Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,100,526	G→A	A68V (GCG→GTG)	ansB ←	L‑asparaginase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,100,526	0	G	A	100.0%	182.7 / NA	61	A68V (GCG→GTG)	ansB	L‑asparaginase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/35);  total (26/35)

GTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCAT  >  NC_000913/3100279‑3100760                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           gTTGAATGGACCGTCTGCGCTCATAGACGTGGACTGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACaa                                                                                                                                                                                                                                         <  2:319866/251‑1 (MQ=255)   tGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAAtt                                                                                                                                                                                                                                       >  2:107349/1‑250 (MQ=255)    gAATGGTCCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATg                                                                                                                                                                                                                                       <  1:467356/250‑1 (MQ=255)      aTGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTTACTACCTGCTCGCCTTTAACGTTCACAATGTCt                                                                                                                                                                                                                                    >  2:532394/1‑251 (MQ=255)             gTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGtt                                                                                                                                                                                                                             >  2:292836/1‑251 (MQ=255)                 gcgcTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTGCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTGCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGc                                                                                                                                                                                                                           <  1:273702/249‑1 (MQ=255)                   gcTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGc                                                                                                                                                                                                                        >  1:531846/1‑250 (MQ=255)                    cTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCAc                                                                                                                                                                                                                      >  1:620343/1‑251 (MQ=255)                          gACGTGGACGGACGAATTGCGCCGACCATCACCGCCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTTTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCAtt                                                                                                                                                                                                                <  2:559210/251‑1 (MQ=255)                          gACGGGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCAtt                                                                                                                                                                                                                <  2:238817/251‑1 (MQ=255)                                            gcgcCGACCATCACCACCGGTTTGTCGCATTGCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGGTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGcc                                                                                                                                                                                              <  2:503474/251‑1 (MQ=255)                                               ccGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGTTCGAGGAAGTAAGCAGTTTCTTACATCGTGTCGGTACCGTGGGAAATGACGCAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGTTATTCACAACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCCTTAACCAGATTTTCTACGCCAAc                                                                                                                                                                                           >  2:408296/1‑251 (MQ=255)                                                   ccATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTa                                                                                                                                                                                       <  1:107349/251‑1 (MQ=255)                                                             cGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTgtgt                                                                                                                                                                             <  1:363731/251‑1 (MQ=255)                                                                 ttGTCGCATGTCACCGTCAGGTCGAGGACGTAAGCAGTTTCTTCCATCGTTACGGTACCGTGGGTAATGACGAAGAAGTCGGGCTTCTCGCAGTCGGTGTTAATTTTTTACGCAAGTGACAGCCAGACATTAACGGTACTGTCCTGGGAGCCGATATTCACTACCTGCTCTCCTTTAACGTTCAACATGTCTTTTAGTGGAAGCACAGTATGAAACAGATTTTTTCCGCCACCTCTACCCTCTGTGGAGtt                                                                                                                                                                         >  2:460583/1‑251 (MQ=255)                                                                     cGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGa                                                                                                                                                                      <  1:430064/250‑1 (MQ=255)                                                                      gCATTCCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCGCTACCTGCTCGCGTTTAACGTTCACAATGTCTTTTAGTTGCGGCGCCGCTTTAACCAGATTGTCTACGCCACCTTTACCCACTGTGTAGTTAGAtt                                                                                                                                                                    <  2:557228/251‑1 (MQ=255)                                                                       cATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGGCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGAttt                                                                                                                                                                   <  1:316511/251‑1 (MQ=255)                                                                                gTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCggg                                                                                                                                                          >  2:93509/1‑250 (MQ=255)                                                                                       cGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTcacc                                                                                                                                                    >  1:374287/1‑250 (MQ=255)                                                                                           gaatgaaGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGGAGCTGTCGGTCGTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCGGCCAGACAGTATCGTTCAAGTCCTGGGAGCCGATATTCACAGCCTGCTCGGCTTTAACGTTCACGATGTCTTTTAATTGCCGCACCGCATTAACCAGATTTTCTACGCCAACTGTCCCCACTGTGTAGTTAGATTTGGTTGCGGAGTctccactac                                                                                                                                               <  2:448968/246‑4 (MQ=255)                                                                                                           tcCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCccg                                                                                                                                 <  1:275628/249‑1 (MQ=255)                                                                                                           tcCATCGGGTCGGTACCGTGGGTAATGTCGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCGGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCTTCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCCCCGGCAATGGTCccg                                                                                                                                 <  2:154284/249‑1 (MQ=255)                                                                                                                cGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCTTTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTg                                                                                                                          >  2:218971/1‑251 (MQ=255)                                                                                                                cGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTg                                                                                                                          <  2:374287/251‑1 (MQ=255)                                                                                                                  tgtCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTNGCt                                                                                                                        >  2:602908/1‑251 (MQ=255)                                                                                                                            gTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATgggg                                                                                                               >  2:397782/1‑248 (MQ=255)                                                                                                                              gggTAATGACGAAGCCGTCGGTCTTATCGCAGTTGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGTGCCGATATTCACTACCTTCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTACCCAGATTTTCTACGCCAACTTTACCCTCTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCCCCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGata                                                                                                            <  1:661382/251‑1 (MQ=255)                                                                                                                                gTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTCCCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACGGTGTAGTTAGATTTGGTTGCGGAGTCCCCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATAtt                                                                                                          <  1:135906/251‑1 (MQ=255)                                                                                                                                   aTGACGAGGCCGCCGGGTTTATCGCAGTCGGTTTTAATTTTTTTCGCCATTGTCGGCCAGACATTATCTTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTCCGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCCCCCCCGCCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTggg                                                                                                       <  2:221026/251‑1 (MQ=255)                                                                                                                                     gACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTTCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGt                                                                                                      <  1:57885/250‑1 (MQ=255)                                                                                                                                     gACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGt                                                                                                      <  1:559531/250‑1 (MQ=255)                                                                                                                                      aCGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTa                                                                                                     >  1:393571/1‑250 (MQ=255)                                                                                                                                        gAAGCCGTCGGTCTTATCGCAGTCGGTTTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCCCCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATg                                                                                                  <  1:215899/251‑1 (MQ=255)                                                                                                                                              gTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAAtg                                                                                            <  1:238662/251‑1 (MQ=255)                                                                                                                                                cgttCTTAACGCAGTCGGTGTTAATTTTTTTCCCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGCGCTGATATTCACTACCTGCTCTCCTTTAACGTTAACAATTTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTTCCCACTGGTTAGTTAGATTTGGTTGCGGATTCACCACCACCGGCAATGGACCCGCCGGTTGCTAAAATGGTGATATTGTGTAATGCCAAtgct                                                                                          <  1:611756/248‑1 (MQ=255)                                                                                                                                                 ggTCTTATCGCGGTAGGTTTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGCTTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGCCACTGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCActgctg                                                                                         >  2:63384/1‑251 (MQ=255)                                                                                                                                                 ggTCTTATCGCAGTCGTTGTTAATTTTTTTCGCCAGTGTCAGCCAGCCATTATCGTTCATTTCCTGGGAGCCGATATTCACTGCCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCCCTGTGTAGTTAGATTGGGTTGCGGAGTCCCCACCACCGGTAGTGGTCCCGCCGGTTGCTAAAATGGTGTTATTGGGTAATGCCAAtgctg                                                                                         <  1:51332/251‑1 (MQ=255)                                                                                                                                                      tATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGcaccc                                                                                    >  1:535186/1‑250 (MQ=255)                                                                                                                                                            aGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTaaa                                                                               >  2:357476/1‑250 (MQ=255)                                                                                                                                                                ggTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGCGATATTGGGTAATGCCAATGCTGCACCACTAAAAccc                                                                           >  1:626003/1‑250 (MQ=255)                                                                                                                                                                      aaTTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGGTATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACTCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGGGATATTGGGTAATCCCAATGCTGCACCACTAAAACCCATAAcc                                                                     >  2:321630/1‑250 (MQ=255)                                                                                                                                                                       aTTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAAcc                                                                     >  1:527828/1‑249 (MQ=255)                                                                                                                                                                         ttttttCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCCCCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGt                                                                  <  1:275835/250‑1 (MQ=255)                                                                                                                                                                           ttttCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGc                                                                <  1:167661/250‑1 (MQ=255)                                                                                                                                                                            tttCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCg                                                               <  1:272626/250‑1 (MQ=255)                                                                                                                                                                                      cccctacccgtcgctcttccgatctCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACGGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGGCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGt                                                    <  2:284543/227‑1 (MQ=255)                                                                                                                                                                                                cATTATCGTTCATGTCCTGGGAGCCGATATTCACTCCCTGCTCTCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTCCGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTGGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTTTTTTTGaaa                                          <  1:142232/251‑1 (MQ=255)                                                                                                                                                                                                           aTGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTc                               >  2:181694/1‑251 (MQ=255)                                                                                                                                                                                                               ccTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAACATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGt                                                    >  1:284543/1‑226 (MQ=255)                                                                                                                                                                                                                  gggAGCCTATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTCCCCACTGTGTAGTTAGATTTGGTTGCGGAGTCCCCACCACCGGCAATGGTTCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATtcc                          <  2:75600/249‑1 (MQ=255)                                                                                                                                                                                                                   ggAGCCGTTATTCACTACCTGCCCGCCTTTAACGTTCACAGTGGCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTCCCCCCTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATtcctcc                       <  2:542619/251‑1 (MQ=255)                                                                                                                                                                                                                   ggAGCCGATATTCACTCCCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATtcctcc                       <  1:611056/251‑1 (MQ=255)                                                                                                                                                                                                                   ggAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATtcctcc                       >  1:12310/1‑251 (MQ=255)                                                                                                                                                                                                                   ggAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAATGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATtcctcc                       >  2:510258/1‑251 (MQ=255)                                                                                                                                                                                                                            atTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTg              >  1:503687/1‑251 (MQ=255)                                                                                                                                                                                                                            atTCACTACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGGAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTg              <  2:276120/251‑1 (MQ=255)                                                                                                                                                                                                                                  tACCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCACTTCATTCCTCCAGTTACGTGAACGCt        >  2:230243/1‑251 (MQ=255)                                                                                                                                                                                                                                   aCCTGCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTGAACGCt        <  1:532394/250‑1 (MQ=255)                                                                                                                                                                                                                                       gCTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCa   <  2:393571/251‑1 (MQ=255)                                                                                                                                                                                                                                        cTCGCCTTTAACGTTCACAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGTAAGTGCCGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCAt  >  2:197943/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           GTTGAATGGACCGTCTGCGCTCATAGACGTGGACGGACGCATTGCGCCGACCATCACCACCGGTTTGTCGCATTTCACCGTCAGGTCGAGGAAGTAAGCAGTTTCTTCCATCGTGTCGGTACCGTGGGTAATGACGAAGCCGTCGGTCTTATCGCAGTCGGTGTTAATTTTTTTCGCCAGTGTCAGCCAGACATTATCGTTCATGTCCTGGGAGCCGATATTCACTACCTGCTCGCCTTTAACGTTCGCAATGTCTTTTAGTTGCGGCACCGCATTAACCAGATTTTCTACGCCAACTTTACCCACTGTGTAGTTAGATTTGGTTGCGGAGTCACCACCACCGGCAATGGTCCCGCCGGTTGCTAAAATGGTGATATTGGGTAATGCCAATGCTGCACCACTAAAACCCATAACCAGTGCGGCAAGTGCCGTCTTTTTGAAAAACTCCATTTCATTCCTCCAGTTACGTGAACGCTACGCAT  >  NC_000913/3100279‑3100760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑