Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,047,801	C→T	S414N (AGC→AAC)	gcvP ←	glycine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,047,801	0	C	T	96.9%	331.4 / NA	99	S414N (AGC→AAC)	gcvP	glycine decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (49/46);  minor base G (0/3);  total (49/50)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGCTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACA  >  NC_000913/3047563‑3048039                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               gTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTgg                                                                                                                                                                                                                                    >  1:450055/1‑250 (MQ=255)  tGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGCTAGGTGAGGATTTCGTCGTCGCGCAGAATCGCAGGCTGGACAGAGCGGCTGACGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGCGGTTATCGCCCAGCAGCACGTTGAAAAGCTGAATTACGATTTCACGCGTGGTTGTTGCATCAAGGGTGATCACAACAGCGTTCAGAATATCGTTAAGCAGGTTGAt                                                                                                                                                                                                                                   >  1:66802/1‑251 (MQ=255)       ataGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAg                                                                                                                                                                                                                              <  1:353243/251‑1 (MQ=255)         aGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGTTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGc                                                                                                                                                                                                                             <  2:92992/250‑1 (MQ=255)         aGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGcc                                                                                                                                                                                                                            >  1:359886/1‑251 (MQ=255)                catTTCGGTTTCGCGGTGGTAGCGATTAAACGCCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGGGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTTGTTATCGCCCAGCAGCACGTGGAAAAGGTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTTACCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCgg                                                                                                                                                                                                                     <  1:50861/251‑1 (MQ=255)                         ttCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTc                                                                                                                                                                                                             <  1:505901/250‑1 (MQ=255)                         ttCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGCTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTc                                                                                                                                                                                                             <  1:364677/250‑1 (MQ=255)                            gCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGt                                                                                                                                                                                                          >  1:126353/1‑250 (MQ=255)                               gtcgTAGCGATTGATCACCGGAGGGGTGAGGATTTCGTGGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGACCCACGTCTCTGTCCAGCGTGACGCTGTCCACGCCGAGGTTATAGCCCACAAGCACGTTGAACAGTAGCATTACGTTTTCACGCCTGGTTGTTTCATCAAGGGAGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTCCGc                                                                                                                                                                                                      >  2:293933/4‑251 (MQ=255)                                          tAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTc                                                                                                                                                                                            <  1:496025/250‑1 (MQ=255)                                           aaaCACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCgg                                                                                                                                                                                          <  2:359886/251‑1 (MQ=255)                                             acacCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGcc                                                                                                                                                                                        >  2:165802/1‑251 (MQ=255)                                               cccGGATGGGTGAGGATTTCGTCGGCGCGCAGCATCGCAGGCTGGATAGAGCGGCGGTCGTGAGACACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCGCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATAGCGTTACGCAGGTTGAGTACAGCCGCGTCGGCACGCGGCAGTACGCCTGCGTTGTCGGCCAc                                                                                                                                                                                      <  2:473730/250‑1 (MQ=255)                                                       ggTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCcacac                                                                                                                                                                              >  1:592333/1‑251 (MQ=255)                                                        gTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCcacac                                                                                                                                                                              >  1:81144/1‑250 (MQ=255)                                                          gAGGATTTCGTCGCCGTGCAGCATCGGAGGCTGGATAGAGCGACTGTCGTGAGCCACGTACTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAATCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCcacacaca                                                                                                                                                                           <  1:333382/251‑1 (MQ=255)                                                          gAGGATCTAGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGACGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCcacacac                                                                                                                                                                            >  2:62860/1‑250 (MQ=255)                                                           gggNTTTCGTAGTCGGGCTGCATCGCAGGGTGGGTAGGGCTGCTGTCGTGTGCCTCGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCTCGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCGAGGGTGATCCCATCCGCGTTCAGTATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCTTCAGTTCGCCCGCTTTGTCGGGCACTTCCACACACaa                                                                                                                                                                          <  2:599617/250‑1 (MQ=255)                                                                  cgtcgtcgCGCAGCATCGCAGGCTGGATAGAGCGGCTGTTGTGAGCCACGTCGTTGTCCAGCGTGTCGATGTCCAGGCCCTGGTTATCGCCCAGCAGCACGTTGAGAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATGAAGGGTGATCCCAACCGCGTTCAGAATATCGGTACGCAGGTTGATTTCAGCTGCTTCGGCACGTGTCAGTACGTCCGCTTTGTCGGCCACTTCCGCACACAAGGTGTc                                                                                                                                                                    <  2:634032/250‑1 (MQ=255)                                                                  cgtcgtcgCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAGAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCg                                                                                                                                                                   <  2:125671/251‑1 (MQ=255)                                                                   gtcgtcgCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGGCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTTAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGGCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCg                                                                                                                                                                   >  1:304928/1‑250 (MQ=255)                                                                   gtcgacgCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGACGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACCAGGTGTCg                                                                                                                                                                   >  2:363539/1‑250 (MQ=255)                                                                    tcgtcgCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACCCACAAGGTGTCGa                                                                                                                                                                  >  2:126187/1‑250 (MQ=255)                                                                      gtcgCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAAt                                                                                                                                                               >  2:509796/1‑251 (MQ=255)                                                                        cgcgCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGGTTTCACGCGTGGTTGTTTCATCAAGGTTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCATGCGTCAGTACGCCCGGTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAg                                                                                                                                                             <  2:629307/251‑1 (MQ=255)                                                                          cgcAGCATCTCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTTATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCTCTTCCACACACAAGGTGTCGAAATa                                                                                                                                                              <  1:352253/248‑1 (MQ=255)                                                                          cgcAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAGAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAACTAGTg                                                                                                                                                           >  1:552742/1‑251 (MQ=255)                                                                              gcaTCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCCCACACACGGTGTCGAAATAGTgcgc                                                                                                                                                        >  1:587867/1‑250 (MQ=255)                                                                                aTCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGACGTGGTTATCGCCGAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTAAGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATg                                                                                                                                                     >  1:638872/1‑251 (MQ=255)                                                                                    cAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGgcgc                                                                                                                                                 >  1:414933/1‑251 (MQ=255)                                                                                     aGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCCTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCa                                                                                                                                                <  1:245207/251‑1 (MQ=255)                                                                                          ggATAGAGCGGCTTTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCTTTACGCAGGTTGATTTCAGCCGCTTTGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACTCAAGGTGTCGAAATAGTGCGCATGGCGCAGtt                                                                                                                                             <  1:635736/249‑1 (MQ=255)                                                                                            aTAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTGCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAg                                                                                                                                         <  2:260781/251‑1 (MQ=255)                                                                                              agagCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGa                                                                                                                                        <  1:548444/250‑1 (MQ=255)                                                                                                    gCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCttttt                                                                                                                                 >  1:589355/1‑251 (MQ=255)                                                                                                     cTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTa                                                                                                                                                                                                         <  2:425481/178‑1 (MQ=255)                                                                                                     cTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTa                                                                                                                                                                                                         >  1:425481/1‑178 (MQ=255)                                                                                                         cGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGc                                                                                                                            <  2:457156/251‑1 (MQ=255)                                                                                                           tGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGc                                                                                                                            >  1:624404/1‑249 (MQ=255)                                                                                                           tGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGc                                                                                                                            >  1:241728/1‑249 (MQ=255)                                                                                                               ccACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGccc                                                                                                                      <  2:573078/251‑1 (MQ=255)                                                                                                                  cGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCccgcc                                                                                                                   >  1:495334/1‑251 (MQ=255)                                                                                                                    tCTTTGTCCAGCGTGTCTATGTCCAGGCCTTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTTCGTTTTCGCGCGTGGTTGTTTCATCATGTGTGATCCCAACCTCGTTCACAATATCGTTACGCGGGTTGATTTCATCCTCTTCGGCGCGCGTCACTACGCCCGGTTTGTCGGCCTCTTCCACACACAAGGGGTCGAAATAGTGCGCATGGCGAAGTTTCAGACCTTTTTTTTGCAGGCccgccg                                                                                                                  <  2:631521/250‑1 (MQ=255)                                                                                                                    tCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCcgccgc                                                                                                                 >  1:210069/1‑251 (MQ=255)                                                                                                                       ttGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCCCTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCa                                                                                                               <  2:181266/250‑1 (MQ=255)                                                                                                                             aGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGCGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATc                                                                                                        >  1:163185/1‑251 (MQ=255)                                                                                                                              gCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCg                                                                                                       <  2:383821/251‑1 (MQ=255)                                                                                                                               ggggtCGATGTCCAGGCCGTGGGTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCgg                                                                                                      <  2:532918/248‑1 (MQ=255)                                                                                                                                gtgccgatttgcatgccgtcgtatgcGCCCAGCTGCACGTTAAAAAGCTGCATTACGTTTGCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGGTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCTCTTTGTCTGCCACTTCCACTCACAAGTTGTCGAAATAGGGCGGATGGCGCAGTTGCAGACCTTTTTGTTGCCGGGCCGCCGCCAGGATAGCGGt                                                                                                     <  2:80006/226‑1 (MQ=255)                                                                                                                                     gATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGAc                                                                                                <  2:444992/251‑1 (MQ=255)                                                                                                                                      aTGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACg                                                                                               >  2:301053/1‑251 (MQ=255)                                                                                                                                         tCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTg                                                                                            >  1:6713/1‑251 (MQ=255)                                                                                                                                          ccAGGCCGTGTTTATCGCTCAGCAGAAAGTTGAAAAGCGGCAGTACGTTTGCACGCTTGGTTGTTTTATCAAGGGTGATCGCAACCGCGTTCAGAATATCTTTACGCAGGGTGATCTCAGCCGCTTCGGCACGCGTCAGTACGCCCTCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATCTCTGTCCGACGGTGa                                                                                           <  2:308430/251‑1 (MQ=255)                                                                                                                                             ggCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGTCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTTCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGGCAGACGGTGAATg                                                                                        <  2:447598/251‑1 (MQ=255)                                                                                                                                              gCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCTCCAGGATATCGGTCAGACGGTGAATGc                                                                                       <  1:570642/251‑1 (MQ=255)                                                                                                                                               ccGTGGTTAACGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCg                                                                                      <  1:174928/251‑1 (MQ=255)                                                                                                                                                cGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACTTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCgg                                                                                     <  1:543139/251‑1 (MQ=255)                                                                                                                                                cGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCgg                                                                                     <  2:70006/251‑1 (MQ=255)                                                                                                                                                 gTGGTTATCGCCCAGCAGCACGTTGAAACCCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGAAATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTGTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGt                                                                                    >  2:189496/1‑251 (MQ=255)                                                                                                                                                      tATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCg                                                                               >  1:597294/1‑251 (MQ=255)                                                                                                                                                             cagcagCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTAAGACGGTGAATGCGGTTAGCGATACGtt                                                                        >  2:335704/1‑251 (MQ=255)                                                                                                                                                                 gggccGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATGTCAGCCGCTGCGGCCCGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAg                                                                    <  2:446677/247‑1 (MQ=255)                                                                                                                                                                   cccGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCACATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGc                                                                  <  2:9992/249‑1 (MQ=255)                                                                                                                                                                   caCGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGc                                                                  >  1:635449/1‑251 (MQ=255)                                                                                                                                                                    aCGNTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCACCCTCGTTCAGAATATCGGTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGcc                                                                 <  2:589355/251‑1 (MQ=255)                                                                                                                                                                      gTTGAAACGCTGCATTACGTTTTCACGCATGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCCGGTTGATTTCAGCCGCTTCGGAACGCGTCAGTACGCCCGCTTTGTCGGCCAATTCCACACACAAATTGTCGAAATAGAGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCGCGCTGTCAGGATATCGGTCAGAAGGTGAATGCGGTTAGCGATCCGTGTCCGGCCa                                                                >  2:490444/1‑250 (MQ=255)                                                                                                                                                                       ttGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACAGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGt                                                                                                                                                            >  1:621243/1‑157 (MQ=255)                                                                                                                                                                       ttGAAAAGCTGCATGACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGt                                                                                                                                                            <  2:621243/157‑1 (MQ=255)                                                                                                                                                                          aaaaGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCgg                                                           >  2:317189/1‑251 (MQ=255)                                                                                                                                                                           aaaGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCggg                                                          >  1:560295/1‑251 (MQ=255)                                                                                                                                                                           aaaGCTGCATTACGTTTTCAAGCGTGGTTGTTTCATCAACGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTAGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGACGTTTCAGACCTTTTTGTTGCAGGCCCTCCGCCAGGATATCGGTCAGACGGGGAATGCGGTTAGCGCTACGTTTCATGCCAACCggg                                                          >  2:646082/1‑251 (MQ=255)                                                                                                                                                                               cTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGt                                                      >  1:405536/1‑251 (MQ=255)                                                                                                                                                                                 ggattccattttctcgtggggTTGTTTAAGCAAGGGTGGTCCCAACCGCTTTCAGAATATCGTTCCGCCGCTTGATTGCATCCGCTGCGGCACGCGGCAGTACGCCTGCTTTGGCGGCCACCGCCACACACAATGTGTCGAAATAGTGCGCATGGCGCAGTTTCATACCTTTTGTTTGCAGTCCCGCCGCCAAGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCTACCGGGCCTTGa                                                    <  2:134280/232‑1 (MQ=255)                                                                                                                                                                                  cATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGGGACGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGGGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCAGTGa                                                    >  2:501246/1‑250 (MQ=255)                                                                                                                                                                                    ttACGTTTTCACGCGTGGTTGTTTCATCCAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATa                                                  >  2:324412/1‑250 (MQ=255)                                                                                                                                                                                        gTTTTCACGCCTGGCTATTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATAGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGAAAGCTTTGTCGGCCACTTCCACACACCAGGTGTCGAAATAGTGCGCAAGGCGCAGGTTGAGACCTTTTTGATGCAGGCCCGCCGCCAGGATATCGAGCAGACGCTGCAAGCGGTTAGCGATACGTGTCAGGCCAAACGGGCCGGGATAAACgg                                             >  2:252476/1‑251 (MQ=255)                                                                                                                                                                                              aCGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATAc                                        >  1:82048/1‑250 (MQ=255)                                                                                                                                                                                              aCGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACa                                       <  2:414933/251‑1 (MQ=255)                                                                                                                                                                                                 cgTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGc                                    >  1:369228/1‑251 (MQ=255)                                                                                                                                                                                                  gTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCt                                   <  1:479095/251‑1 (MQ=255)                                                                                                                                                                                                    ggTTGTTTCATCAAGGGTGCTCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTCCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTgg                                 <  2:126353/251‑1 (MQ=255)                                                                                                                                                                                                        gttTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGa                              <  2:178537/250‑1 (MQ=255)                                                                                                                                                                                                        gttTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGAt                             <  2:81144/251‑1 (MQ=255)                                                                                                                                                                                                         gttCATCAAGGGTGATCCAACCCGCGTTCAGAATATCGTTACGCAGGTTGATTCCAGCCGCTCCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATGGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGAt                             <  2:456027/249‑1 (MQ=255)                                                                                                                                                                                                          ttCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATgg                           >  2:431213/1‑250 (MQ=255)                                                                                                                                                                                                            catcaAGGGTGATCCCACCCGCGTTCAGAATATCGTTACGCAGGTTGAGTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGGTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGtt                          <  2:334661/250‑1 (MQ=255)                                                                                                                                                                                                              tcaAGGGGGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGAATTCAGCCGCATCGGCACGCGACAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTAGAAATAGTGCGCATGGCGCAGATTAAGACCTTTTTGTTGCAGGCCCGCAGCCAGGATATCGGTAAGACGGAGAATGAGGTTAGCGATACGTTTCAGGCCAACCGGGACGGGATAAACGGCATACAGGCTGGCGATGGTTGc                       >  2:482764/1‑251 (MQ=255)                                                                                                                                                                                                                 aGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGcc                      >  1:360042/1‑249 (MQ=255)                                                                                                                                                                                                                 aGGGTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCcag                    <  1:208572/251‑1 (MQ=255)                                                                                                                                                                                                                  gggTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCcagc                   >  2:227127/1‑251 (MQ=255)                                                                                                                                                                                                                    gTGATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCcagcag                 <  1:36110/251‑1 (MQ=255)                                                                                                                                                                                                                      gATCCCAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATACACGGCATACAGGCTGGCGATGCTTGCCAGCAGt                >  1:565464/1‑250 (MQ=255)                                                                                                                                                                                                                           cAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGCCCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTgg          <  2:324512/251‑1 (MQ=255)                                                                                                                                                                                                                           cAACCGCGTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTgg          >  1:518628/1‑251 (MQ=255)                                                                                                                                                                                                                                  gTTCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTAc   <  1:301053/251‑1 (MQ=255)                                                                                                                                                                                                                                    tCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAgg                                                                                                                         <  2:615992/131‑1 (MQ=255)                                                                                                                                                                                                                                    tCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAgg                                                                                                                         >  1:615992/1‑131 (MQ=255)                                                                                                                                                                                                                                    tCAGAATATCGTTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACa  >  2:532377/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               GTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGCTACGCAGGTTGATTTCAGCCGCTTCGGCACGCGTCAGTACGCCCGCTTTGTCGGCCACTTCCACACACAAGGTGTCGAAATAGTGCGCATGGCGCAGTTTCAGACCTTTTTGTTGCAGGCCCGCCGCCAGGATATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACA  >  NC_000913/3047563‑3048039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑