Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	850,617	C→T	D56D (GAC→GAT)	ompX →	outer membrane protein X

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	850,617	0	C	T	100.0%	176.2 / NA	56	D56D (GAC→GAT)	ompX	outer membrane protein X
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/28);  total (28/28)

ATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGACAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATACCCGACCTACAAACACGACACCAGCGACTACGGTTTCTCCTACGGTGCGGGTCTG  >  NC_000913/850376‑850857                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   aTCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCg                                                                                                                                                                                                                                          <  1:420750/251‑1 (MQ=255)      gAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTTCGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCttgg                                                                                                                                                                                                                                     >  2:167776/1‑248 (MQ=255)          agTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGtgtg                                                                                                                                                                                                                                 <  1:487992/251‑1 (MQ=255)           gTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGa                                                                                                                                                                                                                                <  2:180678/251‑1 (MQ=255)                 cATTAATTTTTTATATAGTTAAATCTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCGTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGATTTCTACTGTAACTGGCGGTGACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGtt                                                                                                                                                                                                                          <  1:263868/251‑1 (MQ=255)                         tttGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTATTTCACt                                                                                                                                                                                                                  >  1:321435/1‑251 (MQ=255)                         tttGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑ACCGCTGGGAGTGATCGGTTCTTTCACt                                                                                                                                                                                                                  >  2:191517/1‑251 (MQ=255)                          ttGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGCTAGGAACAGAATGGCATGTCTTTAAGCACTGGCCGCAGTTCTGACTTTCACCGCAGGTACTTCCGTAACTGCGACTTCTACTGTAACTGGCGGGTACGCACAGGGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑CTTCAACCTGAAATACCGCTCTGAGGAAGATAACGG‑CCAGCTGGGGGATATCGGGTCTTTCACtt                                                                                                                                                                                                                 >  1:50930/1‑251 (MQ=255)                               atatTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACAcc                                                                                                                                                                                                            <  2:541810/251‑1 (MQ=255)                                aatTTAAAACTTTAGACTTAATTGAAACACCTTTGAGTTTGTTATGAAAAAAATTGCATTTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACAcc                                                                                                                                                                                                            <  2:354077/249‑1 (MQ=255)                                        aCTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACTCACAGAGCGACGCTCAGGGCCAACTGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACCCCGAGACAAGc                                                                                                                                                                                                   >  1:452072/1‑251 (MQ=255)                                                   aTTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCCACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGGGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTAATGCa                                                                                                                                                                                          >  1:391565/1‑249 (MQ=255)                                                    tttGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTc                                                                                                                                                                                                                     <  1:210588/221‑1 (MQ=255)                                                    tttGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGGGTGATCGGTTCTTTc                                                                                                                                                                                                                     >  2:210588/1‑221 (MQ=255)                                                            acaTTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGGTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTgg                                                                                                                                                                               >  1:643817/1‑250 (MQ=255)                                                                 tGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAAACTGAAATAACGCCATGAAGAAGATAAAAG‑GCCGCTGGGGGTGATCGGTTATTTCACTTAAACCCAGAAAAACAGGACTGCCAGCTCTGGTtactac                                                                                                                                                                           >  2:337557/1‑250 (MQ=255)                                                                    ggtggtTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTacaaca                                                                                                                                                                       <  1:209489/251‑1 (MQ=255)                                                                    ggtggtTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTacaaca                                                                                                                                                                       <  1:484362/251‑1 (MQ=255)                                                                     gtggtTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAAAAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAGACCCG‑TGGGGGTGATCGGTTCTTTCACTTACAACGAGAAAAGCCGTACATCAAGCTCTGGTGACTacaaaa                                                                                                                                                                       >  2:316548/1‑248 (MQ=255)                                                                       ggtTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGTCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACaaaa                                                                                                                                                                    <  1:129065/251‑1 (MQ=255)                                                                              aaaaaaaTTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGACCTGGTGACTTCAACAAAAACCA‑Gt                                                                                                                                                             >  2:353491/1‑250 (MQ=255)                                                                               aaaaaaTTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGGGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑Gtac                                                                                                                                                           >  1:508033/1‑251 (MQ=255)                                                                                           gTCTTGCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTGCAACCTGAAATACCGCTATGATGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCTAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCAct                                                                                                                                               <  1:420543/251‑1 (MQ=255)                                                                                                tCAGCAATGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCCCAGAGGGAAGCGCAGGGCCACCTGAACAAAATGGGGGGGTTTCA‑CCGGAAATACCGCCCAGAAGACGATAACAG‑ACCCCTGGGTGTGATCGGTTCTTTTCCTTACCCCCATCACACCCTGACGACAAGCTCTGGTGACTACCACAAAA‑CCACGTACTACggaccacacgccgg                                                                                                                                          >  2:462950/1‑239 (MQ=255)                                                                                                   gCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGc                                                                                                                                                                                           <  1:616673/200‑1 (MQ=255)                                                                                                   gCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGc                                                                                                                                                                                           >  2:616673/1‑200 (MQ=255)                                                                                                     aCTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCAAAGAGCGACGCGCAGGGCCAAATGAAAAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGGGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTAATGCAAGCTCTGGTGAATACAACAAAAACCA‑ATACTACGGCATCACTGCTGGTCCgg                                                                                                                                     >  2:374852/1‑251 (MQ=255)                                                                                                             cAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGc                                                                                                                             <  1:581089/251‑1 (MQ=255)                                                                                                             cAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGc                                                                                                                             <  2:391565/251‑1 (MQ=255)                                                                                                               gTTCTGGCTTTCACCGCAGGAACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAAATAACAG‑CCCGCTGGGTGTGCTCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGGGACTACAACAAAAACCA‑GCAACACGGCATCACTGCTGGGCCGGCTTACCGCAt                                                                                                                           >  1:463196/1‑251 (MQ=255)                                                                                                                        ttCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCCTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTg                                                                                                                  >  1:137335/1‑251 (MQ=255)                                                                                                                        ttCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATAGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCACGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTg                                                                                                                  >  1:248085/1‑251 (MQ=255)                                                                                                                          cACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTaca                                                                                                                                                                          >  2:1080/1‑194 (MQ=255)                                                                                                                          cACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTaca                                                                                                                                                                          <  1:1080/194‑1 (MQ=255)                                                                                                                          cACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTgg                                                                                                                 <  1:374852/250‑1 (MQ=255)                                                                                                                                             gCTGAGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATCCCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAATCCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAgt                                                                                             <  2:502605/251‑1 (MQ=255)                                                                                                                                                 cGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAgtgggt                                                                                         <  2:508033/251‑1 (MQ=255)                                                                                                                                                      tCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAgtgggtgtgg                                                                                     <  2:514040/250‑1 (MQ=255)                                                                                                                                                         aCTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCGGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTGCACTTACACCGAGAAAAGCCGTACTTCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAgtgggtgtggg                                                                                    <  2:213802/248‑1 (MQ=255)                                                                                                                                                                  gaagggtacacacaGAGCCACGCGCAGGGGCAAATGAACAAAAGGGGCGT‑CTTCAAACTGAAATACCGCTATGAAGAAGATAACAA‑CCCGCTGGGTGTGATCCGTTCTGCCACGTACCCCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACAGCTGGTCCAGCATACCGTATTAACGACTTGGCAAGCTTCTACTGTTTAGTGGGTCTGGGTTATggaaac                                                                          <  2:440866/239‑5 (MQ=255)                                                                                                                                                                             acaGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTg                                                             <  1:191517/251‑1 (MQ=255)                                                                                                                                                                                gagggaagCTCAGGGCTAAATTAACAAAATGGTCTG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTTGTGACTACAACAAAAACCA‑GGACTACGGCATCGCTGCTGGTCCGGCTTACCTCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGTCCACTGAAt                                                          <  2:360336/244‑1 (MQ=255)                                                                                                                                                                                  gCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATAc                                                        >  1:184001/1‑251 (MQ=255)                                                                                                                                                                                    gACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACTACTGGGCAAGCATCTACGGGGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATAccc                                                      >  2:420470/1‑251 (MQ=255)                                                                                                                                                                                    gACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATAccc                                                      >  1:44598/1‑251 (MQ=255)                                                                                                                                                                                      cGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGAATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATACCCg                                                     >  2:433977/1‑250 (MQ=255)                                                                                                                                                                                         tCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATACCCGACCt                                                 >  2:76079/1‑251 (MQ=255)                                                                                                                                                                                                cAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTGCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCGCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGGGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATATCCGTCCTACAAaca                                          <  2:387290/251‑1 (MQ=255)                                                                                                                                                                                                     gAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATACCCGACCTACAAACACGac                                      >  2:509473/1‑250 (MQ=255)                                                                                                                                                                                                                 cGG‑TTTCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATACCCGACCTACAAACACGACACCAGCGACTACg                         <  1:76079/251‑1 (MQ=255)                                                                                                                                                                                                                      ttCAACCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATACCCGACCTACAAACACGACACCAGCGACTACGGtt                      <  2:184001/250‑1 (MQ=255)                                                                                                                                                                                                                          aCCTGAAATACCGCTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATACCCGACCTACAAACACGACACCAGCGACTACGGTTTNTcc                 >  2:586412/1‑251 (MQ=255)                                                                                                                                                                                                                                       cTATGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATACCCGACCTACAAACACGACACCAGCGACTACGGTTTCTCCTACGGTGCGGGTc    >  1:13816/1‑251 (MQ=255)                                                                                                                                                                                                                                         aTGAAGAAGATAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATACCCGACCTACAAACACGACACCAGCGACTACGGTTTCTCCTACGGTGCGGGTCTg  <  1:118002/251‑1 (MQ=255)                                                                                                                                                                                                                                              gaagaTAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATACCCGACCTACAAACACGACACCAGCGACTACGGTTTCTCCTACGGTGCGGGTCt   >  1:607935/1‑245 (MQ=255)                                                                                                                                                                                                                                              gaagaTAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCAGCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATACCCGACCTACAAACACGACACCAGCGACTACGGTTTCTCCTACGGTGCGGGTCt   <  2:607935/245‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   ATCGCGAAGAGTTTCCCATTAATTTTTGATATATTTAAAACTTAGGACTTATTTGAATCACATTTGAGGTGGTTATGAAAAAAATTGCATGTCTTTCAGCACTGGCCGCAGTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCTCAGGGCCAAATGAACAAAATGGGCGG‑TTTCAACCTGAAATACCGCTATGAAGAAGACAACAG‑CCCGCTGGGTGTGATCGGTTCTTTCACTTACACCGAGAAAAGCCGTACTGCAAGCTCTGGTGACTACAACAAAAACCA‑GTACTACGGCATCACTGCTGGTCCGGCTTACCGCATTAACGACTGGGCAAGCATCTACGGTGTAGTGGGTGTGGGTTATGGTAAATTCCAGACCACTGAATACCCGACCTACAAACACGACACCAGCGACTACGGTTTCTCCTACGGTGCGGGTCTG  >  NC_000913/850376‑850857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑