Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,525,090	G→A	L238L (CTC→CTT)	yjhI ←	putative DNA‑binding transcriptional regulator YjhI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,525,090	0	G	A	100.0%	143.9 / NA	44	L238L (CTC→CTT)	yjhI	putative DNA‑binding transcriptional regulator YjhI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/20);  total (24/20)

TGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTG  >  NC_000913/4524846‑4525328                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               tGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAAt                                                                                                                                                                                                                                          >  2:32375/1‑251 (MQ=255)          ttGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATa                                                                                                                                                                                                                                  <  1:298076/250‑1 (MQ=255)           ttgCTAAATTCACCACCGGTCCCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAAGAAGTCGAAAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACTATGAAACGTGCTGGATACCTGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAATTGCTCATTTTTGTTTCTGTATCCCAGCCCTTATGATATTTGCTCATCTCATTTCCGATGCAATTCCATATAg                                                                                                                                                                                                                                 <  2:274206/248‑1 (MQ=255)             gCTAAATTCACCACCGGTACCCAGATAAAACATCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTg                                                                                                                                                                                                                              <  2:340712/251‑1 (MQ=255)                aaaTTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCt                                                                                                                                                                                                                           >  1:111891/1‑251 (MQ=255)                         accGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTAttt                                                                                                                                                                                                                  >  1:609644/1‑251 (MQ=255)                              tACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTg                                                                                                                                                                                                              >  2:535220/1‑250 (MQ=255)                                   aGATAAAACAGCCCGTCGACCCCTTTATTCATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATaa                                                                                                                                                                                                         >  1:509560/1‑250 (MQ=255)                                                   cGACCCCTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAg                                                                                                                                                                                         <  1:349839/250‑1 (MQ=255)                                                       cccTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGt                                                                                                                                                                                    <  2:13955/251‑1 (MQ=255)                                                       cccTTTATTAATCAGGAAGTCGACAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGt                                                                                                                                                                                    >  1:393414/1‑251 (MQ=255)                                                                      gaaggcgacAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCTGTGGAATAATGTCGCTGAATTTTTGCATTACCTCAGTTTCCTTTGCGTAAGTGCTCATTTTAGATTCTGTATCCCAGACCATATGATAGTTGCTCAGCGCACTTGCGAACCAATTCCAGATAGGTGTCTATCACATTACCTGAATAAGCTACCGGATCGCCAGAAGATGAGAATGCATAGTTa                                                                                                                                                                     <  2:84063/243‑1 (MQ=255)                                                                                   tCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATAc                                                                                                                                                         <  2:543679/250‑1 (MQ=255)                                                                                   tCGCGCATTGCCTTTTCATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATAc                                                                                                                                                         <  2:566617/250‑1 (MQ=255)                                                                                      cgcATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACatat                                                                                                                                                     <  1:578926/251‑1 (MQ=255)                                                                                                    aTCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCAt                                                                                                                                        >  2:503147/1‑250 (MQ=255)                                                                                                    aTCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCAt                                                                                                                                        <  1:211367/250‑1 (MQ=255)                                                                                                      cAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACt                                                                                                                                     >  1:148395/1‑251 (MQ=255)                                                                                                       aaGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACtt                                                                                                                                    <  1:32375/251‑1 (MQ=255)                                                                                                        aGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATGCCATCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTa                                                                                                                                   <  2:343802/251‑1 (MQ=255)                                                                                                                gTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATAcc                                                                                                                           >  1:108023/1‑251 (MQ=255)                                                                                                                                 tGGATAACGGTGGAATAATACCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGAttc                                                                                                          >  2:372744/1‑251 (MQ=255)                                                                                                                                   gATACCGGTGGAATAATGCCGCTGAATTTTTTCATTTCCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGAttctt                                                                                                        <  2:440345/251‑1 (MQ=255)                                                                                                                                       ccGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGtt                                                                                                    <  2:151736/251‑1 (MQ=255)                                                                                                                                                  atGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGccc                                                                                         <  2:580647/251‑1 (MQ=255)                                                                                                                                                                      tACCTCAGTTTCCTTTTCGTAAGTGATCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCGCCATAGGTTGATTCTTCGTTGTCTCTGGCCCATCCCCGAAGGCGTGttttt                                                                     >  1:8560/1‑251 (MQ=255)                                                                                                                                                                         cTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGt                                                                  >  2:276966/1‑251 (MQ=255)                                                                                                                                                                           cAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGt                                                                  <  2:509560/249‑1 (MQ=255)                                                                                                                                                                                   ttttCGTAAGTGATCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGATCAGCGCATTTCCGAAGCAATTCCAGATAGATGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGGTGATTCTTCGTTGTCTATGGCCCATCCCCGCAGGCGTGTTTTTTGTAATTCTTcc                                                         >  2:400042/1‑250 (MQ=255)                                                                                                                                                                                    tttCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTcc                                                         <  1:559392/249‑1 (MQ=255)                                                                                                                                                                                        gTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAAc                                                   >  1:284213/1‑251 (MQ=255)                                                                                                                                                                                            gTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAAtt                                               >  2:533981/1‑251 (MQ=255)                                                                                                                                                                                              gCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAAtttt                                             <  1:311322/251‑1 (MQ=255)                                                                                                                                                                                                         tttCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTaa                                   >  1:350062/1‑250 (MQ=255)                                                                                                                                                                                                            cTGTATCGCAGCCCATATGCTATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGt                               >  1:568018/1‑251 (MQ=255)                                                                                                                                                                                                                aTCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTg                           >  2:620241/1‑251 (MQ=255)                                                                                                                                                                                                                     aGCCCATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGt                      >  1:318334/1‑251 (MQ=255)                                                                                                                                                                                                                       cccATATGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGACAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTAt                    >  2:147851/1‑251 (MQ=255)                                                                                                                                                                                                                          atatGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTAtgtg                 >  1:45201/1‑251 (MQ=255)                                                                                                                                                                                                                             tGATATTTGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTgg                >  2:249100/1‑249 (MQ=255)                                                                                                                                                                                                                                    tGCTCAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACg        <  2:547042/250‑1 (MQ=255)                                                                                                                                                                                                                                        caccgcctttCCGAAACAATTCCCGATAGATGTCTATATTATTTCCTGAATCAACTACCGGATCGCCAGAAAGTGAGATTGCATACTTAACTCTGATATACAAATTGAATACCGGCATACTTCAACATACAGCCCCATAGGATGATTCTTCGTTGTCTATGCCACATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGCAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGGNAGt   >  2:595891/8‑251 (MQ=255)                                                                                                                                                                                                                                        cAGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGa     <  1:408118/249‑1 (MQ=255)                                                                                                                                                                                                                                         aGCGCATTTCCGAAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTg  >  1:576370/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               TGTATTCATTTGGCTAAATTCACCACCGGTACCCAGATAAAACAGCCCGTCGACCCCTTTATTAATCAGGAAGTCGGCAACTTCGCGCATTGCCTTTTTATCAAGGGTTCCGTCACGATGAAACGTGCTGGATACCGGTGGAATAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATCCCCGAAGGCGTGTTTTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTG  >  NC_000913/4524846‑4525328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑