Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,454,328 C→T A249V (GCC→GTC)  yjfF → galactofuranose ABC transporter putative membrane subunit YjtF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,454,3280CT91.9% 105.7 / ‑5.9 39A249V (GCC→GTC) yjfFgalactofuranose ABC transporter putative membrane subunit YjtF
Reads supporting (aligned to +/- strand):  ref base C (1/1);  major base T (10/24);  minor base G (2/1);  total (13/26)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAG  >  NC_000913/4454086‑4454572
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       
gATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTg                                                                                                                                                                                                                                                  <  1:352038/250‑1 (MQ=255)
gATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTg                                                                                                                                                                                                                                                  <  2:10149/250‑1 (MQ=255)
                      ggCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGCCCTTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGGTTGTCTTCTCCGTTTTTAACCAAGCCGGATATGCGCTGGCGGGCGCGGGTGTGGGAATGGATGCTCTCGTCTCCGTGGTTAATTG‑CGGTACGCCTTTgtg                                                                                                                                                                                                                          >  1:105040/1‑249 (MQ=255)
                        cATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCAGTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGTGCGTAGGTGTGGAGCTGGATTCTATCGTCTCAGTGAT‑AATTGGTGGTACGCTTTTG‑AGCg                                                                                                                                                                                                                       <  2:385849/251‑1 (MQ=255)
                                   cgcATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGa                                                                                                                                                                                                            <  2:106589/251‑1 (MQ=255)
                                     cATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACGGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAAc                                                                                                                                                                                                          <  2:132468/251‑1 (MQ=255)
                                          tACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGGAGGGGGGGAACTGGATGCTATCGGCTCAGTGGG‑AATTGGCGGGACGCTTTTT‑AGCggggggggtgggacgggtt                                                                                                                                                                                                     >  1:176894/1‑234 (MQ=255)
                                           aCCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTAt                                                                                                                                                                                                     <  2:176894/250‑1 (MQ=255)
                                                          cAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTtgg                                                                                                                                                                                     <  2:194494/251‑1 (MQ=255)
                                                                 aCGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTtggcgtggcg                                                                                                                                                                              <  1:336212/251‑1 (MQ=255)
                                                                            ggcAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGGGTTGGGGTGGGACTGGATGCTATTGTCTCCGTGGG‑AATTGGGGGGACGCCTTTGTGGGGGGGGGGTTGGAA‑GGGATTTGGGACGCTTTTttgcgggggggttccggggct                                                                                                                                                                   >  1:292346/1‑235 (MQ=255)
                                                                                       gTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATGCGCATTTATATGCTCTCCGCCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGTGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATGAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACtt                                                                                                                                                        <  1:645001/251‑1 (MQ=255)
                                                                                                   gATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACtt                                                                                                                                              <  2:202177/249‑1 (MQ=255)
                                                                                                       gggATTTCCACTCGCAGCACCACTATTCGGGTTTATATGCGCTCGACCGGACTGGCAACGCTGGCGGGGATTGTCTTCGCGATTTATACCCAGGACGGATATGCGCGGGCGGGCGTAGGTGTGGAGCGGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGGTGGGACGGTATTAGGGAGGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGaggg                                                                                                                                        <  2:478103/251‑4 (MQ=255)
                                                                                                         gATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AAGTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCa                                                                                                                                      <  1:424549/251‑1 (MQ=255)
                                                                                                                aCTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAg                                                                                                                               <  2:177257/251‑1 (MQ=255)
                                                                                                                                                  ccACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTAtttt                                                                                             <  1:521690/250‑1 (MQ=255)
                                                                                                                                                  ccACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTAtt                                                                                               <  1:368020/248‑1 (MQ=255)
                                                                                                                                                         aCTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTa                                                                                     <  1:548572/251‑1 (MQ=255)
                                                                                                                                                            ggCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTAtt                                                                                   <  1:292349/250‑1 (MQ=255)
                                                                                                                                                             gCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTAtttt                                                                                 <  2:498757/251‑1 (MQ=255)
                                                                                                                                                               aaCGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCtggt                                                                                                                       <  1:483543/212‑1 (MQ=255)
                                                                                                                                                                aCGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTTAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTATTTTtat                                                                              <  2:26863/251‑1 (MQ=255)
                                                                                                                                                                 cGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTATTTTtata                                                                             <  1:26723/251‑1 (MQ=255)
                                                                                                                                                                         gggATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCCGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTTGAACGGAATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCCTCGGGATTTTGTTGTTTATTTTTATAGCAtttc                                                                      >  1:464453/1‑248 (MQ=255)
                                                                                                                                                                         gggATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACa                                                                     <  1:283418/251‑1 (MQ=255)
                                                                                                                                                                             ttGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGGACGGGATTAGGGACGCTTTTTGGCGGGGCGATTCAGGGACTGAGTCAGACTTACATAAACTTTGATGGGACGCTGGGTTTCTTGTGGGC‑GAAAAACGCCATTGGTATTTTGTTGTTTATTTTTTTAGCATTTCAGCgg                                                                 >  2:535298/1‑250 (MQ=255)
                                                                                                                                                                                 cttctCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGAAGGTGTGGAAATGGATGCTATAGGCTCAGTGGT‑AATTGGCGGGACGCTTTTG‑TGCGGGGGCGTTGGAACAGTATTAGGGAGGCTTTTTGGCGGGGCGATTCAGGGACTGATTCAGAATTACATAAACTTTGGTGGCACGCTGTGTTCCTGGGGGCC‑GAAAATCGCACTTGGGATTTTGTTGTTTATTTTTATAGCATTAAAGCGTGGAt                                                             >  1:355442/1‑251 (MQ=255)
                                                                                                                                                                                      cGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAAc                                                         <  2:292346/250‑1 (MQ=255)
                                                                                                                                                                                                 cAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAAATGGATGCTATCGTCTCCGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGGATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTTATGGCACGCTGAGTTCCTTGGGGAC‑GAAAATCGCCATCGGTATTTTGTTTTTTATTTTTATAGCATTACAGCGTGGGTTAACGGTGCTGTgggg                                             >  2:490235/1‑250 (MQ=255)
                                                                                                                                                                                                 cAGGCCGGATATGCGCTGGCGGGCGTAGGGGCGGAACTGGATGGTATCGCCTCAGGGGT‑AATTGGCGGTAAGGTTTTG‑AGCGGTGGCGTTTGAACGGGATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGAATTTCATAAAATTTGTTGGCACGCTGGGTTCCTGGGGGACTGCAAA‑CGCCATCGGGATTTTGTTGTTTATTTTTATTGCGTTTCAGCGTGGATTCACggggttgggggg                                             >  2:441857/1‑241 (MQ=255)
                                                                                                                                                                                                         atatGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTTAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTTTTGTTTATTTTTATAGCATTACAGCGTGGGTTAAAGGTGCTGGGGGAGAATCGTc                                     >  1:294617/1‑251 (MQ=255)
                                                                                                                                                                                                              cgcTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGGCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGAGTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTAGTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGt                                <  2:105040/251‑1 (MQ=255)
                                                                                                                                                                                                                     gggcgTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGcc                           >  1:385535/1‑249 (MQ=255)
                                                                                                                                                                                                                     gggcgTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGcc                           >  2:487049/1‑249 (MQ=255)
                                                                                                                                                                                                                       gcgTAGGTGTGGAACTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTg                       <  1:92700/251‑1 (MQ=255)
                                                                                                                                                                                                                                   aaCTGGATGCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAAc           >  2:32004/1‑251 (MQ=255)
                                                                                                                                                                                                                                           gCTATCGTCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTgcgc    >  1:520403/1‑250 (MQ=255)
                                                                                                                                                                                                                                              aTCGTCTCAGTGGT‑AAGTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGTGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGTCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGTGCGAATCTTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCgcag  <  2:99724/249‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       
GATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCATTTATATGCTCTCCACCGGACTGGCAACGCTGGCGGGGATTGTCTTCTCGATTTATACCCAGGCCGGATATGCGCTGGCGGGCGTAGGTGTGGAACTGGATGCTATCGCCTCAGTGGT‑AATTGGCGGTACGCTTTTG‑AGCGGTGGCGTTGGAACGGTATTAGGGACGCTTTTTGGCGTGGCGATTCAGGGACTGATTCAGACTTACATAAACTTTGATGGCACGCTGAGTTCCTGGTGGAC‑GAAAATCGCCATCGGTATTTTGTTGTTTATTTTTATAGCATTACAGCGTGGATTAACGGTGCTGTGGGAGAATCGTCAGAGTTCGCCAGTGACAAGAGTCAACATTGCGCAG  >  NC_000913/4454086‑4454572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑