Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,792,138	G→A	intergenic (‑118/+129)	rflP ← / ← nlpC	anti‑FlhDC factor RflP/NlpC/P60 family lipoprotein NlpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,792,138	0	G	A	100.0%	60.0 / NA	20	intergenic (‑118/+129)	rflP/nlpC	anti‑FlhDC factor RflP/NlpC/P60 family lipoprotein NlpC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/13);  total (7/13)

CGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCT  >  NC_000913/1791901‑1792341                                                                                                                                                                                                                                              |                                                                                                                                                                                                            cGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCttttttt                                                                                                                                                                                                >  1:185555/1‑251 (MQ=255)  gTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTGTCAAGGGTCCTTTTTTTa                                                                                                                                                                                               <  1:582530/251‑1 (MQ=255)                      aTGGGTAATTAACTCAACGCCGAAAAGGACTTTCTGGTTGTCTCTTATCGGCAGGAAATAGCAAGCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCTTCCCCCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGATTAACTTTCATTTGTTCCATTTTAACCCCTTTTTCAAGGGTCCTTTTTT‑AGATTATCCTGATTATAAACGa                                                                                                                                                                          <  2:391438/251‑1 (MQ=255)                             aTTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATc                                                                                                                                                                    <  1:352339/251‑1 (MQ=255)                                    cAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTAc                                                                                                                                                             >  1:297898/1‑251 (MQ=255)                                                             gTCTCTTATCGGCAGTAAATAGGAATCAGAATGATAAAGATTCTCCAAAAAAATCTGCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAATACATAACAATTACGGAAAGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCGTTTTTTTGGATTATCTTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAAtacta                                                                                                                                     <  1:594657/250‑1 (MQ=255)                                                                               aTAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATg                                                                                                                  <  1:540089/251‑1 (MQ=255)                                                                                    caTCAGAATGATAAAGATTCTCCAAAAACATCTTCATGGTAACCGTACCTCTCGAAGGATGTTGTTCAGTATCCGGCTTAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCGGATGATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAAGACTACTGTATTCAGGAGTAAATTGACgg                                                                                                             <  2:35885/250‑1 (MQ=255)                                                                                            tGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCTGATtata                                                                                                                                                                               <  1:39089/177‑1 (MQ=255)                                                                                            tGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCTGATtata                                                                                                                                                                               >  2:39089/1‑177 (MQ=255)                                                                                                aaaGATGCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTatcatc                                                                                                 <  1:202312/251‑1 (MQ=255)                                                                                                                               cGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGtc                                                                  >  2:115944/1‑251 (MQ=255)                                                                                                                                         gAAGGATGGTATTTAGTTTCCGGCTAACAGGGATGAGTCCCTAAGACAGATCAATTACAGTAGGAGCAACTTTCATTTGTTCCATGCTAACGACTTTTTCAAGGGTCGTTTTTATAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTGTGTCTGCAAATTACTACTTTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTTGATTCGTCTCGCTTGcc                                                         >  2:445953/1‑250 (MQ=255)                                                                                                                                            ggATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTGTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGaa                                                     <  1:146832/251‑1 (MQ=255)                                                                                                                                                  ttttCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAttttt                                               >  1:346849/1‑251 (MQ=255)                                                                                                                                                                cTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTa                                                                                                                                                              >  1:211532/1‑126 (MQ=255)                                                                                                                                                                cTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTa                                                                                                                                                              <  2:211532/126‑1 (MQ=255)                                                                                                                                                                     aGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCa                            <  2:200480/251‑1 (MQ=255)                                                                                                                                                                                            cAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCtt     <  2:534597/251‑1 (MQ=255)                                                                                                                                                                                                tACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAAGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCt  <  1:277539/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                            CGTACCATCTTCACTGGAGAAATGGGTAATTAACTCAACGCCGACAAGGACTTGCTGGTTGTCTCTTATCGGCAGGAAATAGCAATCAGAATGATAAAGATTCTCCAAAAAAATCTTCATGGTAACCGTCCCTCTCGAAGGATGTTTTTCAGTATCCGGCTAAAAGGGATGAGGCCATAAGACATAACAATTACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCT  >  NC_000913/1791901‑1792341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑