Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	759,426	G→A	E241K (GAG→AAG)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	759,426	0	G	A	97.1%	213.1 / ‑4.0	68	E241K (GAG→AAG)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (40/26);  total (40/28)
Fisher's exact test for biased strand distribution p-value = 1.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCA  >  NC_000913/759182‑759670                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ccccAAGCAAACCTGCGGCGGCCCGATTGGTGCCGGGTATAGGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTACCGAGTCTGGTCGCGCGCCTTTCAATAGCGAAGAAAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAgatga                                                                                                                                                                                                                                                <  2:636567/248‑1 (MQ=255)    aaaGCAAACCACCTGCGGCCCGATGGGTGCCGAGTATATGCCCATGCCCAGAACCGAAGAAAAACGCTGTATCCAACAGCTGAGCGAGTCTGGTCGCGCGACTTTCAATATCGAGGAGAAAAAACGCTCCTTAGCCAAACGGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATcc                                                                                                                                                                                                                                             <  2:213962/250‑1 (MQ=255)      aGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGc                                                                                                                                                                                                                                           <  1:466037/251‑1 (MQ=255)           aCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACAAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGc                                                                                                                                                                                                                                      >  1:275618/1‑251 (MQ=255)               aCTGCGGCCCGATTGGTGCCGAGTATATGCACATTCCCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAACCGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTTCCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTg                                                                                                                                                                                                                                    <  1:365545/249‑1 (MQ=255)               aCTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGc                                                                                                                                                                                                                                  >  1:73714/1‑251 (MQ=255)                        cGATTGGTGCCGAGTATATGCACATTACCAGCCCCAAAAAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGGCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGc                                                                                                                                                                                                                         <  1:68807/251‑1 (MQ=255)                                   gAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAgg                                                                                                                                                                                                              >  1:636731/1‑250 (MQ=255)                                         atGCACATTCCCAGCCCAGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCGGGTCGCGCGACTTTAAATAGCAAAGAGAAAAAACGCTTCTTAAGCAAACTGCCCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCAc                                                                                                                                                                                                                                        <  2:246667/219‑1 (MQ=255)                                         atGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCAc                                                                                                                                                                                                                                        >  1:246667/1‑219 (MQ=255)                                         atGCACATTACAAGAACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATACCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGNtcc                                                                                                                                                                                                        >  2:606163/1‑250 (MQ=255)                                              caTTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGACGTGGTTCTCggg                                                                                                                                                                                                    >  2:251056/1‑250 (MQ=255)                                               aTTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGAt                                                                                                                                                                                                  >  2:478436/1‑251 (MQ=255)                                                 tACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGAGACTTTAAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATgg                                                                                                                                                                                                >  1:32994/1‑251 (MQ=255)                                                      acACCGAAGAAAAACGCAGGACCCAACGGCGAATCGAGTCTGGTCGCGCGACTTTCAATATCGAAGAGAAAAAACGCATCTTAAGCTAACGGACCGCCGCTGAAGGTCTTGAACGTTACCACGGCGGAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAGTCCCGATGCTTAAAAAGATGATCCGCCACGCTGTCGACAGCGGCACCCGCGGAGTGGTTCTCGGGATGGCGCAc                                                                                                                                                                                           <  2:433306/250‑1 (MQ=255)                                                              gaaAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATGGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGgtcg                                                                                                                                                                                   <  1:551666/251‑1 (MQ=255)                                                                            ccAACAGCGTATCGAGTCTGGTCGCGCGACTTTAAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCtgaacgtgctg                                                                                                                                                                      <  1:427387/250‑1 (MQ=255)                                                                              aaCAGCATATCGAGTATGATAGCGCGACTTTACATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCGGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGCGGTCGTCtgaacgtgctggtg                                                                                                                                                                   >  1:245624/1‑251 (MQ=255)                                                                                    gTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCtgaacgtgctggtgaacgtg                                                                                                                                                             >  1:464903/1‑251 (MQ=255)                                                                                      aTCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCtgaacgtgctggtgaacgtgct                                                                                                                                                           >  1:20920/1‑251 (MQ=255)                                                                                            tCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTa                                                                                                                                                      >  1:438491/1‑250 (MQ=255)                                                                                                  cgcgcgACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGc                                                                                                                                                                                                                                           >  2:249123/1‑159 (MQ=255)                                                                                                  cgcgcgACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGc                                                                                                                                                                                                                                           <  1:249123/159‑1 (MQ=255)                                                                                                               ataaagAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAAAGCTTCTCGCTGGATGGCGGTGACGCGTTAATACCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGAGAAGTGGTTCACGGGATGGCGCACCGTGGTCGTCTGAACGAGCGGGTGAACGTGCTGGGTAAAAAACCGCAGGACTTGTTc                                                                                                                                  >  2:504275/6‑251 (MQ=255)                                                                                                               aTAGCGAAGAGAAAAAACGCTTCGTAAACGAAGTGACCTCCGCTGAAGGCCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAATACGCTTCTCGCGGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCGGGTGAACGTGCTGTGTAAGAATCCTCAATACTTGTTc                                                                                                                                  <  2:619210/251‑1 (MQ=255)                                                                                                                             aaaCGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGCGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCgg                                                                                                                    <  1:566163/251‑1 (MQ=255)                                                                                                                               aCGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGCACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGt                                                                                                                   >  1:481593/1‑250 (MQ=255)                                                                                                                                  cttcttAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGATTTCGCCGGTaacc                                                                                                               >  1:601303/1‑249 (MQ=255)                                                                                                                                   ttcttAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAAc                                                                                                               >  1:65780/1‑250 (MQ=255)                                                                                                                                   ttcttAAGCAAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAACATTCCCTGGCGCAAAAGGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGAACCGTGGTCGTCTGAACGTGCTGGTGACCGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTaaaac                                                                                                              >  2:227985/1‑249 (MQ=255)                                                                                                                                         aGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTCCCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGCTGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATaa                                                                                                           >  2:133044/1‑248 (MQ=255)                                                                                                                                            gAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAac                                                                                                      >  2:218009/1‑250 (MQ=255)                                                                                                                                            gAACGGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAaca                                                                                                     <  2:448432/251‑1 (MQ=255)                                                                                                                                              aCTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACAcc                                                                                                   >  2:575811/1‑251 (MQ=255)                                                                                                                                              aCTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACAcc                                                                                                   >  1:1127/1‑251 (MQ=255)                                                                                                                                                tGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCACCATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCACGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTc                                                                                                 >  2:78944/1‑251 (MQ=255)                                                                                                                                                         cTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGAAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCACAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACgggg                                                                                        >  2:636785/1‑250 (MQ=255)                                                                                                                                                           gAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCTCTGGCAGGAGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGCGGTTCTCGGGACGGCGCACCGTGGTCGTCTGAACGTGCTGAGGACCGTGCAGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATACAGAAAACCTCAGCACGGGTg                                                                                       >  1:409429/1‑250 (MQ=255)                                                                                                                                                           gAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAAGCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGt                                                                                        <  2:486695/249‑1 (MQ=255)                                                                                                                                                             aGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCGTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGTGTGACg                                                                                    <  2:521028/251‑1 (MQ=255)                                                                                                                                                                          tACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACcaca                                                                        <  2:656968/250‑1 (MQ=255)                                                                                                                                                                           aCCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACCTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACAt                                                                       <  1:378741/250‑1 (MQ=255)                                                                                                                                                                               cGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCt                                                                  >  1:246889/1‑251 (MQ=255)                                                                                                                                                                                 gcgcAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTtc                                                                <  1:78944/251‑1 (MQ=255)                                                                                                                                                                                    cAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCg                                                             >  2:250950/1‑251 (MQ=255)                                                                                                                                                                                     aaaaTTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGt                                                            >  2:178704/1‑251 (MQ=255)                                                                                                                                                                                       aaTTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTGCTCGTCt                                                          >  2:155554/1‑251 (MQ=255)                                                                                                                                                                                        aTTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCGCCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTg                                                         <  2:275618/251‑1 (MQ=255)                                                                                                                                                                                                 cgcNAACCGATTCTCGCCGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGTCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTCGTTCGACTTGTTCGCCGGCAAACATAAAGAACTCCTCAGCCCGTGTGACGTGAAATACCACATGTGCTTCTCGTCTGACTTCCAGa                                                <  2:593610/251‑1 (MQ=255)                                                                                                                                                                                                 cgcAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATACAGAACACCTCAGCACGGGTGACGTGAAATACCACATGAGCTTCTCGTCTGACTTcc                                                   >  2:507726/1‑248 (MQ=255)                                                                                                                                                                                                    aaaaCGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCg                                             >  2:564868/1‑251 (MQ=255)                                                                                                                                                                                                      aaCGCTTCTCGCTGGAAGGCGGTGACTCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACATCGGCACCCGCGAAATGGTTCTCGTGATGGCGCCCCGTGGTCTTCTGAACGTTCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCGCGGCACGGGTGACGTGAAATACCACATGTGCTTCTCGTCTGACTTCCAGACCGAt                                           <  1:459347/251‑1 (MQ=255)                                                                                                                                                                                                        cGCTTCTCGCTGGAAGGCGGTGACGCGATAATCCCGATGCTTAAAAAGATGATCCGCTACGCTGGCAACAGCGGCACTCGCGAAGAGGTTCTCGGCAAGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCACAGCATGGGGGAGGTGAATTACCACCGGGGCTTCTCGTCTGCATTCACGACCGATgg                                         >  2:75986/1‑251 (MQ=255)                                                                                                                                                                                                               cGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTg                                  >  1:320117/1‑251 (MQ=255)                                                                                                                                                                                                                   ggAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGc                              >  2:658739/1‑251 (MQ=255)                                                                                                                                                                                                                   ggAAGGCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCGGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGt                                <  1:636785/249‑1 (MQ=255)                                                                                                                                                                                                                       ggCGGTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACct                          <  1:250950/251‑1 (MQ=255)                                                                                                                                                                                                                          ggTGACGCGTTAATCCCGCTGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATaaa                                                                                                          >  2:444985/1‑168 (MQ=255)                                                                                                                                                                                                                          ggTGACGCGTTAATCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCGGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATaaa                                                                                                          <  1:444985/168‑1 (MQ=255)                                                                                                                                                                                                                                     aaTCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTa            <  2:1127/251‑1 (MQ=255)                                                                                                                                                                                                                                      aTCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTa            >  2:550626/1‑250 (MQ=255)                                                                                                                                                                                                                                       tCCCGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAc          >  1:24306/1‑251 (MQ=255)                                                                                                                                                                                                                                        cccGATGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAc          <  1:266756/250‑1 (MQ=255)                                                                                                                                                                                                                                           caTGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGCCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTTTTCGACGAGTTCGCCGGTAAACATAAAGAACTCCTCAGCGCGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCTCCTGGCGCTGACGTTTAACCCGt      <  2:245624/250‑1 (MQ=255)                                                                                                                                                                                                                                            aTGCTTAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGtc     >  1:158191/1‑251 (MQ=255)                                                                                                                                                                                                                                               cTTAAAAAGTTGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCCGGATGGCGCACCGTGGCCGTCTGACCGTGCTGGCGAACGTGCTGCGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGCTAAACATAAAGAACACCTCAGCACGGGAGACGTGAAATACCACATGGACTTCTCGTCGGACTTCCAGACCGATGGCGGCCTGGCGCACCTGGCGCTGGCGTTTAACCCGtcgcc  >  2:149958/1‑248 (MQ=255)                                                                                                                                                                                                                                                 tAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAccc        <  1:252285/243‑1 (MQ=255)                                                                                                                                                                                                                                                 tAAAAAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCAGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAccc        >  2:252285/1‑243 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     CCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCGCTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCGCCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCA  >  NC_000913/759182‑759670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑