Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,134,882	C→T	P52S (CCG→TCG)	flgG →	flagellar basal‑body rod protein FlgG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,134,882	0	C	T	100.0%	160.4 / NA	47	P52S (CCG→TCG)	flgG	flagellar basal‑body rod protein FlgG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/18);  total (29/18)

GCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCTCTTTCCAGGTGGATCAGAACGGGCAGCTGGTGACGGCTGGTGGTT  >  NC_000913/1134640‑1135128                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       gCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCTCTTTTTCACTAATCCACTACAGGACATTTTATGATCAGGTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCGACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGTGCGCGGTGTTTGAATATCTGCTTTATCAACCCATTCGCCAGTCGGGGGca                                                                                                                                                                                                                                                <  2:219480/251‑1 (MQ=255)   gAGCACTTGTAGGCAGGATAAGGCGTTTACGCAGCATCCGGCAAGAAGAAATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGCTCGCCAAAACGGGACATGACGACAAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGcac                                                                                                                                                                                                                                               >  2:95974/1‑250 (MQ=255)          tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACTCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCtt                                                                                                                                                                                                                                         <  1:626125/249‑1 (MQ=255)           gtagaccagATAAGGCGATTACGCCGCATCCGGCACGAAGACATATGAACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGCGACTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCCTTCGCCAGTCGGGGGCCCAGTCTTCCg                                                                                                                                                                                                                                      >  2:377858/9‑251 (MQ=255)                          cGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTAc                                                                                                                                                                                                                       <  2:478415/251‑1 (MQ=255)                           gtgtACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAGCCACCTTAcc                                                                                                                                                                                                                      <  2:624752/248‑1 (MQ=255)                                 gccgcATCCGGCAAGAAGGCATATTCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGAGCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCgg                                                                                                                                                                                                                <  2:526902/251‑1 (MQ=255)                                     cATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCggctt                                                                                                                                                                                                             >  2:415342/1‑247 (MQ=255)                                                                      aaTCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACt                                                                                                                                                                           <  1:638896/251‑1 (MQ=255)                                                                               cAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCtt                                                                                                                                                                   >  2:504217/1‑250 (MQ=255)                                                                                                       tATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGcaca                                                                                                                                                                                                                                              >  2:96273/1‑151 (MQ=255)                                                                                                       tATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGcaca                                                                                                                                                                                                                                              <  1:96273/151‑1 (MQ=255)                                                                                                       tATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGc                                                                                                                                            >  1:389168/1‑249 (MQ=255)                                                                                                             tCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACnaac                                                                                                                                    >  2:601562/1‑251 (MQ=255)                                                                                                              cGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACcaac                                                                                                                                    <  1:504217/250‑1 (MQ=255)                                                                                                                ccAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCaacaac                                                                                                                                 <  2:389168/251‑1 (MQ=255)                                                                                                                       gggCCTTGACGCCCAGCAAACCACTATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTAc                                                                                                                                                                                                                       >  2:328190/1‑158 (MQ=255)                                                                                                                       gggCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTAc                                                                                                                                                                                                                       <  1:328190/158‑1 (MQ=255)                                                                                                                        ggCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCaa                                                                                                                            >  1:54174/1‑248 (MQ=255)                                                                                                                          ccTTGACGCCCAGCAAACCAATAAGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGCTCTGCTTTATCAAACCATTCGCCCGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAGACCTGTCGCAGCCCAACAACAGCAAAGATg                                                                                                                       >  1:497512/1‑251 (MQ=255)                                                                                                                                cGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGa                                                                                                                 <  2:289101/251‑1 (MQ=255)                                                                                                                                     aGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTaa                                                                                                             >  2:581170/1‑250 (MQ=255)                                                                                                                                                      gTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCa                                                                                           <  1:514067/251‑1 (MQ=255)                                                                                                                                                       tCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATATGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGATCAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCATACCAACAACAGCAAAGATGTCGCACTTACAGGGCAGGGATCTTTcc                                                                                            >  2:582429/1‑249 (MQ=255)                                                                                                                                                         aTTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCGCGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGt                                                                                        <  2:120674/251‑1 (MQ=255)                                                                                                                                                                acaacCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTg                                                                                 >  2:98240/1‑251 (MQ=255)                                                                                                                                                                  aacCTGGCAACCGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGATGTTTGAAGCTCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTACGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAAAAACAGCAAAGATGCCGCGATTAAAGGGCAGGGCTTTTTCCAGGAGATGTTGcc                                                                               >  2:360562/1‑251 (MQ=255)                                                                                                                                                                   acCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGCCTTCCGAACAAACCACCTTCCCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGAGGTCGCGATTAAAGGGCAGGGCTGTTTCTAGGTGATGTTGCCa                                                                              <  2:87632/251‑1 (MQ=255)                                                                                                                                                                      tGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGcc                                                                               >  1:211697/1‑247 (MQ=255)                                                                                                                                                                      tGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGcc                                                                               >  1:38456/1‑247 (MQ=255)                                                                                                                                                                           aaCGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTtc                                                                      >  1:88353/1‑251 (MQ=255)                                                                                                                                                                                    aCTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCt                                                              >  1:411737/1‑250 (MQ=255)                                                                                                                                                                                          ggTTGTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGGACGCCCGGTCGCCACTGAACGCGTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTGTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCg                                                       <  2:54174/251‑1 (MQ=255)                                                                                                                                                                                           gTTTTAAGCGTCAGCGCGCTGTGTTTGAAGATCTGGTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCGCCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCGCTGAACGCTTACGCAGCCAGGGAAACCTGTCGCAGACCAGCAACAGCAAAGATGTCGCGATTAAATGGCAGTGGTGTTTCCAGGTTATGTTGCCAGATGGTTCATCAGCCTATACCCGt                                                      <  1:40706/251‑1 (MQ=255)                                                                                                                                                                                                aaGCGTCAGCGCGCTGTTCTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCATCGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGCTATTTTGCCAGATGGTTCCTCAGCCTATCCCCGTGNCgg                                                 >  2:609033/1‑251 (MQ=255)                                                                                                                                                                                                aaGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACgg                                                 >  1:548570/1‑251 (MQ=255)                                                                                                                                                                                                      cAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCTCttt                                           >  1:618433/1‑251 (MQ=255)                                                                                                                                                                                                      cAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCCCttt                                           >  1:169807/1‑251 (MQ=255)                                                                                                                                                                                                            gcgGTGCTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGAACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGACGCGATTAAAGGGCAGGGCTTTTTCCAGGGGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCTCTTTCCAggg                                     >  1:413833/1‑250 (MQ=255)                                                                                                                                                                                                             cgGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCTCTTTCCAGGTg                                    >  2:131868/1‑251 (MQ=255)                                                                                                                                                                                                              ggTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCTCTTTCCAGGTgg                                   >  2:80215/1‑251 (MQ=255)                                                                                                                                                                                                                 gtTTGAAGATCTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCTCTTTCCAGGTGGATc                                >  1:261285/1‑251 (MQ=255)                                                                                                                                                                                                                           cTGCTTTATCAAACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCTCTTTCCAGGTGGATCAGAACGGGc                       >  2:347972/1‑250 (MQ=255)                                                                                                                                                                                                                              cTTTATCACACCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCTCTTTCCAGGTGGATCAGAACGGGCAGCt                   >  2:500279/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaaCCATTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCTCTTTCCAGGTGGATCAGAACGGGCAGCTGGTGACg            >  1:616992/1‑251 (MQ=255)                                                                                                                                                                                                                                          aTTCGCCAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCTCTTTCCAGGTGGATCAGAACGGGCAGCTGGTGACGGCtgg       <  2:480355/251‑1 (MQ=255)                                                                                                                                                                                                                                               ccAGTCGGGGGCACAGTCTTCCGAACAAACCACCTTCCCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCTCTTTCCAGGTGGATCAGAACGGGCAGTTGGTGACGGCTGGTGGtt  <  1:563151/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       GCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACGGTTTTAAGCGTCAGCGCGCGGTGTTTGAAGATCTGCTTTATCAAACCATTCGCCAGCCGGGGGCACAGTCTTCCGAACAAACCACCTTACCCTCCGGATTACAAATCGGCACGGGGGTACGCCCGGTCGCCACTGAACGCTTACACAGCCAGGGAAACCTGTCGCAGACCAACAACAGCAAAGATGTCGCGATTAAAGGGCAGGGCTTTTTCCAGGTGATGTTGCCAGATGGTTCATCAGCCTATACCCGTGACGGCTCTTTCCAGGTGGATCAGAACGGGCAGCTGGTGACGGCTGGTGGTT  >  NC_000913/1134640‑1135128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑