGGATATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGc                                                                                                                                                                                                                           >  1:219430/1‑251 (MQ=255)        aaacaaacCCCGCCGTCGAGCGTCGTTGGAGGATATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTg                                                                                                                                                                                                                     <  2:634601/250‑1 (MQ=255)                    ccgTCGAGCGTCGTTGGAGGATATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGaaaaa                                                                                                                                                                                                         >  2:472657/1‑250 (MQ=255)                      gTCGAGCGTCGTTGTAGGATATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGGTGCTGGATGATTCTCTTGCGCGTGTCGAATTAATGGAATACTGGGCGCATTAAGCCTGCTTTATTCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGCCCCCTGAAAAAATg                                                                                                                                                                                                      >  2:189137/1‑251 (MQ=255)                      gTCGAGCGTCGTTGGAGGATATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCTGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATg                                                                                                                                                                                                      <  2:352617/251‑1 (MQ=255)                         gAGCGTCGTTGGAGGATATTCCGGCACCGATCTCCCGCATGTCCTTGCTGCAAATCGTTACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCAAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTACTGGAATACTGGGCGAATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCAACGCATCCTGGCACCTGCAAAAATGGGc                                                                                                                                                                                                   >  2:154186/1‑251 (MQ=255)                         gAGCGTCGTTGGAGGATATTCCGGCAACGATCTCCCGCATGTCCTTGCTTCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGc                                                                                                                                                                                                   >  1:470973/1‑251 (MQ=255)                                                   aCGATCTCCCTCATGTTCTTACTGCATATCGATACCATCAATATTGTTCCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGAAAATTATCCTGCCCAGTGTCTGGATGAGTCTCTGTCGCGTGGCGAATTAATGGTATTCTTGTCGCATGTAACCTGCCTTATGCCGTGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCa                                                                                                                                                                         <  2:205067/251‑1 (MQ=255)                                                             gCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTCTCGTTTTATTCGCCACCGCAGGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAg                                                                                                                                                               <  2:92594/251‑1 (MQ=255)                                                             gCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTGTTCAGTAGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGCAGCAGGAACATg                                                                                                                                                                 >  2:326857/1‑249 (MQ=255)                                                              cATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAgg                                                                                                                                                              <  1:151344/251‑1 (MQ=255)                                                                tGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCTTATATGGTGCTTTTCAGTCGTCTGGGAACTTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGCACATGAGGCg                                                                                                                                                            >  1:409892/1‑251 (MQ=255)                                                                 gTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCg                                                                                                                                                            <  1:141199/250‑1 (MQ=255)                                                                  tCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGCATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCgg                                                                                                                                                           >  2:28596/1‑250 (MQ=255)                                                                    cTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGaa                                                                                                                                                         >  1:369672/1‑250 (MQ=255)                                                                      tgctgcAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCTGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAAt                                                                                                                                                       <  1:212630/250‑1 (MQ=255)                                                                                    aCCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCa                                                                                                                                        <  2:579934/251‑1 (MQ=255)                                                                                          aaTATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCatat                                                                                                                                  <  2:470973/251‑1 (MQ=255)                                                                                           atatTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATAtt                                                                                                                                 <  2:147376/251‑1 (MQ=255)                                                                                               tgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATg                                                                                                                             >  2:1585/1‑251 (MQ=255)                                                                                                                atCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACg                                                                                                             <  1:280293/250‑1 (MQ=255)                                                                                                                       gCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCg                                                                                                     <  2:219430/251‑1 (MQ=255)                                                                                                                             cAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGAtttt                                                                                                <  1:585894/250‑1 (MQ=255)                                                                                                                                tcgtcTGGGAAATTATACTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGc                                                                                            >  2:620580/1‑251 (MQ=255)                                                                                                                                    cTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGCTTTTGAGCATcc                                                                                        >  2:188077/1‑251 (MQ=255)                                                                                                                                      gggAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATcc                                                                                        <  2:544280/249‑1 (MQ=255)                                                                                                                                      gggAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTc                                                                                      >  1:280888/1‑251 (MQ=255)                                                                                                                                          aaTTATCCTGCCCAGTGGCTGGATGAGTCTATGGCGCGTGGCGCATTCCTGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCCCCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTa                                                                                   >  1:33888/1‑250 (MQ=255)                                                                                                                                                 cTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAggggc                                                                            >  2:375668/1‑247 (MQ=255)                                                                                                                                                 cTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGc                                                                            >  1:200683/1‑250 (MQ=255)                                                                                                                                                   gCCNAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAAGTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCTGCGTAGCGACTTTCGTTGTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACTCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTTCGTTCAGCCGATTTTGAGCATCCTCGTAAAGTTGCAAg                                                                         <  2:606699/251‑1 (MQ=255)                                                                                                                                                   gCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTTAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGTGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCaa                                                                          <  1:641237/250‑1 (MQ=255)                                                                                                                                                        gTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCt                                                                    >  1:73796/1‑251 (MQ=255)                                                                                                                                                         tGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCtg                                                                   >  1:147453/1‑251 (MQ=255)                                                                                                                                                              ggATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATgg                                                                                                                                                                                                     >  2:300270/1‑116 (MQ=255)                                                                                                                                                              ggATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATgg                                                                                                                                                                                                     <  1:300270/116‑1 (MQ=255)                                                                                                                                                               gATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCt                                                                    >  1:183808/1‑244 (MQ=255)                                                                                                                                                               gATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCt                                                                    <  2:183808/244‑1 (MQ=255)                                                                                                                                                                aTGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTTGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAAGACAAAg                                                                                                                                                                                      <  2:586105/129‑1 (MQ=255)                                                                                                                                                                aTGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAg                                                                                                                                                                                      >  1:586105/1‑129 (MQ=255)                                                                                                                                                                aTGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGa                                                             >  1:205199/1‑250 (MQ=255)                                                                                                                                                                       tctGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGc                                                     >  1:192875/1‑251 (MQ=255)                                                                                                                                                                           gcgcGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGCCGCa                                                 <  1:472657/251‑1 (MQ=255)                                                                                                                                                                            cgcgTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGCCGCAt                                                >  1:480539/1‑251 (MQ=255)                                                                                                                                                                                 ggCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATggg                                                                                                                                                                                                    <  1:137227/98‑1 (MQ=255)                                                                                                                                                                                 ggCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATggg                                                                                                                                                                                                    >  2:137227/1‑98 (MQ=255)                                                                                                                                                                                     aaTTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGCCGCATAAACGGCAt                                       <  2:73796/251‑1 (MQ=255)                                                                                                                                                                                                   tGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGCCGCATAAACGGCATCTGGAAGGtttatt                         >  1:550650/1‑251 (MQ=255)                                                                                                                                                                                                    gggCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGCCGCATAAACGGCATCTGGAAGGtttat                          <  2:200683/249‑1 (MQ=255)                                                                                                                                                                                                    gggCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCAACTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGCCGCATAAACGGCATCTGGAAGGtttatt                         <  2:419275/250‑1 (MQ=255)                                                                                                                                                                                                            gagcccTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGTGCTGGAAATACTAAGACGCCTGGATGCAGTAACATGACGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGGGCATCCTCGCAAAGGTGCAACCGGCTGGTGGGAATGGAGGCCGCATAACCGGCCTCTGGaacttttttttgcttccg                 <  2:102505/246‑17 (MQ=255)                                                                                                                                                                                                                ccTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGCCGCATAAACGGCATCTGGAAGGTTTATTTACTGCCGGAAAg            <  2:550650/251‑1 (MQ=255)                                                                                                                                                                                                                 cTGCTTTATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGCCGCATAAACGGCATCTGGAAGGTTTATTTACTGCCGGAAAgg           >  1:447270/1‑251 (MQ=255)                                                                                                                                                                                                                      ttATGCCGCGTAGCGACTTTCGTTTTATTCGCCAGCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGCCGCATAAACGGCAGCTGGAAGGTTTATTTACTGCCGGAAAggtgat       <  1:34658/250‑1 (MQ=255)                                                                                                                                                                                                                      ttATGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAACGACGCCTGGATGCAGGAACATGAGGCGGAACTTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGGGGGAATGGAAGCCGCATAAACGGCATCTGGAAGGTTTATTTACTGCCGGAAAggagatg      >  2:644363/1‑251 (MQ=255)                                                                                                                                                                                                                        aTGCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGCCGCATAAACGGCATCTGGAAGGTTTATTTACTGCCGGAAAggtgatgg     <  2:369672/250‑1 (MQ=255)                                                                                                                                                                                                                          gCCGCGTAGCGACTTTCGTTTTATTCGCCACCGCATGCTGGCACCTGAAAAAAGGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGTTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGCCGCATAAACGGCATCTGGAAGGTTTATTTACTGCCGGAAAggtgatggtga  <  1:326857/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        GCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTGGAGGATATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCGCATGAAGCCTGCTTTATGCCGCGTAGCGACTTTCGTCTTATTCGCCACCGCATGCTGGCACCTGAAAAAATGGGCTGGAAATACAAAGACGCCTGGATGCAGGAACATGAGGCGGAAATTGCACAGTTAATTCAGCATATTCATGATAAGGGGCCGGTACGTTCAGCCGATTTTGAGCATCCTCGTAAAGGTGCAAGCGGCTGGTGGGAATGGAAGCCGCATAAACGGCATCTGGAAGGTTTATTTACTGCCGGAAAGGTGATGGTGA  >  NC_000913/969447‑969914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑