Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,245,238	G→A	I394I (ATC→ATT)	malE ←	maltose ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,245,238	0	G	A	100.0%	220.7 / NA	62	I394I (ATC→ATT)	malE	maltose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/27);  total (35/27)

CACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCC  >  NC_000913/4244995‑4245472                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           caccaGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTACTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACg                                                                                                                                                                                                                                       >  2:613439/1‑249 (MQ=255)    cagcagGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCt                                                                                                                                                                                                                                  >  1:402041/1‑251 (MQ=255)     ggcagGCCGAGCAGACCTAGCCCGGACCTTTTCAGCGCGTCGCTTTGCCCCCAATGTTTCTTTTGACTTACATTCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATACGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACACCGCATCAGGCATTTCACAGCATTACTTGCTAATACGAGTCTg                                                                                                                                                                                                                                 >  2:128880/2‑251 (MQ=255)            cGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGCCGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCt                                                                                                                                                                                                                          >  1:389515/1‑251 (MQ=255)                    ccTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGc                                                                                                                                                                                                                  >  2:641993/1‑251 (MQ=255)                      aaGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAAGGTTTCTGTTTAATGACACCCATGGGGTTCTGCCTCATTCCGGGACGGCTAAGGCTTTCACGCCTTATCCGCCAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGTGCtt                                                                                                                                                                                                                <  2:394487/250‑1 (MQ=255)                                  gttCAGCGCGTCGCTTTGACACCACTTTTTCTTTTTAATGACATCCTTGGGTGTCTTCCGCACTCCCGGACGTATAAGCTTTTCACGTCTTATTCGACAACAACTGCCTGATGCCACGTTGACGCGTCTTATCAGGCCTACCTCAGTTTCGGTTTTGTAGGTCGGACAAGGCTCGCACGTTGTATCCGGTTTTTTACATCATGACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGt                                                                                                                                                                                                      <  1:75077/248‑1 (MQ=255)                                         gcgTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCAGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGAcc                                                                                                                                                                                             >  2:379416/1‑251 (MQ=255)                                                     caccaATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACTACCGCTGGCGGCGtt                                                                                                                                                                                 <  1:174414/251‑1 (MQ=255)                                                                      aaTGACATCCATGGGGTTCTTCCTCATGCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGTGCGTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCa                                                                                                                                                                <  2:315697/251‑1 (MQ=255)                                                                        tGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACg                                                                                                                                                              >  1:171528/1‑251 (MQ=255)                                                                        tGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACg                                                                                                                                                              >  1:572402/1‑251 (MQ=255)                                                                          aCATCCATGGGGTTCTTCCTCTTTCCAGGACTGATAAGGCTTTCACGCCTTATCCTACAACAACTGCTTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGc                                                                                                                                                            <  2:23589/251‑1 (MQ=255)                                                                             agcaggggtgttttcCTCATGCCAGGATTGATAGGTCTTGCACGTCTTATCCGACAACAGCTGCCTGATGCGTCGTTGACGCGTCTTATCAGCCCTACATACGTTTTGGTTTTTTAGGCCTGACAAGGCGTTCACGCCGCATCCGTCATTTCTCAGCATGACTTGGTAATACTAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCACTGGCGGCGTTGATCACCGCAGTCCGCaatgcata                                                                                                                                                         <  2:412733/239‑8 (MQ=255)                                                                                aTGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATAcc                                                                                                                                                       >  2:465819/1‑250 (MQ=255)                                                                                aTGGGGTTCTTCCTCATTCCAGGACGAATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGAATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATAcc                                                                                                                                                       >  1:230700/1‑250 (MQ=255)                                                                                    gtttcttcCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACACCAACTGCCTGATGCGACTCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGTACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATTCGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCTGCGTTGATCACCGCAGTACGCACGGCATACCAGaaa                                                                                                                                                  <  1:194985/249‑1 (MQ=255)                                                                                    ggTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGCTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGaa                                                                                                                                                   >  1:35834/1‑250 (MQ=255)                                                                                            cTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACAt                                                                                                                                          >  1:34419/1‑251 (MQ=255)                                                                                              catgccAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCGGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCt                                                                                                                                        <  1:261340/247‑1 (MQ=255)                                                                                              cATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACTCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACAACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCt                                                                                                                                        <  2:10655/251‑1 (MQ=255)                                                                                                               ggCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATg                                                                                                                       <  1:492597/251‑1 (MQ=255)                                                                                                                   ccgcatccggcaacagtgccaacTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGAttt                                                                                                                   <  1:147731/232‑1 (MQ=255)                                                                                                                                 gACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCg                                                                                                     >  1:333950/1‑251 (MQ=255)                                                                                                                                    aacaacTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGCCAAGGCGTTAACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAATCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGAACGCTGGCGGAGTTGATCACCGCAGTACGCACGGCATACCAGCAAGCGGACATCTGCGGGCTGTTCGACATGATTACACCTTTCTGGGCGttt                                                                                                  >  2:496534/1‑251 (MQ=255)                                                                                                                                     acaacTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGtttt                                                                                                 >  2:639328/1‑251 (MQ=255)                                                                                                                                      caacTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTc                                                                                                >  1:174723/1‑251 (MQ=255)                                                                                                                                                aTGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTggcg                                                                                      <  1:157798/251‑1 (MQ=255)                                                                                                                                                    gACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCa                                                                                   >  2:113945/1‑250 (MQ=255)                                                                                                                                                      cGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATa                                                                                <  2:445629/251‑1 (MQ=255)                                                                                                                                                                 cTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCttt                                                                     >  2:442791/1‑251 (MQ=255)                                                                                                                                                                  ttATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTc                                                                    >  1:28056/1‑251 (MQ=255)                                                                                                                                                                   tATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCtt                                                                      <  1:197221/248‑1 (MQ=255)                                                                                                                                                                    aTCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGTCAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCg                                                                   <  2:572402/250‑1 (MQ=255)                                                                                                                                                                     tCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGcc                                                                 >  1:285752/1‑251 (MQ=255)                                                                                                                                                                      cAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGcc                                                                 <  1:613439/250‑1 (MQ=255)                                                                                                                                                                      cAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCa                                                                <  1:340788/251‑1 (MQ=255)                                                                                                                                                                             aCATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAActct                                                           <  1:654976/249‑1 (MQ=255)                                                                                                                                                                             aCATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTc                                                         >  2:510078/1‑251 (MQ=255)                                                                                                                                                                                 aCGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCg                                                     <  1:375726/251‑1 (MQ=255)                                                                                                                                                                                        ggTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACt                                              <  1:484339/251‑1 (MQ=255)                                                                                                                                                                                         gTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACtt                                             >  2:575640/1‑251 (MQ=255)                                                                                                                                                                                         gTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCAGGCATTTCACAGCATTACTTGGGAATACGAGTCTGCGCGTCTTTCAGGACTTCATCGAAAGTCTGAAGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTGCGGCATGATTTCGCCTTTCTGAGCGTTTTCCATGGTGGCGGAAATACGTGGATCTTTCGCCAAATCTTCCTCGCAAGACtt                                             >  2:352086/1‑251 (MQ=255)                                                                                                                                                                                                   ccGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACg                                   <  1:311686/251‑1 (MQ=255)                                                                                                                                                                                                    cGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACgg                                  >  1:2903/1‑251 (MQ=255)                                                                                                                                                                                                    cGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACgg                                  >  2:86676/1‑251 (MQ=255)                                                                                                                                                                                                              gTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCgg                        >  1:79366/1‑251 (MQ=255)                                                                                                                                                                                                                 cACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTc                                                                                                                  <  2:158402/158‑1 (MQ=255)                                                                                                                                                                                                                 cACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTc                                                                                                                  >  1:158402/1‑158 (MQ=255)                                                                                                                                                                                                                 cACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAg                           >  1:84031/1‑245 (MQ=255)                                                                                                                                                                                                                 cACGCCGCATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAg                           <  2:84031/245‑1 (MQ=255)                                                                                                                                                                                                                   cgccgcATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGt                   >  1:149885/1‑251 (MQ=255)                                                                                                                                                                                                                    gccgcATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTc                  >  1:510594/1‑251 (MQ=255)                                                                                                                                                                                                                    gccgcATCCGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTc                  >  1:334304/1‑251 (MQ=255)                                                                                                                                                                                                                    gccgcATCCGGCATGTCACAGCATTTCTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTGCATCGACAGTCTGACGTCCGCTGGCGGCGTTGATCGCCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCCCCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGTTTTGTc                  <  2:288223/251‑1 (MQ=255)                                                                                                                                                                                                                           ccGGCATTTCACAGCATTTCTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGAGGCAATACGTGTATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatta           <  1:301699/251‑1 (MQ=255)                                                                                                                                                                                                                           ccGGCATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTttatt            >  1:463343/1‑250 (MQ=255)                                                                                                                                                                                                                               cATTTCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCg       >  1:193047/1‑251 (MQ=255)                                                                                                                                                                                                                                   tCACAGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACAGCTGCGGGATGTTCGGCATGATTTCCCCTTTCTGGGCGTTTTCCATGGTGGCGGCAATTCGTGGATCTTTCGCCAACTCTTCCGCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTGTATTAGCCGCtt    <  2:156614/250‑1 (MQ=255)                                                                                                                                                                                                                                     acaGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCa                                >  1:587834/1‑220 (MQ=255)                                                                                                                                                                                                                                     acaGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCa                                <  2:587834/220‑1 (MQ=255)                                                                                                                                                                                                                                      caGCATTACTTGGTAATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACAGCTTcc  >  1:613236/1‑249 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           CACCAGCAGGCCGAGCAGACCTAGCACTGACCATTTCAGCGCGTCGCTTTGCCACCAATGTTTCTTTTTAATGACATCCATGGGGTTCTTCCTCATTCCAGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACATACGTTTCGGTTTTGTAGGCCGGACAAGGCGTTCACGCCGCATCCGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCC  >  NC_000913/4244995‑4245472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑