Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,723,394	T→C	V425A (GTG→GCG)	ydhK →	putative transporter YdhK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,723,394	0	T	C	100.0%	193.8 / NA	54	V425A (GTG→GCG)	ydhK	putative transporter YdhK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/30);  total (24/30)

ACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGTGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCTGGCAAAAATCGTTGGCGTCGCGTTGG  >  NC_000913/1723149‑1723627                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          aCTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGAAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTc                                                                                                                                                                                                                                       >  1:478321/1‑250 (MQ=255)   tCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCaa                                                                                                                                                                                                                                     >  2:392153/1‑250 (MQ=255)      cgcGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAAttt                                                                                                                                                                                                                                 <  2:655034/249‑1 (MQ=255)        cGGACATCCGGCCTGTCTCGACATACCGATAGCGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTGGATGATGATTGGCGCATGGAGTAGTGCTGCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCATCAATTAGCTGCGTACTCTGCTCCGCCGTCGCATCACCGTTTAAGTCGTTGTCACTTCTGATGCGCCCGCTGATGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTgg                                                                                                                                                                                                                               <  2:376417/251‑1 (MQ=255)        cGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTgg                                                                                                                                                                                                                               <  1:177289/251‑1 (MQ=255)                cGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATgg                                                                                                                                                                                                                       >  2:579605/1‑251 (MQ=255)                      ggCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAg                                                                                                                                                                                                                  <  2:289962/250‑1 (MQ=255)                          cttCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAGTGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGAt                                                                                                                                                                                                                         >  2:207833/3‑239 (MQ=255)                          cttCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGAt                                                                                                                                                                                                                         <  1:207833/237‑1 (MQ=255)                          cGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGAAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCGCGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGAtt                                                                                                                                                                                                               <  2:478321/249‑1 (MQ=255)                             cATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCg                                                                                                                                                                                                           <  1:467731/250‑1 (MQ=255)                              aTACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGAt                                                                                                                                                                                                         <  1:579605/251‑1 (MQ=255)                                aCCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCt                                                                                                                                                                                                       <  1:319310/251‑1 (MQ=255)                                         gCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAAtt                                                                                                                                                                                              <  1:472197/251‑1 (MQ=255)                                          ccGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAAttt                                                                                                                                                                                             <  1:457743/251‑1 (MQ=255)                                                   tgtgGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAAt                                                                                                                                                                                               <  2:484950/240‑1 (MQ=255)                                                   tgtgGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAAt                                                                                                                                                                                               >  1:484950/1‑240 (MQ=255)                                                       gAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTctct                                                                                                                                                                                <  1:203774/251‑1 (MQ=255)                                                          cGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTc                                                                                                                                                                             >  1:360917/1‑251 (MQ=255)                                                           gggCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTcc                                                                                                                                                                            <  1:579694/251‑1 (MQ=255)                                                                    aCATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCACTTTTTACTGTTTCTCTTTCCACTGCTGGc                                                                                                                                                                   >  2:167096/1‑251 (MQ=255)                                                                             aCGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGc                                                                                                                                                           >  2:479730/1‑250 (MQ=255)                                                                                   atgatgatTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGcttct                                                                                                                                                    <  1:68098/251‑1 (MQ=255)                                                                                      atgatTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTaa                                                                                                                                                 <  2:416735/251‑1 (MQ=255)                                                                                        gatTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACGCTACTCCGCCGTCGCAGCTCCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGAGCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAAt                                                                                                                                               <  1:295740/251‑1 (MQ=255)                                                                                           tGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTACCGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGACTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTg                                                                                                                                             <  1:161635/250‑1 (MQ=255)                                                                                            ggCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGcttc                                                                                                                                                     >  1:423188/1‑241 (MQ=255)                                                                                            ggCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGcttc                                                                                                                                                     <  2:423188/241‑1 (MQ=255)                                                                                               gcATGGAGTATTGCTTCGCAATGGGATGGCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGTACCGTTTAAGTCGTTGTCCCTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGAt                                                                                                                                        <  2:542206/251‑1 (MQ=255)                                                                                                cATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTcc                                                                                                                                                                                                                    >  2:545166/1‑174 (MQ=255)                                                                                                cATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTcc                                                                                                                                                                                                                    <  1:545166/174‑1 (MQ=255)                                                                                                   ggAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGcc                                                                                                                                     <  1:580522/250‑1 (MQ=255)                                                                                                               cGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGc                                                                                                                         >  1:595860/1‑250 (MQ=255)                                                                                                                              gTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTg                                                                                                         <  2:443418/251‑1 (MQ=255)                                                                                                                               tGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGTGGCAACTg                                                                                                         >  1:424258/1‑250 (MQ=255)                                                                                                                                  cAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTg                                                                                                     <  1:371569/251‑1 (MQ=255)                                                                                                                                   aaTGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGt                                                                                                    >  1:376775/1‑251 (MQ=255)                                                                                                                                    aTGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGGCGCAGCACCGGTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGtt                                                                                                   <  2:582179/251‑1 (MQ=255)                                                                                                                                     tGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGttt                                                                                                  >  2:218308/1‑251 (MQ=255)                                                                                                                                              gatGGCAGCAATTAGCTGCGTACTCTACTCCACCGTCGGAGCGCCGTTTAAGTCGGTGTCACTTCTGATGCGCACGCTGGTGTTACCTTCGCTATTCAGCTTTGCGGTCAATTTTGGGTTGATGGTCCCGATTAGCGATCTGTGGCAATTTTTGCGGTTTCTCGTTCCACTGCTGGCGACACTGCAGCTTCTTAAATTGCAGATGCGAAAATTTGCCGCATTGTGGCGGCGACTGATTGTTTTTATgcgtc                                                                                         <  2:337733/249‑5 (MQ=255)                                                                                                                                                 ggCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCtt                                                                                      >  1:196411/1‑251 (MQ=255)                                                                                                                                                 ggCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCt                                                                                       <  2:500295/250‑1 (MQ=255)                                                                                                                                                       aaTTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCg                                                                                >  1:330080/1‑251 (MQ=255)                                                                                                                                                          tAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCaaaa                                                                                                                                 <  1:184369/199‑1 (MQ=255)                                                                                                                                                          tAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCaaaa                                                                                                                                 >  2:184369/1‑199 (MQ=255)                                                                                                                                                           aGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGt                                                                            >  2:368495/1‑251 (MQ=255)                                                                                                                                                                          tcCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCggg                                                             >  2:349067/1‑250 (MQ=255)                                                                                                                                                                                                    gTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACg                                   >  2:531990/1‑251 (MQ=255)                                                                                                                                                                                                           cTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCt                            >  1:261711/1‑251 (MQ=255)                                                                                                                                                                                                            ttCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTCAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCTg                           >  2:535178/1‑251 (MQ=255)                                                                                                                                                                                                                      cACGCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCGTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCTGGCAAAAATCg                 <  1:80091/251‑1 (MQ=255)                                                                                                                                                                                                                         gCTGGTGTTACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCTGGCAAAAATCGTTg              >  1:264598/1‑251 (MQ=255)                                                                                                                                                                                                                                ttACTTTCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCTGGCAAAAATCGTTGGCGTcgc       >  1:311206/1‑251 (MQ=255)                                                                                                                                                                                                                                      tCGCTATTCAGCTTTGCGGTCAAATTTGGTCTGATGGTCCATATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCTGGCAAAAATCGTTGTCGTCGCGTTgg  <  2:253999/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          ACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCCGTCGCAGCACCGTTTAAGTCGTTGTCACTTCTGATGCGCACGCTGGTGTTACTTTCGCTATTCAGCTTTGTGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCTGGCAAAAATCGTTGGCGTCGCGTTGG  >  NC_000913/1723149‑1723627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑