Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,928,607	C→T	G333S (GGC→AGC)	viaA ←	protein ViaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,928,607	0	C	T	96.3%	252.3 / ‑4.0	82	G333S (GGC→AGC)	viaA	protein ViaA
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base T (48/30);  total (51/31)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGACCATCAACCT  >  NC_000913/3928357‑3928852                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      gCATCGGCCTCAACCCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGACAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGTGCCTG‑AAAGCTCATAACGGACTCCCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAgccgcc                                                                                                                                                                                                                                                       >  2:41543/1‑251 (MQ=255)           cAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGcc                                                                                                                                                                                                                                              <  1:292883/250‑1 (MQ=255)            aaaCCATTCCCTGCTTTGCAAGCGTTCCATAATGGAGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAACCGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCCAACTGTTCATTAAAGCCGCTCATTGAGCCgg                                                                                                                                                                                                                                            >  2:139716/1‑251 (MQ=255)             aaCCATTCCCTGCTTTGCAAGCGTTCGATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCgg                                                                                                                                                                                                                                            >  2:484023/1‑250 (MQ=255)                           ttGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCcacac                                                                                                                                                                                                                                >  2:440390/1‑248 (MQ=255)                            tGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGAc                                                                                                                                                                                                                            >  1:298278/1‑251 (MQ=255)                              cAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACa                                                                                                                                                                                                                           >  1:450626/1‑250 (MQ=255)                               aaGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAAt                                                                                                                                                                                                                         <  1:440390/251‑1 (MQ=255)                                     tCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGCAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCACGGCCCGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATaaaagg                                                                                                                                                                                                                   >  2:576660/1‑248 (MQ=255)                                        aTAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCAAACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGAAAATAAACGGccc                                                                                                                                                                                                                >  1:179918/1‑251 (MQ=255)                                                  gAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGt                                                                                                                                                                                                      >  1:481873/1‑251 (MQ=255)                                                  aaaaaCAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAGAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATTCGCATCAAGGCCATGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGTCCCGCGCGGCTGt                                                                                                                                                                                                      <  1:275648/250‑1 (MQ=255)                                                  aaaaaCAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGt                                                                                                                                                                                                      <  2:431554/250‑1 (MQ=255)                                                     aacaacTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTggtc                                                                                                                                                                                                    >  1:194618/1‑247 (MQ=255)                                                      acaacTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGAATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACACTAAACGGCCCGCGCGGCTGTTCAt                                                                                                                                                                                                  >  2:373198/1‑251 (MQ=255)                                                       caacTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATc                                                                                                                                                                                                 >  1:382467/1‑251 (MQ=255)                                                       caacTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATc                                                                                                                                                                                                 >  1:225000/1‑251 (MQ=255)                                                            ggCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAAt                                                                                                                                                                                            >  1:643527/1‑251 (MQ=255)                                                             gCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATc                                                                                                                                                                                           <  2:347757/251‑1 (MQ=255)                                                             gCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATc                                                                                                                                                                                           <  1:512627/251‑1 (MQ=255)                                                                aGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCttt                                                                                                                                                                                        <  1:273472/251‑1 (MQ=255)                                                                             cACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCgg                                                                                                                                                                            >  1:190522/1‑250 (MQ=255)                                                                              aCGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCt                                                                                                                                                                                                        >  1:387498/1‑221 (MQ=255)                                                                              aCGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATGAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAGCGGCCCGCGCGGCt                                                                                                                                                                                                        <  2:387498/221‑1 (MQ=255)                                                                                 aaaCTGCTGGCTTTAAAAACGGATTGCTTGTTCGATG‑CCGTGTGGGCCTG‑AAAGCTCATAACGGACAATCTCTGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCCTGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTTTCCACTCAGTCAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGtt                                                                                                                                                                       <  2:28066/251‑1 (MQ=255)                                                                                      gctgGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATACTATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTa                                                                                                                                                                                              >  1:598286/1‑223 (MQ=255)                                                                                      gctgGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGAt                                                                                                                                                                   <  1:180889/250‑1 (MQ=255)                                                                                      gctgGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATc                                                                                                                                                                  >  1:299465/1‑251 (MQ=255)                                                                                      gctgGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCGG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATTCGCAGCAAGGCCAGGCAGAACGCGTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCTGCTGTTCATCGTa                                                                                                                                                                                              <  2:598286/223‑1 (MQ=255)                                                                                           cTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGACGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTAGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGATGTTCATCGAAATCTTTATGTACCACAGGACGTTCGATCCCttt                                                                                                                                                             >  2:452189/1‑251 (MQ=255)                                                                                                aaaaCGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCAc                                                                                                                                                        >  2:480316/1‑251 (MQ=255)                                                                                                   aCGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGcc                                                                                                                                                     <  2:553564/251‑1 (MQ=255)                                                                                                     ggATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCAc                                                                                                                                                   >  2:333471/1‑251 (MQ=255)                                                                                                     ggATTGCTTGTGCG‑TGTCCTTGTGGGC‑TGTAAAGCTCATAACGGACGAGCTCGTTGGAAAAAAGCATAATATAGAAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACTCTTTCGCACACTTTTCATTAAATCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCCCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGACCTCTTTTTCACGCCAc                                                                                                                                                   <  2:417525/251‑1 (MQ=255)                                                                                                                   aTG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGc                                                                                                                                       >  1:338124/1‑249 (MQ=255)                                                                                                                        cTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCg                                                                                                                                  >  1:296880/1‑250 (MQ=255)                                                                                                                         ttGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGAc                                                                                                                                                                          >  2:130706/1‑209 (MQ=255)                                                                                                                         ttGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGAc                                                                                                                                                                          <  1:130706/209‑1 (MQ=255)                                                                                                                          tgtgGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGACAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATACACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGAt                                                                                                                                >  1:277588/1‑250 (MQ=255)                                                                                                                          tgtgGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGa                                                                                                                                 <  2:338124/249‑1 (MQ=255)                                                                                                                          tgtgGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGAATCCACACAGACACTAAACGGCCCGCACGGCTGTTCATCGTAATCTTTACGTACCACCGTACGTTCGTTCACTTTTTCACGCCACGACTCACCGTGCAGGCGAt                                                                                                                                >  1:70742/1‑250 (MQ=255)                                                                                                                           ttggaccTG‑AAAGTGCATAACGGACGACATGGTTGGAAAAGAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAGGCCGCTCATTGAGCCGAAAGTATCCGCACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACATTTTAACGCCACGACTCACCGTGCAGGCGATa                                                                                                                               <  2:466920/245‑1 (MQ=255)                                                                                                                              ggCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTg                                                                                                                           <  1:549744/251‑1 (MQ=255)                                                                                                                              ggCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCACCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTg                                                                                                                           <  2:282865/251‑1 (MQ=255)                                                                                                                               gCCTG‑AAAGCTCATAACGGACGATCTCGGCGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTg                                                                                                                           >  1:523560/1‑250 (MQ=255)                                                                                                                                ccTG‑AAAGCTCATAACGGAAGATCTCGGTGTAAAATAGCATAATATAGCAGCGCCTGTTTTCTGCGAGAGAAATGCGCATCAAGGCCAGGCAGAACCCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTCCCACCGGACGTTCGATCCCTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAg                                                                                                                         <  2:225627/251‑1 (MQ=255)                                                                                                                                          tCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTacca                                                                                                                                                                                <  1:60041/187‑1 (MQ=255)                                                                                                                                          tCATAACGGACGATATCGGCGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTacca                                                                                                                                                                                >  2:60041/1‑187 (MQ=255)                                                                                                                                              aaCGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTc                                                                                                             <  2:543605/250‑1 (MQ=255)                                                                                                                                                cGGACGATCTCGGTGGAAAAAAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTCCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTAGCCACACAGACAATAAAGGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTCCCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTccac                                                                                                          <  2:391585/251‑1 (MQ=255)                                                                                                                                                 ggACGATCTCGGTGGAAAATAGCATAATATAGCAGAGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGCAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTccacc                                                                                                         >  2:136101/1‑251 (MQ=255)                                                                                                                                                  gACGATCTCGGTGTAAAATAGCATAAAATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATAAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTGTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTccacca                                                                                                        <  1:552635/251‑1 (MQ=255)                                                                                                                                                      atatcGGTGGACAGTAGCATAAAATAGCAGCGCCGGTTTTCTGCGCGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGATCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATCAACCGCCCGCGCGGCTGTTCATCGAACTCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATATGTGAGCAACTGTTTTTCCACCAGCCg                                                                                                    >  2:274717/4‑251 (MQ=255)                                                                                                                                                          cGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCTCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAGCTGTTTTTCCACCAGcc                                                                                                     <  1:535785/246‑1 (MQ=255)                                                                                                                                                           ggTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGt                                                                                               >  2:47900/1‑251 (MQ=255)                                                                                                                                                                aaaaTAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAAc                                                                                          >  2:349075/1‑251 (MQ=255)                                                                                                                                                                 caaTAGCATAATATAGCATCGCCGGGTTTCTGAGAGATAAATGCGCATCAAGGCCAGGCAGAACGCTTTCTCACACTGTTGATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTAACACCGAACGTTCGAACACTTTTTCACGCCACGACGCACCGTGCAGGCGATAGGAGAGCAACTGTTTTTACACCAGCCGTCGGTAAAACt                                                                                         >  2:17661/2‑251 (MQ=255)                                                                                                                                                                     aGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCAt                                                                                      >  2:412370/1‑250 (MQ=255)                                                                                                                                                                     aGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATa                                                                                     <  1:446553/251‑1 (MQ=255)                                                                                                                                                                           atatAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTcc                                                                                 >  2:433214/1‑249 (MQ=255)                                                                                                                                                                                 cagcGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGt                                                                         >  1:358559/1‑251 (MQ=255)                                                                                                                                                                                  agcGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGtt                                                                        >  2:113139/1‑251 (MQ=255)                                                                                                                                                                                        ggTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAACACTCATACTCCAGTTCCGTTATCCCt                                                                  >  2:310861/1‑251 (MQ=255)                                                                                                                                                                                         gTTTTCTGCGAGAGCAATGCGCAACAAGGCAAGGCAGAACGCTTTCGCACACTGTGCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACAACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACGGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTa                                                                 <  1:30819/251‑1 (MQ=255)                                                                                                                                                                                              cTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTc                                                            >  2:186002/1‑251 (MQ=255)                                                                                                                                                                                                  gagaGCAATGCGCATCCAGGCCAGGAAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAACCGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGccc                                                        >  2:464437/1‑250 (MQ=255)                                                                                                                                                                                                   agagCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGcc                                                         >  1:483977/1‑249 (MQ=255)                                                                                                                                                                                                     agCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATCCTCCAGTTCCGTTATCCCTAGTGTCGcc                                                         >  2:516588/1‑247 (MQ=255)                                                                                                                                                                                                          tGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTggc                                                >  2:173884/1‑251 (MQ=255)                                                                                                                                                                                                           gcgcATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTggcg                                               <  1:270861/251‑1 (MQ=255)                                                                                                                                                                                                             gcATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTCGTGTCGCCAGTTCTggcggc                                             >  2:261489/1‑251 (MQ=255)                                                                                                                                                                                                                tCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAgg                                          >  1:74768/1‑251 (MQ=255)                                                                                                                                                                                                                 cAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGga                                         <  1:333471/251‑1 (MQ=255)                                                                                                                                                                                                                    ggCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGAc                                      >  2:610349/1‑251 (MQ=255)                                                                                                                                                                                                                       cAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGt                                    >  1:524407/1‑250 (MQ=255)                                                                                                                                                                                                                           cAGAACGCTTTCGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAat                               >  1:225614/1‑251 (MQ=255)                                                                                                                                                                                                                                 gCTTTCGCACACGGGTCATTAAAGCCGCGCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATatcata                         <  2:635055/251‑2 (MQ=255)                                                                                                                                                                                                                                      cGCACACTGTTCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCCCCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCttt                    >  2:543743/1‑251 (MQ=255)                                                                                                                                                                                                                                                tCATTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGAcc          >  2:3794/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cATNAAAGCCGCTGATGGAGCCGGAAGTATCCACCCCTACAATAAACGGCCAGCGCTGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGTGATAGGTGAGCAACTGTTGTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTAGCCCTAGTGTCGCCAGGTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGTAGACCa         <  2:591536/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aTTAAAGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCACGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTGTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGTGGACGTAAAATATCATCGCTTTGCTGCAGAccct        >  1:225855/1‑249 (MQ=255)                                                                                                                                                                                                                                                   ttAAAGCCGCTCATTGAGCAGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGACCATc       >  1:551091/1‑251 (MQ=255)                                                                                                                                                                                                                                                       aGCCGCTCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCTTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGACCATCAAcc   <  1:473833/251‑1 (MQ=255)                                                                                                                                                                                                                                                        gccgctCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGACCATCAACCt  >  1:364041/1‑251 (MQ=255)                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      GCATCGGCATCAAACCATTCCCTGCTTTGCAAGCGTTCCATAATGGCGCGAAAACAACTGGCAAGATCGGTGCCGCCACGAAACTGCTGGCTTAAAAAACGGATTGCTTGTTCGATG‑CCTTGTGGGCCTG‑AAAGCTCATAACGGACGATCTCGGTGGAAAATAGCATAATATAGCAGCGCCGGTTTTCTGCGAGAGCAATGCGCATCAAGGCCAGGCAGAACGCTTTCGCACACTGTTCATTAAAGCCGCCCATTGAGCCGGAAGTATCCACACAGACAATAAACGGCCCGCGCGGCTGTTCATCGTAATCTTTATGTACCACCGGACGTTCGATCACTTTTTCACGCCACGACTCACCGTGCAGGCGATAGGTGAGCAACTGTTTTTCCACCAGCCGACGGTAAAACTCATACTCCAGTTCCGTTATCCCTAGTGTCGCCAGTTCTGGCGGCAGGAGACGTAAAATATCATCGCTTTGTTGCAGACCATCAACCT  >  NC_000913/3928357‑3928852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑